(Linear) (Six-base) MAPSORT of: KIEE4514.seq Check: 3265 from: 1 to: 1638 KIEE4514.orf With 182 enzymes: SgfI * March 22, 2012 18:14 .. AccI GT'mk_AC Cuts at: 0 682 1638 Size: 682 956 AclI AA'CG_TT Cuts at: 0 1080 1638 Size: 1080 558 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 905 1638 Size: 583 322 733 Fragments arranged by size: 733 583 322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1042 1638 Size: 1042 596 AlwNI CAG_nnn'CTG Cuts at: 0 455 1638 Size: 455 1183 ApaI G_GGCC'C Cuts at: 0 342 759 1404 1503 1638 Size: 342 417 645 99 135 Fragments arranged by size: 645 417 342 135 99 ApoI r'AATT_y Cuts at: 0 424 1638 Size: 424 1214 AvaI C'yCGr_G Cuts at: 0 35 1638 Size: 35 1603 BamHI G'GATC_C Cuts at: 0 156 1638 Size: 156 1482 BanI G'GyrC_C Cuts at: 0 476 1638 Size: 476 1162 BanII G_rGCy'C Cuts at: 0 100 342 759 1012 1322 1359 1404 1503 Size: 100 242 417 253 310 37 45 99 Cuts at: 1503 1638 Size: 135 Fragments arranged by size: 417 310 253 242 135 100 99 45 37 BbsI GAAGACnn'nnnn_ Cuts at: 0 328 1638 Size: 328 1310 BfrBI ATG'CAT Cuts at: 0 239 1638 Size: 239 1399 BglI GCCn_nnn'nGGC Cuts at: 0 1016 1638 Size: 1016 622 BglII A'GATC_T Cuts at: 0 411 1137 1638 Size: 411 726 501 Fragments arranged by size: 726 501 411 Bme1580I G_kGCm'C Cuts at: 0 342 708 759 1404 1503 1638 Size: 342 366 51 645 99 135 Fragments arranged by size: 645 366 342 135 99 51 BmrI ACTGGGnnnn_n' Cuts at: 0 755 1211 1461 1480 1638 Size: 755 456 250 19 158 Fragments arranged by size: 755 456 250 158 19 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1215 1638 Size: 1215 423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1026 1638 Size: 1026 612 BsaI GGTCTCn'nnnn_ Cuts at: 0 823 1638 Size: 823 815 BsaHI Gr'CG_yC Cuts at: 0 589 1638 Size: 589 1049 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 211 241 658 688 851 881 1638 Size: 211 30 417 30 163 30 757 Fragments arranged by size: 757 417 211 163 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 14 872 1189 1638 Size: 14 858 317 449 Fragments arranged by size: 858 449 317 14 BseYI C'CCAG_C Cuts at: 0 335 1358 1364 1516 1638 Size: 335 1023 6 152 122 Fragments arranged by size: 1023 335 152 122 6 BsiHKAI G_wGCw'C Cuts at: 0 7 62 1322 1638 Size: 7 55 1260 316 Fragments arranged by size: 1260 316 55 7 BsmI GAATG_Cn' Cuts at: 0 647 1638 Size: 647 991 BsmBI CGTCTCn'nnnn_ Cuts at: 0 596 1638 Size: 596 1042 Bsp1286I G_dGCh'C Cuts at: 0 7 62 100 342 708 759 1012 1322 Size: 7 55 38 242 366 51 253 310 Cuts at: 1322 1359 1404 1503 1638 Size: 37 45 99 135 Fragments arranged by size: 366 310 253 242 135 99 55 51 45 38 37 7 BspHI T'CATG_A Cuts at: 0 1536 1638 Size: 1536 102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 884 1550 1638 Size: 884 666 88 Fragments arranged by size: 884 666 88 BsrFI r'CCGG_y Cuts at: 0 569 1638 Size: 569 1069 BstAPI GCAn_nnn'nTGC Cuts at: 0 573 1638 Size: 573 1065 BstEII G'GTnAC_C Cuts at: 0 867 1638 Size: 867 771 BstYI r'GATC_y Cuts at: 0 156 411 1137 1638 Size: 156 255 726 501 Fragments arranged by size: 726 501 255 156 BtgI C'CryG_G Cuts at: 0 186 372 791 1202 1638 Size: 186 186 419 411 436 Fragments arranged by size: 436 419 411 186 186 BtsI GCAGTG_nn' Cuts at: 0 506 1638 Size: 506 1132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1112 1147 1638 Size: 1112 35 491 Fragments arranged by size: 1112 491 35 EarI CTCTTCn'nnn_ Cuts at: 0 226 296 369 699 1638 Size: 226 70 73 330 939 Fragments arranged by size: 939 330 226 73 70 EcoICRI GAG'CTC Cuts at: 0 1320 1638 Size: 1320 318 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 905 1026 1638 Size: 583 322 121 612 Fragments arranged by size: 612 583 322 121 EcoNI CCTnn'n_nnAGG Cuts at: 0 1592 1638 Size: 1592 46 EcoO109I rG'GnC_Cy Cuts at: 0 339 377 756 1212 1400 1401 1584 1638 Size: 339 38 379 456 188 1 183 54 Fragments arranged by size: 456 379 339 188 183 54 38 1 HaeII r_GCGC'y Cuts at: 0 990 1638 Size: 990 648 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 141 173 396 428 658 690 1638 Size: 141 32 223 32 230 32 948 Fragments arranged by size: 948 230 223 141 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1086 1638 Size: 1086 552 MspA1I CmG'CkG Cuts at: 0 452 925 1449 1638 Size: 452 473 524 189 Fragments arranged by size: 524 473 452 189 NcoI C'CATG_G Cuts at: 0 186 372 1202 1638 Size: 186 186 830 436 Fragments arranged by size: 830 436 186 186 NsiI A_TGCA'T Cuts at: 0 241 1638 Size: 241 1397 PasI CC'CwG_GG Cuts at: 0 724 1594 1638 Size: 724 870 44 Fragments arranged by size: 870 724 44 PflMI CCAn_nnn'nTGG Cuts at: 0 1208 1492 1638 Size: 1208 284 146 Fragments arranged by size: 1208 284 146 PpuMI rG'GwC_Cy Cuts at: 0 377 1212 1584 1638 Size: 377 835 372 54 Fragments arranged by size: 835 377 372 54 PshAI GACnn'nnGTC Cuts at: 0 835 1638 Size: 835 803 PspOMI G'GGCC_C Cuts at: 0 338 755 1400 1499 1638 Size: 338 417 645 99 139 Fragments arranged by size: 645 417 338 139 99 PstI C_TGCA'G Cuts at: 0 578 1638 Size: 578 1060 PvuII CAG'CTG Cuts at: 0 452 925 1638 Size: 452 473 713 Fragments arranged by size: 713 473 452 SacI G_AGCT'C Cuts at: 0 1322 1638 Size: 1322 316 SanDI GG'GwC_CC Cuts at: 0 377 1212 1584 1638 Size: 377 835 372 54 Fragments arranged by size: 835 377 372 54 SfcI C'TryA_G Cuts at: 0 574 1638 Size: 574 1064 StyI C'CwwG_G Cuts at: 0 186 372 1202 1255 1579 1638 Size: 186 186 830 53 324 59 Fragments arranged by size: 830 324 186 186 59 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 306 1638 Size: 306 1332 TatI w'GTAC_w Cuts at: 0 1451 1638 Size: 1451 187 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 211 243 851 883 1365 1397 1638 Size: 211 32 608 32 482 32 241 Fragments arranged by size: 608 482 241 211 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 520 1638 Size: 520 1118 Enzymes that do cut: AccI AclI AcuI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BfrBI BglI BglII Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BstAPI BstEII BstYI BtgI BtsI CspCI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MspA1I NcoI NsiI PasI PflMI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SfcI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI