Restriction map for
pFN21AE3651
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BcnI BlnI BsaBI BseAI BsiEI
BsiWI BsmFI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI HincII HindII HindIII HpaI
KpnI MamI MluI MroI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 1247 CGCG CG^CG
AclI 717 AACGTT AA^CGTT
AcyI 1403 GGCGCC G[GA]^CG[CT]C
AflII 208 CTTAAG C^TTAAG
Aor51HI 1298 AGCGCT AGC^GCT
Asp700I 1590 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1967 CCCGAG C^[CT]CG[GA]G
AviII 2510 TGCGCA TGC^GCA
BalI 1532 TGGCCA TGG^CCA
BanI 1403 GGCGCC G^G[CT][GA]CC
BanII 2479 GAGCTC G[GA]GC[CT]^C
BbeI 1403 GGCGCC GGCGC^C
BbsI 985 GAAGAC GAAGAC(2/6)
BcgI 1884 GCAGAACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1859 TGATCA T^GATCA
BfrI 208 CTTAAG C^TTAAG
BglI 2296 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1607 AGATCT A^GATCT
BlpI 317 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 317 GCTCAGC GC^T[ACGT]AGC
BpuAI 985 GAAGAC GAAGAC(2/6)
BsaAI 848 TACGTG [CT]AC^GT[GA]
BsaHI 1403 GGCGCC G[GA]^CG[CT]C
BsaI 836 GAGACC GGTCTC(1/5)
BsaWI 1286 ACCGGA [AT]^CCGG[AT]
BsmBI 1492 GAGACG CGTCTC(1/5)
BsoBI 1967 CCCGAG C^[CT]CG[GA]G
BspHI 1136 TCATGA T^CATGA
BsrBI 1738 GAGCGG CCGCTC(-3/-3)
BsrFI 839 ACCGGC [GA]^CCGG[CT]
BssSI 1870 CACGAG CACGAG(-5/-1)
BstUI 1247 CGCG CG^CG
CelII 317 GCTCAGC GC^T[ACGT]AGC
Cfr10I 839 ACCGGC [GA]^CCGG[CT]
Eco47III 1298 AGCGCT AGC^GCT
EcoNI 2556 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2141 CCATGG C^C[AT][AT]GG
EcoT22I 422 ATGCAT ATGCA^T
FbaI 1859 TGATCA T^GATCA
FspI 2510 TGCGCA TGC^GCA
HgaI 1494 GACGC GACGC(5/10)
Hin1I 1403 GGCGCC G[GA]^CG[CT]C
KasI 1403 GGCGCC G^GCGCC
KspI 1246 CCGCGG CCGC^GG
MfeI 232 CAATTG C^AATTG
MluNI 1532 TGGCCA TGG^CCA
MscI 1532 TGGCCA TGG^CCA
MunI 232 CAATTG C^AATTG
MvnI 1247 CGCG CG^CG
NarI 1403 GGCGCC GG^CGCC
NcoI 2141 CCATGG C^CATGG
NsiI 422 ATGCAT ATGCA^T
Psp1406I 717 AACGTT AA^CGTT
PstI 1577 CTGCAG CTGCA^G
RcaI 1136 TCATGA T^CATGA
SacI 2479 GAGCTC GAGCT^C
SacII 1246 CCGCGG CCGC^GG
SfoI 1403 GGCGCC GGC^GCC
StyI 2141 CCATGG C^C[AT][AT]GG
XmnI 1590 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 220 AAATTT [GA]^AATT[CT]
2655 GAATTT
AflIII 849 ACGTGT A^C[GA][CT]GT
1994 ACACGT
AlwNI 320 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1810 CAGAGACTG
ApoI 220 AAATTT [GA]^AATT[CT]
2655 GAATTT
BseRI 1173 CTCCTC GAGGAG(10/8)
2542 GAGGAG
BsgI 1990 CTGCAC GTGCAG(16/14)
2089 CTGCAC
BsmI 672 GAATGC GAATGC(1/-1)
2380 GAATGC
BsrDI 696 CATTGC GCAATG(2/0)
676 GCAATG
BstAPI 676 GCAATGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1935 GCATGCCATGC
BstXI 137 CCAGTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1514 CCATCGAGCTGG
DraIII 845 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
1870 CACGAGGTG
EaeI 841 CGGCCA [CT]^GGCC[GA]
1532 TGGCCA
EarI 1783 GAAGAG CTCTTC(1/4)
2539 GAAGAG
FauI 1219 GCGGG CCCGC(4/6)
1371 GCGGG
HaeII 1298 AGCGCT [GA]GCGC^[CT]
1403 GGCGCC
Ksp632I 1783 GAAGAG CTCTTC(1/4)
2539 GAAGAG
PpuMI 20 AGGACCC [GA]G^G[AT]CC[CT]
1915 AGGACCC
SspI 305 AATATT AAT^ATT
454 AATATT
StuI 1237 AGGCCT AGG^CCT
2387 AGGCCT
TaqI 831 TCGA T^CGA
1517 TCGA
TfiI 429 GATTC G^A[AT]TC
2214 GAATC
Tsp45I 615 GTCAC ^GT[GC]AC
969 GTCAC
TthHB8I 831 TCGA T^CGA
1517 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaII BbvI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
Eco81I EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
Tru9I TseI Tsp509I TspRI XcmI
XhoII