(Circular) (Six-base) MAPSORT of: pFN21AB6909.seq Check: 4761 from: 1 to: 5320 pFN21AB6909 5320 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3130 278 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2663 1937 Size: 726 4594 Acc65I G'GTAC_C Cuts at: 2070 2641 2070 Size: 571 4749 AclI AA'CG_TT Cuts at: 1563 3447 3820 1563 Size: 1884 373 3063 Fragments arranged by size: 3063 1884 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3383 4767 5035 1632 Size: 1751 1384 268 1917 Fragments arranged by size: 1917 1751 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5123 AflIII A'CryG_T Cuts at: 1203 4219 5196 1203 Size: 3016 977 1327 Fragments arranged by size: 3016 1327 977 AgeI A'CCGG_T Cuts at: 4151 4151 Size: 5320 AhdI GACnn_n'nnGTC Cuts at: 4049 4049 Size: 5320 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5320 AlwNI CAG_nnn'CTG Cuts at: 2154 2591 3063 4635 2154 Size: 437 472 1572 2839 Fragments arranged by size: 2839 1572 472 437 ApaI G_GGCC'C Cuts at: 2045 2572 2585 2045 Size: 527 13 4780 Fragments arranged by size: 4780 527 13 ApaLI G'TGCA_C Cuts at: 3377 4533 3377 Size: 1156 4164 ApoI r'AATT_y Cuts at: 1493 2629 2855 3007 5116 1493 Size: 1136 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 226 152 AseI AT'TA_AT Cuts at: 160 1032 3874 160 Size: 872 2842 1606 Fragments arranged by size: 2842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 AvaI C'yCGr_G Cuts at: 1870 1943 2645 1870 Size: 73 702 4545 Fragments arranged by size: 4545 702 73 BamHI G'GATC_C Cuts at: 2650 3017 2650 Size: 367 4953 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2641 4096 618 Size: 325 217 627 283 571 1455 1842 Fragments arranged by size: 1842 1455 627 571 325 283 217 BanII G_rGCy'C Cuts at: 1391 2045 2201 2572 2585 2639 1391 Size: 654 156 371 13 54 4072 Fragments arranged by size: 4072 654 371 156 54 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2591 2625 3511 3545 1903 Size: 34 654 34 886 34 3678 Fragments arranged by size: 3678 886 654 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3212 4422 3212 Size: 1210 4110 BclI T'GATC_A Cuts at: 1548 1548 Size: 5320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3931 136 Size: 107 122 71 631 605 2259 1525 Fragments arranged by size: 2259 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5315 5315 Size: 5320 BlpI GC'TnA_GC Cuts at: 2234 2737 2234 Size: 503 4817 Bme1580I G_kGCm'C Cuts at: 1790 2045 2489 2572 2585 3381 4537 1790 Size: 255 444 83 13 796 1156 2573 Fragments arranged by size: 2573 1156 796 444 255 83 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5320 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 3051 4009 Size: 416 53 83 399 24 1126 508 958 Cuts at: 4009 442 Size: 1753 Fragments arranged by size: 1753 1126 958 508 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2522 1056 Size: 1466 3854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3980 1239 Size: 2741 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3445 4310 4608 3445 Size: 865 298 4157 Fragments arranged by size: 4157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2263 3983 882 Size: 617 764 1720 2219 Fragments arranged by size: 2219 1720 764 617 BsaAI yAC'GTr Cuts at: 493 1204 2167 3111 493 Size: 711 963 944 2702 Fragments arranged by size: 2702 963 944 711 BsaBI GATnn'nnATC Cuts at: 2543 5314 2543 Size: 2771 2549 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3127 3509 4144 275 Size: 53 83 186 936 1594 382 635 1451 Fragments arranged by size: 1594 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3753 4151 4425 4572 4983 3753 Size: 398 274 147 411 4090 Fragments arranged by size: 4090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 4354 Fragments arranged by size: 4354 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2393 2458 1186 Size: 952 255 65 4048 Fragments arranged by size: 4048 952 255 65 BseYI C'CCAG_C Cuts at: 2045 2049 2571 4523 5019 2045 Size: 4 522 1952 496 2346 Fragments arranged by size: 2346 1952 522 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4747 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2770 3531 4559 1540 Size: 275 186 769 761 1028 2301 Fragments arranged by size: 2301 1028 769 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3381 3466 4537 3381 Size: 85 1071 4164 Fragments arranged by size: 4164 1071 85 BsmI GAATG_Cn' Cuts at: 2831 2924 2831 Size: 93 5227 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2201 2489 2572 2585 2639 3381 Size: 399 255 156 288 83 13 54 742 Cuts at: 3381 3466 4537 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 288 255 156 85 83 54 13 BspEI T'CCGG_A Cuts at: 4983 4983 Size: 5320 BspHI T'CATG_A Cuts at: 3209 4939 3209 Size: 1730 3590 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2675 844 Size: 1831 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2383 3207 1394 Size: 429 560 824 3507 Fragments arranged by size: 3507 824 560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3815 3989 65 Size: 1183 2567 174 1396 Fragments arranged by size: 2567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2238 2366 3964 4151 1700 Size: 110 428 128 1598 187 2869 Fragments arranged by size: 2869 1598 428 187 128 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5320 BssHII G'CGCG_C Cuts at: 1925 2229 1925 Size: 304 5016 BssSI C'ACGA_G Cuts at: 2375 3380 4392 2375 Size: 1005 1012 3303 Fragments arranged by size: 3303 1012 1005 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5320 BstBI TT'CG_AA Cuts at: 4124 4124 Size: 5320 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5320 BstYI r'GATC_y Cuts at: 1972 2265 2650 3017 3402 3419 4871 4957 4969 Size: 293 385 367 385 17 1452 86 12 Cuts at: 4969 5315 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 385 367 346 293 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2481 2481 Size: 5320 BtgI C'CryG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3337 Fragments arranged by size: 3337 1431 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4581 Fragments arranged by size: 4581 683 56 BtsI GCAGTG_nn' Cuts at: 2141 2211 2274 2836 3630 3650 2141 Size: 70 63 562 794 20 3811 Fragments arranged by size: 3811 794 562 70 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5285 DraI TTT'AAA Cuts at: 2624 2624 Size: 5320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4327 809 Size: 507 3011 1802 Fragments arranged by size: 3011 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2156 2208 2767 Size: 54 1425 50 275 18 326 52 559 Cuts at: 2767 3656 5285 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 559 326 275 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2767 1537 Size: 275 955 4090 Fragments arranged by size: 4090 955 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 3250 1353 Size: 740 1157 3423 Fragments arranged by size: 3423 1157 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3907 4281 4427 387 Size: 1064 138 2318 374 146 1280 Fragments arranged by size: 2318 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3383 3980 4767 5035 1239 Size: 393 1751 597 787 268 1524 Fragments arranged by size: 1751 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2041 2042 2053 2503 2581 2041 Size: 1 11 450 78 4780 Fragments arranged by size: 4780 450 78 11 1 EcoRI G'AATT_C Cuts at: 2629 3007 2629 Size: 378 4942 FspI TGC'GCA Cuts at: 3826 3826 Size: 5320 HaeII r_GCGC'y Cuts at: 1678 2017 4467 1678 Size: 339 2450 2531 Fragments arranged by size: 2531 2450 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 3961 3993 4035 Size: 32 135 32 542 32 1815 32 42 Cuts at: 4035 4067 1373 Size: 32 2626 Fragments arranged by size: 2626 1815 542 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 2664 2910 669 Size: 988 281 370 356 246 3079 Fragments arranged by size: 3079 988 370 356 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5320 HpaI GTT'AAC Cuts at: 2910 2910 Size: 5320 KpnI G_GTAC'C Cuts at: 2074 2645 2074 Size: 571 4749 MfeI C'AATT_G Cuts at: 2919 2919 Size: 5320 MluI A'CGCG_T Cuts at: 5196 5196 Size: 5320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 4434 4618 1305 Size: 1251 1878 184 2007 Fragments arranged by size: 2007 1878 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2158 10 Size: 54 1425 343 326 3172 Fragments arranged by size: 3172 1425 343 326 54 MslI CAynn'nnrTG Cuts at: 518 1166 3278 3637 3796 518 Size: 648 2112 359 159 2042 Fragments arranged by size: 2112 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2154 2253 2286 2736 3413 4561 4806 Size: 443 105 99 33 450 677 1148 245 Cuts at: 4806 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 450 443 245 105 99 33 NaeI GCC'GGC Cuts at: 2240 2240 Size: 5320 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4768 NdeI CA'TA_TG Cuts at: 387 387 Size: 5320 NgoMIV G'CCGG_C Cuts at: 2238 2238 Size: 5320 NheI G'CTAG_C Cuts at: 1052 2518 1052 Size: 1466 3854 NotI GC'GGCC_GC Cuts at: 2767 2767 Size: 5320 NspI r_CATG'y Cuts at: 1132 1229 2678 4223 1132 Size: 97 1449 1545 2229 Fragments arranged by size: 2229 1545 1449 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5320 PciI A'CATG_T Cuts at: 4219 4219 Size: 5320 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5320 PmeI GTTT'AAAC Cuts at: 2624 2624 Size: 5320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4902 4934 3389 Size: 32 1481 32 3775 Fragments arranged by size: 3775 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2053 Size: 5320 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5320 PsiI TTA'TAA Cuts at: 2890 2890 Size: 5320 PspOMI G'GGCC_C Cuts at: 2041 2568 2581 2041 Size: 527 13 4780 Fragments arranged by size: 4780 527 13 PstI C_TGCA'G Cuts at: 830 2672 830 Size: 1842 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5320 PvuII CAG'CTG Cuts at: 2049 2154 2049 Size: 105 5215 RsrII CG'GwC_CG Cuts at: 2281 2281 Size: 5320 SalI G'TCGA_C Cuts at: 1936 2662 1936 Size: 726 4594 SanDI GG'GwC_CC Cuts at: 2053 2053 Size: 5320 SbfI CC_TGCA'GG Cuts at: 2672 2672 Size: 5320 ScaI AGT'ACT Cuts at: 3568 5280 3568 Size: 1712 3608 SfcI C'TryA_G Cuts at: 826 1045 2221 2668 3803 4484 4675 826 Size: 219 1176 447 1135 681 191 1471 Fragments arranged by size: 1471 1176 1135 681 447 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5320 SmaI CCC'GGG Cuts at: 1872 2647 1872 Size: 775 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3424 4325 4587 820 Size: 197 926 1481 901 262 1553 Fragments arranged by size: 1553 1481 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3111 493 Size: 2618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5320 SphI G_CATG'C Cuts at: 1132 2678 1132 Size: 1546 3774 SspI AAT'ATT Cuts at: 5 52 3244 5 Size: 47 3192 2081 Fragments arranged by size: 3192 2081 47 StuI AGG'CCT Cuts at: 1517 2099 1517 Size: 582 4738 StyI C'CwwG_G Cuts at: 513 1065 2343 2616 2759 513 Size: 552 1278 273 143 3074 Fragments arranged by size: 3074 1278 552 273 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2471 3347 3364 3517 3702 4147 Size: 1255 444 447 876 17 153 185 445 Cuts at: 4147 325 Size: 1498 Fragments arranged by size: 1498 1255 876 447 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3566 5278 96 Size: 274 80 33 51 1443 1589 1712 138 Fragments arranged by size: 1712 1589 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5280 5312 5280 Size: 32 5288 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5320 XbaI T'CTAG_A Cuts at: 2656 2656 Size: 5320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 5320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5320 XmaI C'CCGG_G Cuts at: 1870 2645 1870 Size: 775 4545 XmnI GAAnn'nnTTC Cuts at: 3449 3449 Size: 5320 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3128 276 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SapI SexAI SfiI SfoI SrfI SwaI