(Linear) (Six-base) MAPSORT of: KIEE1433.seq Check: 1386 from: 1 to: 1497 KIEE1433.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 1497 Size: 25 1472 ApaI G_GGCC'C Cuts at: 0 954 1497 Size: 954 543 AvaI C'yCGr_G Cuts at: 0 806 1331 1497 Size: 806 525 166 Fragments arranged by size: 806 525 166 BanI G'GyrC_C Cuts at: 0 730 1126 1497 Size: 730 396 371 Fragments arranged by size: 730 396 371 BanII G_rGCy'C Cuts at: 0 817 954 1241 1357 1497 Size: 817 137 287 116 140 Fragments arranged by size: 817 287 140 137 116 BbsI GAAGACnn'nnnn_ Cuts at: 0 493 1497 Size: 493 1004 BbvCI CC'TCA_GC Cuts at: 0 743 1497 Size: 743 754 BciVI GTATCCnnnnn_n' Cuts at: 0 532 1497 Size: 532 965 BglI GCCn_nnn'nGGC Cuts at: 0 1306 1372 1497 Size: 1306 66 125 Fragments arranged by size: 1306 125 66 Bme1580I G_kGCm'C Cuts at: 0 733 954 1129 1497 Size: 733 221 175 368 Fragments arranged by size: 733 368 221 175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1260 1497 Size: 1260 237 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 1040 1497 Size: 462 578 457 Fragments arranged by size: 578 462 457 Bpu10I CC'TnA_GC Cuts at: 0 283 743 820 1497 Size: 283 460 77 677 Fragments arranged by size: 677 460 283 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 1226 1387 1497 Size: 1226 161 110 Fragments arranged by size: 1226 161 110 BsaHI Gr'CG_yC Cuts at: 0 316 1497 Size: 316 1181 BseYI C'CCAG_C Cuts at: 0 947 1031 1055 1497 Size: 947 84 24 442 Fragments arranged by size: 947 442 84 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 769 784 1481 1497 Size: 13 756 15 697 16 Fragments arranged by size: 756 697 16 15 13 BsiHKAI G_wGCw'C Cuts at: 0 1017 1165 1210 1241 1497 Size: 1017 148 45 31 256 Fragments arranged by size: 1017 256 148 45 31 Bsp1286I G_dGCh'C Cuts at: 0 733 817 954 1017 1129 1165 1210 1241 Size: 733 84 137 63 112 36 45 31 Cuts at: 1241 1357 1497 Size: 116 140 Fragments arranged by size: 733 140 137 116 112 84 63 45 36 31 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 35 499 1497 Size: 35 464 998 Fragments arranged by size: 998 464 35 BsrBI CCG'CTC Cuts at: 0 1446 1497 Size: 1446 51 BsrFI r'CCGG_y Cuts at: 0 154 1497 Size: 154 1343 BssHII G'CGCG_C Cuts at: 0 76 1497 Size: 76 1421 BstAPI GCAn_nnn'nTGC Cuts at: 0 36 73 657 1497 Size: 36 37 584 840 Fragments arranged by size: 840 584 37 36 BstBI TT'CG_AA Cuts at: 0 783 1497 Size: 783 714 BstEII G'GTnAC_C Cuts at: 0 226 1497 Size: 226 1271 BstXI CCAn_nnnn'nTGG Cuts at: 0 1110 1497 Size: 1110 387 BstYI r'GATC_y Cuts at: 0 386 1497 Size: 386 1111 Bsu36I CC'TnA_GG Cuts at: 0 1399 1497 Size: 1399 98 BtgI C'CryG_G Cuts at: 0 596 1184 1251 1295 1497 Size: 596 588 67 44 202 Fragments arranged by size: 596 588 202 67 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 229 1497 Size: 229 1268 BtsI GCAGTG_nn' Cuts at: 0 255 744 1497 Size: 255 489 753 Fragments arranged by size: 753 489 255 DraIII CAC_nnn'GTG Cuts at: 0 903 916 1497 Size: 903 13 581 Fragments arranged by size: 903 581 13 EaeI y'GGCC_r Cuts at: 0 269 593 861 905 918 971 1298 1497 Size: 269 324 268 44 13 53 327 199 Fragments arranged by size: 327 324 269 268 199 53 44 13 EcoICRI GAG'CTC Cuts at: 0 1239 1497 Size: 1239 258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 462 1040 1497 Size: 25 437 578 457 Fragments arranged by size: 578 457 437 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 286 1497 Size: 286 1211 EcoO109I rG'GnC_Cy Cuts at: 0 951 1169 1335 1497 Size: 951 218 166 162 Fragments arranged by size: 951 218 166 162 HaeII r_GCGC'y Cuts at: 0 155 1413 1497 Size: 155 1258 84 Fragments arranged by size: 1258 155 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 48 1497 Size: 16 32 1449 Fragments arranged by size: 1449 32 16 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1403 1497 Size: 1403 94 MscI TGG'CCA Cuts at: 0 271 863 907 920 973 1300 1497 Size: 271 592 44 13 53 327 197 Fragments arranged by size: 592 327 271 197 53 44 13 MslI CAynn'nnrTG Cuts at: 0 248 875 878 1497 Size: 248 627 3 619 Fragments arranged by size: 627 619 248 3 MspA1I CmG'CkG Cuts at: 0 45 1497 Size: 45 1452 NaeI GCC'GGC Cuts at: 0 156 1497 Size: 156 1341 NcoI C'CATG_G Cuts at: 0 1251 1295 1497 Size: 1251 44 202 Fragments arranged by size: 1251 202 44 NgoMIV G'CCGG_C Cuts at: 0 154 1497 Size: 154 1343 PflMI CCAn_nnn'nTGG Cuts at: 0 1217 1497 Size: 1217 280 PfoI T'CCnGG_A Cuts at: 0 524 1017 1497 Size: 524 493 480 Fragments arranged by size: 524 493 480 PpuMI rG'GwC_Cy Cuts at: 0 1335 1497 Size: 1335 162 PspOMI G'GGCC_C Cuts at: 0 950 1497 Size: 950 547 PstI C_TGCA'G Cuts at: 0 653 1046 1497 Size: 653 393 451 Fragments arranged by size: 653 451 393 PvuII CAG'CTG Cuts at: 0 45 1497 Size: 45 1452 SacI G_AGCT'C Cuts at: 0 1241 1497 Size: 1241 256 ScaI AGT'ACT Cuts at: 0 449 1497 Size: 449 1048 SfcI C'TryA_G Cuts at: 0 453 649 1042 1497 Size: 453 196 393 455 Fragments arranged by size: 455 453 393 196 SmaI CCC'GGG Cuts at: 0 1333 1497 Size: 1333 164 StyI C'CwwG_G Cuts at: 0 95 194 1251 1295 1301 1485 1497 Size: 95 99 1057 44 6 184 12 Fragments arranged by size: 1057 184 99 95 44 12 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 69 138 1069 1497 Size: 69 69 931 428 Fragments arranged by size: 931 428 69 69 TatI w'GTAC_w Cuts at: 0 447 588 1497 Size: 447 141 909 Fragments arranged by size: 909 447 141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 934 1302 1497 Size: 934 368 195 Fragments arranged by size: 934 368 195 XmaI C'CCGG_G Cuts at: 0 1331 1497 Size: 1331 166 Enzymes that do cut: AcuI ApaI AvaI BanI BanII BbsI BbvCI BciVI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaHI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PflMI PfoI PpuMI PspOMI PstI PvuII SacI ScaI SfcI SmaI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstZ17I ClaI CspCI DraI DrdI EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI