(Circular) (Six-base) MAPSORT of: pFN21AE5406.seq Check: 9198 from: 1 to: 5776 pFN21AE5406 5776 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2211 3119 1937 Size: 274 908 4594 Fragments arranged by size: 4594 908 274 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2760 3839 5223 5491 1632 Size: 707 421 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1079 707 421 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2580 4675 5652 1203 Size: 1377 2095 977 1327 Fragments arranged by size: 2095 1377 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2720 3519 5091 2720 Size: 799 1572 3405 Fragments arranged by size: 3405 1572 799 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 5776 ApaLI G'TGCA_C Cuts at: 2846 3833 4989 2846 Size: 987 1156 3633 Fragments arranged by size: 3633 1156 987 ApoI r'AATT_y Cuts at: 1493 2467 3085 3311 3463 5572 1493 Size: 974 618 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 618 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 2237 3106 3473 2237 Size: 869 367 4540 Fragments arranged by size: 4540 869 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2033 3024 3095 1391 Size: 642 991 71 4072 Fragments arranged by size: 4072 991 642 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BbvCI CC'TCA_GC Cuts at: 2496 2496 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2935 3668 4878 2935 Size: 733 1210 3833 Fragments arranged by size: 3833 1210 733 BclI T'GATC_A Cuts at: 1548 2620 1548 Size: 1072 4704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2985 4387 136 Size: 107 122 71 631 605 1313 1402 1525 Fragments arranged by size: 1525 1402 1313 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 2914 3193 2914 Size: 279 5497 Bme1580I G_kGCm'C Cuts at: 1790 2033 2850 3837 4993 1790 Size: 243 817 987 1156 2573 Fragments arranged by size: 2573 1156 987 817 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2511 2511 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4436 1239 Size: 3197 2579 Bpu10I CC'TnA_GC Cuts at: 2245 2496 2245 Size: 251 5525 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2583 3567 493 Size: 711 1379 984 2702 Fragments arranged by size: 2702 1379 984 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 3583 3965 4600 Size: 53 83 186 936 590 1460 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1460 1451 936 635 590 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2547 4209 4607 4881 5028 5439 2547 Size: 1662 398 274 147 411 2884 Fragments arranged by size: 2884 1662 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 4979 5475 4979 Size: 496 5280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2491 1163 Size: 573 755 4448 Fragments arranged by size: 4448 755 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2850 3837 3922 4993 2850 Size: 987 85 1071 3633 Fragments arranged by size: 3633 1071 987 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2850 3024 3095 3837 3922 4993 Size: 399 243 817 174 71 742 85 1071 Cuts at: 4993 1391 Size: 2174 Fragments arranged by size: 2174 1071 817 742 399 243 174 85 71 BspEI T'CCGG_A Cuts at: 2547 5439 2547 Size: 2892 2884 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 3131 844 Size: 1832 455 3489 Fragments arranged by size: 3489 1832 455 BsrBI CCG'CTC Cuts at: 1394 1823 2087 3663 1394 Size: 429 264 1576 3507 Fragments arranged by size: 3507 1576 429 264 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 4271 4445 65 Size: 1183 1524 1499 174 1396 Fragments arranged by size: 1524 1499 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 794 Size: 1280 4496 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 2883 1184 Size: 1699 4077 BstXI CCAn_nnnn'nTGG Cuts at: 2767 2767 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2237 3106 3473 3858 3875 5327 5413 5425 Size: 265 869 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 869 385 367 346 265 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2400 513 Size: 552 1335 3889 Fragments arranged by size: 3889 1335 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2343 2521 3292 4086 4106 2343 Size: 178 771 794 20 4013 Fragments arranged by size: 4013 794 771 178 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2798 2977 3223 Size: 54 1425 50 275 18 968 179 246 Cuts at: 3223 4112 5741 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 968 889 275 246 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2760 3839 4436 5223 5491 1239 Size: 393 707 421 1079 597 787 268 1524 Fragments arranged by size: 1524 1079 787 707 597 421 393 268 EcoO109I rG'GnC_Cy Cuts at: 2029 2147 2492 2665 2029 Size: 118 345 173 5140 Fragments arranged by size: 5140 345 173 118 EcoRI G'AATT_C Cuts at: 2467 3085 3463 2467 Size: 618 378 4780 Fragments arranged by size: 4780 618 378 EcoRV GAT'ATC Cuts at: 2653 2653 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 2534 748 Size: 1786 3990 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3015 3734 4093 4252 518 Size: 648 1849 719 359 159 2042 Fragments arranged by size: 2042 1849 719 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2065 2909 3192 3869 5017 5262 1606 Size: 438 21 844 283 677 1148 245 2120 Fragments arranged by size: 2120 1148 844 677 438 283 245 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2719 2751 3845 3877 5358 5390 2719 Size: 32 1094 32 1481 32 3105 Fragments arranged by size: 3105 1481 1094 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2147 2665 2147 Size: 518 5258 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 5776 PstI C_TGCA'G Cuts at: 830 2257 3128 830 Size: 1427 871 3478 Fragments arranged by size: 3478 1427 871 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2909 2909 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2253 3124 4259 4940 5131 826 Size: 219 1208 871 1135 681 191 1471 Fragments arranged by size: 1471 1208 1135 871 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 2961 3700 5 Size: 47 2909 739 2081 Fragments arranged by size: 2909 2081 739 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 3215 513 Size: 552 2150 3074 Fragments arranged by size: 3074 2150 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2280 3803 3820 3973 4158 4603 325 Size: 1255 700 1523 17 153 185 445 1498 Fragments arranged by size: 1523 1498 1255 700 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2998 2998 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI