Restriction map for pFN21AE4514
 (Linear) (Six-base) MAPSORT of: KIEE4514.seq  Check: 3265  from: 1  to: 1638



KIEE4514.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:14  ..

AccI GT'mk_AC

Cuts at:      0     682    1638 
   Size:        682     956
 
AclI AA'CG_TT

Cuts at:      0    1080    1638 
   Size:       1080     558
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     905    1638 
   Size:        583     322     733

  Fragments arranged by size: 

                 733     583     322
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1042    1638 
   Size:       1042     596
 
AlwNI CAG_nnn'CTG

Cuts at:      0     455    1638 
   Size:        455    1183
 
ApaI G_GGCC'C

Cuts at:      0     342     759    1404    1503    1638 
   Size:        342     417     645      99     135

  Fragments arranged by size: 

                 645     417     342     135      99
 
ApoI r'AATT_y

Cuts at:      0     424    1638 
   Size:        424    1214
 
AvaI C'yCGr_G

Cuts at:      0      35    1638 
   Size:         35    1603
 
BamHI G'GATC_C

Cuts at:      0     156    1638 
   Size:        156    1482
 
BanI G'GyrC_C

Cuts at:      0     476    1638 
   Size:        476    1162
 
BanII G_rGCy'C

Cuts at:      0     100     342     759    1012    1322    1359    1404    1503 
   Size:        100     242     417     253     310      37      45      99

Cuts at:   1503    1638 
   Size:        135

  Fragments arranged by size: 

                 417     310     253     242     135     100      99      45
                  37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     328    1638 
   Size:        328    1310
 
BfrBI ATG'CAT

Cuts at:      0     239    1638 
   Size:        239    1399
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1016    1638 
   Size:       1016     622
 
BglII A'GATC_T

Cuts at:      0     411    1137    1638 
   Size:        411     726     501

  Fragments arranged by size: 

                 726     501     411
 
Bme1580I G_kGCm'C

Cuts at:      0     342     708     759    1404    1503    1638 
   Size:        342     366      51     645      99     135

  Fragments arranged by size: 

                 645     366     342     135      99      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     755    1211    1461    1480    1638 
   Size:        755     456     250      19     158

  Fragments arranged by size: 

                 755     456     250     158      19
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1215    1638 
   Size:       1215     423
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1026    1638 
   Size:       1026     612
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     823    1638 
   Size:        823     815
 
BsaHI Gr'CG_yC

Cuts at:      0     589    1638 
   Size:        589    1049
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     211     241     658     688     851     881    1638 
   Size:        211      30     417      30     163      30     757

  Fragments arranged by size: 

                 757     417     211     163      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      14     872    1189    1638 
   Size:         14     858     317     449

  Fragments arranged by size: 

                 858     449     317      14
 
BseYI C'CCAG_C

Cuts at:      0     335    1358    1364    1516    1638 
   Size:        335    1023       6     152     122

  Fragments arranged by size: 

                1023     335     152     122       6
 
BsiHKAI G_wGCw'C

Cuts at:      0       7      62    1322    1638 
   Size:          7      55    1260     316

  Fragments arranged by size: 

                1260     316      55       7
 
BsmI GAATG_Cn'

Cuts at:      0     647    1638 
   Size:        647     991
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     596    1638 
   Size:        596    1042
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      62     100     342     708     759    1012    1322 
   Size:          7      55      38     242     366      51     253     310

Cuts at:   1322    1359    1404    1503    1638 
   Size:         37      45      99     135

  Fragments arranged by size: 

                 366     310     253     242     135      99      55      51
                  45      38      37       7
 
BspHI T'CATG_A

Cuts at:      0    1536    1638 
   Size:       1536     102
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     884    1550    1638 
   Size:        884     666      88

  Fragments arranged by size: 

                 884     666      88
 
BsrFI r'CCGG_y

Cuts at:      0     569    1638 
   Size:        569    1069
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     573    1638 
   Size:        573    1065
 
BstEII G'GTnAC_C

Cuts at:      0     867    1638 
   Size:        867     771
 
BstYI r'GATC_y

Cuts at:      0     156     411    1137    1638 
   Size:        156     255     726     501

  Fragments arranged by size: 

                 726     501     255     156
 
BtgI C'CryG_G

Cuts at:      0     186     372     791    1202    1638 
   Size:        186     186     419     411     436

  Fragments arranged by size: 

                 436     419     411     186     186
 
BtsI GCAGTG_nn'

Cuts at:      0     506    1638 
   Size:        506    1132
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1112    1147    1638 
   Size:       1112      35     491

  Fragments arranged by size: 

                1112     491      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     296     369     699    1638 
   Size:        226      70      73     330     939

  Fragments arranged by size: 

                 939     330     226      73      70
 
EcoICRI GAG'CTC

Cuts at:      0    1320    1638 
   Size:       1320     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     905    1026    1638 
   Size:        583     322     121     612

  Fragments arranged by size: 

                 612     583     322     121
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1592    1638 
   Size:       1592      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     377     756    1212    1400    1401    1584    1638 
   Size:        339      38     379     456     188       1     183      54

  Fragments arranged by size: 

                 456     379     339     188     183      54      38       1
            
 
HaeII r_GCGC'y

Cuts at:      0     990    1638 
   Size:        990     648
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     141     173     396     428     658     690    1638 
   Size:        141      32     223      32     230      32     948

  Fragments arranged by size: 

                 948     230     223     141      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1086    1638 
   Size:       1086     552
 
MspA1I CmG'CkG

Cuts at:      0     452     925    1449    1638 
   Size:        452     473     524     189

  Fragments arranged by size: 

                 524     473     452     189
 
NcoI C'CATG_G

Cuts at:      0     186     372    1202    1638 
   Size:        186     186     830     436

  Fragments arranged by size: 

                 830     436     186     186
 
NsiI A_TGCA'T

Cuts at:      0     241    1638 
   Size:        241    1397
 
PasI CC'CwG_GG

Cuts at:      0     724    1594    1638 
   Size:        724     870      44

  Fragments arranged by size: 

                 870     724      44
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1208    1492    1638 
   Size:       1208     284     146

  Fragments arranged by size: 

                1208     284     146
 
PpuMI rG'GwC_Cy

Cuts at:      0     377    1212    1584    1638 
   Size:        377     835     372      54

  Fragments arranged by size: 

                 835     377     372      54
 
PshAI GACnn'nnGTC

Cuts at:      0     835    1638 
   Size:        835     803
 
PspOMI G'GGCC_C

Cuts at:      0     338     755    1400    1499    1638 
   Size:        338     417     645      99     139

  Fragments arranged by size: 

                 645     417     338     139      99
 
PstI C_TGCA'G

Cuts at:      0     578    1638 
   Size:        578    1060
 
PvuII CAG'CTG

Cuts at:      0     452     925    1638 
   Size:        452     473     713

  Fragments arranged by size: 

                 713     473     452
 
SacI G_AGCT'C

Cuts at:      0    1322    1638 
   Size:       1322     316
 
SanDI GG'GwC_CC

Cuts at:      0     377    1212    1584    1638 
   Size:        377     835     372      54

  Fragments arranged by size: 

                 835     377     372      54
 
SfcI C'TryA_G

Cuts at:      0     574    1638 
   Size:        574    1064
 
StyI C'CwwG_G

Cuts at:      0     186     372    1202    1255    1579    1638 
   Size:        186     186     830      53     324      59

  Fragments arranged by size: 

                 830     324     186     186      59      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     306    1638 
   Size:        306    1332
 
TatI w'GTAC_w

Cuts at:      0    1451    1638 
   Size:       1451     187
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     211     243     851     883    1365    1397    1638 
   Size:        211      32     608      32     482      32     241

  Fragments arranged by size: 

                 608     482     241     211      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     520    1638 
   Size:        520    1118
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI    BfrBI     BglI    BglII Bme1580I
     BmrI     BplI     BpmI     BsaI    BsaHI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI   BstAPI
   BstEII    BstYI     BtgI     BtsI    CspCI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     MmeI   MspA1I     NcoI     NsiI
     PasI    PflMI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
    SanDI     SfcI     StyI    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI     BlpI    BmgBI     BmtI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI
    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com