################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI KasI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp509I TspRI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 167 GGTACC G^GTACC Asp718I 167 GGTACC G^GTACC AvaI 247 CCCGGG C^[CT]CG[GA]G BanI 167 GGTACC G^G[CT][GA]CC BbsI 7 GAAGAC GAAGAC(2/6) BfaI 80 CTAG C^TAG BpuAI 7 GAAGAC GAAGAC(2/6) BsaI 16 GAGACC GGTCTC(1/5) BsiYI 197 CCACATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 197 CCACATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 16 GAGAC GTCTC(1/5) BsmFI 186 GTCCC GGGAC(10/14) BsoBI 247 CCCGGG C^[CT]CG[GA]G BsrDI 127 GCAATG GCAATG(2/0) Cac8I 89 GCTGGC GC[ACGT]^[ACGT]GC DpnII 255 GATC ^GATC EcoRV 148 GATATC GAT^ATC Fnu4HI 56 GCCGC GC^[ACGT]GC HapII 248 CCGG C^CGG HpaII 248 CCGG C^CGG ItaI 56 GCCGC GC^[ACGT]GC KpnI 167 GGTACC GGTAC^C MaeI 80 CTAG C^TAG MaeII 241 ACGT A^CGT MaeIII 208 GTCAC ^GT[ACGT]AC MboI 255 GATC ^GATC MspA1I 114 CCGCTG C[AC]G^C[GT]G MspI 248 CCGG C^CGG MwoI 127 GCAATGACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 255 GATC ^GATC NlaIII 12 CATG CATG^ NlaIV 167 GGTACC GG[ACGT]^[ACGT]CC PflMI 197 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 255 GATC ^GATC SmaI 247 CCCGGG CCC^GGG Tsp45I 208 GTCAC ^GT[GC]AC Van91I 197 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 247 CCCGGG C^CCGGG XmaI 247 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 168 GTAC GT^AC 180 GTAC BcnI 247 CCCGG CC^[GC]GG 248 CCGGG BsaJI 203 CCTGGG C^C[ACGT][ACGT]GG 247 CCCGGG BsrI 215 ACTGG ACTGG(1/-1) 183 CCAGT BstF5I 200 CATCC GGATG(2/0) 219 GGATG BstNI 102 CCTGG CC^[AT]GG 203 CCTGG EcoRII 102 CCTGG ^CC[AT]GG 203 CCTGG FokI 200 CATCC GGATG(9/13) 219 GGATG MboII 7 GAAGA GAAGA(8/7) 252 GAAGA MnlI 189 CCTC CCTC(7/6) 143 GAGG MvaI 102 CCTGG CC^[AT]GG 203 CCTGG NciI 247 CCCGG CC^[GC]GG 248 CCGGG RsaI 168 GTAC GT^AC 180 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AciI BssKI ScrFI