################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NdeI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 108 CGCG CG^CG AcyI 49 GGCGCC G[GA]^CG[CT]C AfaI 806 GTAC GT^AC AlwI 950 GGATC GGATC(4/5) ApaI 65 GGGCCC GGGCC^C AvaI 79 CTCGAG C^[CT]CG[GA]G BbeI 49 GGCGCC GGCGC^C BbsI 142 GAAGAC GAAGAC(2/6) BbvCI 24 GCTGAGG CCTCAGC(-5/-2) BpuAI 142 GAAGAC GAAGAC(2/6) BsaHI 49 GGCGCC G[GA]^CG[CT]C BsiEI 86 CGGCCG CG[GA][CT]^CG BsmI 522 GCATTC GAATGC(1/-1) BsoBI 79 CTCGAG C^[CT]CG[GA]G BspMI 1076 ACCTGC ACCTGC(4/8) BsrFI 88 GCCGGC [GA]^CCGG[CT] BstUI 108 CGCG CG^CG BstXI 344 CCAACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 88 GCCGGC [GA]^CCGG[CT] DraI 367 TTTAAA TTT^AAA DsaI 992 CCATGG C^C[GA][CT]GG EagI 86 CGGCCG C^GGCCG EclXI 86 CGGCCG C^GGCCG Eco52I 86 CGGCCG C^GGCCG Hin1I 49 GGCGCC G[GA]^CG[CT]C HincII 1104 GTTAAC GT[CT]^[GA]AC HindII 1104 GTTAAC GT[CT]^[GA]AC HpaI 1104 GTTAAC GTT^AAC KasI 49 GGCGCC G^GCGCC MspA1I 1099 CAGCGG C[AC]G^C[GT]G MvnI 108 CGCG CG^CG NaeI 88 GCCGGC GCC^GGC NarI 49 GGCGCC GG^CGCC NcoI 992 CCATGG C^CATGG NgoMIV 88 GCCGGC G^CCGGC PaeR7I 79 CTCGAG C^TCGAG PflMI 621 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 806 GTAC GT^AC SapI 851 GCTCTTC GCTCTTC(1/4) SfaNI 979 GATGC GCATC(5/9) SfcI 392 CTGTAG C^T[GA][CT]AG SfoI 49 GGCGCC GGC^GCC SmlI 79 CTCGAG C^T[CT][GA]AG Van91I 621 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 344 CCAACGAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 79 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 896 AAATTC [GA]^AATT[CT] 931 AAATTT ApoI 896 AAATTC [GA]^AATT[CT] 931 AAATTT AvaII 60 GGACC G^G[AT]CC 708 GGACC BcnI 63 CCGGG CC^[GC]GG 983 CCCGG BfaI 112 CTAG C^TAG 673 CTAG BglI 496 GCCTATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 820 GCCCAACAGGC BseRI 55 GAGGAG GAGGAG(10/8) 256 GAGGAG BsgI 925 GTGCAG GTGCAG(16/14) 1096 GTGCAG BspHI 237 TCATGA T^CATGA 1016 TCATGA EaeI 86 CGGCCG [CT]^GGCC[GA] 985 CGGCCA EcoT14I 468 CCTTGG C^C[AT][AT]GG 992 CCATGG FauI 68 CCCGC CCCGC(4/6) 914 GCGGG HaeII 21 GGCGCT [GA]GCGC^[CT] 49 GGCGCC HphI 42 GGTGA GGTGA(8/7) 690 GGTGA MaeI 112 CTAG C^TAG 673 CTAG NciI 63 CCGGG CC^[GC]GG 983 CCCGG NheI 111 GCTAGC G^CTAGC 672 GCTAGC RcaI 237 TCATGA T^CATGA 1016 TCATGA StyI 468 CCTTGG C^C[AT][AT]GG 992 CCATGG TaqI 80 TCGA T^CGA 866 TCGA TfiI 569 GATTC G^A[AT]TC 871 GAATC Tsp509I 897 AATT ^AATT 932 AATT TthHB8I 80 TCGA T^CGA 866 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwNI BanI BanII BbvI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI Tru9I TseI Tsp45I TspRI