(Circular) (Six-base) MAPSORT of: pFN21AE3651.seq Check: 279 from: 1 to: 7399 pFN21AE3651 7399 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5209 278 Size: 53 83 186 936 3673 2468 Fragments arranged by size: 3673 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4742 1937 Size: 2805 4594 Acc65I G'GTAC_C Cuts at: 4720 4720 Size: 7399 AclI AA'CG_TT Cuts at: 1563 2723 5526 5899 1563 Size: 1160 2803 373 3063 Fragments arranged by size: 3063 2803 1160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2345 3470 3807 4547 5462 6846 7114 Size: 410 303 1125 337 740 915 1384 268 Cuts at: 7114 1632 Size: 1917 Fragments arranged by size: 1917 1384 1125 915 740 410 337 303 268 AfeI AGC'GCT Cuts at: 3305 3305 Size: 7399 AflII C'TTAA_G Cuts at: 820 1017 2213 820 Size: 197 1196 6006 Fragments arranged by size: 6006 1196 197 AflIII A'CryG_T Cuts at: 1203 2854 3999 6298 7275 1203 Size: 1651 1145 2299 977 1327 Fragments arranged by size: 2299 1651 1327 1145 977 AgeI A'CCGG_T Cuts at: 6230 6230 Size: 7399 AhdI GACnn_n'nnGTC Cuts at: 6128 6128 Size: 7399 AleI CACnn'nnGTG Cuts at: 1166 2142 1166 Size: 976 6423 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7399 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4470 4502 4470 Size: 32 7367 AlwNI CAG_nnn'CTG Cuts at: 2330 3820 5142 6714 2330 Size: 1490 1322 1572 3015 Fragments arranged by size: 3015 1572 1490 1322 ApaLI G'TGCA_C Cuts at: 5456 6612 5456 Size: 1156 6243 ApoI r'AATT_y Cuts at: 1493 2225 4660 4708 4934 5086 7195 1493 Size: 732 2435 48 226 152 2109 1697 Fragments arranged by size: 2435 2109 1697 732 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 5953 160 Size: 872 4921 1606 Fragments arranged by size: 4921 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7399 AvaI C'yCGr_G Cuts at: 1870 1943 3972 4724 1870 Size: 73 2029 752 4545 Fragments arranged by size: 4545 2029 752 73 BamHI G'GATC_C Cuts at: 4729 5096 4729 Size: 367 7032 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3408 4720 6175 618 Size: 325 217 627 1621 1312 1455 1842 Fragments arranged by size: 1842 1621 1455 1312 627 325 217 BanII G_rGCy'C Cuts at: 1391 4488 4718 1391 Size: 3097 230 4072 Fragments arranged by size: 4072 3097 230 BbeI G_GCGC'C Cuts at: 3412 3412 Size: 7399 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2997 928 Size: 971 1098 5330 Fragments arranged by size: 5330 1098 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3878 3912 5590 5624 1903 Size: 34 50 34 1857 34 1678 34 3678 Fragments arranged by size: 3678 1857 1678 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5291 6501 5291 Size: 1210 6189 BclI T'GATC_A Cuts at: 1548 3864 1548 Size: 2316 5083 BfrBI ATG'CAT Cuts at: 2429 2429 Size: 7399 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4307 6010 136 Size: 107 122 71 631 605 2635 1703 1525 Fragments arranged by size: 2635 1703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3612 7394 3612 Size: 3782 3617 BlpI GC'TnA_GC Cuts at: 2323 4816 2323 Size: 2493 4906 Bme1580I G_kGCm'C Cuts at: 1790 2546 5460 6616 1790 Size: 756 2914 1156 2573 Fragments arranged by size: 2914 2573 1156 756 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3256 5130 6088 Size: 416 53 83 399 24 1839 1874 958 Cuts at: 6088 442 Size: 1753 Fragments arranged by size: 1874 1839 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7399 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3766 4527 3766 Size: 761 6638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2547 3458 3548 4085 4394 4472 6059 1239 Size: 1308 911 90 537 309 78 1587 2579 Fragments arranged by size: 2579 1587 1308 911 537 309 90 78 Bpu10I CC'TnA_GC Cuts at: 2317 3246 2317 Size: 929 6470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3461 4199 4335 4359 4417 4550 5524 6389 6687 Size: 738 136 24 58 133 974 865 298 Cuts at: 6687 3461 Size: 4173 Fragments arranged by size: 4173 974 865 738 298 136 133 58 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2835 6062 882 Size: 617 1336 3227 2219 Fragments arranged by size: 3227 2219 1336 617 BsaAI yAC'GTr Cuts at: 493 1204 2855 5190 493 Size: 711 1651 2335 2702 Fragments arranged by size: 2702 2335 1651 711 BsaBI GATnn'nnATC Cuts at: 7393 7393 Size: 7399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 5206 5588 6223 Size: 53 83 186 936 1876 1797 382 635 Cuts at: 6223 275 Size: 1451 Fragments arranged by size: 1876 1797 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3291 5832 6230 6504 6651 7062 3291 Size: 2541 398 274 147 411 3628 Fragments arranged by size: 3628 2541 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3356 3386 4470 4500 1178 Size: 30 165 30 1953 30 1084 30 4077 Fragments arranged by size: 4077 1953 1084 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3169 4562 1186 Size: 1983 1393 4023 Fragments arranged by size: 4023 1983 1393 BseYI C'CCAG_C Cuts at: 3621 6602 7098 3621 Size: 2981 496 3922 Fragments arranged by size: 3922 2981 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3980 4079 1163 Size: 573 2244 99 4483 Fragments arranged by size: 4483 2244 573 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4849 5610 6638 1540 Size: 275 186 2848 761 1028 2301 Fragments arranged by size: 2848 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3074 3471 4254 4488 4525 5460 5545 6616 3074 Size: 397 783 234 37 935 85 1071 3857 Fragments arranged by size: 3857 1071 935 783 397 234 85 37 BsmI GAATG_Cn' Cuts at: 2683 4391 4910 5003 2683 Size: 1708 519 93 5079 Fragments arranged by size: 5079 1708 519 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3491 3491 Size: 7399 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2546 3074 3471 4254 4488 4525 4718 Size: 399 756 528 397 783 234 37 193 Cuts at: 4718 5460 5545 6616 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 756 742 528 399 397 234 193 85 37 BspEI T'CCGG_A Cuts at: 7062 7062 Size: 7399 BspHI T'CATG_A Cuts at: 3141 5288 7018 3141 Size: 2147 1730 3522 Fragments arranged by size: 3522 2147 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4754 844 Size: 3910 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3745 5286 1394 Size: 429 1922 1541 3507 Fragments arranged by size: 3507 1922 1541 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2688 2700 5894 6068 65 Size: 1183 1440 12 3194 174 1396 Fragments arranged by size: 3194 1440 1396 1183 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2844 6043 6230 1700 Size: 110 1034 3199 187 2869 Fragments arranged by size: 3199 2869 1034 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7399 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7399 BssSI C'ACGA_G Cuts at: 3875 5459 6471 3875 Size: 1584 1012 4803 Fragments arranged by size: 4803 1584 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2687 3946 794 Size: 1893 1259 4247 Fragments arranged by size: 4247 1893 1259 BstBI TT'CG_AA Cuts at: 6203 6203 Size: 7399 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7399 BstXI CCAn_nnnn'nTGG Cuts at: 2149 3526 2149 Size: 1377 6022 BstYI r'GATC_y Cuts at: 1972 2504 3345 3612 3747 4021 4729 5096 5481 Size: 532 841 267 135 274 708 367 385 Cuts at: 5481 5498 6950 7036 7048 7394 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 841 708 532 385 367 346 274 267 135 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2064 2790 4683 2064 Size: 726 1893 4780 Fragments arranged by size: 4780 1893 726 BtgI C'CryG_G Cuts at: 513 1065 3229 3251 4146 513 Size: 552 2164 22 895 3766 Fragments arranged by size: 3766 2164 895 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6660 Fragments arranged by size: 6660 683 56 BtsI GCAGTG_nn' Cuts at: 2289 3097 4915 5709 5729 2289 Size: 808 1818 794 20 3959 Fragments arranged by size: 3959 1818 808 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7399 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7364 DraI TTT'AAA Cuts at: 4703 4703 Size: 7399 DraIII CAC_nnn'GTG Cuts at: 2855 3880 2855 Size: 1025 6374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6406 809 Size: 507 5090 1802 Fragments arranged by size: 5090 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2846 3537 4846 Size: 54 1425 50 275 18 1016 691 1309 Cuts at: 4846 5735 7364 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1309 1016 889 691 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4846 1537 Size: 275 3034 4090 Fragments arranged by size: 4090 3034 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3783 4539 5329 1353 Size: 2430 756 790 3423 Fragments arranged by size: 3423 2430 790 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2663 5986 6360 6506 387 Size: 1064 138 1074 3323 374 146 1280 Fragments arranged by size: 3323 1280 1074 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4486 4486 Size: 7399 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2345 2547 3458 3470 3548 3807 Size: 393 410 303 202 911 12 78 259 Cuts at: 3807 4085 4394 4472 4547 5462 6059 6846 7114 Size: 278 309 78 75 915 597 787 268 Cuts at: 7114 1239 Size: 1524 Fragments arranged by size: 1524 915 911 787 597 410 393 309 303 278 268 259 202 78 78 75 12 EcoNI CCTnn'n_nnAGG Cuts at: 4565 4565 Size: 7399 EcoO109I rG'GnC_Cy Cuts at: 2026 3280 3921 2026 Size: 1254 641 5504 Fragments arranged by size: 5504 1254 641 EcoRI G'AATT_C Cuts at: 4708 5086 4708 Size: 378 7021 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2228 3818 2228 Size: 1590 5809 FspI TGC'GCA Cuts at: 4517 5905 4517 Size: 1388 6011 HaeII r_GCGC'y Cuts at: 1678 3307 3412 6546 1678 Size: 1629 105 3134 2531 Fragments arranged by size: 3134 2531 1629 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2489 2521 2603 Size: 32 135 32 454 32 431 32 82 Cuts at: 2603 2635 4333 4365 6040 6072 6114 6146 1373 Size: 32 1698 32 1675 32 42 32 2626 Fragments arranged by size: 2626 1698 1675 454 431 135 82 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4743 4989 669 Size: 988 281 2805 246 3079 Fragments arranged by size: 3079 2805 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7399 HpaI GTT'AAC Cuts at: 4989 4989 Size: 7399 KasI G'GCGC_C Cuts at: 3408 3408 Size: 7399 KpnI G_GTAC'C Cuts at: 4724 4724 Size: 7399 MfeI C'AATT_G Cuts at: 2237 4998 2237 Size: 2761 4638 MluI A'CGCG_T Cuts at: 7275 7275 Size: 7399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4209 6513 6697 1305 Size: 2904 2304 184 2007 Fragments arranged by size: 2904 2304 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3539 10 Size: 54 1425 343 1707 3870 Fragments arranged by size: 3870 1707 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 2424 3065 3354 4151 4479 5357 Size: 648 976 282 641 289 797 328 878 Cuts at: 5357 5716 5875 518 Size: 359 159 2042 Fragments arranged by size: 2042 976 878 797 648 641 359 328 289 282 159 MspA1I CmG'CkG Cuts at: 1606 3253 3427 3994 4063 4815 5492 6640 6885 Size: 1647 174 567 69 752 677 1148 245 Cuts at: 6885 1606 Size: 2120 Fragments arranged by size: 2120 1647 1148 752 677 567 245 174 69 NarI GG'CG_CC Cuts at: 3409 3409 Size: 7399 NcoI C'CATG_G Cuts at: 513 1065 4146 513 Size: 552 3081 3766 Fragments arranged by size: 3766 3081 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7399 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7399 NotI GC'GGCC_GC Cuts at: 4846 4846 Size: 7399 NsiI A_TGCA'T Cuts at: 2431 2431 Size: 7399 NspI r_CATG'y Cuts at: 1132 1229 3064 3106 3506 3944 4109 4757 6302 Size: 97 1835 42 400 438 165 648 1545 Cuts at: 6302 1132 Size: 2229 Fragments arranged by size: 2229 1835 1545 648 438 400 165 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7399 PciI A'CATG_T Cuts at: 6298 6298 Size: 7399 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7399 PmeI GTTT'AAAC Cuts at: 4703 4703 Size: 7399 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 4470 4502 5468 5500 6981 7013 3584 Size: 32 854 32 966 32 1481 32 3970 Fragments arranged by size: 3970 1481 966 854 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 3921 2026 Size: 1895 5504 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7399 PsiI TTA'TAA Cuts at: 3396 4969 3396 Size: 1573 5826 PstI C_TGCA'G Cuts at: 830 3586 4751 830 Size: 2756 1165 3478 Fragments arranged by size: 3478 2756 1165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7399 PvuII CAG'CTG Cuts at: 3427 3994 4063 3427 Size: 567 69 6763 Fragments arranged by size: 6763 567 69 SacI G_AGCT'C Cuts at: 4488 4488 Size: 7399 SacII CC_GC'GG Cuts at: 3254 3254 Size: 7399 SalI G'TCGA_C Cuts at: 1936 4741 1936 Size: 2805 4594 SbfI CC_TGCA'GG Cuts at: 4751 4751 Size: 7399 ScaI AGT'ACT Cuts at: 5647 7359 5647 Size: 1712 5687 SfcI C'TryA_G Cuts at: 826 1045 2173 2912 3482 3582 4403 4693 4747 Size: 219 1128 739 570 100 821 290 54 Cuts at: 4747 5882 6563 6754 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1128 821 739 681 570 290 219 191 100 54 SfoI GGC'GCC Cuts at: 3410 3410 Size: 7399 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7399 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7399 SmaI CCC'GGG Cuts at: 1872 4726 1872 Size: 2854 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 3476 4214 4350 4374 4396 Size: 197 926 270 1263 738 136 24 22 Cuts at: 4396 4529 5503 6404 6666 820 Size: 133 974 901 262 1553 Fragments arranged by size: 1553 1263 974 926 901 738 270 262 197 136 133 24 22 SnaBI TAC'GTA Cuts at: 493 5190 493 Size: 4697 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7399 SphI G_CATG'C Cuts at: 1132 3106 3506 3944 4757 1132 Size: 1974 400 438 813 3774 Fragments arranged by size: 3774 1974 813 438 400 SspI AAT'ATT Cuts at: 5 52 2312 2461 5323 5 Size: 47 2260 149 2862 2081 Fragments arranged by size: 2862 2260 2081 149 47 StuI AGG'CCT Cuts at: 1517 3244 4394 1517 Size: 1727 1150 4522 Fragments arranged by size: 4522 1727 1150 StyI C'CwwG_G Cuts at: 513 1065 4146 4838 513 Size: 552 3081 692 3074 Fragments arranged by size: 3081 3074 692 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3739 5426 5443 5596 5781 6226 325 Size: 1255 2159 1687 17 153 185 445 1498 Fragments arranged by size: 2159 1687 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 3822 3984 Size: 274 80 33 51 1443 285 1560 162 Cuts at: 3984 4068 4189 5645 7357 96 Size: 84 121 1456 1712 138 Fragments arranged by size: 1712 1560 1456 1443 285 274 162 138 121 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2841 2873 3330 3362 3950 3982 7359 7391 2841 Size: 32 457 32 588 32 3377 32 2849 Fragments arranged by size: 3377 2849 588 457 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7399 XbaI T'CTAG_A Cuts at: 4735 4735 Size: 7399 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2536 3547 2149 Size: 387 1011 6001 Fragments arranged by size: 6001 1011 387 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7399 XmaI C'CCGG_G Cuts at: 1870 4724 1870 Size: 2854 4545 XmnI GAAnn'nnTTC Cuts at: 3599 5528 3599 Size: 1929 5470 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5207 276 Size: 53 83 186 936 3673 2468 Fragments arranged by size: 3673 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI