(Linear) (Six-base) MAPSORT of: KIBB5329.seq  Check: 6549  from: 1  to: 498



KIBB5329.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AclI AA'CG_TT

Cuts at:      0      38     498 
   Size:         38     460
 
ApoI r'AATT_y

Cuts at:      0     154     271     287     498 
   Size:        154     117      16     211

  Fragments arranged by size: 

                 211     154     117      16
 
BmgBI CAC'GTC

Cuts at:      0     468     498 
   Size:        468      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     351     498 
   Size:        351     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     498 
   Size:         94     404
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     498 
   Size:         43     455
 
BspHI T'CATG_A

Cuts at:      0     179     498 
   Size:        179     319
 
BsrDI GCAATG_nn'

Cuts at:      0     302     498 
   Size:        302     196
 
BstEII G'GTnAC_C

Cuts at:      0     193     498 
   Size:        193     305
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      23     498 
   Size:         23     475
 
DraIII CAC_nnn'GTG

Cuts at:      0     359     498 
   Size:        359     139
 
EaeI y'GGCC_r

Cuts at:      0     299     387     498 
   Size:        299      88     111

  Fragments arranged by size: 

                 299     111      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     498 
   Size:         94     404
 
EcoRI G'AATT_C

Cuts at:      0     287     498 
   Size:        287     211
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     271     498 
   Size:        271     227
 
MscI TGG'CCA

Cuts at:      0     301     498 
   Size:        301     197
 
MslI CAynn'nnrTG

Cuts at:      0     409     498 
   Size:        409      89
 
NspI r_CATG'y

Cuts at:      0      94     498 
   Size:         94     404
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     359     498 
   Size:        359     139
 
SphI G_CATG'C

Cuts at:      0      94     498 
   Size:         94     404
 
StyI C'CwwG_G

Cuts at:      0     186     498 
   Size:        186     312
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      48     370     498 
   Size:         48     322     128

  Fragments arranged by size: 

                 322     128      48
 
TatI w'GTAC_w

Cuts at:      0      80     498 
   Size:         80     418
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      53      85     498 
   Size:         53      32     413

  Fragments arranged by size: 

                 413      53      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     498 
   Size:         42     456
 

 Enzymes that do cut:

     AclI     ApoI    BmgBI     BmrI     BpmI     BsaI    BspHI    BsrDI
   BstEII    BstXI   DraIII     EaeI  Eco57MI    EcoRI     FalI     MscI
     MslI     NspI    PflMI     SphI     StyI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI