Restriction map for
pFN21AE1445
(Circular) (Six-base) MAPSORT of: pFN21AE1445.seq Check: 6589 from: 1 to: 5584
pFN21AE1445 5584 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2213 2033
Size: 180 5404
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3647 5031 5299 1632
Size: 2015 1384 268 1917
Fragments arranged by size:
2015 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 2756 1166
Size: 1590 3994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AlwNI CAG_nnn'CTG
Cuts at: 3327 4899 3327
Size: 1572 4012
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2301 2409 2893 3119 3271 5380 1493
Size: 808 108 484 226 152 2109 1697
Fragments arranged by size:
2109 1697 808 484 226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2909 1870
Size: 73 74 892 4545
Fragments arranged by size:
4545 892 74 73
BamHI G'GATC_C
Cuts at: 2914 3281 2914
Size: 367 5217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2034 2201 2905 4360 618
Size: 325 217 627 247 167 704 1455 1842
Fragments arranged by size:
1842 1455 704 627 325 247 217 167
BanII G_rGCy'C
Cuts at: 1391 2012 2025 2903 1391
Size: 621 13 878 4072
Fragments arranged by size:
4072 878 621 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2347 928
Size: 971 448 4165
Fragments arranged by size:
4165 971 448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3775 3809 1903
Size: 34 1838 34 3678
Fragments arranged by size:
3678 1838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BfrBI ATG'CAT
Cuts at: 2390 2390
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2194 4195 136
Size: 107 122 71 631 605 522 2001 1525
Fragments arranged by size:
2001 1525 631 605 522 122 107 71
BglII A'GATC_T
Cuts at: 2242 5579 2242
Size: 3337 2247
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2204 3645 4801 1790
Size: 247 167 1441 1156 2573
Fragments arranged by size:
2573 1441 1156 247 167
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4273 442
Size: 416 53 83 399 24 1898 958 1753
Fragments arranged by size:
1898 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2631 4244 1239
Size: 1392 1613 2579
Fragments arranged by size:
2579 1613 1392
Bpu10I CC'TnA_GC
Cuts at: 2360 2360
Size: 5584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3375 493
Size: 711 2171 2702
Fragments arranged by size:
2702 2171 711
BsaBI GATnn'nnATC
Cuts at: 2477 5578 2477
Size: 3101 2483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 3773 4408 275
Size: 53 83 186 936 1858 382 635 1451
Fragments arranged by size:
1858 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2716 2738 1186
Size: 960 570 22 4032
Fragments arranged by size:
4032 960 570 22
BseYI C'CCAG_C
Cuts at: 4787 5283 4787
Size: 496 5088
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5011
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3795 4823 1540
Size: 275 186 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3645 3730 4801 2012
Size: 1633 85 1071 2795
Fragments arranged by size:
2795 1633 1071 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2025 2037 2204 2903 3645 3730
Size: 399 222 13 12 167 699 742 85
Cuts at: 3730 4801 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 699 399 222 167 85
13 12
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 2158 2305 3473 5203 2158
Size: 147 1168 1730 2539
Fragments arranged by size:
2539 1730 1168 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 2213 2223 2815 2939 844
Size: 1189 180 10 592 124 3489
Fragments arranged by size:
3489 1189 592 180 124 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2396 2512 794
Size: 1602 116 3866
Fragments arranged by size:
3866 1602 116
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2242 2914 3281 3666 3683 5135 5221 5233
Size: 270 672 367 385 17 1452 86 12
Cuts at: 5233 5579 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 672 385 367 346 270 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2365 513
Size: 552 1300 3732
Fragments arranged by size:
3732 1300 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2183 3100 3894 3914 2183
Size: 917 794 20 3853
Fragments arranged by size:
3853 917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3920 5549
Size: 54 1425 50 275 18 1201 889 1629
Cuts at: 5549 8
Size: 43
Fragments arranged by size:
1629 1425 1201 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3514 1353
Size: 2161 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2631 3647 4244 5031 5299 1239
Size: 393 999 1016 597 787 268 1524
Fragments arranged by size:
1524 1016 999 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 2209 4090 2209
Size: 1881 3703
HaeII r_GCGC'y
Cuts at: 1678 2782 4731 1678
Size: 1104 1949 2531
Fragments arranged by size:
2531 1949 1104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 4225 4257 4299
Size: 32 135 32 1001 32 1620 32 42
Cuts at: 4299 4331 1373
Size: 32 2626
Fragments arranged by size:
2626 1620 1001 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2316 2928 3174 669
Size: 988 281 378 612 246 3079
Fragments arranged by size:
3079 988 612 378 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 2811 3183 2811
Size: 372 5212
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2440 2538 4698 4882 1305
Size: 1135 98 2160 184 2007
Fragments arranged by size:
2160 2007 1135 184 98
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3762
Fragments arranged by size:
3762 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 2385 2756 3542 3901 4060 518
Size: 648 1165 54 371 786 359 159 2042
Fragments arranged by size:
2042 1165 786 648 371 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 3000 3677 4825 5070 1606
Size: 1394 677 1148 245 2120
Fragments arranged by size:
2120 1394 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2365 513
Size: 552 1300 3732
Fragments arranged by size:
3732 1300 552
NdeI CA'TA_TG
Cuts at: 387 2392 387
Size: 2005 3579
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NsiI A_TGCA'T
Cuts at: 2392 2392
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2493 2942 4487 1132
Size: 97 1264 449 1545 2229
Fragments arranged by size:
2229 1545 1264 449 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2280 2312 3653 3685 5166 5198 2280
Size: 32 1341 32 1481 32 2666
Fragments arranged by size:
2666 1481 1341 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2445 1613
Size: 832 4752
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2936 830
Size: 2106 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2932 4067 4748 4939 826
Size: 219 1887 1135 681 191 1471
Fragments arranged by size:
1887 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2911 1872
Size: 1039 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 2365 3023 513
Size: 552 1300 658 3074
Fragments arranged by size:
3074 1300 658 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3611 3628 3781 3966 4411 325
Size: 1255 2031 17 153 185 445 1498
Fragments arranged by size:
2031 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3830 5542
Size: 274 80 33 51 1443 135 1718 1712
Cuts at: 5542 96
Size: 138
Fragments arranged by size:
1718 1712 1443 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2241 2273 5544 5576 2241
Size: 32 3271 32 2249
Fragments arranged by size:
3271 2249 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2909 1870
Size: 1039 4545
XmnI GAAnn'nnTTC
Cuts at: 3713 3713
Size: 5584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI