################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AviII BanI BbeI BbrPI BbvCI BciVI BclI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AciI 1222 GCGG CCGC(-3/-1) AflII 637 CTTAAG C^TTAAG Alw44I 1176 GTGCAC G^TGCAC AlwNI 1285 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1176 GTGCAC G^TGCAC AseI 333 ATTAAT AT^TAAT AsnI 333 ATTAAT AT^TAAT Asp700I 384 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 898 CCCGAG C^[CT]CG[GA]G AvrII 750 CCTAGG C^CTAGG BalI 879 TGGCCA TGG^CCA BamHI 585 GGATCC G^GATCC BanII 988 GGGCTC G[GA]GC[CT]^C BbsI 492 GAAGAC GAAGAC(2/6) BcgI 1168 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 75 CCGGG CC^[GC]GG BfrI 637 CTTAAG C^TTAAG BglI 941 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 750 CCTAGG C^CTAGG BpuAI 492 GAAGAC GAAGAC(2/6) BsaI 238 GAGACC GGTCTC(1/5) BseRI 944 GAGGAG GAGGAG(10/8) BsoBI 898 CCCGAG C^[CT]CG[GA]G BspDI 769 ATCGAT AT^CGAT BspHI 344 TCATGA T^CATGA BsrDI 782 GCAATG GCAATG(2/0) BstXI 870 CCAGATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 653 GCGC GCG^C ClaI 769 ATCGAT AT^CGAT DraII 113 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 879 TGGCCA [CT]^GGCC[GA] EcoO109I 113 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1150 GAATTC G^AATTC EcoT22I 856 ATGCAT ATGCA^T HaeII 652 GGCGCT [GA]GCGC^[CT] HapII 75 CCGG C^CGG HhaI 653 GCGC GCG^C HinP1I 653 GCGC G^CGC HindIII 695 AAGCTT A^AGCTT HpaII 75 CCGG C^CGG MaeII 207 ACGT A^CGT MaeIII 542 GTTAC ^GT[ACGT]AC MluNI 879 TGGCCA TGG^CCA MscI 879 TGGCCA TGG^CCA MspA1I 1096 CAGCTG C[AC]G^C[GT]G MspI 75 CCGG C^CGG NciI 75 CCGGG CC^[GC]GG NsiI 856 ATGCAT ATGCA^T PpuMI 113 GGGACCT [GA]G^G[AT]CC[CT] PshBI 333 ATTAAT AT^TAAT PvuII 1096 CAGCTG CAG^CTG RcaI 344 TCATGA T^CATGA SapI 738 GAAGAGC GCTCTTC(1/4) SspI 1385 AATATT AAT^ATT XcmI 954 CCAGAGTGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 384 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 838 AAATTC [GA]^AATT[CT] 1150 GAATTC AflIII 1226 ACATGT A^C[GA][CT]GT 1346 ACATGT ApoI 838 AAATTC [GA]^AATT[CT] 1150 GAATTC AspHI 901 GAGCAC G[AT]GC[AT]^C 1176 GTGCAC AvaII 114 GGACC G^G[AT]CC 396 GGACC BfaI 751 CTAG C^TAG 1042 CTAG BsiHKAI 901 GAGCAC G[AT]GC[AT]^C 1176 GTGCAC BspLU11I 1226 ACATGT A^CATGT 1346 ACATGT BsrI 444 ACTGG ACTGG(1/-1) 667 CCAGT BstYI 585 GGATCC [GA]^GATC[CT] 1320 GGATCT EcoNI 342 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1113 CCTCTTGGAGG EcoT14I 750 CCTAGG C^C[AT][AT]GG 1376 CCAAGG MaeI 751 CTAG C^TAG 1042 CTAG MflI 585 GGATCC [GA]^GATC[CT] 1320 GGATCT MseI 334 TTAA T^TAA 638 TTAA NspI 1226 ACATGT [GA]CATG^[CT] 1346 ACATGT PleI 978 GACTC GAGTC(4/5) 576 GAGTC PstI 1270 CTGCAG CTGCA^G 1399 CTGCAG SmlI 580 CTCAAG C^T[CT][GA]AG 637 CTTAAG StyI 750 CCTAGG C^C[AT][AT]GG 1376 CCAAGG TaqI 770 TCGA T^CGA 801 TCGA Tru9I 334 TTAA T^TAA 638 TTAA TspRI 668 CAGTG CA[GC]TG[ACGT][ACGT]^ 1273 CAGTG TthHB8I 770 TCGA T^CGA 801 TCGA XhoII 585 GGATCC [GA]^GATC[CT] 1320 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AfaI AluI AlwI BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp509I