Restriction map for pFN21AE1459
 (Linear) (Six-base) MAPSORT of: KIEE1459.seq  Check: 7655  from: 1  to: 3225



KIEE1459.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2019    3225 
   Size:       2019    1206
 
AclI AA'CG_TT

Cuts at:      0    2079    3225 
   Size:       2079    1146
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     857    1052    1367    2123    2824    3225 
   Size:        857     195     315     756     701     401

  Fragments arranged by size: 

                 857     756     701     401     315     195
 
AflIII A'CryG_T

Cuts at:      0    3128    3225 
   Size:       3128      97
 
AleI CACnn'nnGTG

Cuts at:      0     830    3225 
   Size:        830    2395
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     848    3225 
   Size:        848    2377
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2695    2764    2875    3225 
   Size:       2695      69     111     350

  Fragments arranged by size: 

                2695     350     111      69
 
ApaLI G'TGCA_C

Cuts at:      0    2032    3225 
   Size:       2032    1193
 
ApoI r'AATT_y

Cuts at:      0     415    1565    2476    2571    3225 
   Size:        415    1150     911      95     654

  Fragments arranged by size: 

                1150     911     654     415      95
 
AseI AT'TA_AT

Cuts at:      0    2456    3225 
   Size:       2456     769
 
AvaI C'yCGr_G

Cuts at:      0      84    1103    3183    3225 
   Size:         84    1019    2080      42

  Fragments arranged by size: 

                2080    1019      84      42
 
BamHI G'GATC_C

Cuts at:      0     616    3225 
   Size:        616    2609
 
BanI G'GyrC_C

Cuts at:      0      19      66      72    3225 
   Size:         19      47       6    3153

  Fragments arranged by size: 

                3153      47      19       6
 
BanII G_rGCy'C

Cuts at:      0      35      91     769    1335    1974    3224    3225 
   Size:         35      56     678     566     639    1250       1

  Fragments arranged by size: 

                1250     678     639     566      56      35       1
 
BbeI G_GCGC'C

Cuts at:      0      23      70      76    3225 
   Size:         23      47       6    3149

  Fragments arranged by size: 

                3149      47      23       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     470    1516    2129    2390    2440    3225 
   Size:        470    1046     613     261      50     785

  Fragments arranged by size: 

                1046     785     613     470     261      50
 
BbvCI CC'TCA_GC

Cuts at:      0    2834    3225 
   Size:       2834     391
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1151    3225 
   Size:       1151    2074
 
BclI T'GATC_A

Cuts at:      0    1167    3225 
   Size:       1167    2058
 
BfrBI ATG'CAT

Cuts at:      0     535    1480    2266    2907    3225 
   Size:        535     945     786     641     318

  Fragments arranged by size: 

                 945     786     641     535     318
 
BglII A'GATC_T

Cuts at:      0     102    2674    3211    3225 
   Size:        102    2572     537      14

  Fragments arranged by size: 

                2572     537     102      14
 
BlpI GC'TnA_GC

Cuts at:      0     763    3225 
   Size:        763    2462
 
Bme1580I G_kGCm'C

Cuts at:      0     325    2036    3225 
   Size:        325    1711    1189

  Fragments arranged by size: 

                1711    1189     325
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1388    1514    1718    3225 
   Size:       1388     126     204    1507

  Fragments arranged by size: 

                1507    1388     204     126
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     460    3225 
   Size:        460    2765
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     629     680     813    1221    2672    2876    3225 
   Size:        629      51     133     408    1451     204     349

  Fragments arranged by size: 

                1451     629     408     349     204     133      51
 
Bpu10I CC'TnA_GC

Cuts at:      0    2834    2868    3225 
   Size:       2834      34     357

  Fragments arranged by size: 

                2834     357      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     478    1772    2912    3225 
   Size:        127     351    1294    1140     313

  Fragments arranged by size: 

                1294    1140     351     313     127
 
BsaAI yAC'GTr

Cuts at:      0     926    3225 
   Size:        926    2299
 
BsaHI Gr'CG_yC

Cuts at:      0      20      67      73    3225 
   Size:         20      47       6    3152

  Fragments arranged by size: 

                3152      47      20       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3146    3176    3225 
   Size:       3146      30      49

  Fragments arranged by size: 

                3146      49      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2822    3225 
   Size:       2822     403
 
BseYI C'CCAG_C

Cuts at:      0     850    1775    2007    2781    3225 
   Size:        850     925     232     774     444

  Fragments arranged by size: 

                 925     850     774     444     232
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1631    2047    2945    3225 
   Size:       1631     416     898     280

  Fragments arranged by size: 

                1631     898     416     280
 
BsiHKAI G_wGCw'C

Cuts at:      0      35    1774    2036    3225 
   Size:         35    1739     262    1189

  Fragments arranged by size: 

                1739    1189     262      35
 
BsmI GAATG_Cn'

Cuts at:      0    2907    2909    3225 
   Size:       2907       2     316

  Fragments arranged by size: 

                2907     316       2
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     209    2726    2894    3225 
   Size:        209    2517     168     331

  Fragments arranged by size: 

                2517     331     209     168
 
Bsp1286I G_dGCh'C

Cuts at:      0      35      91     325     769    1335    1774    1974    2036 
   Size:         35      56     234     444     566     439     200      62

Cuts at:   2036    3224    3225 
   Size:       1188       1

  Fragments arranged by size: 

                1188     566     444     439     234     200      62      56
                  35       1
 
BspHI T'CATG_A

Cuts at:      0    1845    3225 
   Size:       1845    1380
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1603    2019    3225 
   Size:       1603     416    1206

  Fragments arranged by size: 

                1603    1206     416
 
BsrDI GCAATG_nn'

Cuts at:      0    2055    3225 
   Size:       2055    1170
 
BsrFI r'CCGG_y

Cuts at:      0      69    3225 
   Size:         69    3156
 
BsrGI T'GTAC_A

Cuts at:      0    1707    2355    2429    3225 
   Size:       1707     648      74     796

  Fragments arranged by size: 

                1707     796     648      74
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2625    3225 
   Size:       2625     600
 
BstBI TT'CG_AA

Cuts at:      0    1650    3225 
   Size:       1650    1575
 
BstEII G'GTnAC_C

Cuts at:      0    2274    3225 
   Size:       2274     951
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1262    3225 
   Size:       1262    1963
 
BstYI r'GATC_y

Cuts at:      0     102     147     616     736    1132    1864    2674    3211 
   Size:        102      45     469     120     396     732     810     537

Cuts at:   3211    3225 
   Size:         14

  Fragments arranged by size: 

                 810     732     537     469     396     120     102      45
                  14
 
Bsu36I CC'TnA_GG

Cuts at:      0     780    3225 
   Size:        780    2445
 
BtgI C'CryG_G

Cuts at:      0     945    2270    3225 
   Size:        945    1325     955

  Fragments arranged by size: 

                1325     955     945
 
BtsI GCAGTG_nn'

Cuts at:      0    2030    2308    3225 
   Size:       2030     278     917

  Fragments arranged by size: 

                2030     917     278
 
ClaI AT'CG_AT

Cuts at:      0    1461    3225 
   Size:       1461    1764
 
DraI TTT'AAA

Cuts at:      0    1148    3225 
   Size:       1148    2077
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2444    3225 
   Size:       2444     781
 
EaeI y'GGCC_r

Cuts at:      0    1218    3225 
   Size:       1218    2007
 
EarI CTCTTCn'nnn_

Cuts at:      0     216     516    2117    3225 
   Size:        216     300    1601    1108

  Fragments arranged by size: 

                1601    1108     300     216
 
EcoICRI GAG'CTC

Cuts at:      0      33    3225 
   Size:         33    3192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     629     680     813     857    1052    1221    1367    2123 
   Size:        629      51     133      44     195     169     146     756

Cuts at:   2123    2672    2824    2876    3225 
   Size:        549     152      52     349

  Fragments arranged by size: 

                 756     629     549     349     195     169     152     146
                 133      52      51      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     792    2867    3225 
   Size:        792    2075     358

  Fragments arranged by size: 

                2075     792     358
 
EcoO109I rG'GnC_Cy

Cuts at:      0     784     854    3225 
   Size:        784      70    2371

  Fragments arranged by size: 

                2371     784      70
 
EcoRV GAT'ATC

Cuts at:      0    2650    3225 
   Size:       2650     575
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1043    3225 
   Size:       1043    2182
 
HaeII r_GCGC'y

Cuts at:      0      23      70      76    3225 
   Size:         23      47       6    3149

  Fragments arranged by size: 

                3149      47      23       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141     562     594     728     760    2331    2363 
   Size:        109      32     421      32     134      32    1571      32

Cuts at:   2363    2694    2726    2986    3018    3225 
   Size:        331      32     260      32     207

  Fragments arranged by size: 

                1571     421     331     260     207     134     109      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2539    3225 
   Size:       2539     686
 
KasI G'GCGC_C

Cuts at:      0      19      66      72    3225 
   Size:         19      47       6    3153

  Fragments arranged by size: 

                3153      47      19       6
 
MfeI C'AATT_G

Cuts at:      0    2351    3225 
   Size:       2351     874
 
MscI TGG'CCA

Cuts at:      0    1220    3225 
   Size:       1220    2005
 
MslI CAynn'nnrTG

Cuts at:      0     383     563     830    2213    2902    3225 
   Size:        383     180     267    1383     689     323

  Fragments arranged by size: 

                1383     689     383     323     267     180
 
MspA1I CmG'CkG

Cuts at:      0    1779    2020    3225 
   Size:       1779     241    1205

  Fragments arranged by size: 

                1779    1205     241
 
NaeI GCC'GGC

Cuts at:      0      71    3225 
   Size:         71    3154
 
NarI GG'CG_CC

Cuts at:      0      20      67      73    3225 
   Size:         20      47       6    3152

  Fragments arranged by size: 

                3152      47      20       6
 
NcoI C'CATG_G

Cuts at:      0     945    2270    3225 
   Size:        945    1325     955

  Fragments arranged by size: 

                1325     955     945
 
NdeI CA'TA_TG

Cuts at:      0    2262    2336    2742    3225 
   Size:       2262      74     406     483

  Fragments arranged by size: 

                2262     483     406      74
 
NgoMIV G'CCGG_C

Cuts at:      0      69    3225 
   Size:         69    3156
 
NsiI A_TGCA'T

Cuts at:      0     537    1482    2268    2909    3225 
   Size:        537     945     786     641     316

  Fragments arranged by size: 

                 945     786     641     537     316
 
NspI r_CATG'y

Cuts at:      0    3132    3225 
   Size:       3132      93
 
PasI CC'CwG_GG

Cuts at:      0     902    3225 
   Size:        902    2323
 
PciI A'CATG_T

Cuts at:      0    3128    3225 
   Size:       3128      97
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     193     301    1216    3016    3225 
   Size:        193     108     915    1800     209

  Fragments arranged by size: 

                1800     915     209     193     108
 
PpuMI rG'GwC_Cy

Cuts at:      0     784     854    3225 
   Size:        784      70    2371

  Fragments arranged by size: 

                2371     784      70
 
PstI C_TGCA'G

Cuts at:      0     102     600     972    1748    2025    3086    3225 
   Size:        102     498     372     776     277    1061     139

  Fragments arranged by size: 

                1061     776     498     372     277     139     102
 
PvuII CAG'CTG

Cuts at:      0    2020    3225 
   Size:       2020    1205
 
SacI G_AGCT'C

Cuts at:      0      35    3225 
   Size:         35    3190
 
SanDI GG'GwC_CC

Cuts at:      0     854    3225 
   Size:        854    2371
 
ScaI AGT'ACT

Cuts at:      0     988    3225 
   Size:        988    2237
 
SexAI A'CCwGG_T

Cuts at:      0     888    1487    3225 
   Size:        888     599    1738

  Fragments arranged by size: 

                1738     888     599
 
SfcI C'TryA_G

Cuts at:      0      98     391     596     968    1744    2021    3082    3225 
   Size:         98     293     205     372     776     277    1061     143

  Fragments arranged by size: 

                1061     776     372     293     277     205     143      98
            
 
SfoI GGC'GCC

Cuts at:      0      21      68      74    3225 
   Size:         21      47       6    3151

  Fragments arranged by size: 

                3151      47      21       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0      69    3225 
   Size:         69    3156
 
SmaI CCC'GGG

Cuts at:      0      86    1105    3225 
   Size:         86    1019    2120

  Fragments arranged by size: 

                2120    1019      86
 
SmlI C'TyrA_G

Cuts at:      0     106     457    1787    2927    3225 
   Size:        106     351    1330    1140     298

  Fragments arranged by size: 

                1330    1140     351     298     106
 
StuI AGG'CCT

Cuts at:      0     429     959    2184    3225 
   Size:        429     530    1225    1041

  Fragments arranged by size: 

                1225    1041     530     429
 
StyI C'CwwG_G

Cuts at:      0     612     945     960    1083    1128    2270    2798    3150 
   Size:        612     333      15     123      45    1142     528     352

Cuts at:   3150    3225 
   Size:         75

  Fragments arranged by size: 

                1142     612     528     352     333     123      75      45
                  15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     723    3225 
   Size:        723    2502
 
TatI w'GTAC_w

Cuts at:      0     986    1549    1707    1811    2217    2355    2429    3225 
   Size:        986     563     158     104     406     138      74     796

  Fragments arranged by size: 

                 986     796     563     406     158     138     104      74
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3146    3178    3225 
   Size:       3146      32      47

  Fragments arranged by size: 

                3146      47      32
 
XbaI T'CTAG_A

Cuts at:      0     433    3225 
   Size:        433    2792
 
XmaI C'CCGG_G

Cuts at:      0      84    1103    3225 
   Size:         84    1019    2122

  Fragments arranged by size: 

                2122    1019      84
 
XmnI GAAnn'nnTTC

Cuts at:      0    2907    3225 
   Size:       2907     318
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI     ClaI     DraI     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I
   HincII     KasI     MfeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI
    PpuMI     PstI    PvuII     SacI    SanDI     ScaI    SexAI     SfcI
     SfoI    SgrAI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI     AloI
     ApaI     AscI    AsiSI    AvrII     BaeI     BcgI     BglI    BmgBI
     BmtI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BsrBI
   BssHII    BssSI  BstZ17I    BtgZI    CspCI   DraIII     EagI     EciI
    EcoRI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MluI
     MmeI     NheI     NotI     NruI     PacI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SapI     SbfI     SfiI     SgfI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com