Restriction map for
pFN21AE1445
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI HgaI Hin1I
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MaeII MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AlwI 94 GATCC GGATC(4/5)
AspHI 3 GAGCTC G[AT]GC[AT]^C
AvaI 12 CTCGGG C^[CT]CG[GA]G
AvaII 117 GGACC G^G[AT]CC
AviII 202 TGCGCA TGC^GCA
BbsI 349 GTCTTC GAAGAC(2/6)
BfaI 241 CTAG C^TAG
BglI 183 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 237 AGATCT A^GATCT
BpmI 605 CTGGAG CTGGAG(16/14)
BpuAI 349 GTCTTC GAAGAC(2/6)
BsaBI 468 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsoBI 12 CTCGGG C^[CT]CG[GA]G
BstNI 450 CCTGG CC^[AT]GG
BstYI 237 AGATCT [GA]^GATC[CT]
Cac8I 600 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 117 GGACC G^G[ACGT]CC
DraIII 502 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 360 CCATGG C^C[GA][CT]GG
EcoRII 450 CCTGG ^CC[AT]GG
EcoT14I 360 CCATGG C^C[AT][AT]GG
EcoT22I 383 ATGCAT ATGCA^T
FspI 202 TGCGCA TGC^GCA
HaeII 773 AGCGCC [GA]GCGC^[CT]
HaeIII 9 GGCC GG^CC
HincII 309 GTCAAC GT[CT]^[GA]AC
HindII 309 GTCAAC GT[CT]^[GA]AC
MaeI 241 CTAG C^TAG
MamI 468 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 806 CAATTG C^AATTG
MflI 237 AGATCT [GA]^GATC[CT]
MunI 806 CAATTG C^AATTG
MvaI 450 CCTGG CC^[AT]GG
NcoI 360 CCATGG C^CATGG
NdeI 386 CATATG CA^TATG
NsiI 383 ATGCAT ATGCA^T
NspI 484 GCATGT [GA]CATG^[CT]
PshAI 436 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3 GAGCTC GAGCT^C
Sau96I 117 GGACC G^G[ACGT]CC
StyI 360 CCATGG C^C[AT][AT]GG
TaqI 401 TCGA T^CGA
Tsp45I 443 GTCAC ^GT[GC]AC
TthHB8I 401 TCGA T^CGA
XhoII 237 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 296 AAATTC [GA]^AATT[CT]
404 AAATTC
AfaI 108 GTAC GT^AC
681 GTAC
ApoI 296 AAATTC [GA]^AATT[CT]
404 AAATTC
BanI 29 GGCACC G^G[CT][GA]CC
196 GGCACC
BanII 3 GAGCTC G[GA]GC[CT]^C
16 GGGCTC
BcnI 33 CCCGG CC^[GC]GG
657 CCGGG
BsaJI 11 CCTCGG C^C[ACGT][ACGT]GG
360 CCATGG
BsiYI 34 CCGGCAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
744 CCTCACACTGG
BslI 34 CCGGCAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
744 CCTCACACTGG
BsmAI 212 GAGAC GTCTC(1/5)
434 GAGAC
BspHI 153 TCATGA T^CATGA
300 TCATGA
BstAPI 385 GCATATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
501 GCACGATGTGC
BstF5I 525 GGATG GGATG(2/0)
576 GGATG
CfoI 203 GCGC GCG^C
774 GCGC
FauI 224 CCCGC CCCGC(4/6)
192 GCGGG
FokI 525 GGATG GGATG(9/13)
576 GGATG
HhaI 203 GCGC GCG^C
774 GCGC
HinP1I 203 GCGC G^CGC
774 GCGC
HphI 753 GGTGA GGTGA(8/7)
654 TCACC
MaeIII 443 GTCAC ^GT[ACGT]AC
626 GTTAC
NciI 33 CCCGG CC^[GC]GG
657 CCGGG
RcaI 153 TCATGA T^CATGA
300 TCATGA
RsaI 108 GTAC GT^AC
681 GTAC
TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^
749 CACTG
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BmyI BseRI
Bsp1286I BspMI BsrI BssKI DdeI
DpnII Fnu4HI HapII HpaII ItaI
MboI MboII MnlI MseI MslI
MspI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp509I