Restriction map for
pFN21AE1446
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AvaI AviII
AvrII BbeI BbrPI BbvCI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 958 GAATTT [GA]^AATT[CT]
AhdI 681 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 958 GAATTT [GA]^AATT[CT]
Asp700I 669 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 681 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 612 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 384 GGATCC G^GATCC
BanI 136 GGCACC G^G[CT][GA]CC
BbsI 669 GAAGAC GAAGAC(2/6)
BcgI 8 CGATCCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 929 GTATCC GTATCC(6/5)
BcnI 563 CCCGG CC^[GC]GG
BlpI 209 GCTCAGC GC^T[ACGT]AGC
BpmI 530 CTGGAG CTGGAG(16/14)
Bpu1102I 209 GCTCAGC GC^T[ACGT]AGC
BpuAI 669 GAAGAC GAAGAC(2/6)
BsaI 65 GAGACC GGTCTC(1/5)
BseRI 70 CTCCTC GAGGAG(10/8)
BsmBI 229 CGTCTC CGTCTC(1/5)
BsrBI 28 GAGCGG CCGCTC(-3/-3)
BsrDI 431 GCAATG GCAATG(2/0)
BstBI 955 TTCGAA TT^CGAA
CelII 209 GCTCAGC GC^T[ACGT]AGC
Eam1105I 681 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 381 GCGGG CCCGC(4/6)
HincII 694 GTCAAC GT[CT]^[GA]AC
HindII 694 GTCAAC GT[CT]^[GA]AC
HindIII 637 AAGCTT A^AGCTT
NciI 563 CCCGG CC^[GC]GG
NcoI 755 CCATGG C^CATGG
NspI 195 GCATGT [GA]CATG^[CT]
NspV 955 TTCGAA TT^CGAA
PflFI 612 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 16 CTGCAG CTGCA^G
SacI 505 GAGCTC GAGCT^C
ScaI 950 AGTACT AGT^ACT
SfaNI 479 GCATC GCATC(5/9)
SfuI 955 TTCGAA TT^CGAA
SmlI 268 CTCAAG C^T[CT][GA]AG
Tth111I 612 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 669 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccII 58 CGCG CG^CG
615 CGCG
AccIII 287 TCCGGA T^CCGGA
677 TCCGGA
AfaI 111 GTAC GT^AC
951 GTAC
AspHI 505 GAGCTC G[AT]GC[AT]^C
556 GAGCAC
BalI 83 TGGCCA TGG^CCA
489 TGGCCA
BanII 409 GAGCCC G[GA]GC[CT]^C
505 GAGCTC
BseAI 287 TCCGGA T^CCGGA
677 TCCGGA
BsiHKAI 505 GAGCTC G[AT]GC[AT]^C
556 GAGCAC
BsiYI 12 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
773 CCTCCACCAGG
BslI 12 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
773 CCTCCACCAGG
BsmAI 65 GAGAC GTCTC(1/5)
230 GTCTC
BspEI 287 TCCGGA T^CCGGA
677 TCCGGA
BspMI 292 ACCTGC ACCTGC(4/8)
377 ACCTGC
BstUI 58 CGCG CG^CG
615 CGCG
BstXI 492 CCATGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
879 CCATCCTGATGG
BstYI 384 GGATCC [GA]^GATC[CT]
422 AGATCC
Cac8I 258 GCTGGC GC[ACGT]^[ACGT]GC
487 GCTGGC
DdeI 210 CTCAG C^T[ACGT]AG
699 CTCAG
DraII 89 AGGACCC [GA]G^G[ACGT]CC[CT]
462 GGGACCC
DrdI 91 GACCCTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
724 GACCATGTGGTC
DsaI 332 CCACGG C^C[GA][CT]GG
755 CCATGG
EaeI 83 TGGCCA [CT]^GGCC[GA]
489 TGGCCA
EarI 826 GAAGAG CTCTTC(1/4)
836 GAAGAG
EcoNI 12 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
773 CCTCCACCAGG
EcoO109I 89 AGGACCC [GA]G^G[ACGT]CC[CT]
462 GGGACCC
HgaI 175 GACGC GACGC(5/10)
616 GCGTC
Ksp632I 826 GAAGAG CTCTTC(1/4)
836 GAAGAG
MaeII 687 ACGT A^CGT
923 ACGT
MflI 384 GGATCC [GA]^GATC[CT]
422 AGATCC
MluNI 83 TGGCCA TGG^CCA
489 TGGCCA
MroI 287 TCCGGA T^CCGGA
677 TCCGGA
MscI 83 TGGCCA TGG^CCA
489 TGGCCA
MseI 47 TTAA T^TAA
551 TTAA
MvnI 58 CGCG CG^CG
615 CGCG
PpuMI 89 AGGACCC [GA]G^G[AT]CC[CT]
462 GGGACCC
RsaI 111 GTAC GT^AC
951 GTAC
SfcI 16 CTGCAG C^T[GA][CT]AG
897 CTACAG
TaqI 941 TCGA T^CGA
956 TCGA
Tru9I 47 TTAA T^TAA
551 TTAA
Tsp45I 610 GTGAC ^GT[GC]AC
848 GTCAC
Tsp509I 277 AATT ^AATT
959 AATT
TthHB8I 941 TCGA T^CGA
956 TCGA
XhoII 384 GGATCC [GA]^GATC[CT]
422 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BsaJI BsaWI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI StyI TseI
TspRI