Restriction map for pFN21AE1365
 (Linear) (Six-base) MAPSORT of: KIEE1365.seq  Check: 5280  from: 1  to: 1170



KIEE1365.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     670    1170 
   Size:        670     500
 
AatII G_ACGT'C

Cuts at:      0     812    1170 
   Size:        812     358
 
AccI GT'mk_AC

Cuts at:      0     119    1170 
   Size:        119    1051
 
AfeI AGC'GCT

Cuts at:      0      49    1072    1170 
   Size:         49    1023      98

  Fragments arranged by size: 

                1023      98      49
 
AflIII A'CryG_T

Cuts at:      0      77     857    1170 
   Size:         77     780     313

  Fragments arranged by size: 

                 780     313      77
 
AhdI GACnn_n'nnGTC

Cuts at:      0     732    1170 
   Size:        732     438
 
AleI CACnn'nnGTG

Cuts at:      0     715    1170 
   Size:        715     455
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102    1170 
   Size:        102    1068
 
AvaI C'yCGr_G

Cuts at:      0     510     628     742    1036    1048    1170 
   Size:        510     118     114     294      12     122

  Fragments arranged by size: 

                 510     294     122     118     114      12
 
BamHI G'GATC_C

Cuts at:      0     611    1170 
   Size:        611     559
 
BanI G'GyrC_C

Cuts at:      0     891    1092    1170 
   Size:        891     201      78

  Fragments arranged by size: 

                 891     201      78
 
BanII G_rGCy'C

Cuts at:      0     517     635     671    1036    1045    1170 
   Size:        517     118      36     365       9     125

  Fragments arranged by size: 

                 517     365     125     118      36       9
 
BbeI G_GCGC'C

Cuts at:      0     895    1170 
   Size:        895     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     511     665    1170 
   Size:        511     154     505

  Fragments arranged by size: 

                 511     505     154
 
BbvCI CC'TCA_GC

Cuts at:      0     955    1170 
   Size:        955     215
 
BglI GCCn_nnn'nGGC

Cuts at:      0     703    1101    1170 
   Size:        703     398      69

  Fragments arranged by size: 

                 703     398      69
 
BglII A'GATC_T

Cuts at:      0     137     320    1170 
   Size:        137     183     850

  Fragments arranged by size: 

                 850     183     137
 
Bme1580I G_kGCm'C

Cuts at:      0     277    1097    1170 
   Size:        277     820      73

  Fragments arranged by size: 

                 820     277      73
 
BmgBI CAC'GTC

Cuts at:      0     543     858    1170 
   Size:        543     315     312

  Fragments arranged by size: 

                 543     315     312
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     525    1170 
   Size:        525     645
 
Bpu10I CC'TnA_GC

Cuts at:      0     665     955    1170 
   Size:        665     290     215

  Fragments arranged by size: 

                 665     290     215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358    1170 
   Size:        358     812
 
BsaHI Gr'CG_yC

Cuts at:      0     809     892     965    1005    1159    1170 
   Size:        809      83      73      40     154      11

  Fragments arranged by size: 

                 809     154      83      73      40      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     331    1066    1069    1170 
   Size:        331     735       3     101

  Fragments arranged by size: 

                 735     331     101       3
 
BseYI C'CCAG_C

Cuts at:      0      33      58     225     615    1170 
   Size:         33      25     167     390     555

  Fragments arranged by size: 

                 555     390     167      33      25
 
BsiEI CG_ry'CG

Cuts at:      0     943    1021    1105    1170 
   Size:        943      78      84      65

  Fragments arranged by size: 

                 943      84      78      65
 
BsiHKAI G_wGCw'C

Cuts at:      0    1144    1170 
   Size:       1144      26
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     646    1170 
   Size:        646     524
 
Bsp1286I G_dGCh'C

Cuts at:      0     277     517     635     671    1036    1045    1097    1144 
   Size:        277     240     118      36     365       9      52      47

Cuts at:   1144    1170 
   Size:         26

  Fragments arranged by size: 

                 365     277     240     118      52      47      36      26
                   9
 
BspHI T'CATG_A

Cuts at:      0     302    1170 
   Size:        302     868
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     670     845     893    1170 
   Size:        670     175      48     277

  Fragments arranged by size: 

                 670     277     175      48
 
BsrBI CCG'CTC

Cuts at:      0     572    1047    1059    1170 
   Size:        572     475      12     111

  Fragments arranged by size: 

                 572     475     111      12
 
BsrDI GCAATG_nn'

Cuts at:      0     488    1170 
   Size:        488     682
 
BsrFI r'CCGG_y

Cuts at:      0     122     457    1086    1170 
   Size:        122     335     629      84

  Fragments arranged by size: 

                 629     335     122      84
 
BsrGI T'GTAC_A

Cuts at:      0      74    1170 
   Size:         74    1096
 
BstYI r'GATC_y

Cuts at:      0     137     320     611    1170 
   Size:        137     183     291     559

  Fragments arranged by size: 

                 559     291     183     137
 
BtgI C'CryG_G

Cuts at:      0     383     533    1099    1170 
   Size:        383     150     566      71

  Fragments arranged by size: 

                 566     383     150      71
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       9    1170 
   Size:          9    1161
 
BtsI GCAGTG_nn'

Cuts at:      0     710    1170 
   Size:        710     460
 
DraIII CAC_nnn'GTG

Cuts at:      0      72    1170 
   Size:         72    1098
 
EaeI y'GGCC_r

Cuts at:      0    1018    1102    1170 
   Size:       1018      84      68

  Fragments arranged by size: 

                1018      84      68
 
EagI C'GGCC_G

Cuts at:      0    1018    1102    1170 
   Size:       1018      84      68

  Fragments arranged by size: 

                1018      84      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     556    1170 
   Size:        556     614
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     742     958    1170 
   Size:        742     216     212

  Fragments arranged by size: 

                 742     216     212
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99    1170 
   Size:         99    1071
 
HaeII r_GCGC'y

Cuts at:      0      51     129     895     954    1074    1112    1170 
   Size:         51      78     766      59     120      38      58

  Fragments arranged by size: 

                 766     120      78      59      58      51      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     596     628     718     750    1170 
   Size:        596      32      90      32     420

  Fragments arranged by size: 

                 596     420      90      32      32
 
KasI G'GCGC_C

Cuts at:      0     891    1170 
   Size:        891     279
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86    1170 
   Size:         86    1084
 
MslI CAynn'nnrTG

Cuts at:      0     301     715    1170 
   Size:        301     414     455

  Fragments arranged by size: 

                 455     414     301
 
MspA1I CmG'CkG

Cuts at:      0     252     329     939    1101    1130    1170 
   Size:        252      77     610     162      29      40

  Fragments arranged by size: 

                 610     252     162      77      40      29
 
NaeI GCC'GGC

Cuts at:      0    1088    1170 
   Size:       1088      82
 
NarI GG'CG_CC

Cuts at:      0     892    1170 
   Size:        892     278
 
NgoMIV G'CCGG_C

Cuts at:      0    1086    1170 
   Size:       1086      84
 
NspI r_CATG'y

Cuts at:      0      81    1170 
   Size:         81    1089
 
PciI A'CATG_T

Cuts at:      0      77    1170 
   Size:         77    1093
 
PfoI T'CCnGG_A

Cuts at:      0     776    1170 
   Size:        776     394
 
PpuMI rG'GwC_Cy

Cuts at:      0      99    1170 
   Size:         99    1071
 
PspXI vC'TCGA_Gb

Cuts at:      0     628     742    1048    1170 
   Size:        628     114     306     122

  Fragments arranged by size: 

                 628     306     122     114
 
PstI C_TGCA'G

Cuts at:      0     295     476     998    1135    1170 
   Size:        295     181     522     137      35

  Fragments arranged by size: 

                 522     295     181     137      35
 
SacII CC_GC'GG

Cuts at:      0    1102    1170 
   Size:       1102      68
 
SapI GCTCTTCn'nnn_

Cuts at:      0     556    1170 
   Size:        556     614
 
ScaI AGT'ACT

Cuts at:      0     289    1170 
   Size:        289     881
 
SfcI C'TryA_G

Cuts at:      0     291     472     994    1131    1170 
   Size:        291     181     522     137      39

  Fragments arranged by size: 

                 522     291     181     137      39
 
SfoI GGC'GCC

Cuts at:      0     893    1170 
   Size:        893     277
 
SmaI CCC'GGG

Cuts at:      0    1038    1170 
   Size:       1038     132
 
SmlI C'TyrA_G

Cuts at:      0     373     628     742    1048    1170 
   Size:        373     255     114     306     122

  Fragments arranged by size: 

                 373     306     255     122     114
 
StuI AGG'CCT

Cuts at:      0     706    1170 
   Size:        706     464
 
TatI w'GTAC_w

Cuts at:      0      74     287    1170 
   Size:         74     213     883

  Fragments arranged by size: 

                 883     213      74
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     772     804    1170 
   Size:        772      32     366

  Fragments arranged by size: 

                 772     366      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     808    1170 
   Size:        808     362
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     233     540    1152    1170 
   Size:        233     307     612      18

  Fragments arranged by size: 

                 612     307     233      18
 
XhoI C'TCGA_G

Cuts at:      0     628     742    1048    1170 
   Size:        628     114     306     122

  Fragments arranged by size: 

                 628     306     122     114
 
XmaI C'CCGG_G

Cuts at:      0    1036    1170 
   Size:       1036     134
 
XmnI GAAnn'nnTTC

Cuts at:      0      49    1170 
   Size:         49    1121
 
ZraI GAC'GTC

Cuts at:      0     810    1170 
   Size:        810     360
 

 Enzymes that do cut:

     AarI    AatII     AccI     AfeI   AflIII     AhdI     AleI    AlwNI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BglI
    BglII Bme1580I    BmgBI     BplI   Bpu10I    BpuEI    BsaHI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EagI     EarI    EcoNI EcoO109I    HaeII    Hin4I     KasI
     MmeI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
     PfoI    PpuMI    PspXI     PstI    SacII     SapI     ScaI     SfcI
     SfoI     SmaI     SmlI     StuI     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI    AflII     AgeI     AlfI     AloI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
    BciVI     BclI    BfrBI     BlpI     BmrI     BmtI     BpmI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiWI     BsmI    BspEI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI  Eco57MI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com