Restriction map for pFN21AB6939
 (Circular) (Six-base) MAPSORT of: pFN21AB6939.seq  Check: 4857  from: 1  to: 5329



pFN21AB6939  5329 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2426    3139     278 
   Size:         53      83     186     936     890     713    2468

  Fragments arranged by size: 

                2468     936     890     713     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2672    1937 
   Size:        735    4594
 
Acc65I G'GTAC_C

Cuts at:   2650    2650 
   Size:       5329
 
AclI AA'CG_TT

Cuts at:   1563    3456    3829    1563 
   Size:       1893     373    3063

  Fragments arranged by size: 

                3063    1893     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3392    4776    5044    1632 
   Size:       1760    1384     268    1917

  Fragments arranged by size: 

                1917    1760    1384     268
 
AfeI AGC'GCT

Cuts at:   2105    2468    2105 
   Size:        363    4966
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5132
 
AflIII A'CryG_T

Cuts at:   1203    2376    4228    5205    1203 
   Size:       1173    1852     977    1327

  Fragments arranged by size: 

                1852    1327    1173     977
 
AgeI A'CCGG_T

Cuts at:   2573    4160    2573 
   Size:       1587    3742
 
AhdI GACnn_n'nnGTC

Cuts at:   4058    4058 
   Size:       5329
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5329
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5329
 
AlwNI CAG_nnn'CTG

Cuts at:   3072    4644    3072 
   Size:       1572    3757
 
ApaI G_GGCC'C

Cuts at:   2334    2334 
   Size:       5329
 
ApaLI G'TGCA_C

Cuts at:   3386    4542    3386 
   Size:       1156    4173
 
ApoI r'AATT_y

Cuts at:   1493    2638    2864    3016    5125    1493 
   Size:       1145     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1145     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3883     160 
   Size:        872    2851    1606

  Fragments arranged by size: 

                2851    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5329
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2126    2292    2654    1870 
   Size:         73     183     166     362    4545

  Fragments arranged by size: 

                4545     362     183     166      73
 
BamHI G'GATC_C

Cuts at:   2659    3026    2659 
   Size:        367    4962
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2442    2650    4105     618 
   Size:        325     217     627     655     208    1455    1842

  Fragments arranged by size: 

                1842    1455     655     627     325     217     208
 
BanII G_rGCy'C

Cuts at:   1391    2063    2225    2334    2373    2648    1391 
   Size:        672     162     109      39     275    4072

  Fragments arranged by size: 

                4072     672     275     162     109      39
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2483     928 
   Size:        971     584    3774

  Fragments arranged by size: 

                3774     971     584
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3520    3554    1903 
   Size:         34    1583      34    3678

  Fragments arranged by size: 

                3678    1583      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3221    4431    3221 
   Size:       1210    4119
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5329
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3940     136 
   Size:        107     122      71     631     605    2268    1525

  Fragments arranged by size: 

                2268    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5324    5324 
   Size:       5329
 
BlpI GC'TnA_GC

Cuts at:   2746    2746 
   Size:       5329
 
Bme1580I G_kGCm'C

Cuts at:   1790    2334    2384    2499    3390    4546    1790 
   Size:        544      50     115     891    1156    2573

  Fragments arranged by size: 

                2573    1156     891     544     115      50
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5329
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3060    4018     442 
   Size:        416      53      83     399      24    1643     958    1753

  Fragments arranged by size: 

                1753    1643     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5329
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2329    3989    1239 
   Size:       1090    1660    2579

  Fragments arranged by size: 

                2579    1660    1090
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2078    3454    4319    4617    2078 
   Size:       1376     865     298    2790

  Fragments arranged by size: 

                2790    1376     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3992     882 
   Size:        617    2493    2219

  Fragments arranged by size: 

                2493    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2379    3120     493 
   Size:        711    1175     741    2702

  Fragments arranged by size: 

                2702    1175     741     711
 
BsaBI GATnn'nnATC

Cuts at:   5323    5323 
   Size:       5329
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2123    2423    3136    3518 
   Size:         53      83     186     936     590     300     713     382

Cuts at:   3518    4153     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     713     635     590     382     300     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   2573    3762    4160    4434    4581    4992    2573 
   Size:       1189     398     274     147     411    2910

  Fragments arranged by size: 

                2910    1189     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5104

  Fragments arranged by size: 

                5104     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2315    1186 
   Size:       1129    4200
 
BseYI C'CCAG_C

Cuts at:   2333    4532    5028    2333 
   Size:       2199     496    2634

  Fragments arranged by size: 

                2634    2199     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4756
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2779    3540    4568    1540 
   Size:        275     186     778     761    1028    2301

  Fragments arranged by size: 

                2301    1028     778     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2063    2508    3390    3475    4546    2063 
   Size:        445     882      85    1071    2846

  Fragments arranged by size: 

                2846    1071     882     445      85
 
BsmI GAATG_Cn'

Cuts at:   2840    2933    2840 
   Size:         93    5236
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2430    2430 
   Size:       5329
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2063    2225    2334    2373    2384    2499    2508 
   Size:        399     273     162     109      39      11     115       9

Cuts at:   2508    2648    3390    3475    4546    1391 
   Size:        140     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     273     162     140     115
                 109      85      39      11       9
 
BspEI T'CCGG_A

Cuts at:   4992    4992 
   Size:       5329
 
BspHI T'CATG_A

Cuts at:   3218    4948    3218 
   Size:       1730    3599
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2251    2684     844 
   Size:       1407     433    3489

  Fragments arranged by size: 

                3489    1407     433
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2305    2316    3216    1394 
   Size:        429     482      11     900    3507

  Fragments arranged by size: 

                3507     900     482     429      11
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3824    3998      65 
   Size:       1183    2576     174    1396

  Fragments arranged by size: 

                2576    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2267    2445    2573    3973    4160    1700 
   Size:        110     457     178     128    1400     187    2869

  Fragments arranged by size: 

                2869    1400     457     187     178     128     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5329
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5329
 
BssSI C'ACGA_G

Cuts at:   3389    4401    3389 
   Size:       1012    4317
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5329
 
BstBI TT'CG_AA

Cuts at:   4133    4133 
   Size:       5329
 
BstEII G'GTnAC_C

Cuts at:   1184    2263    1184 
   Size:       1079    4250
 
BstYI r'GATC_y

Cuts at:   1972    2659    3026    3411    3428    4880    4966    4978    5324 
   Size:        687     367     385      17    1452      86      12     346

Cuts at:   5324    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     687     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2286     513 
   Size:        552     939     282    3556

  Fragments arranged by size: 

                3556     939     552     282
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4590

  Fragments arranged by size: 

                4590     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2845    3639    3659    2845 
   Size:        794      20    4515

  Fragments arranged by size: 

                4515     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5329
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5294
 
DraI TTT'AAA

Cuts at:   2633    2633 
   Size:       5329
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4336     809 
   Size:        507    3020    1802

  Fragments arranged by size: 

                3020    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2512    2776    3665 
   Size:         54    1425      50     275      18     682     264     889

Cuts at:   3665    5294       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     682     275     264      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2776    1537 
   Size:        275     964    4090

  Fragments arranged by size: 

                4090     964     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2555    3259    1353 
   Size:       1202     704    3423

  Fragments arranged by size: 

                3423    1202     704
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2264    3916    4290    4436     387 
   Size:       1064     138     675    1652     374     146    1280

  Fragments arranged by size: 

                1652    1280    1064     675     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2061    2061 
   Size:       5329
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2329    3392    3989    4776    5044    1239 
   Size:        393     697    1063     597     787     268    1524

  Fragments arranged by size: 

                1524    1063     787     697     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2069    2249    2069 
   Size:        180    5149
 
EcoO109I rG'GnC_Cy

Cuts at:   2281    2330    2605    2281 
   Size:         49     275    5005

  Fragments arranged by size: 

                5005     275      49
 
EcoRI G'AATT_C

Cuts at:   2638    3016    2638 
   Size:        378    4951
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2422    2422 
   Size:       5329
 
FspI TGC'GCA

Cuts at:   3835    3835 
   Size:       5329
 
HaeII r_GCGC'y

Cuts at:   1678    2107    2470    4476    1678 
   Size:        429     363    2006    2531

  Fragments arranged by size: 

                2531    2006     429     363
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3970    4002    4044    4076    1373 
   Size:         32     135      32    2398      32      42      32    2626

  Fragments arranged by size: 

                2626    2398     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2673    2919     669 
   Size:        988     281     735     246    3079

  Fragments arranged by size: 

                3079     988     735     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5329
 
HpaI GTT'AAC

Cuts at:   2919    2919 
   Size:       5329
 
KpnI G_GTAC'C

Cuts at:   2654    2654 
   Size:       5329
 
MfeI C'AATT_G

Cuts at:   2928    2928 
   Size:       5329
 
MluI A'CGCG_T

Cuts at:   5205    5205 
   Size:       5329
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4443    4627    1305 
   Size:       3138     184    2007

  Fragments arranged by size: 

                3138    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3507

  Fragments arranged by size: 

                3507    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3287    3646    3805     518 
   Size:        648    2121     359     159    2042

  Fragments arranged by size: 

                2121    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2288    2745    3422    4570    4815    1606 
   Size:        682     457     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     682     677     457     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3838

  Fragments arranged by size: 

                3838     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5329
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5329
 
NotI GC'GGCC_GC

Cuts at:   2776    2776 
   Size:       5329
 
NspI r_CATG'y

Cuts at:   1132    1229    2687    4232    1132 
   Size:         97    1458    1545    2229

  Fragments arranged by size: 

                2229    1545    1458      97
 
PasI CC'CwG_GG

Cuts at:   1348    2065    1348 
   Size:        717    4612
 
PciI A'CATG_T

Cuts at:   4228    4228 
   Size:       5329
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5329
 
PmeI GTTT'AAAC

Cuts at:   2633    2633 
   Size:       5329
 
PmlI CAC'GTG

Cuts at:   2379    2379 
   Size:       5329
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3398    3430    4911    4943    3398 
   Size:         32    1481      32    3784

  Fragments arranged by size: 

                3784    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2281    2605    2281 
   Size:        324    5005
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5329
 
PsiI TTA'TAA

Cuts at:   2899    2899 
   Size:       5329
 
PspOMI G'GGCC_C

Cuts at:   2330    2330 
   Size:       5329
 
PstI C_TGCA'G

Cuts at:    830    2111    2393    2681     830 
   Size:       1281     282     288    3478

  Fragments arranged by size: 

                3478    1281     288     282
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5329
 
SacI G_AGCT'C

Cuts at:   2063    2063 
   Size:       5329
 
SacII CC_GC'GG

Cuts at:   2289    2289 
   Size:       5329
 
SalI G'TCGA_C

Cuts at:   1936    2671    1936 
   Size:        735    4594
 
SanDI GG'GwC_CC

Cuts at:   2605    2605 
   Size:       5329
 
SbfI CC_TGCA'GG

Cuts at:   2681    2681 
   Size:       5329
 
ScaI AGT'ACT

Cuts at:   3577    5289    3577 
   Size:       1712    3617
 
SfcI C'TryA_G

Cuts at:    826    1045    2107    2389    2677    3812    4493    4684     826 
   Size:        219    1062     282     288    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1062     681     288     282     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5329
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2573    1700 
   Size:        873    4456
 
SmaI CCC'GGG

Cuts at:   1872    2128    2656    1872 
   Size:        256     528    4545

  Fragments arranged by size: 

                4545     528     256
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2093    3433    4334    4596     820 
   Size:        197     926     150    1340     901     262    1553

  Fragments arranged by size: 

                1553    1340     926     901     262     197     150
 
SnaBI TAC'GTA

Cuts at:    493    3120     493 
   Size:       2627    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5329
 
SphI G_CATG'C

Cuts at:   1132    2687    1132 
   Size:       1555    3774
 
SspI AAT'ATT

Cuts at:      5      52    3253       5 
   Size:         47    3201    2081

  Fragments arranged by size: 

                3201    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5329
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2559    2768     513 
   Size:        552     939     555     209    3074

  Fragments arranged by size: 

                3074     939     555     552     209
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3356    3373    3526    3711    4156     325 
   Size:       1255    1776      17     153     185     445    1498

  Fragments arranged by size: 

                1776    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2040    2403    3575 
   Size:        274      80      33      51    1443      63     363    1172

Cuts at:   3575    5287      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1172     363     274     138      80      63
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5289    5321    5289 
   Size:         32    5297
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5329
 
XbaI T'CTAG_A

Cuts at:   2665    2665 
   Size:       5329
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5329
 
XmaI C'CCGG_G

Cuts at:   1870    2126    2654    1870 
   Size:        256     528    4545

  Fragments arranged by size: 

                4545     528     256
 
XmnI GAAnn'nnTTC

Cuts at:   2156    3458    2156 
   Size:       1302    4027
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2424    3137     276 
   Size:         53      83     186     936     890     713    2468

  Fragments arranged by size: 

                2468     936     890     713     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     PvuI     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI    PspXI     PsrI    PvuII    RsrII     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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