Restriction map for
pFN21AB6909
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BpmI BpuAI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MfeI
MluI MroI MseI MslI MunI
NarI NcoI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 65 GGTACC G^GTACC
AfaI 66 GTAC GT^AC
AlwI 261 GATCC GGATC(4/5)
AocI 475 CCTGAGG CC^T[ACGT]AGG
Asp718I 65 GGTACC G^GTACC
BalI 151 TGGCCA TGG^CCA
BanI 65 GGTACC G^G[CT][GA]CC
BcgI 597 CGATGAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 514 CTAG C^TAG
BlpI 228 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 228 GCTCAGC GC^T[ACGT]AGC
BsaAI 160 TACGTG [CT]AC^GT[GA]
BsaBI 534 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 252 GGTCTC GGTCTC(1/5)
BsrBI 376 GAGCGG CCGCTC(-3/-3)
BssHII 224 GCGCGC G^CGCGC
BssSI 370 CACGAG CACGAG(-5/-1)
BstYI 260 AGATCC [GA]^GATC[CT]
Bsu36I 475 CCTGAGG CC^T[ACGT]AGG
CelII 228 GCTCAGC GC^T[ACGT]AGC
CpoI 275 CGGACCG CG^G[AT]CCG
DsaI 491 CCGTGG C^C[GA][CT]GG
EarI 82 CTCTTC CTCTTC(1/4)
Eco81I 475 CCTGAGG CC^T[ACGT]AGG
EcoT14I 338 CCAAGG C^C[AT][AT]GG
HaeII 8 AGCGCC [GA]GCGC^[CT]
HgaI 14 GCGTC GACGC(5/10)
HincII 301 GTCAAC GT[CT]^[GA]AC
HindII 301 GTCAAC GT[CT]^[GA]AC
HinfI 556 GAGTC G^A[ACGT]TC
KpnI 65 GGTACC GGTAC^C
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeI 514 CTAG C^TAG
MaeII 161 ACGT A^CGT
MaeIII 547 GTCAC ^GT[ACGT]AC
MamI 534 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 83 TCTTC GAAGA(8/7)
MflI 260 AGATCC [GA]^GATC[CT]
MluNI 151 TGGCCA TGG^CCA
MscI 151 TGGCCA TGG^CCA
NaeI 233 GCCGGC GCC^GGC
NgoMIV 233 GCCGGC G^CCGGC
NheI 513 GCTAGC G^CTAGC
PleI 556 GAGTC GAGTC(4/5)
PpuMI 47 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 66 GTAC GT^AC
RsrII 275 CGGACCG CG^G[AT]CCG
SfcI 216 CTACAG C^T[GA][CT]AG
StuI 92 AGGCCT AGG^CCT
StyI 338 CCAAGG C^C[AT][AT]GG
Tsp45I 547 GTCAC ^GT[GC]AC
XcmI 338 CCAAGGATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 260 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
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AlwNI 144 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
581 CAGAAGCTG
BsmAI 601 GAGAC GTCTC(1/5)
253 GTCTC
BsrFI 233 GCCGGC [GA]^CCGG[CT]
361 ACCGGC
BsrI 62 ACTGG ACTGG(1/-1)
544 CCAGT
Cfr10I 233 GCCGGC [GA]^CCGG[CT]
361 ACCGGC
EaeI 151 TGGCCA [CT]^GGCC[GA]
203 TGGCCG
NlaIII 391 CATG CATG^
504 CATG
PvuII 42 CAGCTG CAG^CTG
147 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I