################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 681 GTCTAC GT^[AC][GT]AC AclI 1079 AACGTT AA^CGTT AcsI 424 GAATTT [GA]^AATT[CT] AcyI 588 GGCGTC G[GA]^CG[CT]C AlwNI 450 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 424 GAATTT [GA]^AATT[CT] AspI 517 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 35 CTCGGG C^[CT]CG[GA]G BamHI 156 GGATCC G^GATCC BanI 476 GGCACC G^G[CT][GA]CC BbsI 321 GAAGAC GAAGAC(2/6) BfaI 511 CTAG C^TAG BglI 1010 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1005 CTGGAG CTGGAG(16/14) BpuAI 321 GAAGAC GAAGAC(2/6) BsaHI 588 GGCGTC G[GA]^CG[CT]C BsaI 829 GAGACC GGTCTC(1/5) BsmBI 590 CGTCTC CGTCTC(1/5) BsmI 647 GCATTC GAATGC(1/-1) BsoBI 35 CTCGGG C^[CT]CG[GA]G BspHI 1536 TCATGA T^CATGA BsrFI 569 ACCGGC [GA]^CCGG[CT] BstAPI 567 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 867 GGTGACC G^GT[ACGT]ACC BstPI 867 GGTGACC G^GT[ACGT]ACC CfoI 987 GCGC GCG^C Cfr10I 569 ACCGGC [GA]^CCGG[CT] EcoNI 1588 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 867 GGTGACC G^GT[ACGT]ACC EcoT22I 237 ATGCAT ATGCA^T HaeII 986 GGCGCT [GA]GCGC^[CT] HgaI 589 GCGTC GACGC(5/10) HhaI 987 GCGC GCG^C Hin1I 588 GGCGTC G[GA]^CG[CT]C HinP1I 987 GCGC G^CGC MaeI 511 CTAG C^TAG NsiI 237 ATGCAT ATGCA^T PflFI 517 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 831 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1079 AACGTT AA^CGTT PstI 574 CTGCAG CTGCA^G RcaI 1536 TCATGA T^CATGA SacI 1318 GAGCTC GAGCT^C SfcI 574 CTGCAG C^T[GA][CT]AG Tth111I 517 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AfaI 1261 GTAC GT^AC 1452 GTAC BglII 411 AGATCT A^GATCT 1137 AGATCT BspMI 875 ACCTGC ACCTGC(4/8) 1541 ACCTGC HinfI 517 GACTC G^A[ACGT]TC 935 GAGTC PflMI 1202 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1486 CCAGTGCTTGG PleI 517 GACTC GAGTC(4/5) 935 GAGTC PvuII 450 CAGCTG CAG^CTG 923 CAGCTG RsaI 1261 GTAC GT^AC 1452 GTAC Van91I 1202 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1486 CCAGTGCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AluI AlwI ApaI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII