(Circular) (Six-base) MAPSORT of: pFN21AE1446.seq Check: 4798 from: 1 to: 5680 pFN21AE1446 5680 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3743 5127 5395 1632 Size: 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 2691 4409 2691 Size: 1718 3962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AlwNI CAG_nnn'CTG Cuts at: 3423 4995 3423 Size: 1572 4108 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2963 2989 3215 3367 5476 1493 Size: 1470 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 1470 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 3005 1870 Size: 73 1062 4545 Fragments arranged by size: 4545 1062 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2917 2950 2917 Size: 33 5647 BamHI G'GATC_C Cuts at: 2389 3010 3377 2389 Size: 621 367 4692 Fragments arranged by size: 4692 621 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 3001 4456 618 Size: 325 217 627 354 860 1455 1842 Fragments arranged by size: 1842 1455 860 627 354 325 217 BanII G_rGCy'C Cuts at: 1391 2418 2514 2999 1391 Size: 1027 96 485 4072 Fragments arranged by size: 4072 1027 485 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2681 928 Size: 971 782 3927 Fragments arranged by size: 3927 971 782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2002 2036 3871 3905 1903 Size: 34 65 34 1835 34 3678 Fragments arranged by size: 3678 1835 65 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2945 3572 4782 2945 Size: 627 1210 3843 Fragments arranged by size: 3843 1210 627 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4291 136 Size: 107 122 71 631 605 2619 1525 Fragments arranged by size: 2619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 2215 3097 2215 Size: 882 4798 Bme1580I G_kGCm'C Cuts at: 1790 2144 3741 4897 1790 Size: 354 1597 1156 2573 Fragments arranged by size: 2573 1597 1156 354 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2197 3411 4369 Size: 416 53 83 399 24 780 1214 958 Cuts at: 4369 442 Size: 1753 Fragments arranged by size: 1753 1214 958 780 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2093 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2556 4340 1239 Size: 1317 1784 2579 Fragments arranged by size: 2579 1784 1317 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 3805 4670 4968 2258 Size: 1547 865 298 2970 Fragments arranged by size: 2970 1547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2064 4343 882 Size: 617 565 2279 2219 Fragments arranged by size: 2279 2219 617 565 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2159 2292 2682 4113 4511 4785 4932 5343 2159 Size: 133 390 1431 398 274 147 411 2496 Fragments arranged by size: 2496 1431 411 398 390 274 147 133 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 4800 BseYI C'CCAG_C Cuts at: 2137 4883 5379 2137 Size: 2746 496 2438 Fragments arranged by size: 2746 2438 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2514 2565 3741 3826 4897 2514 Size: 51 1176 85 1071 3297 Fragments arranged by size: 3297 1176 1071 85 51 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 BsmBI CGTCTCn'nnnn_ Cuts at: 2240 2240 Size: 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2418 2514 2565 2999 3741 3826 Size: 399 354 274 96 51 434 742 85 Cuts at: 3826 4897 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 434 399 354 274 96 85 51 BspEI T'CCGG_A Cuts at: 2292 2682 5343 2292 Size: 390 2661 2629 Fragments arranged by size: 2661 2629 390 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2306 2391 3035 844 Size: 1462 85 644 3489 Fragments arranged by size: 3489 1462 644 85 BsrBI CCG'CTC Cuts at: 1394 1823 2035 3567 1394 Size: 429 212 1532 3507 Fragments arranged by size: 3507 1532 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 2443 4175 4349 65 Size: 1183 1195 1732 174 1396 Fragments arranged by size: 1732 1396 1195 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 2961 4484 2961 Size: 1523 4157 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2504 2891 2504 Size: 387 5293 BstYI r'GATC_y Cuts at: 1972 2389 2427 3010 3377 3762 3779 5231 5317 Size: 417 38 583 367 385 17 1452 86 Cuts at: 5317 5329 5675 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 583 417 385 367 346 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2337 2760 513 Size: 552 1272 423 3433 Fragments arranged by size: 3433 1272 552 423 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2473 487 Size: 683 56 1247 3694 Fragments arranged by size: 3694 1247 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3990 4010 3196 Size: 794 20 4866 Fragments arranged by size: 4866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2644 2679 2770 2805 529 Size: 35 2080 35 91 35 3404 Fragments arranged by size: 3404 2080 91 35 35 35 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2102 2735 4687 809 Size: 507 786 633 1952 1802 Fragments arranged by size: 1952 1802 786 633 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2494 3127 Size: 54 1425 50 275 18 258 406 633 Cuts at: 3127 4016 5645 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 633 406 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 2836 3610 1353 Size: 1473 10 774 3423 Fragments arranged by size: 3423 1473 774 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2512 2512 Size: 5680 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2556 3743 4340 5127 5395 1239 Size: 393 924 1187 597 787 268 1524 Fragments arranged by size: 1524 1187 924 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2021 2782 2021 Size: 761 4919 EcoO109I rG'GnC_Cy Cuts at: 2095 2468 2095 Size: 373 5307 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2872 2872 Size: 5680 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 4827 1678 Size: 3149 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2677 2709 4321 4353 4395 Size: 32 135 32 1105 32 1612 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 1612 1105 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2701 3024 3270 669 Size: 988 281 763 323 246 3079 Fragments arranged by size: 3079 988 763 323 281 246 HindIII A'AGCT_T Cuts at: 748 2642 748 Size: 1894 3786 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 2496 10 Size: 54 1425 343 258 406 3194 Fragments arranged by size: 3194 1425 406 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2327 2502 2606 2612 2889 3638 Size: 648 981 180 175 104 6 277 749 Cuts at: 3638 3997 4156 518 Size: 359 159 2042 Fragments arranged by size: 2042 981 749 648 359 277 180 175 159 104 6 MspA1I CmG'CkG Cuts at: 1606 2219 2229 2263 2708 2910 3096 3773 4921 Size: 613 10 34 445 202 186 677 1148 Cuts at: 4921 5166 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 613 445 245 202 186 34 10 NcoI C'CATG_G Cuts at: 513 1065 2760 513 Size: 552 1695 3433 Fragments arranged by size: 3433 1695 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2204 3038 4583 1132 Size: 97 975 834 1545 2229 Fragments arranged by size: 2229 1545 975 834 97 PasI CC'CwG_GG Cuts at: 1348 2473 1348 Size: 1125 4555 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2792 2824 3749 3781 5262 5294 2792 Size: 32 925 32 1481 32 3178 Fragments arranged by size: 3178 1481 925 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2468 2095 Size: 373 5307 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2025 3032 830 Size: 1195 1007 3478 Fragments arranged by size: 3478 1195 1007 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2219 2229 2263 2708 2219 Size: 10 34 445 5191 Fragments arranged by size: 5191 445 34 10 SacI G_AGCT'C Cuts at: 2514 2514 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SanDI GG'GwC_CC Cuts at: 2468 2468 Size: 5680 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 2957 3928 5640 2957 Size: 971 1712 2997 Fragments arranged by size: 2997 1712 971 SfcI C'TryA_G Cuts at: 826 1045 2021 2902 3028 4163 4844 5035 826 Size: 219 976 881 126 1135 681 191 1471 Fragments arranged by size: 1471 1135 976 881 681 219 191 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 3784 4685 4947 820 Size: 197 926 330 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 330 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2091 2625 2760 3119 513 Size: 552 1026 534 135 359 3074 Fragments arranged by size: 3074 1026 552 534 359 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3707 3724 3877 4062 4507 325 Size: 1255 2127 17 153 185 445 1498 Fragments arranged by size: 2127 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2955 3926 5638 Size: 274 80 33 51 1443 978 971 1712 Cuts at: 5638 96 Size: 138 Fragments arranged by size: 1712 1443 978 971 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 2620 1316 Size: 1304 4376 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 2678 3809 2678 Size: 1131 4549 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI