################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BbrPI BbvCI BcgI BclI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HinfI HpaI KspI MaeII MamI MluI MroI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI TfiI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1156 GGTACC G^GTACC AcyI 1017 GGCGCC G[GA]^CG[CT]C AflII 1389 CTTAAG C^TTAAG Asp718I 1156 GGTACC G^GTACC AspHI 1110 GAGCAC G[AT]GC[AT]^C BalI 972 TGGCCA TGG^CCA BamHI 577 GGATCC G^GATCC BbeI 1017 GGCGCC GGCGC^C BbsI 459 GAAGAC GAAGAC(2/6) BfrI 1389 CTTAAG C^TTAAG BglII 1580 AGATCT A^GATCT BpuAI 459 GAAGAC GAAGAC(2/6) BsaHI 1017 GGCGCC G[GA]^CG[CT]C BsaWI 1510 TCCGGT [AT]^CCGG[AT] BseRI 1963 CTCCTC GAGGAG(10/8) BsiHKAI 1110 GAGCAC G[AT]GC[AT]^C BspDI 1433 ATCGAT AT^CGAT BsrDI 529 GCAATG GCAATG(2/0) BstXI 1721 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1433 ATCGAT AT^CGAT DsaI 548 CCATGG C^C[GA][CT]GG EaeI 972 TGGCCA [CT]^GGCC[GA] EcoT22I 1523 ATGCAT ATGCA^T HaeII 1017 GGCGCC [GA]GCGC^[CT] HgaI 1914 GCGTC GACGC(5/10) Hin1I 1017 GGCGCC G[GA]^CG[CT]C HindIII 441 AAGCTT A^AGCTT KasI 1017 GGCGCC G^GCGCC KpnI 1156 GGTACC GGTAC^C MfeI 1217 CAATTG C^AATTG MluNI 972 TGGCCA TGG^CCA MscI 972 TGGCCA TGG^CCA MseI 1390 TTAA T^TAA MunI 1217 CAATTG C^AATTG NarI 1017 GGCGCC GG^CGCC NcoI 548 CCATGG C^CATGG NsiI 1523 ATGCAT ATGCA^T SfoI 1017 GGCGCC GGC^GCC StuI 1773 AGGCCT AGG^CCT TaqI 1434 TCGA T^CGA Tru9I 1390 TTAA T^TAA Tsp509I 1218 AATT ^AATT TthHB8I 1434 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### ApaI 492 GGGCCC GGGCC^C 1362 GGGCCC AspI 1462 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1536 GACTGTGTC AvaI 812 CTCGGG C^[CT]CG[GA]G 1683 CTCGGG BciVI 144 GTATCC GTATCC(6/5) 363 GTATCC BcnI 573 CCGGG CC^[GC]GG 581 CCCGG BglI 291 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 413 GCCCATCTGGC BlpI 507 GCTGAGC GC^T[ACGT]AGC 611 GCTCAGC Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC 611 GCTCAGC BsaI 1273 GAGACC GGTCTC(1/5) 428 GGTCTC BsoBI 812 CTCGGG C^[CT]CG[GA]G 1683 CTCGGG BstAPI 1497 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1925 GCATCTTCTGC CelII 507 GCTGAGC GC^T[ACGT]AGC 611 GCTCAGC CfoI 1018 GCGC GCG^C 1851 GCGC EarI 435 GAAGAG CTCTTC(1/4) 1401 GAAGAG EcoNI 128 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1776 CCTTGGGCAGG HhaI 1018 GCGC GCG^C 1851 GCGC HinP1I 1018 GCGC G^CGC 1851 GCGC HincII 313 GTCAAC GT[CT]^[GA]AC 1176 GTCAAC HindII 313 GTCAAC GT[CT]^[GA]AC 1176 GTCAAC Ksp632I 435 GAAGAG CTCTTC(1/4) 1401 GAAGAG NciI 573 CCGGG CC^[GC]GG 581 CCCGG PflFI 1462 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1536 GACTGTGTC PflMI 122 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 496 CCAGTGTCTGG SbfI 873 CCTGCAGG CCTGCA^GG 1494 CCTGCAGG SexAI 632 ACCAGGT A^CC[AT]GGT 1008 ACCTGGT Sse8387I 873 CCTGCAGG CCTGCA^GG 1494 CCTGCAGG Tth111I 1462 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1536 GACTGTGTC Van91I 122 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 496 CCAGTGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI Tsp45I TspRI XcmI XhoII