(Linear) (Six-base) MAPSORT of: KIEE1365.seq Check: 5280 from: 1 to: 1170 KIEE1365.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 670 1170 Size: 670 500 AatII G_ACGT'C Cuts at: 0 812 1170 Size: 812 358 AccI GT'mk_AC Cuts at: 0 119 1170 Size: 119 1051 AfeI AGC'GCT Cuts at: 0 49 1072 1170 Size: 49 1023 98 Fragments arranged by size: 1023 98 49 AflIII A'CryG_T Cuts at: 0 77 857 1170 Size: 77 780 313 Fragments arranged by size: 780 313 77 AhdI GACnn_n'nnGTC Cuts at: 0 732 1170 Size: 732 438 AleI CACnn'nnGTG Cuts at: 0 715 1170 Size: 715 455 AlwNI CAG_nnn'CTG Cuts at: 0 102 1170 Size: 102 1068 AvaI C'yCGr_G Cuts at: 0 510 628 742 1036 1048 1170 Size: 510 118 114 294 12 122 Fragments arranged by size: 510 294 122 118 114 12 BamHI G'GATC_C Cuts at: 0 611 1170 Size: 611 559 BanI G'GyrC_C Cuts at: 0 891 1092 1170 Size: 891 201 78 Fragments arranged by size: 891 201 78 BanII G_rGCy'C Cuts at: 0 517 635 671 1036 1045 1170 Size: 517 118 36 365 9 125 Fragments arranged by size: 517 365 125 118 36 9 BbeI G_GCGC'C Cuts at: 0 895 1170 Size: 895 275 BbsI GAAGACnn'nnnn_ Cuts at: 0 511 665 1170 Size: 511 154 505 Fragments arranged by size: 511 505 154 BbvCI CC'TCA_GC Cuts at: 0 955 1170 Size: 955 215 BglI GCCn_nnn'nGGC Cuts at: 0 703 1101 1170 Size: 703 398 69 Fragments arranged by size: 703 398 69 BglII A'GATC_T Cuts at: 0 137 320 1170 Size: 137 183 850 Fragments arranged by size: 850 183 137 Bme1580I G_kGCm'C Cuts at: 0 277 1097 1170 Size: 277 820 73 Fragments arranged by size: 820 277 73 BmgBI CAC'GTC Cuts at: 0 543 858 1170 Size: 543 315 312 Fragments arranged by size: 543 315 312 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 525 1170 Size: 525 645 Bpu10I CC'TnA_GC Cuts at: 0 665 955 1170 Size: 665 290 215 Fragments arranged by size: 665 290 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 1170 Size: 358 812 BsaHI Gr'CG_yC Cuts at: 0 809 892 965 1005 1159 1170 Size: 809 83 73 40 154 11 Fragments arranged by size: 809 154 83 73 40 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 331 1066 1069 1170 Size: 331 735 3 101 Fragments arranged by size: 735 331 101 3 BseYI C'CCAG_C Cuts at: 0 33 58 225 615 1170 Size: 33 25 167 390 555 Fragments arranged by size: 555 390 167 33 25 BsiEI CG_ry'CG Cuts at: 0 943 1021 1105 1170 Size: 943 78 84 65 Fragments arranged by size: 943 84 78 65 BsiHKAI G_wGCw'C Cuts at: 0 1144 1170 Size: 1144 26 BsmBI CGTCTCn'nnnn_ Cuts at: 0 646 1170 Size: 646 524 Bsp1286I G_dGCh'C Cuts at: 0 277 517 635 671 1036 1045 1097 1144 Size: 277 240 118 36 365 9 52 47 Cuts at: 1144 1170 Size: 26 Fragments arranged by size: 365 277 240 118 52 47 36 26 9 BspHI T'CATG_A Cuts at: 0 302 1170 Size: 302 868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 670 845 893 1170 Size: 670 175 48 277 Fragments arranged by size: 670 277 175 48 BsrBI CCG'CTC Cuts at: 0 572 1047 1059 1170 Size: 572 475 12 111 Fragments arranged by size: 572 475 111 12 BsrDI GCAATG_nn' Cuts at: 0 488 1170 Size: 488 682 BsrFI r'CCGG_y Cuts at: 0 122 457 1086 1170 Size: 122 335 629 84 Fragments arranged by size: 629 335 122 84 BsrGI T'GTAC_A Cuts at: 0 74 1170 Size: 74 1096 BstYI r'GATC_y Cuts at: 0 137 320 611 1170 Size: 137 183 291 559 Fragments arranged by size: 559 291 183 137 BtgI C'CryG_G Cuts at: 0 383 533 1099 1170 Size: 383 150 566 71 Fragments arranged by size: 566 383 150 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 9 1170 Size: 9 1161 BtsI GCAGTG_nn' Cuts at: 0 710 1170 Size: 710 460 DraIII CAC_nnn'GTG Cuts at: 0 72 1170 Size: 72 1098 EaeI y'GGCC_r Cuts at: 0 1018 1102 1170 Size: 1018 84 68 Fragments arranged by size: 1018 84 68 EagI C'GGCC_G Cuts at: 0 1018 1102 1170 Size: 1018 84 68 Fragments arranged by size: 1018 84 68 EarI CTCTTCn'nnn_ Cuts at: 0 556 1170 Size: 556 614 EcoNI CCTnn'n_nnAGG Cuts at: 0 742 958 1170 Size: 742 216 212 Fragments arranged by size: 742 216 212 EcoO109I rG'GnC_Cy Cuts at: 0 99 1170 Size: 99 1071 HaeII r_GCGC'y Cuts at: 0 51 129 895 954 1074 1112 1170 Size: 51 78 766 59 120 38 58 Fragments arranged by size: 766 120 78 59 58 51 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 596 628 718 750 1170 Size: 596 32 90 32 420 Fragments arranged by size: 596 420 90 32 32 KasI G'GCGC_C Cuts at: 0 891 1170 Size: 891 279 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 86 1170 Size: 86 1084 MslI CAynn'nnrTG Cuts at: 0 301 715 1170 Size: 301 414 455 Fragments arranged by size: 455 414 301 MspA1I CmG'CkG Cuts at: 0 252 329 939 1101 1130 1170 Size: 252 77 610 162 29 40 Fragments arranged by size: 610 252 162 77 40 29 NaeI GCC'GGC Cuts at: 0 1088 1170 Size: 1088 82 NarI GG'CG_CC Cuts at: 0 892 1170 Size: 892 278 NgoMIV G'CCGG_C Cuts at: 0 1086 1170 Size: 1086 84 NspI r_CATG'y Cuts at: 0 81 1170 Size: 81 1089 PciI A'CATG_T Cuts at: 0 77 1170 Size: 77 1093 PfoI T'CCnGG_A Cuts at: 0 776 1170 Size: 776 394 PpuMI rG'GwC_Cy Cuts at: 0 99 1170 Size: 99 1071 PspXI vC'TCGA_Gb Cuts at: 0 628 742 1048 1170 Size: 628 114 306 122 Fragments arranged by size: 628 306 122 114 PstI C_TGCA'G Cuts at: 0 295 476 998 1135 1170 Size: 295 181 522 137 35 Fragments arranged by size: 522 295 181 137 35 SacII CC_GC'GG Cuts at: 0 1102 1170 Size: 1102 68 SapI GCTCTTCn'nnn_ Cuts at: 0 556 1170 Size: 556 614 ScaI AGT'ACT Cuts at: 0 289 1170 Size: 289 881 SfcI C'TryA_G Cuts at: 0 291 472 994 1131 1170 Size: 291 181 522 137 39 Fragments arranged by size: 522 291 181 137 39 SfoI GGC'GCC Cuts at: 0 893 1170 Size: 893 277 SmaI CCC'GGG Cuts at: 0 1038 1170 Size: 1038 132 SmlI C'TyrA_G Cuts at: 0 373 628 742 1048 1170 Size: 373 255 114 306 122 Fragments arranged by size: 373 306 255 122 114 StuI AGG'CCT Cuts at: 0 706 1170 Size: 706 464 TatI w'GTAC_w Cuts at: 0 74 287 1170 Size: 74 213 883 Fragments arranged by size: 883 213 74 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 772 804 1170 Size: 772 32 366 Fragments arranged by size: 772 366 32 Tth111I GACn'n_nGTC Cuts at: 0 808 1170 Size: 808 362 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 233 540 1152 1170 Size: 233 307 612 18 Fragments arranged by size: 612 307 233 18 XhoI C'TCGA_G Cuts at: 0 628 742 1048 1170 Size: 628 114 306 122 Fragments arranged by size: 628 306 122 114 XmaI C'CCGG_G Cuts at: 0 1036 1170 Size: 1036 134 XmnI GAAnn'nnTTC Cuts at: 0 49 1170 Size: 49 1121 ZraI GAC'GTC Cuts at: 0 810 1170 Size: 810 360 Enzymes that do cut: AarI AatII AccI AfeI AflIII AhdI AleI AlwNI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI BplI Bpu10I BpuEI BsaHI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoNI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PpuMI PspXI PstI SacII SapI ScaI SfcI SfoI SmaI SmlI StuI TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AcuI AflII AgeI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BpmI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BspEI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII XbaI