Restriction map for pFN21AE1433
 (Linear) (Six-base) MAPSORT of: KIEE1433.seq  Check: 1386  from: 1  to: 1497



KIEE1433.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25    1497 
   Size:         25    1472
 
ApaI G_GGCC'C

Cuts at:      0     954    1497 
   Size:        954     543
 
AvaI C'yCGr_G

Cuts at:      0     806    1331    1497 
   Size:        806     525     166

  Fragments arranged by size: 

                 806     525     166
 
BanI G'GyrC_C

Cuts at:      0     730    1126    1497 
   Size:        730     396     371

  Fragments arranged by size: 

                 730     396     371
 
BanII G_rGCy'C

Cuts at:      0     817     954    1241    1357    1497 
   Size:        817     137     287     116     140

  Fragments arranged by size: 

                 817     287     140     137     116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     493    1497 
   Size:        493    1004
 
BbvCI CC'TCA_GC

Cuts at:      0     743    1497 
   Size:        743     754
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     532    1497 
   Size:        532     965
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1306    1372    1497 
   Size:       1306      66     125

  Fragments arranged by size: 

                1306     125      66
 
Bme1580I G_kGCm'C

Cuts at:      0     733     954    1129    1497 
   Size:        733     221     175     368

  Fragments arranged by size: 

                 733     368     221     175
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1260    1497 
   Size:       1260     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462    1040    1497 
   Size:        462     578     457

  Fragments arranged by size: 

                 578     462     457
 
Bpu10I CC'TnA_GC

Cuts at:      0     283     743     820    1497 
   Size:        283     460      77     677

  Fragments arranged by size: 

                 677     460     283      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1226    1387    1497 
   Size:       1226     161     110

  Fragments arranged by size: 

                1226     161     110
 
BsaHI Gr'CG_yC

Cuts at:      0     316    1497 
   Size:        316    1181
 
BseYI C'CCAG_C

Cuts at:      0     947    1031    1055    1497 
   Size:        947      84      24     442

  Fragments arranged by size: 

                 947     442      84      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     769     784    1481    1497 
   Size:         13     756      15     697      16

  Fragments arranged by size: 

                 756     697      16      15      13
 
BsiHKAI G_wGCw'C

Cuts at:      0    1017    1165    1210    1241    1497 
   Size:       1017     148      45      31     256

  Fragments arranged by size: 

                1017     256     148      45      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     733     817     954    1017    1129    1165    1210    1241 
   Size:        733      84     137      63     112      36      45      31

Cuts at:   1241    1357    1497 
   Size:        116     140

  Fragments arranged by size: 

                 733     140     137     116     112      84      63      45
                  36      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      35     499    1497 
   Size:         35     464     998

  Fragments arranged by size: 

                 998     464      35
 
BsrBI CCG'CTC

Cuts at:      0    1446    1497 
   Size:       1446      51
 
BsrFI r'CCGG_y

Cuts at:      0     154    1497 
   Size:        154    1343
 
BssHII G'CGCG_C

Cuts at:      0      76    1497 
   Size:         76    1421
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      36      73     657    1497 
   Size:         36      37     584     840

  Fragments arranged by size: 

                 840     584      37      36
 
BstBI TT'CG_AA

Cuts at:      0     783    1497 
   Size:        783     714
 
BstEII G'GTnAC_C

Cuts at:      0     226    1497 
   Size:        226    1271
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1110    1497 
   Size:       1110     387
 
BstYI r'GATC_y

Cuts at:      0     386    1497 
   Size:        386    1111
 
Bsu36I CC'TnA_GG

Cuts at:      0    1399    1497 
   Size:       1399      98
 
BtgI C'CryG_G

Cuts at:      0     596    1184    1251    1295    1497 
   Size:        596     588      67      44     202

  Fragments arranged by size: 

                 596     588     202      67      44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     229    1497 
   Size:        229    1268
 
BtsI GCAGTG_nn'

Cuts at:      0     255     744    1497 
   Size:        255     489     753

  Fragments arranged by size: 

                 753     489     255
 
DraIII CAC_nnn'GTG

Cuts at:      0     903     916    1497 
   Size:        903      13     581

  Fragments arranged by size: 

                 903     581      13
 
EaeI y'GGCC_r

Cuts at:      0     269     593     861     905     918     971    1298    1497 
   Size:        269     324     268      44      13      53     327     199

  Fragments arranged by size: 

                 327     324     269     268     199      53      44      13
            
 
EcoICRI GAG'CTC

Cuts at:      0    1239    1497 
   Size:       1239     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     462    1040    1497 
   Size:         25     437     578     457

  Fragments arranged by size: 

                 578     457     437      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     286    1497 
   Size:        286    1211
 
EcoO109I rG'GnC_Cy

Cuts at:      0     951    1169    1335    1497 
   Size:        951     218     166     162

  Fragments arranged by size: 

                 951     218     166     162
 
HaeII r_GCGC'y

Cuts at:      0     155    1413    1497 
   Size:        155    1258      84

  Fragments arranged by size: 

                1258     155      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48    1497 
   Size:         16      32    1449

  Fragments arranged by size: 

                1449      32      16
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1403    1497 
   Size:       1403      94
 
MscI TGG'CCA

Cuts at:      0     271     863     907     920     973    1300    1497 
   Size:        271     592      44      13      53     327     197

  Fragments arranged by size: 

                 592     327     271     197      53      44      13
 
MslI CAynn'nnrTG

Cuts at:      0     248     875     878    1497 
   Size:        248     627       3     619

  Fragments arranged by size: 

                 627     619     248       3
 
MspA1I CmG'CkG

Cuts at:      0      45    1497 
   Size:         45    1452
 
NaeI GCC'GGC

Cuts at:      0     156    1497 
   Size:        156    1341
 
NcoI C'CATG_G

Cuts at:      0    1251    1295    1497 
   Size:       1251      44     202

  Fragments arranged by size: 

                1251     202      44
 
NgoMIV G'CCGG_C

Cuts at:      0     154    1497 
   Size:        154    1343
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1217    1497 
   Size:       1217     280
 
PfoI T'CCnGG_A

Cuts at:      0     524    1017    1497 
   Size:        524     493     480

  Fragments arranged by size: 

                 524     493     480
 
PpuMI rG'GwC_Cy

Cuts at:      0    1335    1497 
   Size:       1335     162
 
PspOMI G'GGCC_C

Cuts at:      0     950    1497 
   Size:        950     547
 
PstI C_TGCA'G

Cuts at:      0     653    1046    1497 
   Size:        653     393     451

  Fragments arranged by size: 

                 653     451     393
 
PvuII CAG'CTG

Cuts at:      0      45    1497 
   Size:         45    1452
 
SacI G_AGCT'C

Cuts at:      0    1241    1497 
   Size:       1241     256
 
ScaI AGT'ACT

Cuts at:      0     449    1497 
   Size:        449    1048
 
SfcI C'TryA_G

Cuts at:      0     453     649    1042    1497 
   Size:        453     196     393     455

  Fragments arranged by size: 

                 455     453     393     196
 
SmaI CCC'GGG

Cuts at:      0    1333    1497 
   Size:       1333     164
 
StyI C'CwwG_G

Cuts at:      0      95     194    1251    1295    1301    1485    1497 
   Size:         95      99    1057      44       6     184      12

  Fragments arranged by size: 

                1057     184      99      95      44      12       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      69     138    1069    1497 
   Size:         69      69     931     428

  Fragments arranged by size: 

                 931     428      69      69
 
TatI w'GTAC_w

Cuts at:      0     447     588    1497 
   Size:        447     141     909

  Fragments arranged by size: 

                 909     447     141
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     934    1302    1497 
   Size:        934     368     195

  Fragments arranged by size: 

                 934     368     195
 
XmaI C'CCGG_G

Cuts at:      0    1331    1497 
   Size:       1331     166
 

 Enzymes that do cut:

     AcuI     ApaI     AvaI     BanI    BanII     BbsI    BbvCI    BciVI
     BglI Bme1580I     BplI     BpmI   Bpu10I     BsaI    BsaHI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     ScaI     SfcI
     SmaI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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