Restriction map for
pFN21AE1459
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BbrPI BcgI BfrI BglI BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspEI BsrBI BssHII BssSI
Bst1107I BstZ17I CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
FauI FseI FspI HindIII HpaI
KpnI KspI MamI MluI MroI
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SapI SbfI SfiI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XcmI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 92 CGCG CG^CG
AclI 2078 AACGTT AA^CGTT
AflIII 3128 ACATGT A^C[GA][CT]GT
Alw44I 2032 GTGCAC G^TGCAC
AocI 779 CCTCAGG CC^T[ACGT]AGG
ApaLI 2032 GTGCAC G^TGCAC
AseI 2455 ATTAAT AT^TAAT
AsnI 2455 ATTAAT AT^TAAT
Asp700I 2903 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1218 TGGCCA TGG^CCA
BamHI 616 GGATCC G^GATCC
BbvCI 2833 CCTCAGC CCTCAGC(-5/-2)
BciVI 1140 GTATCC GTATCC(6/5)
BclI 1167 TGATCA T^GATCA
BlpI 762 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 762 GCTGAGC GC^T[ACGT]AGC
BsaAI 924 TACGTG [CT]AC^GT[GA]
BseRI 2831 CTCCTC GAGGAG(10/8)
BspDI 1460 ATCGAT AT^CGAT
BspHI 1845 TCATGA T^CATGA
BspLU11I 3128 ACATGT A^CATGT
BsrDI 2048 GCAATG GCAATG(2/0)
BsrFI 69 GCCGGC [GA]^CCGG[CT]
BstAPI 2619 GCATACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1649 TTCGAA TT^CGAA
BstEII 2274 GGTGACC G^GT[ACGT]ACC
BstPI 2274 GGTGACC G^GT[ACGT]ACC
BstUI 92 CGCG CG^CG
BstXI 1255 CCACTAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 779 CCTCAGG CC^T[ACGT]AGG
CelII 762 GCTGAGC GC^T[ACGT]AGC
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
ClaI 1460 ATCGAT AT^CGAT
DraI 1146 TTTAAA TTT^AAA
DrdI 2438 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1218 TGGCCA [CT]^GGCC[GA]
Eco81I 779 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2274 GGTGACC G^GT[ACGT]ACC
EcoRV 2648 GATATC GAT^ATC
FbaI 1167 TGATCA T^GATCA
HgaI 3001 GCGTC GACGC(5/10)
HincII 2537 GTCAAC GT[CT]^[GA]AC
HindII 2537 GTCAAC GT[CT]^[GA]AC
MfeI 2351 CAATTG C^AATTG
MluNI 1218 TGGCCA TGG^CCA
MscI 1218 TGGCCA TGG^CCA
MunI 2351 CAATTG C^AATTG
MvnI 92 CGCG CG^CG
NaeI 69 GCCGGC GCC^GGC
NgoMIV 69 GCCGGC G^CCGGC
NspI 3128 ACATGT [GA]CATG^[CT]
NspV 1649 TTCGAA TT^CGAA
PshBI 2455 ATTAAT AT^TAAT
Psp1406I 2078 AACGTT AA^CGTT
PvuII 2018 CAGCTG CAG^CTG
RcaI 1845 TCATGA T^CATGA
SacI 31 GAGCTC GAGCT^C
ScaI 986 AGTACT AGT^ACT
SfuI 1649 TTCGAA TT^CGAA
SgrAI 68 CGCCGGCG C[GA]^CCGG[CT]G
XbaI 433 TCTAGA T^CTAGA
XmnI 2903 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsmI 2903 GAATGC GAATGC(1/-1)
2907 GCATTC
BspMI 1612 GCAGGT ACCTGC(4/8)
2028 GCAGGT
DraII 783 AGGACCC [GA]G^G[ACGT]CC[CT]
853 GGGTCCC
DsaI 945 CCATGG C^C[GA][CT]GG
2270 CCATGG
EcoNI 788 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2863 CCTAGCTCAGG
EcoO109I 783 AGGACCC [GA]G^G[ACGT]CC[CT]
853 GGGTCCC
MaeII 925 ACGT A^CGT
2079 ACGT
MspA1I 1777 CAGCGG C[AC]G^C[GT]G
2018 CAGCTG
NcoI 945 CCATGG C^CATGG
2270 CCATGG
PpuMI 783 AGGACCC [GA]G^G[AT]CC[CT]
853 GGGTCCC
SexAI 888 ACCTGGT A^CC[AT]GGT
1487 ACCAGGT
SmaI 84 CCCGGG CCC^GGG
1103 CCCGGG
XmaCI 84 CCCGGG C^CCGGG
1103 CCCGGG
XmaI 84 CCCGGG C^CCGGG
1103 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NarI
NciI NdeI NdeII NlaIII NlaIV
NsiI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII