(Linear) (Six-base) MAPSORT of: KIEE4551.seq Check: 5008 from: 1 to: 1983 KIEE4551.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1044 1212 1983 Size: 1044 168 771 Fragments arranged by size: 1044 771 168 AccI GT'mk_AC Cuts at: 0 563 1336 1917 1983 Size: 563 773 581 66 Fragments arranged by size: 773 581 563 66 Acc65I G'GTAC_C Cuts at: 0 1156 1983 Size: 1156 827 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 776 1088 1328 1693 1983 Size: 776 312 240 365 290 Fragments arranged by size: 776 365 312 290 240 AflII C'TTAA_G Cuts at: 0 1389 1983 Size: 1389 594 AleI CACnn'nnGTG Cuts at: 0 1048 1983 Size: 1048 935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 547 1983 Size: 547 1436 AlwNI CAG_nnn'CTG Cuts at: 0 502 711 859 1617 1671 1907 1983 Size: 502 209 148 758 54 236 76 Fragments arranged by size: 758 502 236 209 148 76 54 ApaI G_GGCC'C Cuts at: 0 496 1366 1983 Size: 496 870 617 Fragments arranged by size: 870 617 496 AvaI C'yCGr_G Cuts at: 0 812 1683 1983 Size: 812 871 300 Fragments arranged by size: 871 812 300 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 59 92 1983 Size: 59 33 1891 Fragments arranged by size: 1891 59 33 BamHI G'GATC_C Cuts at: 0 577 1983 Size: 577 1406 BanI G'GyrC_C Cuts at: 0 636 922 1017 1056 1134 1156 1595 1983 Size: 636 286 95 39 78 22 439 388 Fragments arranged by size: 636 439 388 286 95 78 39 22 BanII G_rGCy'C Cuts at: 0 496 1034 1366 1983 Size: 496 538 332 617 Fragments arranged by size: 617 538 496 332 BbeI G_GCGC'C Cuts at: 0 1021 1983 Size: 1021 962 BbsI GAAGACnn'nnnn_ Cuts at: 0 466 1983 Size: 466 1517 BciVI GTATCCnnnnn_n' Cuts at: 0 155 374 1983 Size: 155 219 1609 Fragments arranged by size: 1609 219 155 BfrBI ATG'CAT Cuts at: 0 1525 1983 Size: 1525 458 BglI GCCn_nnn'nGGC Cuts at: 0 297 419 1983 Size: 297 122 1564 Fragments arranged by size: 1564 297 122 BglII A'GATC_T Cuts at: 0 1580 1983 Size: 1580 403 BlpI GC'TnA_GC Cuts at: 0 508 612 1983 Size: 508 104 1371 Fragments arranged by size: 1371 508 104 Bme1580I G_kGCm'C Cuts at: 0 496 541 819 938 1366 1983 Size: 496 45 278 119 428 617 Fragments arranged by size: 617 496 428 278 119 45 BmrI ACTGGGnnnn_n' Cuts at: 0 490 654 847 907 1878 1983 Size: 490 164 193 60 971 105 Fragments arranged by size: 971 490 193 164 105 60 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 709 862 1166 1983 Size: 201 508 153 304 817 Fragments arranged by size: 817 508 304 201 153 Bpu10I CC'TnA_GC Cuts at: 0 1301 1983 Size: 1301 682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 90 187 1983 Size: 90 97 1796 Fragments arranged by size: 1796 97 90 BsaI GGTCTCn'nnnn_ Cuts at: 0 434 1267 1983 Size: 434 833 716 Fragments arranged by size: 833 716 434 BsaHI Gr'CG_yC Cuts at: 0 1018 1983 Size: 1018 965 BsaWI w'CCGG_w Cuts at: 0 1510 1983 Size: 1510 473 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 174 204 1983 Size: 174 30 1779 Fragments arranged by size: 1779 174 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1954 1983 Size: 1954 29 BseYI C'CCAG_C Cuts at: 0 534 1610 1636 1641 1745 1983 Size: 534 1076 26 5 104 238 Fragments arranged by size: 1076 534 238 104 26 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1067 1072 1111 1229 1645 1983 Size: 1067 5 39 118 416 338 Fragments arranged by size: 1067 416 338 118 39 5 BsiHKAI G_wGCw'C Cuts at: 0 1114 1983 Size: 1114 869 Bsp1286I G_dGCh'C Cuts at: 0 496 541 819 938 1034 1114 1366 1983 Size: 496 45 278 119 96 80 252 617 Fragments arranged by size: 617 496 278 252 119 96 80 45 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 291 1044 1212 1773 1968 1983 Size: 291 753 168 561 195 15 Fragments arranged by size: 753 561 291 195 168 15 BsrDI GCAATG_nn' Cuts at: 0 536 1983 Size: 536 1447 BstAPI GCAn_nnn'nTGC Cuts at: 0 1503 1931 1983 Size: 1503 428 52 Fragments arranged by size: 1503 428 52 BstXI CCAn_nnnn'nTGG Cuts at: 0 1728 1983 Size: 1728 255 BstYI r'GATC_y Cuts at: 0 3 577 1490 1580 1983 Size: 3 574 913 90 403 Fragments arranged by size: 913 574 403 90 3 BtgI C'CryG_G Cuts at: 0 548 1983 Size: 548 1435 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 553 1571 1983 Size: 553 1018 412 Fragments arranged by size: 1018 553 412 BtsI GCAGTG_nn' Cuts at: 0 857 1079 1739 1983 Size: 857 222 660 244 Fragments arranged by size: 857 660 244 222 ClaI AT'CG_AT Cuts at: 0 1434 1983 Size: 1434 549 EaeI y'GGCC_r Cuts at: 0 972 1983 Size: 972 1011 EarI CTCTTCn'nnn_ Cuts at: 0 430 1396 1983 Size: 430 966 587 Fragments arranged by size: 966 587 430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 709 776 862 1088 1166 1328 1693 Size: 201 508 67 86 226 78 162 365 Cuts at: 1693 1983 Size: 290 Fragments arranged by size: 508 365 290 226 201 162 86 78 67 EcoNI CCTnn'n_nnAGG Cuts at: 0 132 1780 1983 Size: 132 1648 203 Fragments arranged by size: 1648 203 132 EcoO109I rG'GnC_Cy Cuts at: 0 492 954 1363 1983 Size: 492 462 409 620 Fragments arranged by size: 620 492 462 409 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 459 1792 1887 1983 Size: 459 1333 95 96 Fragments arranged by size: 1333 459 96 95 HaeII r_GCGC'y Cuts at: 0 1021 1983 Size: 1021 962 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 174 206 1164 1196 1309 1341 1570 1602 Size: 174 32 958 32 113 32 229 32 Cuts at: 1602 1983 Size: 381 Fragments arranged by size: 958 381 229 174 113 32 32 32 32 HincII GTy'rAC Cuts at: 0 315 1178 1983 Size: 315 863 805 Fragments arranged by size: 863 805 315 HindIII A'AGCT_T Cuts at: 0 441 1983 Size: 441 1542 KasI G'GCGC_C Cuts at: 0 1017 1983 Size: 1017 966 KpnI G_GTAC'C Cuts at: 0 1160 1983 Size: 1160 823 MfeI C'AATT_G Cuts at: 0 1217 1983 Size: 1217 766 MscI TGG'CCA Cuts at: 0 974 1983 Size: 974 1009 MslI CAynn'nnrTG Cuts at: 0 553 1048 1117 1983 Size: 553 495 69 866 Fragments arranged by size: 866 553 495 69 MspA1I CmG'CkG Cuts at: 0 141 970 1243 1614 1653 1791 1983 Size: 141 829 273 371 39 138 192 Fragments arranged by size: 829 371 273 192 141 138 39 NarI GG'CG_CC Cuts at: 0 1018 1983 Size: 1018 965 NcoI C'CATG_G Cuts at: 0 548 1983 Size: 548 1435 NsiI A_TGCA'T Cuts at: 0 1527 1983 Size: 1527 456 PasI CC'CwG_GG Cuts at: 0 1359 1983 Size: 1359 624 PflMI CCAn_nnn'nTGG Cuts at: 0 128 502 1983 Size: 128 374 1481 Fragments arranged by size: 1481 374 128 PfoI T'CCnGG_A Cuts at: 0 580 1983 Size: 580 1403 PspOMI G'GGCC_C Cuts at: 0 492 1362 1983 Size: 492 870 621 Fragments arranged by size: 870 621 492 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1338 1370 1983 Size: 1338 32 613 Fragments arranged by size: 1338 613 32 PstI C_TGCA'G Cuts at: 0 878 951 1029 1107 1209 1499 1983 Size: 878 73 78 78 102 290 484 Fragments arranged by size: 878 484 290 102 78 78 73 PvuII CAG'CTG Cuts at: 0 141 970 1243 1653 1983 Size: 141 829 273 410 330 Fragments arranged by size: 829 410 330 273 141 SbfI CC_TGCA'GG Cuts at: 0 878 1499 1983 Size: 878 621 484 Fragments arranged by size: 878 621 484 SexAI A'CCwGG_T Cuts at: 0 632 1008 1983 Size: 632 376 975 Fragments arranged by size: 975 632 376 SfcI C'TryA_G Cuts at: 0 874 947 986 1025 1103 1190 1205 1259 Size: 874 73 39 39 78 87 15 54 Cuts at: 1259 1495 1983 Size: 236 488 Fragments arranged by size: 874 488 236 87 78 73 54 39 39 15 SfoI GGC'GCC Cuts at: 0 1019 1983 Size: 1019 964 SmlI C'TyrA_G Cuts at: 0 105 202 1389 1983 Size: 105 97 1187 594 Fragments arranged by size: 1187 594 105 97 StuI AGG'CCT Cuts at: 0 1775 1983 Size: 1775 208 StyI C'CwwG_G Cuts at: 0 548 671 1548 1562 1776 1983 Size: 548 123 877 14 214 207 Fragments arranged by size: 877 548 214 207 123 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1257 1759 1983 Size: 1257 502 224 Fragments arranged by size: 1257 502 224 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 344 376 1983 Size: 344 32 1607 Fragments arranged by size: 1607 344 32 Tth111I GACn'n_nGTC Cuts at: 0 1465 1539 1983 Size: 1465 74 444 Fragments arranged by size: 1465 444 74 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 94 422 739 1392 1983 Size: 94 328 317 653 591 Fragments arranged by size: 653 591 328 317 94 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AleI AlfI AlwNI ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI MfeI MscI MslI MspA1I NarI NcoI NsiI PasI PflMI PfoI PspOMI PsrI PstI PvuII SbfI SexAI SfcI SfoI SmlI StuI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AatII AclI AfeI AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BbvCI BcgI BclI BmgBI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI ZraI