################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BbeI BbrPI BbvCI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 958 GAATTT [GA]^AATT[CT] AhdI 681 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 958 GAATTT [GA]^AATT[CT] Asp700I 669 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 681 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 612 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 384 GGATCC G^GATCC BanI 136 GGCACC G^G[CT][GA]CC BbsI 669 GAAGAC GAAGAC(2/6) BcgI 8 CGATCCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 929 GTATCC GTATCC(6/5) BcnI 563 CCCGG CC^[GC]GG BlpI 209 GCTCAGC GC^T[ACGT]AGC BpmI 530 CTGGAG CTGGAG(16/14) Bpu1102I 209 GCTCAGC GC^T[ACGT]AGC BpuAI 669 GAAGAC GAAGAC(2/6) BsaI 65 GAGACC GGTCTC(1/5) BseRI 70 CTCCTC GAGGAG(10/8) BsmBI 229 CGTCTC CGTCTC(1/5) BsrBI 28 GAGCGG CCGCTC(-3/-3) BsrDI 431 GCAATG GCAATG(2/0) BstBI 955 TTCGAA TT^CGAA CelII 209 GCTCAGC GC^T[ACGT]AGC Eam1105I 681 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 381 GCGGG CCCGC(4/6) HincII 694 GTCAAC GT[CT]^[GA]AC HindII 694 GTCAAC GT[CT]^[GA]AC HindIII 637 AAGCTT A^AGCTT NciI 563 CCCGG CC^[GC]GG NcoI 755 CCATGG C^CATGG NspI 195 GCATGT [GA]CATG^[CT] NspV 955 TTCGAA TT^CGAA PflFI 612 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 16 CTGCAG CTGCA^G SacI 505 GAGCTC GAGCT^C ScaI 950 AGTACT AGT^ACT SfaNI 479 GCATC GCATC(5/9) SfuI 955 TTCGAA TT^CGAA SmlI 268 CTCAAG C^T[CT][GA]AG Tth111I 612 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 669 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccII 58 CGCG CG^CG 615 CGCG AccIII 287 TCCGGA T^CCGGA 677 TCCGGA AfaI 111 GTAC GT^AC 951 GTAC AspHI 505 GAGCTC G[AT]GC[AT]^C 556 GAGCAC BalI 83 TGGCCA TGG^CCA 489 TGGCCA BanII 409 GAGCCC G[GA]GC[CT]^C 505 GAGCTC BseAI 287 TCCGGA T^CCGGA 677 TCCGGA BsiHKAI 505 GAGCTC G[AT]GC[AT]^C 556 GAGCAC BsiYI 12 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 773 CCTCCACCAGG BslI 12 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 773 CCTCCACCAGG BsmAI 65 GAGAC GTCTC(1/5) 230 GTCTC BspEI 287 TCCGGA T^CCGGA 677 TCCGGA BspMI 292 ACCTGC ACCTGC(4/8) 377 ACCTGC BstUI 58 CGCG CG^CG 615 CGCG BstXI 492 CCATGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 879 CCATCCTGATGG BstYI 384 GGATCC [GA]^GATC[CT] 422 AGATCC Cac8I 258 GCTGGC GC[ACGT]^[ACGT]GC 487 GCTGGC DdeI 210 CTCAG C^T[ACGT]AG 699 CTCAG DraII 89 AGGACCC [GA]G^G[ACGT]CC[CT] 462 GGGACCC DrdI 91 GACCCTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 724 GACCATGTGGTC DsaI 332 CCACGG C^C[GA][CT]GG 755 CCATGG EaeI 83 TGGCCA [CT]^GGCC[GA] 489 TGGCCA EarI 826 GAAGAG CTCTTC(1/4) 836 GAAGAG EcoNI 12 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 773 CCTCCACCAGG EcoO109I 89 AGGACCC [GA]G^G[ACGT]CC[CT] 462 GGGACCC HgaI 175 GACGC GACGC(5/10) 616 GCGTC Ksp632I 826 GAAGAG CTCTTC(1/4) 836 GAAGAG MaeII 687 ACGT A^CGT 923 ACGT MflI 384 GGATCC [GA]^GATC[CT] 422 AGATCC MluNI 83 TGGCCA TGG^CCA 489 TGGCCA MroI 287 TCCGGA T^CCGGA 677 TCCGGA MscI 83 TGGCCA TGG^CCA 489 TGGCCA MseI 47 TTAA T^TAA 551 TTAA MvnI 58 CGCG CG^CG 615 CGCG PpuMI 89 AGGACCC [GA]G^G[AT]CC[CT] 462 GGGACCC RsaI 111 GTAC GT^AC 951 GTAC SfcI 16 CTGCAG C^T[GA][CT]AG 897 CTACAG TaqI 941 TCGA T^CGA 956 TCGA Tru9I 47 TTAA T^TAA 551 TTAA Tsp45I 610 GTGAC ^GT[GC]AC 848 GTCAC Tsp509I 277 AATT ^AATT 959 AATT TthHB8I 941 TCGA T^CGA 956 TCGA XhoII 384 GGATCC [GA]^GATC[CT] 422 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BmyI BsaJI BsaWI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI StyI TseI TspRI