################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaJI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MluI MroI MseI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 28 ACATGT A^C[GA][CT]GT BalI 173 TGGCCA TGG^CCA BamHI 506 GGATCC G^GATCC BanII 361 GAGCTC G[GA]GC[CT]^C BbrPI 720 CACGTG CAC^GTG BbsI 21 GAAGAC GAAGAC(2/6) BciVI 323 GTATCC GTATCC(6/5) BcnI 409 CCGGG CC^[GC]GG BpuAI 21 GAAGAC GAAGAC(2/6) BsaAI 720 CACGTG [CT]AC^GT[GA] BseRI 289 CTCCTC GAGGAG(10/8) BsgI 697 GTGCAG GTGCAG(16/14) BsmFI 538 GTCCC GGGAC(10/14) Bsp1407I 731 TGTACA T^GTACA BspLU11I 28 ACATGT A^CATGT BsrBI 510 CCGCTC CCGCTC(-3/-3) BsrDI 687 CATTGC GCAATG(2/0) BsrFI 523 GCCGGT [GA]^CCGG[CT] BsrGI 731 TGTACA T^GTACA BstAPI 35 GCACCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 506 GGATCC [GA]^GATC[CT] Cfr10I 523 GCCGGT [GA]^CCGG[CT] FauI 199 GCGGG CCCGC(4/6) HinfI 738 GAGTC G^A[ACGT]TC MaeII 721 ACGT A^CGT MfeI 69 CAATTG C^AATTG MflI 506 GGATCC [GA]^GATC[CT] MluNI 173 TGGCCA TGG^CCA MscI 173 TGGCCA TGG^CCA MunI 69 CAATTG C^AATTG NciI 409 CCGGG CC^[GC]GG NspI 28 ACATGT [GA]CATG^[CT] PleI 738 GAGTC GAGTC(4/5) PmaCI 720 CACGTG CAC^GTG PmlI 720 CACGTG CAC^GTG PshAI 219 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 445 CTGCAG CTGCA^G SacI 361 GAGCTC GAGCT^C SfcI 445 CTGCAG C^T[GA][CT]AG SspBI 731 TGTACA T^GTACA Tsp509I 70 AATT ^AATT XhoII 506 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 346 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 546 CAGCTGCTG AocI 158 CCTCAGG CC^T[ACGT]AGG 657 CCTGAGG Aor51HI 395 AGCGCT AGC^GCT 430 AGCGCT AvaII 438 GGACC G^G[AT]CC 537 GGTCC BanI 34 GGCACC G^G[CT][GA]CC 163 GGCACC BpmI 307 CTGGAG CTGGAG(16/14) 748 CTGGAG BsaI 584 GAGACC GGTCTC(1/5) 637 GAGACC BsrI 220 ACTGG ACTGG(1/-1) 488 ACTGG BstXI 428 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 459 CCACTGCTGTGG Bsu36I 158 CCTCAGG CC^T[ACGT]AGG 657 CCTGAGG DdeI 159 CTCAG C^T[ACGT]AG 658 CTGAG EaeI 173 TGGCCA [CT]^GGCC[GA] 528 TGGCCG Eco47III 395 AGCGCT AGC^GCT 430 AGCGCT Eco81I 158 CCTCAGG CC^T[ACGT]AGG 657 CCTGAGG HaeII 395 AGCGCT [GA]GCGC^[CT] 430 AGCGCT HapII 409 CCGG C^CGG 524 CCGG HgaI 120 GACGC GACGC(5/10) 255 GCGTC HpaII 409 CCGG C^CGG 524 CCGG MspI 409 CCGG C^CGG 524 CCGG SfaNI 113 GCATC GCATC(5/9) 623 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BbvI BmyI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SmlI TseI Tsp45I TspRI