Restriction map for pFN21AE1446
 (Linear) (Six-base) MAPSORT of: KIEE1446.seq  Check: 3856  from: 1  to: 975



KIEE1446.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     686     975 
   Size:        686     289
 
ApoI r'AATT_y

Cuts at:      0     958     975 
   Size:        958      17
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     912     945     975 
   Size:        912      33      30

  Fragments arranged by size: 

                 912      33      30
 
BamHI G'GATC_C

Cuts at:      0     384     975 
   Size:        384     591
 
BanI G'GyrC_C

Cuts at:      0     136     975 
   Size:        136     839
 
BanII G_rGCy'C

Cuts at:      0     413     509     975 
   Size:        413      96     466

  Fragments arranged by size: 

                 466     413      96
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     676     975 
   Size:        676     299
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      31     975 
   Size:         31     944
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     940     975 
   Size:        940      35
 
BlpI GC'TnA_GC

Cuts at:      0     210     975 
   Size:        210     765
 
Bme1580I G_kGCm'C

Cuts at:      0     139     975 
   Size:        139     836
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     192     975 
   Size:        192     783
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      88     975 
   Size:         88     887
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     551     975 
   Size:        551     424
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     975 
   Size:        253     722
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      59     975 
   Size:         59     916
 
BsaWI w'CCGG_w

Cuts at:      0     154     287     677     975 
   Size:        154     133     390     298

  Fragments arranged by size: 

                 390     298     154     133
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     975 
   Size:         61     914
 
BseYI C'CCAG_C

Cuts at:      0     132     975 
   Size:        132     843
 
BsiHKAI G_wGCw'C

Cuts at:      0     509     560     975 
   Size:        509      51     415

  Fragments arranged by size: 

                 509     415      51
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     235     975 
   Size:        235     740
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     413     509     560     975 
   Size:        139     274      96      51     415

  Fragments arranged by size: 

                 415     274     139      96      51
 
BspEI T'CCGG_A

Cuts at:      0     287     677     975 
   Size:        287     390     298

  Fragments arranged by size: 

                 390     298     287
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     301     386     975 
   Size:        301      85     589

  Fragments arranged by size: 

                 589     301      85
 
BsrBI CCG'CTC

Cuts at:      0      30     975 
   Size:         30     945
 
BsrDI GCAATG_nn'

Cuts at:      0     438     975 
   Size:        438     537
 
BstBI TT'CG_AA

Cuts at:      0     956     975 
   Size:        956      19
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     499     886     975 
   Size:        499     387      89

  Fragments arranged by size: 

                 499     387      89
 
BstYI r'GATC_y

Cuts at:      0     384     422     975 
   Size:        384      38     553

  Fragments arranged by size: 

                 553     384      38
 
BtgI C'CryG_G

Cuts at:      0     332     755     975 
   Size:        332     423     220

  Fragments arranged by size: 

                 423     332     220
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     468     975 
   Size:        468     507
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     639     674     765     800     975 
   Size:        639      35      91      35     175

  Fragments arranged by size: 

                 639     175      91      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      97     730     975 
   Size:         97     633     245

  Fragments arranged by size: 

                 633     245      97
 
EaeI y'GGCC_r

Cuts at:      0      83     489     975 
   Size:         83     406     486

  Fragments arranged by size: 

                 486     406      83
 
EarI CTCTTCn'nnn_

Cuts at:      0     821     831     975 
   Size:        821      10     144

  Fragments arranged by size: 

                 821     144      10
 
EcoICRI GAG'CTC

Cuts at:      0     507     975 
   Size:        507     468
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     551     975 
   Size:        551     424
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      16     777     975 
   Size:         16     761     198

  Fragments arranged by size: 

                 761     198      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     463     975 
   Size:         90     373     512

  Fragments arranged by size: 

                 512     373      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     867     975 
   Size:        867     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     672     704     975 
   Size:        672      32     271

  Fragments arranged by size: 

                 672     271      32
 
HincII GTy'rAC

Cuts at:      0     696     975 
   Size:        696     279
 
HindIII A'AGCT_T

Cuts at:      0     637     975 
   Size:        637     338
 
MscI TGG'CCA

Cuts at:      0      85     491     975 
   Size:         85     406     484

  Fragments arranged by size: 

                 484     406      85
 
MslI CAynn'nnrTG

Cuts at:      0     142     322     497     601     607     884     975 
   Size:        142     180     175     104       6     277      91

  Fragments arranged by size: 

                 277     180     175     142     104      91       6
 
MspA1I CmG'CkG

Cuts at:      0     214     224     258     703     905     975 
   Size:        214      10      34     445     202      70

  Fragments arranged by size: 

                 445     214     202      70      34      10
 
NcoI C'CATG_G

Cuts at:      0     755     975 
   Size:        755     220
 
NspI r_CATG'y

Cuts at:      0     199     975 
   Size:        199     776
 
PasI CC'CwG_GG

Cuts at:      0     468     975 
   Size:        468     507
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     787     819     975 
   Size:        787      32     156

  Fragments arranged by size: 

                 787     156      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      90     463     975 
   Size:         90     373     512

  Fragments arranged by size: 

                 512     373      90
 
PstI C_TGCA'G

Cuts at:      0      20     975 
   Size:         20     955
 
PvuII CAG'CTG

Cuts at:      0     214     224     258     703     975 
   Size:        214      10      34     445     272

  Fragments arranged by size: 

                 445     272     214      34      10
 
SacI G_AGCT'C

Cuts at:      0     509     975 
   Size:        509     466
 
SanDI GG'GwC_CC

Cuts at:      0     463     975 
   Size:        463     512
 
ScaI AGT'ACT

Cuts at:      0     952     975 
   Size:        952      23
 
SfcI C'TryA_G

Cuts at:      0      16     897     975 
   Size:         16     881      78

  Fragments arranged by size: 

                 881      78      16
 
SmlI C'TyrA_G

Cuts at:      0     268     975 
   Size:        268     707
 
StyI C'CwwG_G

Cuts at:      0      86     620     755     975 
   Size:         86     534     135     220

  Fragments arranged by size: 

                 534     220     135      86
 
TatI w'GTAC_w

Cuts at:      0     950     975 
   Size:        950      25
 
Tth111I GACn'n_nGTC

Cuts at:      0     615     975 
   Size:        615     360
 
XmnI GAAnn'nnTTC

Cuts at:      0     673     975 
   Size:        673     302
 

 Enzymes that do cut:

     AhdI     ApoI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaWI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BstBI    BstXI    BstYI     BtgI    BtgZI    CspCI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I   HincII  HindIII     MscI     MslI   MspA1I     NcoI     NspI
     PasI     PpiI    PpuMI     PstI    PvuII     SacI    SanDI     ScaI
     SfcI     SmlI     StyI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BbeI    BbvCI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BspHI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I     BtsI
     ClaI     DraI   DraIII     EagI     EciI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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