(Circular) (Six-base) MAPSORT of: pFN21AE6217.seq Check: 6424 from: 1 to: 5497 pFN21AE6217 5497 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2361 3307 278 Size: 53 83 186 936 825 946 2468 Fragments arranged by size: 2468 946 936 825 186 83 53 AccI GT'mk_AC Cuts at: 1937 2391 2840 1937 Size: 454 449 4594 Fragments arranged by size: 4594 454 449 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3560 4944 5212 1632 Size: 1928 1384 268 1917 Fragments arranged by size: 1928 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 2520 4396 5373 1203 Size: 1317 1876 977 1327 Fragments arranged by size: 1876 1327 1317 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 2718 3240 4812 2718 Size: 522 1572 3403 Fragments arranged by size: 3403 1572 522 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2699 2806 3032 3184 5293 1493 Size: 1206 107 226 152 2109 1697 Fragments arranged by size: 2109 1697 1206 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2822 1870 Size: 73 879 4545 Fragments arranged by size: 4545 879 73 BamHI G'GATC_C Cuts at: 2424 2562 2827 3194 2424 Size: 138 265 367 4727 Fragments arranged by size: 4727 367 265 138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 4273 618 Size: 325 217 627 1031 1455 1842 Fragments arranged by size: 1842 1455 1031 627 325 217 BanII G_rGCy'C Cuts at: 1391 2816 1391 Size: 1425 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2441 2524 928 Size: 971 542 83 3901 Fragments arranged by size: 3901 971 542 83 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2276 3389 4599 2276 Size: 1113 1210 3174 Fragments arranged by size: 3174 1210 1113 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2107 4108 Size: 107 122 71 631 605 337 98 2001 Cuts at: 4108 136 Size: 1525 Fragments arranged by size: 2001 1525 631 605 337 122 107 98 71 BglII A'GATC_T Cuts at: 2511 5492 2511 Size: 2981 2516 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 3558 4714 1790 Size: 1768 1156 2573 Fragments arranged by size: 2573 1768 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 2282 2726 Size: 416 53 83 399 24 668 197 444 Cuts at: 2726 3228 4186 442 Size: 502 958 1753 Fragments arranged by size: 1753 958 668 502 444 416 399 197 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4157 1239 Size: 2918 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3622 4487 4785 2744 Size: 878 865 298 3456 Fragments arranged by size: 3456 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2280 2358 2456 3304 Size: 53 83 186 936 747 78 98 848 Cuts at: 3304 3686 4321 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 848 747 635 382 186 98 83 78 53 BsaWI w'CCGG_w Cuts at: 2598 3930 4328 4602 4749 5160 2598 Size: 1332 398 274 147 411 2935 Fragments arranged by size: 2935 1332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 1178 Size: 30 165 30 778 30 4464 Fragments arranged by size: 4464 778 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5497 BseYI C'CCAG_C Cuts at: 4700 5196 4700 Size: 496 5001 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4924 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2947 3708 4736 1540 Size: 275 186 946 761 1028 2301 Fragments arranged by size: 2301 1028 946 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3558 3643 4714 3558 Size: 85 1071 4341 Fragments arranged by size: 4341 1071 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2816 3558 3643 4714 1391 Size: 399 1026 742 85 1071 2174 Fragments arranged by size: 2174 1071 1026 742 399 85 BspEI T'CCGG_A Cuts at: 2598 5160 2598 Size: 2562 2935 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2852 844 Size: 2008 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 2671 4141 4328 1700 Size: 110 291 570 1470 187 2869 Fragments arranged by size: 2869 1470 570 291 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstXI CCAn_nnnn'nTGG Cuts at: 2276 2276 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2043 2424 2511 2562 2827 3194 3579 3596 Size: 71 381 87 51 265 367 385 17 Cuts at: 3596 5048 5134 5146 5492 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 381 367 346 265 87 86 71 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2303 2303 Size: 5497 BtgI C'CryG_G Cuts at: 513 1065 2004 2150 513 Size: 552 939 146 3860 Fragments arranged by size: 3860 939 552 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 2358 3013 3807 3827 2358 Size: 655 794 20 4028 Fragments arranged by size: 4028 794 655 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462 Size: 54 1425 50 275 18 1114 889 1629 Cuts at: 5462 8 Size: 43 Fragments arranged by size: 1629 1425 1114 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3560 4157 4944 5212 1239 Size: 393 1928 597 787 268 1524 Fragments arranged by size: 1928 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2052 2052 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 2063 2121 4644 1678 Size: 385 58 2523 2531 Fragments arranged by size: 2531 2523 385 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373 Size: 32 135 32 2566 32 42 32 2626 Fragments arranged by size: 2626 2566 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2341 2841 3087 669 Size: 988 281 403 500 246 3079 Fragments arranged by size: 3079 988 500 403 281 246 HindIII A'AGCT_T Cuts at: 748 2762 748 Size: 2014 3483 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2248 4611 4795 1305 Size: 943 2363 184 2007 Fragments arranged by size: 2363 2007 943 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2288 2316 3455 3814 3973 518 Size: 648 1108 14 28 1139 359 159 2042 Fragments arranged by size: 2042 1139 1108 648 359 159 28 14 MspA1I CmG'CkG Cuts at: 1606 2913 3590 4738 4983 1606 Size: 1307 677 1148 245 2120 Fragments arranged by size: 2120 1307 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4006 Fragments arranged by size: 4006 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2524 2855 4400 1132 Size: 97 1295 331 1545 2229 Fragments arranged by size: 2229 1545 1295 331 97 PasI CC'CwG_GG Cuts at: 1348 2718 1348 Size: 1370 4127 PciI A'CATG_T Cuts at: 2520 4396 2520 Size: 1876 3621 PflMI CCAn_nnn'nTGG Cuts at: 1108 2031 1108 Size: 923 4574 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PstI C_TGCA'G Cuts at: 830 2849 830 Size: 2019 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2845 3980 4661 4852 826 Size: 219 1800 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2824 1872 Size: 952 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 3601 4502 4764 820 Size: 197 926 816 842 901 262 1553 Fragments arranged by size: 1553 926 901 842 816 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2004 2793 2936 513 Size: 552 939 789 143 3074 Fragments arranged by size: 3074 939 789 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3524 3541 3694 3879 4324 325 Size: 1255 1944 17 153 185 445 1498 Fragments arranged by size: 1944 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2181 2213 5457 5489 2181 Size: 32 3244 32 2189 Fragments arranged by size: 3244 2189 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2822 1870 Size: 952 4545 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2359 3305 276 Size: 53 83 186 936 825 946 2468 Fragments arranged by size: 2468 946 936 825 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI