(Circular) (Six-base) MAPSORT of: pFN21AE1379.seq Check: 5256 from: 1 to: 5464 pFN21AE1379 5464 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2402 3527 4911 5179 1632 Size: 770 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 770 268 AfeI AGC'GCT Cuts at: 2402 2437 2402 Size: 35 5429 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 2033 4363 5340 1203 Size: 830 2330 977 1327 Fragments arranged by size: 2330 1327 977 830 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 2469 1166 Size: 1303 4161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AlwNI CAG_nnn'CTG Cuts at: 2356 2556 3207 4779 2356 Size: 200 651 1572 3041 Fragments arranged by size: 3041 1572 651 200 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2789 1870 Size: 73 846 4545 Fragments arranged by size: 4545 846 73 BamHI G'GATC_C Cuts at: 2511 2794 3161 2511 Size: 283 367 4814 Fragments arranged by size: 4814 367 283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2168 2785 4240 618 Size: 325 217 627 252 129 617 1455 1842 Fragments arranged by size: 1842 1455 627 617 325 252 217 129 BanII G_rGCy'C Cuts at: 1391 2370 2783 1391 Size: 979 413 4072 Fragments arranged by size: 4072 979 413 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2033 928 Size: 971 134 4359 Fragments arranged by size: 4359 971 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2339 3356 4566 2339 Size: 1017 1210 3237 Fragments arranged by size: 3237 1210 1017 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4075 136 Size: 107 122 71 631 605 2403 1525 Fragments arranged by size: 2403 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 2210 3525 4681 1790 Size: 420 1315 1156 2573 Fragments arranged by size: 2573 1315 1156 420 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2235 3195 4153 Size: 416 53 83 399 24 818 960 958 Cuts at: 4153 442 Size: 1753 Fragments arranged by size: 1753 960 958 818 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2774 4124 1239 Size: 1094 441 1350 2579 Fragments arranged by size: 2579 1350 1094 441 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2036 2120 2354 2732 3589 4454 4752 2036 Size: 84 234 378 857 865 298 2748 Fragments arranged by size: 2748 865 857 378 298 234 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2583 2636 4127 882 Size: 617 1084 53 1491 2219 Fragments arranged by size: 2219 1491 1084 617 53 BsaAI yAC'GTr Cuts at: 493 1204 2727 3255 493 Size: 711 1523 528 2702 Fragments arranged by size: 2702 1523 711 528 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2582 2612 1178 Size: 30 165 30 1179 30 4030 Fragments arranged by size: 4030 1179 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 1186 Size: 1099 4365 BseYI C'CCAG_C Cuts at: 4667 5163 4667 Size: 496 4968 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2723 1163 Size: 573 987 3904 Fragments arranged by size: 3904 987 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2661 2760 3525 3610 4681 2370 Size: 291 99 765 85 1071 3153 Fragments arranged by size: 3153 1071 765 291 99 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2370 2661 2760 2783 3525 3610 Size: 399 420 160 291 99 23 742 85 Cuts at: 3610 4681 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 291 160 99 85 23 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 2277 2695 2819 844 Size: 1223 210 418 124 3489 Fragments arranged by size: 3489 1223 418 210 124 BsrBI CCG'CTC Cuts at: 1394 1823 2517 3351 1394 Size: 429 694 834 3507 Fragments arranged by size: 3507 834 694 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 3959 4133 65 Size: 1183 1443 1268 174 1396 Fragments arranged by size: 1443 1396 1268 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2528 4108 4295 1700 Size: 110 718 1580 187 2869 Fragments arranged by size: 2869 1580 718 187 110 BsrGI T'GTAC_A Cuts at: 96 2736 96 Size: 2640 2824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 2046 794 Size: 1252 4212 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstXI CCAn_nnnn'nTGG Cuts at: 2440 2471 2440 Size: 31 5433 BstYI r'GATC_y Cuts at: 1972 2511 2794 3161 3546 3563 5015 5101 5113 Size: 539 283 367 385 17 1452 86 12 Cuts at: 5113 5459 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 539 385 367 346 283 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2164 2663 2164 Size: 499 4965 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3973 Fragments arranged by size: 3973 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 487 Size: 683 56 1079 3646 Fragments arranged by size: 3646 1079 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2464 2980 3774 3794 2118 Size: 346 516 794 20 3788 Fragments arranged by size: 3788 794 516 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2533 2911 Size: 54 1425 50 275 18 348 355 378 Cuts at: 2911 3800 5429 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 378 355 348 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3394 1353 Size: 2041 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2368 2368 Size: 5464 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2402 2774 3527 4124 4911 5179 Size: 393 701 69 372 753 597 787 268 Cuts at: 5179 1239 Size: 1524 Fragments arranged by size: 1524 787 753 701 597 393 372 268 69 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2404 2439 4611 1678 Size: 726 35 2172 2531 Fragments arranged by size: 2531 2172 726 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 4105 4137 4179 Size: 32 135 32 1010 32 1491 32 42 Cuts at: 4179 4211 1373 Size: 32 2626 Fragments arranged by size: 2626 1491 1010 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 2074 3063 2074 Size: 989 4475 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2243 2619 2625 4578 4762 1305 Size: 938 376 6 1953 184 2007 Fragments arranged by size: 2007 1953 938 376 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 10 Size: 54 1425 343 348 3294 Fragments arranged by size: 3294 1425 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 2195 2469 3422 3781 3940 518 Size: 648 945 84 274 953 359 159 2042 Fragments arranged by size: 2042 953 945 648 359 274 159 84 MspA1I CmG'CkG Cuts at: 1606 2356 2449 2553 2880 3557 4705 4950 1606 Size: 750 93 104 327 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 677 327 245 104 93 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3973 Fragments arranged by size: 3973 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2037 2822 4367 1132 Size: 97 808 785 1545 2229 Fragments arranged by size: 2229 1545 808 785 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5464 PciI A'CATG_T Cuts at: 2033 4363 2033 Size: 2330 3134 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PfoI T'CCnGG_A Cuts at: 2652 2652 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PmlI CAC'GTG Cuts at: 2727 2727 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2228 1613 Size: 615 4849 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PstI C_TGCA'G Cuts at: 830 2454 2816 830 Size: 1624 362 3478 Fragments arranged by size: 3478 1624 362 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 PvuII CAG'CTG Cuts at: 2356 2449 2553 2356 Size: 93 104 5267 Fragments arranged by size: 5267 104 93 SacI G_AGCT'C Cuts at: 2370 2370 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2450 2812 3947 4628 4819 826 Size: 219 1405 362 1135 681 191 1471 Fragments arranged by size: 1471 1405 1135 681 362 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2791 1872 Size: 919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2051 2135 2369 2747 3568 4469 Size: 197 926 108 84 234 378 821 901 Cuts at: 4469 4731 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 821 378 262 234 197 108 84 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5464 StyI C'CwwG_G Cuts at: 513 1065 2004 2903 513 Size: 552 939 899 3074 Fragments arranged by size: 3074 939 899 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 3491 3508 3661 3846 4291 325 Size: 1255 417 1494 17 153 185 445 1498 Fragments arranged by size: 1498 1494 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 2736 3710 Size: 274 80 33 51 1443 303 456 974 Cuts at: 3710 5422 96 Size: 1712 138 Fragments arranged by size: 1712 1443 974 456 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2789 1870 Size: 919 4545 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI