Restriction map for
pFN21AE3668
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmI BspEI BspHI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1122 GTCTAC GT^[AC][GT]AC
AflIII 1139 ACATGT A^C[GA][CT]GT
AlwI 54 GATCC GGATC(4/5)
AvaI 331 CCCGAG C^[CT]CG[GA]G
BalI 620 TGGCCA TGG^CCA
BanI 772 GGCGCC G^G[CT][GA]CC
BanII 837 GAGCTC G[GA]GC[CT]^C
BbeI 772 GGCGCC GGCGC^C
BcgI 834 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1040 AGATCT A^GATCT
BsaAI 181 TACGTG [CT]AC^GT[GA]
BsgI 1243 CTGCAC GTGCAG(16/14)
BsiEI 1029 CGACCG CG[GA][CT]^CG
BsoBI 331 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1235 TGTACA T^GTACA
BspDI 475 ATCGAT AT^CGAT
BspLU11I 1139 ACATGT A^CATGT
BsrBI 1261 GAGCGG CCGCTC(-3/-3)
BsrFI 346 ACCGGC [GA]^CCGG[CT]
BsrGI 1235 TGTACA T^GTACA
BssHII 1128 GCGCGC G^CGCGC
BssSI 925 CTCGTG CACGAG(-5/-1)
Cfr10I 346 ACCGGC [GA]^CCGG[CT]
ClaI 475 ATCGAT AT^CGAT
DraII 83 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 87 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 83 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 503 CCAAGG C^C[AT][AT]GG
HaeII 772 GGCGCC [GA]GCGC^[CT]
KasI 772 GGCGCC G^GCGCC
KspI 126 CCGCGG CCGC^GG
MluNI 620 TGGCCA TGG^CCA
MscI 620 TGGCCA TGG^CCA
NarI 772 GGCGCC GG^CGCC
PpuMI 83 AGGACCT [GA]G^G[AT]CC[CT]
PstI 73 CTGCAG CTGCA^G
SacI 837 GAGCTC GAGCT^C
SacII 126 CCGCGG CCGC^GG
SfcI 73 CTGCAG C^T[GA][CT]AG
SfoI 772 GGCGCC GGC^GCC
SphI 1175 GCATGC GCATG^C
SspBI 1235 TGTACA T^GTACA
StyI 503 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 52 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
856 CAGCAGCTG
AocI 132 CCTGAGG CC^T[ACGT]AGG
518 CCTCAGG
BcnI 850 CCCGG CC^[GC]GG
1338 CCGGG
BpmI 139 CTGGAG CTGGAG(16/14)
811 CTGGAG
BsaI 142 GAGACC GGTCTC(1/5)
1353 GGTCTC
BspMI 938 ACCTGC ACCTGC(4/8)
1008 GCAGGT
BsrDI 601 GCAATG GCAATG(2/0)
689 GCAATG
BsrI 497 ACTGG ACTGG(1/-1)
158 CCAGT
BstAPI 680 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
689 GCAATGCCTGC
BstYI 53 AGATCC [GA]^GATC[CT]
1040 AGATCT
Bsu36I 132 CCTGAGG CC^T[ACGT]AGG
518 CCTCAGG
DsaI 126 CCGCGG C^C[GA][CT]GG
948 CCACGG
EaeI 620 TGGCCA [CT]^GGCC[GA]
1335 TGGCCG
Eco81I 132 CCTGAGG CC^T[ACGT]AGG
518 CCTCAGG
FauI 1150 GCGGG CCCGC(4/6)
1159 GCGGG
HinfI 784 GACTC G^A[ACGT]TC
1318 GAGTC
MaeII 182 ACGT A^CGT
1199 ACGT
MflI 53 AGATCC [GA]^GATC[CT]
1040 AGATCT
NciI 850 CCCGG CC^[GC]GG
1338 CCGGG
PleI 784 GACTC GAGTC(4/5)
1318 GAGTC
SapI 363 GCTCTTC GCTCTTC(1/4)
942 GCTCTTC
XhoII 53 AGATCC [GA]^GATC[CT]
1040 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII AviII BbsI BbvI
BmyI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
Ksp632I MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StuI TaqI TseI TspRI TthHB8I