(Circular) (Six-base) MAPSORT of: pFN21AE3669.seq Check: 2452 from: 1 to: 6094 pFN21AE3669 6094 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3437 1937 Size: 1500 4594 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 3149 3206 4157 5541 5809 1632 Size: 581 936 57 951 1384 268 1917 Fragments arranged by size: 1917 1384 951 936 581 268 57 AfeI AGC'GCT Cuts at: 2501 2501 Size: 6094 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 2760 2946 2976 4993 5970 1203 Size: 1557 186 30 2017 977 1327 Fragments arranged by size: 2017 1557 1327 977 186 30 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 2992 4823 2992 Size: 1831 4263 AleI CACnn'nnGTG Cuts at: 1166 2867 1166 Size: 1701 4393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3030 1184 Size: 1846 4248 AlwNI CAG_nnn'CTG Cuts at: 3837 5409 3837 Size: 1572 4522 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 3403 3629 3781 5890 1493 Size: 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 3052 3419 1870 Size: 73 1109 367 4545 Fragments arranged by size: 4545 1109 367 73 BamHI G'GATC_C Cuts at: 2159 3424 3791 2159 Size: 1265 367 4462 Fragments arranged by size: 4462 1265 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2788 3217 3344 3415 4870 Size: 325 217 627 1001 429 127 71 1455 Cuts at: 4870 618 Size: 1842 Fragments arranged by size: 1842 1455 1001 627 429 325 217 127 71 BanII G_rGCy'C Cuts at: 1391 2616 3413 1391 Size: 1225 797 4072 Fragments arranged by size: 4072 1225 797 BbeI G_GCGC'C Cuts at: 3348 3348 Size: 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2024 928 Size: 971 125 4998 Fragments arranged by size: 4998 971 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2203 2237 4285 4319 1903 Size: 34 266 34 2048 34 3678 Fragments arranged by size: 3678 2048 266 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3986 5196 3986 Size: 1210 4884 BclI T'GATC_A Cuts at: 1548 2310 1548 Size: 762 5332 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 4705 136 Size: 107 122 71 631 605 748 2285 1525 Fragments arranged by size: 2285 1525 748 631 605 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 2571 2580 2793 4155 5311 1790 Size: 781 9 213 1362 1156 2573 Fragments arranged by size: 2573 1362 1156 781 213 9 BmgBI CAC'GTC Cuts at: 1318 2761 1318 Size: 1443 4651 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3247 3825 4783 Size: 416 53 83 399 24 1830 578 958 Cuts at: 4783 442 Size: 1753 Fragments arranged by size: 1830 1753 958 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2925 3265 2925 Size: 340 5754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2630 2711 3185 3213 4754 1239 Size: 1391 81 474 28 1541 2579 Fragments arranged by size: 2579 1541 1391 474 81 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4219 5084 5382 4219 Size: 865 298 4931 Fragments arranged by size: 4931 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2240 2801 4757 882 Size: 617 741 561 1956 2219 Fragments arranged by size: 2219 1956 741 617 561 BsaAI yAC'GTr Cuts at: 493 1204 2050 3885 493 Size: 711 846 1835 2702 Fragments arranged by size: 2702 1835 846 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3312 3345 3901 4283 Size: 53 83 186 936 1779 33 556 382 Cuts at: 4283 4918 275 Size: 635 1451 Fragments arranged by size: 1779 1451 936 635 556 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 2057 4527 4925 5199 5346 5757 2057 Size: 2470 398 274 147 411 2394 Fragments arranged by size: 2470 2394 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 1178 Size: 30 165 30 636 30 5203 Fragments arranged by size: 5203 636 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6094 BseYI C'CCAG_C Cuts at: 2431 5297 5793 2431 Size: 2866 496 2732 Fragments arranged by size: 2866 2732 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5521 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2825 3271 3544 4305 5333 1540 Size: 275 186 824 446 273 761 1028 2301 Fragments arranged by size: 2301 1028 824 761 446 275 273 186 BsiHKAI G_wGCw'C Cuts at: 4155 4240 5311 4155 Size: 85 1071 4938 Fragments arranged by size: 4938 1071 85 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 BsmBI CGTCTCn'nnnn_ Cuts at: 3189 3189 Size: 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2571 2580 2616 2793 3413 4155 4240 Size: 399 781 9 36 177 620 742 85 Cuts at: 4240 5311 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 781 742 620 399 177 85 36 9 BspEI T'CCGG_A Cuts at: 2057 5757 2057 Size: 3700 2394 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3449 844 Size: 2605 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3339 3981 1394 Size: 429 1516 642 3507 Fragments arranged by size: 3507 1516 642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2482 2810 3068 3144 3347 4738 4925 Size: 110 672 328 258 76 203 1391 187 Cuts at: 4925 1700 Size: 2869 Fragments arranged by size: 2869 1391 672 328 258 203 187 110 76 BsrGI T'GTAC_A Cuts at: 96 2763 96 Size: 2667 3427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 2891 4154 5166 2891 Size: 1263 1012 3819 Fragments arranged by size: 3819 1263 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2416 794 Size: 1622 4472 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 2836 3355 1184 Size: 1652 519 3923 Fragments arranged by size: 3923 1652 519 BstXI CCAn_nnnn'nTGG Cuts at: 3238 3238 Size: 6094 BstYI r'GATC_y Cuts at: 1972 2159 3424 3791 4176 4193 5645 5731 5743 Size: 187 1265 367 385 17 1452 86 12 Cuts at: 5743 6089 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1265 385 367 346 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 2630 2727 2793 513 Size: 552 939 69 557 97 66 3814 Fragments arranged by size: 3814 939 557 552 97 69 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3091 487 Size: 683 56 1865 3490 Fragments arranged by size: 3490 1865 683 56 BtsI GCAGTG_nn' Cuts at: 2346 3610 4404 4424 2346 Size: 1264 794 20 4016 Fragments arranged by size: 4016 1264 794 20 ClaI AT'CG_AT Cuts at: 1554 2274 1554 Size: 720 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2962 2997 529 Size: 35 2398 35 3626 Fragments arranged by size: 3626 2398 35 35 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2513 5101 809 Size: 507 1197 2588 1802 Fragments arranged by size: 2588 1802 1197 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2284 2559 Size: 54 1425 50 275 18 214 240 275 Cuts at: 2559 2822 3201 3268 3303 3541 4430 6059 8 Size: 263 379 67 35 238 889 1629 43 Fragments arranged by size: 1629 1425 889 379 275 275 263 240 238 214 67 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2822 3268 3541 1537 Size: 275 1010 446 273 4090 Fragments arranged by size: 4090 1010 446 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 3329 4024 1353 Size: 1976 695 3423 Fragments arranged by size: 3423 1976 695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 2345 4681 5055 5201 387 Size: 1064 138 624 132 2336 374 146 1280 Fragments arranged by size: 2336 1280 1064 624 374 146 138 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2630 2711 3149 3185 3206 3213 Size: 393 581 417 81 438 36 21 7 Cuts at: 3213 4157 4754 5541 5809 1239 Size: 944 597 787 268 1524 Fragments arranged by size: 1524 944 787 597 581 438 417 393 268 81 36 21 7 EcoNI CCTnn'n_nnAGG Cuts at: 2690 2690 Size: 6094 EcoO109I rG'GnC_Cy Cuts at: 2695 2695 Size: 6094 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2179 Size: 6094 FspI TGC'GCA Cuts at: 2338 4600 2338 Size: 2262 3832 HaeII r_GCGC'y Cuts at: 1678 2503 2532 3069 3151 3348 5241 1678 Size: 825 29 537 82 197 1893 2531 Fragments arranged by size: 2531 1893 825 537 197 82 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2858 2890 2978 3010 3134 Size: 32 135 32 1286 32 88 32 124 Cuts at: 3134 3166 4735 4767 4809 4841 1373 Size: 32 1569 32 42 32 2626 Fragments arranged by size: 2626 1569 1286 135 124 88 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3438 3684 669 Size: 988 281 1500 246 3079 Fragments arranged by size: 3079 1500 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 3344 3344 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5208 5392 1305 Size: 3903 184 2007 Fragments arranged by size: 3903 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4272 Fragments arranged by size: 4272 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2834 2867 3236 4052 4411 4570 518 Size: 648 1668 33 369 816 359 159 2042 Fragments arranged by size: 2042 1668 816 648 369 359 159 33 MspA1I CmG'CkG Cuts at: 1606 2752 3510 4187 5335 5580 1606 Size: 1146 758 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 758 677 245 NaeI GCC'GGC Cuts at: 2484 3070 3349 2484 Size: 586 279 5229 Fragments arranged by size: 5229 586 279 NarI GG'CG_CC Cuts at: 3345 3345 Size: 6094 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4603 Fragments arranged by size: 4603 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6094 NgoMIV G'CCGG_C Cuts at: 2482 3068 3347 2482 Size: 586 279 5229 Fragments arranged by size: 5229 586 279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 2414 2608 2629 3452 4997 1132 Size: 97 1185 194 21 823 1545 2229 Fragments arranged by size: 2229 1545 1185 823 194 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6094 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PmlI CAC'GTG Cuts at: 2050 2050 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PstI C_TGCA'G Cuts at: 830 2757 3446 830 Size: 1927 689 3478 Fragments arranged by size: 3478 1927 689 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SapI GCTCTTCn'nnn_ Cuts at: 3329 3329 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 2258 4342 6054 2258 Size: 2084 1712 2298 Fragments arranged by size: 2298 2084 1712 SfcI C'TryA_G Cuts at: 826 1045 2488 2753 3442 4577 5258 5449 826 Size: 219 1443 265 689 1135 681 191 1471 Fragments arranged by size: 1471 1443 1135 689 681 265 219 191 SfoI GGC'GCC Cuts at: 3346 3346 Size: 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 3054 3421 1872 Size: 1182 367 4545 Fragments arranged by size: 4545 1182 367 SmlI C'TyrA_G Cuts at: 820 1017 1943 4198 5099 5361 820 Size: 197 926 2255 901 262 1553 Fragments arranged by size: 2255 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 2414 3452 1132 Size: 1282 1038 3774 Fragments arranged by size: 3774 1282 1038 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6094 StyI C'CwwG_G Cuts at: 513 1065 2004 3533 513 Size: 552 939 1529 3074 Fragments arranged by size: 3074 1529 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2167 2423 4121 4138 4291 4476 4921 Size: 1255 587 256 1698 17 153 185 445 Cuts at: 4921 325 Size: 1498 Fragments arranged by size: 1698 1498 1255 587 445 256 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 2763 4340 Size: 274 80 33 51 1443 279 507 1577 Cuts at: 4340 6052 96 Size: 1712 138 Fragments arranged by size: 1712 1577 1443 507 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 6054 6086 2039 Size: 32 3983 32 2047 Fragments arranged by size: 3983 2047 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2924 1316 Size: 1608 4486 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3211 3211 Size: 6094 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 3052 3419 1870 Size: 1182 367 4545 Fragments arranged by size: 4545 1182 367 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI