Restriction map for
pFN21AB7034
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BspDI BsrBI
BsrDI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HphI KasI KpnI MamI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1144 GTCTAC GT^[AC][GT]AC
AccII 8 CGCG CG^CG
AccIII 277 TCCGGA T^CCGGA
AflIII 554 ACATGT A^C[GA][CT]GT
AgeI 596 ACCGGT A^CCGGT
ApaI 74 GGGCCC GGGCC^C
Asp700I 650 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 979 GGACC G^G[AT]CC
BanI 735 GGTGCC G^G[CT][GA]CC
BbsI 829 GTCTTC GAAGAC(2/6)
BclI 848 TGATCA T^GATCA
BfaI 1235 CTAG C^TAG
BpuAI 829 GTCTTC GAAGAC(2/6)
BseAI 277 TCCGGA T^CCGGA
BsgI 424 CTGCAC GTGCAG(16/14)
BsmAI 214 GTCTC GTCTC(1/5)
BsmI 256 GCATTC GAATGC(1/-1)
BspEI 277 TCCGGA T^CCGGA
BspLU11I 554 ACATGT A^CATGT
BspMI 1228 ACCTGC ACCTGC(4/8)
BstUI 8 CGCG CG^CG
BstYI 114 AGATCC [GA]^GATC[CT]
FbaI 848 TGATCA T^GATCA
HinfI 88 GAATC G^A[ACGT]TC
KspI 7 CCGCGG CCGC^GG
MaeI 1235 CTAG C^TAG
MflI 114 AGATCC [GA]^GATC[CT]
MroI 277 TCCGGA T^CCGGA
MvnI 8 CGCG CG^CG
NaeI 405 GCCGGC GCC^GGC
NgoMIV 405 GCCGGC G^CCGGC
PflMI 290 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 596 ACCGGT A^CCGGT
PstI 536 CTGCAG CTGCA^G
PvuII 944 CAGCTG CAG^CTG
SacII 7 CCGCGG CCGC^GG
SapI 893 GCTCTTC GCTCTTC(1/4)
SmlI 715 CTCAAG C^T[CT][GA]AG
TfiI 88 GAATC G^A[AT]TC
Tsp45I 532 GTCAC ^GT[GC]AC
Van91I 290 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 674 CCATCTGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 114 AGATCC [GA]^GATC[CT]
XmnI 650 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 115 GATCC GGATC(4/5)
1177 GATCC
AspHI 460 GTGCTC G[AT]GC[AT]^C
769 GTGCTC
BanII 74 GGGCCC G[GA]GC[CT]^C
742 GGGCTC
BciVI 1224 GGATAC GTATCC(6/5)
615 GTATCC
BglI 6 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
673 GCCATCTGGGC
BsiHKAI 460 GTGCTC G[AT]GC[AT]^C
769 GTGCTC
Bsp1407I 323 TGTACA T^GTACA
416 TGTACA
BspHI 575 TCATGA T^CATGA
762 TCATGA
BsrFI 405 GCCGGC [GA]^CCGG[CT]
596 ACCGGT
BsrGI 323 TGTACA T^GTACA
416 TGTACA
BstAPI 97 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1082 GCATAAGCTGC
Cfr10I 405 GCCGGC [GA]^CCGG[CT]
596 ACCGGT
EarI 236 CTCTTC CTCTTC(1/4)
894 CTCTTC
HincII 195 GTTAAC GT[CT]^[GA]AC
1269 GTTAAC
HindII 195 GTTAAC GT[CT]^[GA]AC
1269 GTTAAC
HpaI 195 GTTAAC GTT^AAC
1269 GTTAAC
Ksp632I 236 CTCTTC CTCTTC(1/4)
894 CTCTTC
MaeII 989 ACGT A^CGT
1142 ACGT
NspI 419 ACATGC [GA]CATG^[CT]
554 ACATGT
RcaI 575 TCATGA T^CATGA
762 TCATGA
SfcI 536 CTGCAG C^T[GA][CT]AG
1146 CTACAG
SspBI 323 TGTACA T^GTACA
416 TGTACA
Tsp509I 518 AATT ^AATT
1107 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
BalI BbvI BcnI BmyI BsaJI
BsaWI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI TaqI Tru9I TseI TspRI
TthHB8I XmaCI XmaI