################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BcgI BfaI BfrI BglII BlnI BpmI BpuAI BsaBI BsaI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT14I EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 205 GTCTAC GT^[AC][GT]AC AccIII 542 TCCGGA T^CCGGA AcsI 462 GAATTC [GA]^AATT[CT] AcyI 117 GACGCC G[GA]^CG[CT]C AfaI 579 GTAC GT^AC AflIII 575 ACACGT A^C[GA][CT]GT Alw44I 841 GTGCAC G^TGCAC AlwNI 710 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 24 GGGCCC GGGCC^C ApaLI 841 GTGCAC G^TGCAC ApoI 462 GAATTC [GA]^AATT[CT] AspHI 841 GTGCAC G[AT]GC[AT]^C BamHI 232 GGATCC G^GATCC BbvCI 490 CCTCAGC CCTCAGC(-5/-2) BciVI 936 GGATAC GTATCC(6/5) BclI 615 TGATCA T^GATCA BglI 974 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 908 GCTCAGC GC^T[ACGT]AGC Bpu1102I 908 GCTCAGC GC^T[ACGT]AGC BsaAI 576 CACGTA [CT]AC^GT[GA] BsaHI 117 GACGCC G[GA]^CG[CT]C BsaWI 542 TCCGGA [AT]^CCGG[AT] BseAI 542 TCCGGA T^CCGGA BsgI 501 CTGCAC GTGCAG(16/14) BsiHKAI 841 GTGCAC G[AT]GC[AT]^C BsmAI 1047 GAGAC GTCTC(1/5) BspEI 542 TCCGGA T^CCGGA BspMI 662 ACCTGC ACCTGC(4/8) BsrBI 80 GAGCGG CCGCTC(-3/-3) BsrDI 760 GCAATG GCAATG(2/0) BstAPI 63 GCATGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 878 GGTCACC G^GT[ACGT]ACC BstPI 878 GGTCACC G^GT[ACGT]ACC BstXI 755 CCAATGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 232 GGATCC [GA]^GATC[CT] CelII 908 GCTCAGC GC^T[ACGT]AGC CfoI 666 GCGC GCG^C DsaI 395 CCGTGG C^C[GA][CT]GG EcoO65I 878 GGTCACC G^GT[ACGT]ACC EcoRI 462 GAATTC G^AATTC EcoRV 646 GATATC GAT^ATC FbaI 615 TGATCA T^GATCA HgaI 117 GACGC GACGC(5/10) HhaI 666 GCGC GCG^C Hin1I 117 GACGCC G[GA]^CG[CT]C HinP1I 666 GCGC G^CGC HindIII 529 AAGCTT A^AGCTT MaeII 577 ACGT A^CGT MboII 546 GAAGA GAAGA(8/7) MflI 232 GGATCC [GA]^GATC[CT] MroI 542 TCCGGA T^CCGGA MslI 1006 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PstI 248 CTGCAG CTGCA^G PvuII 902 CAGCTG CAG^CTG RsaI 579 GTAC GT^AC SexAI 581 ACCAGGT A^CC[AT]GGT SfcI 248 CTGCAG C^T[GA][CT]AG SspI 954 AATATT AAT^ATT TaqI 1036 TCGA T^CGA TthHB8I 1036 TCGA T^CGA XcmI 986 CCATCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 232 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 233 GATCC GGATC(4/5) 232 GGATC AvaII 142 GGACC G^G[AT]CC 660 GGACC BanII 24 GGGCCC G[GA]GC[CT]^C 1015 GAGCCC BsaJI 93 CCCAGG C^C[ACGT][ACGT]GG 395 CCGTGG BsiYI 94 CCAGGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 941 CCACAAGCAGG BslI 94 CCAGGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 941 CCACAAGCAGG DpnII 233 GATC ^GATC 616 GATC EaeI 793 CGGCCA [CT]^GGCC[GA] 972 CGGCCA FauI 82 GCGGG CCCGC(4/6) 139 GCGGG HinfI 706 GATTC G^A[ACGT]TC 1065 GATTC MboI 233 GATC ^GATC 616 GATC NdeII 233 GATC ^GATC 616 GATC PpuMI 141 GGGACCT [GA]G^G[AT]CC[CT] 659 GGGACCT Sau3AI 233 GATC ^GATC 616 GATC TfiI 706 GATTC G^A[AT]TC 1065 GATTC Tsp509I 341 AATT ^AATT 463 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI BbvI BcnI BmyI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI