Restriction map for
pFN21AB5329
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MspA1I MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 421 CGCG CG^CG
AclI 37 AACGTT AA^CGTT
AfaI 81 GTAC GT^AC
AluI 77 AGCT AG^CT
AlwI 183 GATCC GGATC(4/5)
AvaII 450 GGACC G^G[AT]CC
BalI 299 TGGCCA TGG^CCA
BpmI 73 CTGGAG CTGGAG(16/14)
BsaI 49 GAGACC GGTCTC(1/5)
BsmAI 49 GAGAC GTCTC(1/5)
BsmFI 205 GGGAC GGGAC(10/14)
BspHI 179 TCATGA T^CATGA
BsrDI 295 GCAATG GCAATG(2/0)
BstEII 193 GGTGACC G^GT[ACGT]ACC
BstF5I 259 CATCC GGATG(2/0)
BstPI 193 GGTGACC G^GT[ACGT]ACC
BstUI 421 CGCG CG^CG
BstXI 16 CCAGATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 90 GCATGC GC[ACGT]^[ACGT]GC
DdeI 119 CTGAG C^T[ACGT]AG
DraIII 354 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 193 GGTGACC G^GT[ACGT]ACC
EcoRI 287 GAATTC G^AATTC
EcoT14I 186 CCAAGG C^C[AT][AT]GG
FauI 307 GCGGG CCCGC(4/6)
FokI 259 CATCC GGATG(9/13)
MluNI 299 TGGCCA TGG^CCA
MscI 299 TGGCCA TGG^CCA
MseI 479 TTAA T^TAA
MslI 405 CATAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 421 CGCG CG^CG
MwoI 118 GCTGAGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 309 GGGGCC GG[ACGT]^[ACGT]CC
NspI 90 GCATGC [GA]CATG^[CT]
PflMI 353 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 37 AACGTT AA^CGTT
RcaI 179 TCATGA T^CATGA
RsaI 81 GTAC GT^AC
SfaNI 223 GCATC GCATC(5/9)
SphI 90 GCATGC GCATG^C
StyI 186 CCAAGG C^C[AT][AT]GG
Tru9I 479 TTAA T^TAA
TspRI 358 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 353 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 35 CCAACGTTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbvI 27 GCAGC GCAGC(8/12)
326 GCAGC
BsaJI 186 CCAAGG C^C[ACGT][ACGT]GG
446 CCCAGG
BssKI 23 CCTGG ^CC[ACGT]GG
447 CCAGG
BstNI 23 CCTGG CC^[AT]GG
447 CCAGG
Cfr13I 310 GGGCC G^G[ACGT]CC
450 GGACC
DpnII 183 GATC ^GATC
474 GATC
EaeI 299 TGGCCA [CT]^GGCC[GA]
387 TGGCCG
EcoRII 23 CCTGG ^CC[AT]GG
447 CCAGG
HphI 193 GGTGA GGTGA(8/7)
414 GGTGA
MaeII 38 ACGT A^CGT
467 ACGT
MboI 183 GATC ^GATC
474 GATC
MboII 247 GAAGA GAAGA(8/7)
471 GAAGA
MvaI 23 CCTGG CC^[AT]GG
447 CCAGG
NdeII 183 GATC ^GATC
474 GATC
Sau3AI 183 GATC ^GATC
474 GATC
Sau96I 310 GGGCC G^G[ACGT]CC
450 GGACC
ScrFI 23 CCTGG CC^[ACGT]GG
447 CCAGG
TaqI 132 TCGA T^CGA
213 TCGA
TseI 27 GCAGC G^C[AT]GC
326 GCAGC
Tsp45I 194 GTGAC ^GT[GC]AC
340 GTGAC
TthHB8I 132 TCGA T^CGA
213 TCGA
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AciI AcsI ApoI BsiYI BslI
BsrI Fnu4HI HaeIII HinfI ItaI
MaeIII MnlI NlaIII TfiI Tsp509I