(Circular) (Six-base) MAPSORT of: pFN21AE1312.seq Check: 3563 from: 1 to: 6145 pFN21AE1312 6145 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2259 2259 Size: 6145 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 2516 2590 2745 4208 5592 5860 1632 Size: 402 482 74 155 1463 1384 268 1917 Fragments arranged by size: 1917 1463 1384 482 402 268 155 74 AflII C'TTAA_G Cuts at: 820 1017 2642 820 Size: 197 1625 4323 Fragments arranged by size: 4323 1625 197 AflIII A'CryG_T Cuts at: 1203 3231 3351 5044 6021 1203 Size: 2028 120 1693 977 1327 Fragments arranged by size: 2028 1693 1327 977 120 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 3295 3888 5460 3295 Size: 593 1572 3980 Fragments arranged by size: 3980 1572 593 ApaLI G'TGCA_C Cuts at: 3181 4202 5358 3181 Size: 1021 1156 3968 Fragments arranged by size: 3968 1156 1021 ApoI r'AATT_y Cuts at: 1493 2843 3155 3454 3680 3832 5941 1493 Size: 1350 312 299 226 152 2109 1697 Fragments arranged by size: 2109 1697 1350 312 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 2339 4699 160 Size: 872 1307 2360 1606 Fragments arranged by size: 2360 1606 1307 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2903 3470 1870 Size: 73 960 567 4545 Fragments arranged by size: 4545 960 567 73 AvrII C'CTAG_G Cuts at: 2755 2755 Size: 6145 BamHI G'GATC_C Cuts at: 2590 3475 3842 2590 Size: 885 367 4893 Fragments arranged by size: 4893 885 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3466 4921 618 Size: 325 217 627 1679 1455 1842 Fragments arranged by size: 1842 1679 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2997 3464 1391 Size: 1606 467 4072 Fragments arranged by size: 4072 1606 467 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2504 928 Size: 971 605 4569 Fragments arranged by size: 4569 971 605 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3162 3196 4336 4370 1903 Size: 34 1225 34 1140 34 3678 Fragments arranged by size: 3678 1225 1140 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BfrBI ATG'CAT Cuts at: 2863 2863 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2952 4756 136 Size: 107 122 71 631 605 1280 1804 1525 Fragments arranged by size: 1804 1525 1280 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 3185 4206 5362 1790 Size: 1395 1021 1156 2573 Fragments arranged by size: 2573 1395 1156 1021 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3125 3232 3263 4805 1239 Size: 1886 107 31 1542 2579 Fragments arranged by size: 2579 1886 1542 107 31 Bpu10I CC'TnA_GC Cuts at: 2255 2255 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 4270 5135 5433 2570 Size: 1700 865 298 3282 Fragments arranged by size: 3282 1700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 4808 882 Size: 617 738 2571 2219 Fragments arranged by size: 2571 2219 738 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3952 4334 4969 275 Size: 53 83 186 936 2419 382 635 1451 Fragments arranged by size: 2419 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3205 3235 3303 3333 1178 Size: 30 165 30 1802 30 68 30 3990 Fragments arranged by size: 3990 1802 165 68 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2964 1186 Size: 1778 4367 BseYI C'CCAG_C Cuts at: 5348 5844 5348 Size: 496 5649 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5572 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2910 3185 4206 4291 5362 2910 Size: 275 1021 85 1071 3693 Fragments arranged by size: 3693 1071 1021 275 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2910 2997 3185 3464 4206 4291 5362 Size: 399 1120 87 188 279 742 85 1071 Cuts at: 5362 1391 Size: 2174 Fragments arranged by size: 2174 1120 1071 742 399 279 188 87 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 2349 4034 5764 2349 Size: 1685 1730 2730 Fragments arranged by size: 2730 1730 1685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 2259 3282 3500 844 Size: 1367 48 1023 218 3489 Fragments arranged by size: 3489 1367 1023 218 48 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2794 4640 4814 65 Size: 1183 1546 1846 174 1396 Fragments arranged by size: 1846 1546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4789 4976 1700 Size: 110 2979 187 2869 Fragments arranged by size: 2979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstXI CCAn_nnnn'nTGG Cuts at: 2882 2882 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2590 3325 3475 3842 4227 4244 5696 5782 Size: 618 735 150 367 385 17 1452 86 Cuts at: 5782 5794 6140 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 735 618 385 367 346 150 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5593 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3155 487 Size: 683 56 1929 3477 Fragments arranged by size: 3477 1929 683 56 BtsI GCAGTG_nn' Cuts at: 3284 3661 4455 4475 3284 Size: 377 794 20 4954 Fragments arranged by size: 4954 794 377 20 ClaI AT'CG_AT Cuts at: 1554 2775 1554 Size: 1221 4924 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 3592 4481 Size: 54 1425 50 275 18 1054 708 889 Cuts at: 4481 6110 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1054 889 708 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 2721 2738 4075 1353 Size: 1031 337 17 1337 3423 Fragments arranged by size: 3423 1337 1031 337 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2516 2590 2745 3125 3232 3263 Size: 393 402 482 74 155 380 107 31 Cuts at: 3263 4208 4805 5592 5860 1239 Size: 945 597 787 268 1524 Fragments arranged by size: 1524 945 787 597 482 402 393 380 268 155 107 74 31 EcoNI CCTnn'n_nnAGG Cuts at: 2351 3122 2351 Size: 771 5374 EcoO109I rG'GnC_Cy Cuts at: 2119 2119 Size: 6145 EcoRI G'AATT_C Cuts at: 3155 3454 3832 3155 Size: 299 378 5468 Fragments arranged by size: 5468 378 299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2057 2413 2057 Size: 356 5789 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2661 5292 1678 Size: 983 2631 2531 Fragments arranged by size: 2631 2531 983 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 4786 4818 4860 Size: 32 135 32 746 32 2436 32 42 Cuts at: 4860 4892 1373 Size: 32 2626 Fragments arranged by size: 2626 2436 746 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 2700 748 Size: 1952 4193 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 5259 5443 1305 Size: 1092 2862 184 2007 Fragments arranged by size: 2862 2007 1092 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2886 10 Size: 54 1425 343 1054 3269 Fragments arranged by size: 3269 1425 1054 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4103 4462 4621 518 Size: 648 2937 359 159 2042 Fragments arranged by size: 2937 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3103 3561 4238 5386 5631 1606 Size: 1497 458 677 1148 245 2120 Fragments arranged by size: 2120 1497 1148 677 458 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5593 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NsiI A_TGCA'T Cuts at: 2865 2865 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3235 3355 3503 5048 1132 Size: 97 2006 120 148 1545 2229 Fragments arranged by size: 2229 2006 1545 148 120 97 PasI CC'CwG_GG Cuts at: 1348 2824 1348 Size: 1476 4669 PciI A'CATG_T Cuts at: 3231 3351 5044 3231 Size: 120 1693 4332 Fragments arranged by size: 4332 1693 120 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PfoI T'CCnGG_A Cuts at: 3321 3321 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2119 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PstI C_TGCA'G Cuts at: 830 3279 3408 3497 830 Size: 2449 129 89 3478 Fragments arranged by size: 3478 2449 129 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 3103 3103 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SapI GCTCTTCn'nnn_ Cuts at: 2738 2738 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2091 2182 2281 3275 3404 3493 4628 Size: 219 1046 91 99 994 129 89 1135 Cuts at: 4628 5309 5500 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1046 994 681 219 191 129 99 91 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 2642 4249 5150 5412 820 Size: 197 926 642 57 1607 901 262 1553 Fragments arranged by size: 1607 1553 926 901 642 262 197 57 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 3392 4069 5 Size: 47 3340 677 2081 Fragments arranged by size: 3340 2081 677 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2755 3381 3584 513 Size: 552 1690 626 203 3074 Fragments arranged by size: 3074 1690 626 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4172 4189 4342 4527 4972 325 Size: 1255 2592 17 153 185 445 1498 Fragments arranged by size: 2592 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3221 4391 Size: 274 80 33 51 1443 537 707 1170 Cuts at: 4391 6103 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1170 707 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6105 6137 6105 Size: 32 6113 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2966 2966 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 2393 4274 2393 Size: 1881 4264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI