Restriction map for pFN21AE1566
 (Linear) (Six-base) MAPSORT of: KIEE1566.seq  Check: 2938  from: 1  to: 1425



KIEE1566.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

Acc65I G'GTAC_C

Cuts at:      0     606    1425 
   Size:        606     819
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     931    1229    1425 
   Size:        931     298     196

  Fragments arranged by size: 

                 931     298     196
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     225     679    1425 
   Size:         18     207     454     746

  Fragments arranged by size: 

                 746     454     207      18
 
ApaI G_GGCC'C

Cuts at:      0      54    1425 
   Size:         54    1371
 
AvaI C'yCGr_G

Cuts at:      0      36    1425 
   Size:         36    1389
 
BanI G'GyrC_C

Cuts at:      0     606    1425 
   Size:        606     819
 
BanII G_rGCy'C

Cuts at:      0      54     138     664     901    1230    1425 
   Size:         54      84     526     237     329     195

  Fragments arranged by size: 

                 526     329     237     195      84      54
 
BfrBI ATG'CAT

Cuts at:      0    1413    1425 
   Size:       1413      12
 
Bme1580I G_kGCm'C

Cuts at:      0      54    1425 
   Size:         54    1371
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     812    1385    1425 
   Size:        812     573      40

  Fragments arranged by size: 

                 812     573      40
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     584     604    1136    1425 
   Size:        362     222      20     532     289

  Fragments arranged by size: 

                 532     362     289     222      20
 
Bpu10I CC'TnA_GC

Cuts at:      0     171    1425 
   Size:        171    1254
 
BsaAI yAC'GTr

Cuts at:      0     621    1425 
   Size:        621     804
 
BsaHI Gr'CG_yC

Cuts at:      0     915    1425 
   Size:        915     510
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     446     476     492     522     738     768     795     825 
   Size:        446      30      16      30     216      30      27      30

Cuts at:    825    1077    1107    1425 
   Size:        252      30     318

  Fragments arranged by size: 

                 446     318     252     216      30      30      30      30
                  30      27      16
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     803     992     995     998    1022    1133    1136    1139 
   Size:        803     189       3       3      24     111       3       3

Cuts at:   1139    1183    1425 
   Size:         44     242

  Fragments arranged by size: 

                 803     242     189     111      44      24       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     242    1425 
   Size:        242    1183
 
Bsp1286I G_dGCh'C

Cuts at:      0      54     138     664     901    1230    1425 
   Size:         54      84     526     237     329     195

  Fragments arranged by size: 

                 526     329     237     195      84      54
 
BsrGI T'GTAC_A

Cuts at:      0     840    1425 
   Size:        840     585
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      18     181    1425 
   Size:         18     163    1244

  Fragments arranged by size: 

                1244     163      18
 
BtgI C'CryG_G

Cuts at:      0    1222    1425 
   Size:       1222     203
 
BtsI GCAGTG_nn'

Cuts at:      0     717    1425 
   Size:        717     708
 
EaeI y'GGCC_r

Cuts at:      0      86     203    1058    1425 
   Size:         86     117     855     367

  Fragments arranged by size: 

                 855     367     117      86
 
EarI CTCTTCn'nnn_

Cuts at:      0      20      61     312    1160    1205    1425 
   Size:         20      41     251     848      45     220

  Fragments arranged by size: 

                 848     251     220      45      41      20
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1425 
   Size:         19    1406
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     584     604     931    1136    1229    1425 
   Size:        362     222      20     327     205      93     196

  Fragments arranged by size: 

                 362     327     222     205     196      93      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     539    1425 
   Size:        539     886
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     967    1215    1425 
   Size:         51     916     248     210

  Fragments arranged by size: 

                 916     248     210      51
 
EcoRV GAT'ATC

Cuts at:      0     231    1425 
   Size:        231    1194
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     571    1425 
   Size:        571     854
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     360     392     738     770    1279    1311    1425 
   Size:        360      32     346      32     509      32     114

  Fragments arranged by size: 

                 509     360     346     114      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     610    1425 
   Size:        610     815
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     724    1425 
   Size:        535     189     701

  Fragments arranged by size: 

                 701     535     189
 
MscI TGG'CCA

Cuts at:      0     205    1060    1425 
   Size:        205     855     365

  Fragments arranged by size: 

                 855     365     205
 
MslI CAynn'nnrTG

Cuts at:      0     481    1425 
   Size:        481     944
 
NsiI A_TGCA'T

Cuts at:      0    1415    1425 
   Size:       1415      10
 
PpuMI rG'GwC_Cy

Cuts at:      0     967    1215    1425 
   Size:        967     248     210

  Fragments arranged by size: 

                 967     248     210
 
PspOMI G'GGCC_C

Cuts at:      0      50    1425 
   Size:         50    1375
 
PstI C_TGCA'G

Cuts at:      0      14     221    1425 
   Size:         14     207    1204

  Fragments arranged by size: 

                1204     207      14
 
SfcI C'TryA_G

Cuts at:      0      10     217     849     909    1021    1425 
   Size:         10     207     632      60     112     404

  Fragments arranged by size: 

                 632     404     207     112      60      10
 
SmaI CCC'GGG

Cuts at:      0      38    1425 
   Size:         38    1387
 
SnaBI TAC'GTA

Cuts at:      0     621    1425 
   Size:        621     804
 
SspI AAT'ATT

Cuts at:      0    1077    1425 
   Size:       1077     348
 
StuI AGG'CCT

Cuts at:      0     864    1425 
   Size:        864     561
 
StyI C'CwwG_G

Cuts at:      0     200    1425 
   Size:        200    1225
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     178     475     749    1425 
   Size:        178     297     274     676

  Fragments arranged by size: 

                 676     297     274     178
 
TatI w'GTAC_w

Cuts at:      0     840    1425 
   Size:        840     585
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     446     478    1207    1239    1425 
   Size:        446      32     729      32     186

  Fragments arranged by size: 

                 729     446     186      32      32
 
XbaI T'CTAG_A

Cuts at:      0     435    1425 
   Size:        435     990
 
XmaI C'CCGG_G

Cuts at:      0      36    1425 
   Size:         36    1389
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApaI     AvaI     BanI    BanII    BfrBI
 Bme1580I     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI
    BseYI Bsp1286I    BsrGI   BstAPI     BtgI     BtsI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    Hin4I     KpnI
     MmeI     MscI     MslI     NsiI    PpuMI   PspOMI     PstI     SfcI
     SmaI    SnaBI     SspI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaBI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI     SpeI     SphI     SrfI     SwaI  Tth111I     XcmI     XhoI
     XmnI     ZraI
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