################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AfaI 30 GTAC GT^AC AvaI 452 CCCGAG C^[CT]CG[GA]G AvaII 68 GGACC G^G[AT]CC BalI 272 TGGCCA TGG^CCA BanI 393 GGCACC G^G[CT][GA]CC BcnI 43 CCCGG CC^[GC]GG BglII 35 AGATCT A^GATCT BlpI 352 GCTAAGC GC^T[ACGT]AGC Bpu1102I 352 GCTAAGC GC^T[ACGT]AGC BsgI 15 GTGCAG GTGCAG(16/14) BsmFI 183 GGGAC GGGAC(10/14) BsoBI 452 CCCGAG C^[CT]CG[GA]G BsrDI 376 GCAATG GCAATG(2/0) BstNI 65 CCAGG CC^[AT]GG BstYI 35 AGATCT [GA]^GATC[CT] Cac8I 357 GCAAGC GC[ACGT]^[ACGT]GC CelII 352 GCTAAGC GC^T[ACGT]AGC CfoI 57 GCGC GCG^C DpnII 36 GATC ^GATC EaeI 272 TGGCCA [CT]^GGCC[GA] EcoRII 65 CCAGG ^CC[AT]GG HaeII 56 GGCGCT [GA]GCGC^[CT] HapII 44 CCGG C^CGG HhaI 57 GCGC GCG^C HinP1I 57 GCGC G^CGC HpaII 44 CCGG C^CGG MboI 36 GATC ^GATC MboII 193 GAAGA GAAGA(8/7) MflI 35 AGATCT [GA]^GATC[CT] MluNI 272 TGGCCA TGG^CCA MscI 272 TGGCCA TGG^CCA MseI 95 TTAA T^TAA MspI 44 CCGG C^CGG MvaI 65 CCAGG CC^[AT]GG NciI 43 CCCGG CC^[GC]GG NdeII 36 GATC ^GATC NlaIV 393 GGCACC GG[ACGT]^[ACGT]CC PleI 109 GAGTC GAGTC(4/5) RsaI 30 GTAC GT^AC Sau3AI 36 GATC ^GATC SfcI 162 CTACAG C^T[GA][CT]AG TaqI 8 TCGA T^CGA Tru9I 95 TTAA T^TAA TthHB8I 8 TCGA T^CGA XhoII 35 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 216 CCGC CCGC(-3/-1) 26 GCGG AcsI 197 AAATTC [GA]^AATT[CT] 229 AAATTC ApoI 197 AAATTC [GA]^AATT[CT] 229 AAATTC Asp700I 78 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 193 GAAGAAATTC BsaJI 42 CCCCGG C^C[ACGT][ACGT]GG 136 CCTCGG BssKI 43 CCCGG ^CC[ACGT]GG 65 CCAGG BstAPI 253 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 376 GCAATGGCTGC Cfr13I 68 GGACC G^G[ACGT]CC 239 GGGCC FauI 215 CCCGC CCCGC(4/6) 26 GCGGG HaeIII 240 GGCC GG^CC 273 GGCC Sau96I 68 GGACC G^G[ACGT]CC 239 GGGCC ScrFI 43 CCCGG CC^[ACGT]GG 65 CCAGG SfaNI 253 GCATC GCATC(5/9) 361 GCATC TfiI 132 GAATC G^A[AT]TC 282 GAATC Tsp509I 198 AATT ^AATT 230 AATT XmnI 78 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 193 GAAGAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BbvI BsiYI BslI BsrI DdeI Fnu4HI HinfI ItaI MnlI MwoI TseI