(Circular) (Six-base) MAPSORT of: pFN21AE4551.seq Check: 1127 from: 1 to: 6688 pFN21AE4551 6688 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3049 3217 3049 Size: 168 6520 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4498 278 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2568 3341 3922 4031 1937 Size: 631 773 581 109 4594 Fragments arranged by size: 4594 773 631 581 109 Acc65I G'GTAC_C Cuts at: 3161 4009 3161 Size: 848 5840 AclI AA'CG_TT Cuts at: 1563 4815 5188 1563 Size: 3252 373 3063 Fragments arranged by size: 3252 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2781 3093 3333 3698 4751 6135 6403 1632 Size: 1149 312 240 365 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1149 1053 365 312 268 240 AflII C'TTAA_G Cuts at: 820 1017 3394 820 Size: 197 2377 4114 Fragments arranged by size: 4114 2377 197 AflIII A'CryG_T Cuts at: 1203 5587 6564 1203 Size: 4384 977 1327 Fragments arranged by size: 4384 1327 977 AgeI A'CCGG_T Cuts at: 5519 5519 Size: 6688 AhdI GACnn_n'nnGTC Cuts at: 5417 5417 Size: 6688 AleI CACnn'nnGTG Cuts at: 1166 3053 1166 Size: 1887 4801 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2552 1184 Size: 1368 5320 AlwNI CAG_nnn'CTG Cuts at: 2507 2716 2864 3622 3676 3912 4431 6003 2507 Size: 209 148 758 54 236 519 1572 3192 Fragments arranged by size: 3192 1572 758 519 236 209 148 54 ApaI G_GGCC'C Cuts at: 2501 3371 2501 Size: 870 5818 ApaLI G'TGCA_C Cuts at: 4745 5901 4745 Size: 1156 5532 ApoI r'AATT_y Cuts at: 1493 3997 4223 4375 6484 1493 Size: 2504 226 152 2109 1697 Fragments arranged by size: 2504 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5242 160 Size: 872 4210 1606 Fragments arranged by size: 4210 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 AvaI C'yCGr_G Cuts at: 1870 1943 2817 3688 4013 1870 Size: 73 874 871 325 4545 Fragments arranged by size: 4545 874 871 325 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2064 2097 2064 Size: 33 6655 BamHI G'GATC_C Cuts at: 2582 4018 4385 2582 Size: 1436 367 4885 Fragments arranged by size: 4885 1436 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2641 2927 3022 3061 3139 Size: 325 217 627 854 286 95 39 78 Cuts at: 3139 3161 3600 4009 5464 618 Size: 22 439 409 1455 1842 Fragments arranged by size: 1842 1455 854 627 439 409 325 286 217 95 78 39 22 BanII G_rGCy'C Cuts at: 1391 2501 3039 3371 4007 1391 Size: 1110 538 332 636 4072 Fragments arranged by size: 4072 1110 636 538 332 BbeI G_GCGC'C Cuts at: 3026 3026 Size: 6688 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2471 928 Size: 971 572 5145 Fragments arranged by size: 5145 971 572 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4879 4913 1903 Size: 34 2942 34 3678 Fragments arranged by size: 3678 2942 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2160 2379 4580 5790 2160 Size: 219 2201 1210 3058 Fragments arranged by size: 3058 2201 1210 219 BclI T'GATC_A Cuts at: 1548 1548 Size: 6688 BfrBI ATG'CAT Cuts at: 3530 3530 Size: 6688 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2302 2424 5299 Size: 107 122 71 631 605 630 122 2875 Cuts at: 5299 136 Size: 1525 Fragments arranged by size: 2875 1525 631 630 605 122 122 107 71 BglII A'GATC_T Cuts at: 3585 6683 3585 Size: 3098 3590 BlpI GC'TnA_GC Cuts at: 2513 2617 4105 2513 Size: 104 1488 5096 Fragments arranged by size: 5096 1488 104 Bme1580I G_kGCm'C Cuts at: 1790 2501 2546 2824 2943 3371 4749 5905 1790 Size: 711 45 278 119 428 1378 1156 2573 Fragments arranged by size: 2573 1378 1156 711 428 278 119 45 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 2659 2852 Size: 416 53 83 399 24 1078 164 193 Cuts at: 2852 2912 3883 4419 5377 442 Size: 60 971 536 958 1753 Fragments arranged by size: 1753 1078 971 958 536 416 399 193 164 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2206 2714 2867 3171 5348 1239 Size: 967 508 153 304 2177 2579 Fragments arranged by size: 2579 2177 967 508 304 153 Bpu10I CC'TnA_GC Cuts at: 3306 3306 Size: 6688 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2095 2192 4813 5678 5976 2095 Size: 97 2621 865 298 2807 Fragments arranged by size: 2807 2621 865 298 97 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 3272 5351 882 Size: 617 940 833 2079 2219 Fragments arranged by size: 2219 2079 940 833 617 BsaAI yAC'GTr Cuts at: 493 1204 4479 493 Size: 711 3275 2702 Fragments arranged by size: 3275 2702 711 BsaBI GATnn'nnATC Cuts at: 6682 6682 Size: 6688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3023 4495 4877 5512 Size: 53 83 186 936 1490 1472 382 635 Cuts at: 5512 275 Size: 1451 Fragments arranged by size: 1490 1472 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3515 5121 5519 5793 5940 6351 3515 Size: 1606 398 274 147 411 3852 Fragments arranged by size: 3852 1606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2179 2209 1178 Size: 30 165 30 776 30 5657 Fragments arranged by size: 5657 776 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3959 1186 Size: 2773 3915 BseYI C'CCAG_C Cuts at: 2539 3615 3641 3646 3750 5891 6387 2539 Size: 1076 26 5 104 2141 496 2840 Fragments arranged by size: 2840 2141 1076 496 104 26 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3072 3077 3116 3234 3650 1163 Size: 573 1336 5 39 118 416 4201 Fragments arranged by size: 4201 1336 573 416 118 39 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4138 4899 5927 1540 Size: 275 186 2137 761 1028 2301 Fragments arranged by size: 2301 2137 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3119 4749 4834 5905 3119 Size: 1630 85 1071 3902 Fragments arranged by size: 3902 1630 1071 85 BsmI GAATG_Cn' Cuts at: 4199 4292 4199 Size: 93 6595 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2501 2546 2824 2943 3039 3119 3371 Size: 399 711 45 278 119 96 80 252 Cuts at: 3371 4007 4749 4834 5905 1391 Size: 636 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 711 636 399 278 252 119 96 85 80 45 BspEI T'CCGG_A Cuts at: 6351 6351 Size: 6688 BspHI T'CATG_A Cuts at: 4577 6307 4577 Size: 1730 4958 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2296 3049 3217 3778 3973 4043 844 Size: 1452 753 168 561 195 70 3489 Fragments arranged by size: 3489 1452 753 561 195 168 70 BsrBI CCG'CTC Cuts at: 1394 1823 4575 1394 Size: 429 2752 3507 Fragments arranged by size: 3507 2752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2541 5183 5357 65 Size: 1183 1293 2642 174 1396 Fragments arranged by size: 2642 1396 1293 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5332 5519 1700 Size: 110 3522 187 2869 Fragments arranged by size: 3522 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6688 BssSI C'ACGA_G Cuts at: 4748 5760 4748 Size: 1012 5676 BstAPI GCAn_nnn'nTGC Cuts at: 794 3508 3936 794 Size: 2714 428 3546 Fragments arranged by size: 3546 2714 428 BstBI TT'CG_AA Cuts at: 5492 5492 Size: 6688 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6688 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3733 2011 Size: 1722 4966 BstYI r'GATC_y Cuts at: 1972 2008 2582 3495 3585 4018 4385 4770 4787 Size: 36 574 913 90 433 367 385 17 Cuts at: 4787 6239 6325 6337 6683 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 913 574 433 385 367 346 90 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2553 513 Size: 552 939 549 4648 Fragments arranged by size: 4648 939 552 549 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2558 3576 487 Size: 683 56 1332 1018 3599 Fragments arranged by size: 3599 1332 1018 683 56 BtsI GCAGTG_nn' Cuts at: 2862 3084 3744 4204 4998 5018 2862 Size: 222 660 460 794 20 4532 Fragments arranged by size: 4532 794 660 460 222 20 ClaI AT'CG_AT Cuts at: 1554 3439 1554 Size: 1885 4803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6653 DraI TTT'AAA Cuts at: 3992 3992 Size: 6688 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5695 809 Size: 507 4379 1802 Fragments arranged by size: 4379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2977 4135 5024 Size: 54 1425 50 275 18 1147 1158 889 Cuts at: 5024 6653 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1158 1147 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4135 1537 Size: 275 2323 4090 Fragments arranged by size: 4090 2323 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 3401 4618 1353 Size: 1082 966 1217 3423 Fragments arranged by size: 3423 1217 1082 966 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5275 5649 5795 387 Size: 1064 138 3686 374 146 1280 Fragments arranged by size: 3686 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2714 2781 2867 3093 3171 3333 Size: 393 574 508 67 86 226 78 162 Cuts at: 3333 3698 4751 5348 6135 6403 1239 Size: 365 1053 597 787 268 1524 Fragments arranged by size: 1524 1053 787 597 574 508 393 365 268 226 162 86 78 67 EcoNI CCTnn'n_nnAGG Cuts at: 2137 3785 2137 Size: 1648 5040 EcoO109I rG'GnC_Cy Cuts at: 2497 2959 3368 2497 Size: 462 409 5817 Fragments arranged by size: 5817 462 409 EcoRI G'AATT_C Cuts at: 3997 4375 3997 Size: 378 6310 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 3797 3892 2464 Size: 1333 95 5260 Fragments arranged by size: 5260 1333 95 FspI TGC'GCA Cuts at: 5194 5194 Size: 6688 HaeII r_GCGC'y Cuts at: 1678 3026 5835 1678 Size: 1348 2809 2531 Fragments arranged by size: 2809 2531 1348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2179 2211 3169 3201 3314 Size: 32 135 32 607 32 958 32 113 Cuts at: 3314 3346 3575 3607 5329 5361 5403 5435 1373 Size: 32 229 32 1722 32 42 32 2626 Fragments arranged by size: 2626 1722 958 607 229 135 113 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 3183 4032 4278 669 Size: 988 281 382 863 849 246 3079 Fragments arranged by size: 3079 988 863 849 382 281 246 HindIII A'AGCT_T Cuts at: 748 2446 748 Size: 1698 4990 HpaI GTT'AAC Cuts at: 4278 4278 Size: 6688 KasI G'GCGC_C Cuts at: 3022 3022 Size: 6688 KpnI G_GTAC'C Cuts at: 3165 4013 3165 Size: 848 5840 MfeI C'AATT_G Cuts at: 3222 4287 3222 Size: 1065 5623 MluI A'CGCG_T Cuts at: 6564 6564 Size: 6688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5802 5986 1305 Size: 4497 184 2007 Fragments arranged by size: 4497 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2979 10 Size: 54 1425 343 1147 3719 Fragments arranged by size: 3719 1425 1147 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 3053 3122 4646 5005 5164 518 Size: 648 1392 495 69 1524 359 159 2042 Fragments arranged by size: 2042 1524 1392 648 495 359 159 69 MspA1I CmG'CkG Cuts at: 1606 2146 2975 3248 3619 3658 3796 4104 4781 Size: 540 829 273 371 39 138 308 677 Cuts at: 4781 5929 6174 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 540 371 308 273 245 138 39 NarI GG'CG_CC Cuts at: 3023 3023 Size: 6688 NcoI C'CATG_G Cuts at: 513 1065 2004 2553 513 Size: 552 939 549 4648 Fragments arranged by size: 4648 939 552 549 NdeI CA'TA_TG Cuts at: 387 387 Size: 6688 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6688 NotI GC'GGCC_GC Cuts at: 4135 4135 Size: 6688 NsiI A_TGCA'T Cuts at: 3532 3532 Size: 6688 NspI r_CATG'y Cuts at: 1132 1229 4046 5591 1132 Size: 97 2817 1545 2229 Fragments arranged by size: 2817 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3364 1348 Size: 2016 4672 PciI A'CATG_T Cuts at: 5587 5587 Size: 6688 PflMI CCAn_nnn'nTGG Cuts at: 1108 2133 2507 1108 Size: 1025 374 5289 Fragments arranged by size: 5289 1025 374 PfoI T'CCnGG_A Cuts at: 2585 2585 Size: 6688 PmeI GTTT'AAAC Cuts at: 3992 3992 Size: 6688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4757 4789 6270 6302 4757 Size: 32 1481 32 5143 Fragments arranged by size: 5143 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6688 PsiI TTA'TAA Cuts at: 4258 4258 Size: 6688 PspOMI G'GGCC_C Cuts at: 2497 3367 2497 Size: 870 5818 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3343 3375 3343 Size: 32 6656 PstI C_TGCA'G Cuts at: 830 2883 2956 3034 3112 3214 3504 4040 830 Size: 2053 73 78 78 102 290 536 3478 Fragments arranged by size: 3478 2053 536 290 102 78 78 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6688 PvuII CAG'CTG Cuts at: 2146 2975 3248 3658 2146 Size: 829 273 410 5176 Fragments arranged by size: 5176 829 410 273 SalI G'TCGA_C Cuts at: 1936 4030 1936 Size: 2094 4594 SbfI CC_TGCA'GG Cuts at: 2883 3504 4040 2883 Size: 621 536 5531 Fragments arranged by size: 5531 621 536 ScaI AGT'ACT Cuts at: 4936 6648 4936 Size: 1712 4976 SexAI A'CCwGG_T Cuts at: 2637 3013 2637 Size: 376 6312 SfcI C'TryA_G Cuts at: 826 1045 2879 2952 2991 3030 3108 3195 3210 Size: 219 1834 73 39 39 78 87 15 Cuts at: 3210 3264 3500 4036 5171 5852 6043 826 Size: 54 236 536 1135 681 191 1471 Fragments arranged by size: 1834 1471 1135 681 536 236 219 191 87 78 73 54 39 39 15 SfoI GGC'GCC Cuts at: 3024 3024 Size: 6688 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6688 SmaI CCC'GGG Cuts at: 1872 4015 1872 Size: 2143 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2110 2207 3394 4792 5693 5955 Size: 197 926 167 97 1187 1398 901 262 Cuts at: 5955 820 Size: 1553 Fragments arranged by size: 1553 1398 1187 926 901 262 197 167 97 SnaBI TAC'GTA Cuts at: 493 4479 493 Size: 3986 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6688 SphI G_CATG'C Cuts at: 1132 4046 1132 Size: 2914 3774 SspI AAT'ATT Cuts at: 5 52 4612 5 Size: 47 4560 2081 Fragments arranged by size: 4560 2081 47 StuI AGG'CCT Cuts at: 1517 3780 1517 Size: 2263 4425 StyI C'CwwG_G Cuts at: 513 1065 2004 2553 2676 3553 3567 3781 4127 Size: 552 939 549 123 877 14 214 346 Cuts at: 4127 513 Size: 3074 Fragments arranged by size: 3074 939 877 552 549 346 214 123 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3262 3764 4715 4732 4885 5070 5515 Size: 1255 1682 502 951 17 153 185 445 Cuts at: 5515 325 Size: 1498 Fragments arranged by size: 1682 1498 1255 951 502 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4934 6646 96 Size: 274 80 33 51 1443 2957 1712 138 Fragments arranged by size: 2957 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2349 2381 6648 6680 2349 Size: 32 4267 32 2357 Fragments arranged by size: 4267 2357 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3470 3544 1316 Size: 2154 74 4460 Fragments arranged by size: 4460 2154 74 XbaI T'CTAG_A Cuts at: 4024 4024 Size: 6688 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2099 2427 2744 3397 2099 Size: 328 317 653 5390 Fragments arranged by size: 5390 653 328 317 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6688 XmaI C'CCGG_G Cuts at: 1870 4013 1870 Size: 2143 4545 XmnI GAAnn'nnTTC Cuts at: 4817 4817 Size: 6688 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4496 276 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI