(Circular) (Six-base) MAPSORT of: pFN21AB4458.seq Check: 8377 from: 1 to: 8335 pFN21AB4458 8335 bp With 182 enzymes: SgfI * March 2, 2009 15:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2801 2882 3722 2801 Size: 81 840 7414 Fragments arranged by size: 7414 840 81 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6145 278 Size: 53 83 186 936 4609 2468 Fragments arranged by size: 4609 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4833 5678 1937 Size: 2896 845 4594 Fragments arranged by size: 4594 2896 845 Acc65I G'GTAC_C Cuts at: 5656 5656 Size: 8335 AclI AA'CG_TT Cuts at: 1563 6462 6835 1563 Size: 4899 373 3063 Fragments arranged by size: 4899 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 5059 6398 7782 8050 1632 Size: 788 2639 1339 1384 268 1917 Fragments arranged by size: 2639 1917 1384 1339 788 268 AfeI AGC'GCT Cuts at: 2034 4934 2034 Size: 2900 5435 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8138 AflIII A'CryG_T Cuts at: 1203 2529 4323 7234 8211 1203 Size: 1326 1794 2911 977 1327 Fragments arranged by size: 2911 1794 1327 1326 977 AgeI A'CCGG_T Cuts at: 7166 7166 Size: 8335 AhdI GACnn_n'nnGTC Cuts at: 3456 7064 3456 Size: 3608 4727 AleI CACnn'nnGTG Cuts at: 1166 2876 4686 5399 1166 Size: 1710 1810 713 4102 Fragments arranged by size: 4102 1810 1710 713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8335 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 3326 Size: 32 8303 AlwNI CAG_nnn'CTG Cuts at: 2632 2694 3178 4444 5273 5347 6078 7650 2632 Size: 62 484 1266 829 74 731 1572 3317 Fragments arranged by size: 3317 1572 1266 829 731 484 74 62 ApaI G_GGCC'C Cuts at: 3557 3964 5479 3557 Size: 407 1515 6413 Fragments arranged by size: 6413 1515 407 ApaLI G'TGCA_C Cuts at: 4505 6392 7548 4505 Size: 1887 1156 5292 Fragments arranged by size: 5292 1887 1156 ApoI r'AATT_y Cuts at: 1493 2194 2298 2347 2419 2762 3035 3406 4136 Size: 701 104 49 72 343 273 371 730 Cuts at: 4136 4221 4904 5621 5644 5870 6022 8131 1493 Size: 85 683 717 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 730 717 701 683 371 343 273 226 152 104 85 72 49 23 AseI AT'TA_AT Cuts at: 160 1032 6889 160 Size: 872 5857 1606 Fragments arranged by size: 5857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 AvaI C'yCGr_G Cuts at: 1870 1943 2733 2846 3539 4920 5411 5660 1870 Size: 73 790 113 693 1381 491 249 4545 Fragments arranged by size: 4545 1381 790 693 491 249 113 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3724 3757 3724 Size: 33 8302 BamHI G'GATC_C Cuts at: 3166 4197 4736 5665 6032 3166 Size: 1031 539 929 367 5469 Fragments arranged by size: 5469 1031 929 539 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 2867 4014 5228 5656 Size: 325 217 627 654 426 1147 1214 428 Cuts at: 5656 7111 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1214 1147 654 627 428 426 325 217 BanII G_rGCy'C Cuts at: 1391 2077 3557 3564 3964 4163 5114 5479 5573 Size: 686 1480 7 400 199 951 365 94 Cuts at: 5573 5654 1391 Size: 81 4072 Fragments arranged by size: 4072 1480 951 686 400 365 199 94 81 7 BbeI G_GCGC'C Cuts at: 2445 4018 5232 2445 Size: 1573 1214 5548 Fragments arranged by size: 5548 1573 1214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 5025 5196 928 Size: 971 1071 2055 171 4067 Fragments arranged by size: 4067 2055 1071 971 171 BbvCI CC'TCA_GC Cuts at: 4806 4806 Size: 8335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5447 5481 6526 6560 1903 Size: 34 50 34 3426 34 1045 34 3678 Fragments arranged by size: 3678 3426 1045 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 6227 7437 3752 Size: 2475 1210 4650 Fragments arranged by size: 4650 2475 1210 BclI T'GATC_A Cuts at: 1548 4839 4896 1548 Size: 3291 57 4987 Fragments arranged by size: 4987 3291 57 BfrBI ATG'CAT Cuts at: 4966 4966 Size: 8335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2939 4338 5474 Size: 107 122 71 631 605 1267 1399 1136 Cuts at: 5474 6946 136 Size: 1472 1525 Fragments arranged by size: 1525 1472 1399 1267 1136 631 605 122 107 71 BglII A'GATC_T Cuts at: 4770 8330 4770 Size: 3560 4775 BlpI GC'TnA_GC Cuts at: 5752 5752 Size: 8335 Bme1580I G_kGCm'C Cuts at: 1790 2132 2653 3557 3754 3964 4509 5005 5479 Size: 342 521 904 197 210 545 496 474 Cuts at: 5479 6396 7552 1790 Size: 917 1156 2573 Fragments arranged by size: 2573 1156 917 904 545 521 496 474 342 210 197 BmgBI CAC'GTC Cuts at: 1318 5032 5091 1318 Size: 3714 59 4562 Fragments arranged by size: 4562 3714 59 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 3957 4080 Size: 416 53 83 399 24 1265 1275 123 Cuts at: 4080 4432 5261 5482 6066 7024 442 Size: 352 829 221 584 958 1753 Fragments arranged by size: 1753 1275 1265 958 829 584 416 399 352 221 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2091 4792 4817 2091 Size: 2701 25 5609 Fragments arranged by size: 5609 2701 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2141 3650 4717 4805 6995 1239 Size: 860 42 1509 1067 88 2190 2579 Fragments arranged by size: 2579 2190 1509 1067 860 88 42 Bpu10I CC'TnA_GC Cuts at: 2359 4806 5108 2359 Size: 2447 302 5586 Fragments arranged by size: 5586 2447 302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3347 4135 6460 7325 7623 3347 Size: 788 2325 865 298 4059 Fragments arranged by size: 4059 2325 865 788 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4917 5361 6998 882 Size: 617 3418 444 1637 2219 Fragments arranged by size: 3418 2219 1637 617 444 BsaAI yAC'GTr Cuts at: 493 1204 2781 2826 4297 4324 6126 493 Size: 711 1577 45 1471 27 1802 2702 Fragments arranged by size: 2702 1802 1577 1471 711 45 27 BsaBI GATnn'nnATC Cuts at: 8329 8329 Size: 8335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 2970 3840 4015 Size: 53 83 186 936 909 528 870 175 Cuts at: 4015 5229 6142 6524 7159 275 Size: 1214 913 382 635 1451 Fragments arranged by size: 1451 1214 936 913 909 870 635 528 382 186 175 83 53 BsaWI w'CCGG_w Cuts at: 2393 3455 4163 6768 7166 7440 7587 7998 2393 Size: 1062 708 2605 398 274 147 411 2730 Fragments arranged by size: 2730 2605 1062 708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139 Size: 30 165 30 1632 30 12 30 32 Cuts at: 3139 3169 4614 4644 1178 Size: 30 1445 30 4869 Fragments arranged by size: 4869 1632 1445 165 32 30 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 3147 3150 3956 4765 1186 Size: 1444 517 3 806 809 4756 Fragments arranged by size: 4756 1444 809 806 517 3 BseYI C'CCAG_C Cuts at: 2601 2652 3549 4156 4336 4588 4929 5360 7538 Size: 51 897 607 180 252 341 431 2178 Cuts at: 7538 8034 2601 Size: 496 2902 Fragments arranged by size: 2902 2178 897 607 496 431 341 252 180 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2628 2707 3073 3700 3859 4481 Size: 573 498 394 79 366 627 159 622 Cuts at: 4481 5339 1163 Size: 858 4159 Fragments arranged by size: 4159 858 627 622 573 498 394 366 159 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 5785 6546 7574 1540 Size: 275 186 24 3760 761 1028 2301 Fragments arranged by size: 3760 2301 1028 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2663 3536 4509 5114 6396 6481 7552 2663 Size: 873 973 605 1282 85 1071 3446 Fragments arranged by size: 3446 1282 1071 973 873 605 85 BsmI GAATG_Cn' Cuts at: 3611 4968 5846 5939 3611 Size: 1357 878 93 6007 Fragments arranged by size: 6007 1357 878 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2763 3580 2763 Size: 817 7518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2132 2653 2663 3536 3557 3564 Size: 399 287 55 521 10 873 21 7 Cuts at: 3564 3754 3964 4163 4509 5005 5114 5479 5573 Size: 190 210 199 346 496 109 365 94 Cuts at: 5573 5654 6396 6481 7552 1391 Size: 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 873 742 521 496 399 365 346 287 210 199 190 109 94 85 81 55 21 10 7 BspEI T'CCGG_A Cuts at: 7998 7998 Size: 8335 BspHI T'CATG_A Cuts at: 6224 7954 6224 Size: 1730 6605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 2801 2882 3722 3794 4452 5690 844 Size: 1478 479 81 840 72 658 1238 3489 Fragments arranged by size: 3489 1478 1238 840 658 479 81 72 BsrBI CCG'CTC Cuts at: 1394 1823 6222 1394 Size: 429 4399 3507 Fragments arranged by size: 4399 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 6830 7004 65 Size: 1183 1026 4556 174 1396 Fragments arranged by size: 4556 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3121 3842 6979 7166 1700 Size: 110 1311 721 3137 187 2869 Fragments arranged by size: 3137 2869 1311 721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8335 BssSI C'ACGA_G Cuts at: 2104 2920 6395 7407 2104 Size: 816 3475 1012 3032 Fragments arranged by size: 3475 3032 1012 816 BstAPI GCAn_nnn'nTGC Cuts at: 794 3606 3675 4381 794 Size: 2812 69 706 4748 Fragments arranged by size: 4748 2812 706 69 BstBI TT'CG_AA Cuts at: 7139 7139 Size: 8335 BstEII G'GTnAC_C Cuts at: 1184 4708 1184 Size: 3524 4811 BstXI CCAn_nnnn'nTGG Cuts at: 3952 4588 3952 Size: 636 7699 BstYI r'GATC_y Cuts at: 1972 3166 3930 4119 4197 4736 4770 5665 6032 Size: 1194 764 189 78 539 34 895 367 Cuts at: 6032 6417 6434 7886 7972 7984 8330 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1194 895 764 539 385 367 346 189 86 78 34 17 12 Bsu36I CC'TnA_GG Cuts at: 3696 5597 3696 Size: 1901 6434 BtgI C'CryG_G Cuts at: 513 1065 2674 5142 513 Size: 552 1609 2468 3706 Fragments arranged by size: 3706 2468 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3925 487 Size: 683 56 2699 4897 Fragments arranged by size: 4897 2699 683 56 BtsI GCAGTG_nn' Cuts at: 2518 2663 3772 5394 5406 5623 5851 6645 6665 Size: 145 1109 1622 12 217 228 794 20 Cuts at: 6665 2518 Size: 4188 Fragments arranged by size: 4188 1622 1109 794 228 217 145 20 12 ClaI AT'CG_AT Cuts at: 1554 4827 1554 Size: 3273 5062 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8300 DraI TTT'AAA Cuts at: 3040 5639 3040 Size: 2599 5736 DraIII CAC_nnn'GTG Cuts at: 4088 4088 Size: 8335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 7342 809 Size: 507 1434 4592 1802 Fragments arranged by size: 4592 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3844 4302 Size: 54 1425 50 275 18 192 1822 458 Cuts at: 4302 4578 5782 6671 8300 8 Size: 276 1204 889 1629 43 Fragments arranged by size: 1822 1629 1425 1204 889 458 276 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 5782 1537 Size: 275 210 3760 4090 Fragments arranged by size: 4090 3760 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 4007 4593 6265 1353 Size: 2654 586 1672 3423 Fragments arranged by size: 3423 2654 1672 586 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6922 7296 7442 387 Size: 1064 138 5333 374 146 1280 Fragments arranged by size: 5333 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5112 5112 Size: 8335 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2141 2420 3650 4717 4805 5059 Size: 393 467 42 279 1230 1067 88 254 Cuts at: 5059 6398 6995 7782 8050 1239 Size: 1339 597 787 268 1524 Fragments arranged by size: 1524 1339 1230 1067 787 597 467 393 279 268 254 88 42 EcoNI CCTnn'n_nnAGG Cuts at: 3629 3695 3629 Size: 66 8269 EcoO109I rG'GnC_Cy Cuts at: 2729 2996 3450 3553 3960 3961 5383 2729 Size: 267 454 103 407 1 1422 5681 Fragments arranged by size: 5681 1422 454 407 267 103 1 EcoRI G'AATT_C Cuts at: 2298 4136 4904 5644 6022 2298 Size: 1838 768 740 378 4611 Fragments arranged by size: 4611 1838 768 740 378 FspI TGC'GCA Cuts at: 6841 6841 Size: 8335 HaeII r_GCGC'y Cuts at: 1678 2036 2045 2445 2671 4018 4936 5232 7482 Size: 358 9 400 226 1347 918 296 2250 Cuts at: 7482 1678 Size: 2531 Fragments arranged by size: 2531 2250 1347 918 400 358 296 226 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 3442 Size: 32 135 32 1331 32 100 32 375 Cuts at: 3442 3474 3560 3592 4182 4214 6976 7008 7050 Size: 32 86 32 590 32 2762 32 42 Cuts at: 7050 7082 1373 Size: 32 2626 Fragments arranged by size: 2762 2626 1331 590 375 135 100 86 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5446 5679 5925 669 Size: 988 281 3508 233 246 3079 Fragments arranged by size: 3508 3079 988 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 8335 HpaI GTT'AAC Cuts at: 5925 5925 Size: 8335 KasI G'GCGC_C Cuts at: 2441 4014 5228 2441 Size: 1573 1214 5548 Fragments arranged by size: 5548 1573 1214 KpnI G_GTAC'C Cuts at: 5660 5660 Size: 8335 MfeI C'AATT_G Cuts at: 5934 5934 Size: 8335 MluI A'CGCG_T Cuts at: 8211 8211 Size: 8335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3938 5013 7449 7633 1305 Size: 2633 1075 2436 184 2007 Fragments arranged by size: 2633 2436 2007 1075 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4304 4580 10 Size: 54 1425 343 2472 276 3765 Fragments arranged by size: 3765 2472 1425 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2876 3950 4686 4816 4841 5399 Size: 648 1368 342 1074 736 130 25 558 Cuts at: 5399 6293 6652 6811 518 Size: 894 359 159 2042 Fragments arranged by size: 2042 1368 1074 894 736 648 558 359 342 159 130 25 MspA1I CmG'CkG Cuts at: 1606 2562 3498 5309 5579 5751 6428 7576 7821 Size: 956 936 1811 270 172 677 1148 245 Cuts at: 7821 1606 Size: 2120 Fragments arranged by size: 2120 1811 1148 956 936 677 270 245 172 NaeI GCC'GGC Cuts at: 3844 3844 Size: 8335 NarI GG'CG_CC Cuts at: 2442 4015 5229 2442 Size: 1573 1214 5548 Fragments arranged by size: 5548 1573 1214 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7783 NdeI CA'TA_TG Cuts at: 387 4747 4817 387 Size: 4360 70 3905 Fragments arranged by size: 4360 3905 70 NgoMIV G'CCGG_C Cuts at: 3842 3842 Size: 8335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8335 NotI GC'GGCC_GC Cuts at: 5782 5782 Size: 8335 NsiI A_TGCA'T Cuts at: 4968 4968 Size: 8335 NspI r_CATG'y Cuts at: 1132 1229 2533 4556 5693 7238 1132 Size: 97 1304 2023 1137 1545 2229 Fragments arranged by size: 2229 2023 1545 1304 1137 97 PasI CC'CwG_GG Cuts at: 1348 3988 1348 Size: 2640 5695 PciI A'CATG_T Cuts at: 2529 7234 2529 Size: 4705 3630 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 3920 1108 Size: 1149 1663 5523 Fragments arranged by size: 5523 1663 1149 PfoI T'CCnGG_A Cuts at: 2015 2250 4398 4410 4782 2015 Size: 235 2148 12 372 5568 Fragments arranged by size: 5568 2148 372 235 12 PmeI GTTT'AAAC Cuts at: 5639 5639 Size: 8335 PmlI CAC'GTG Cuts at: 2781 4324 2781 Size: 1543 6792 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6404 6436 7917 7949 6404 Size: 32 1481 32 6790 Fragments arranged by size: 6790 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3450 5383 3450 Size: 1933 6402 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8335 PsiI TTA'TAA Cuts at: 3476 5905 3476 Size: 2429 5906 PspOMI G'GGCC_C Cuts at: 3553 3960 5475 3553 Size: 407 1515 6413 Fragments arranged by size: 6413 1515 407 PstI C_TGCA'G Cuts at: 830 2319 2719 4056 5401 5687 830 Size: 1489 400 1337 1345 286 3478 Fragments arranged by size: 3478 1489 1345 1337 400 286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8335 PvuII CAG'CTG Cuts at: 2562 3498 5579 2562 Size: 936 2081 5318 Fragments arranged by size: 5318 2081 936 SacI G_AGCT'C Cuts at: 5114 5114 Size: 8335 SalI G'TCGA_C Cuts at: 1936 5677 1936 Size: 3741 4594 SbfI CC_TGCA'GG Cuts at: 5687 5687 Size: 8335 ScaI AGT'ACT Cuts at: 6583 8295 6583 Size: 1712 6623 SfcI C'TryA_G Cuts at: 826 1045 2315 2715 4052 4980 5397 5683 6818 Size: 219 1270 400 1337 928 417 286 1135 Cuts at: 6818 7499 7690 826 Size: 681 191 1471 Fragments arranged by size: 1471 1337 1270 1135 928 681 417 400 286 219 191 SfoI GGC'GCC Cuts at: 2443 4016 5230 2443 Size: 1573 1214 5548 Fragments arranged by size: 5548 1573 1214 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8335 SmaI CCC'GGG Cuts at: 1872 2735 5662 1872 Size: 863 2927 4545 Fragments arranged by size: 4545 2927 863 SmlI C'TyrA_G Cuts at: 820 1017 1943 3362 4114 6439 7340 7602 820 Size: 197 926 1419 752 2325 901 262 1553 Fragments arranged by size: 2325 1553 1419 926 901 752 262 197 SnaBI TAC'GTA Cuts at: 493 6126 493 Size: 5633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8335 SphI G_CATG'C Cuts at: 1132 5693 1132 Size: 4561 3774 SspI AAT'ATT Cuts at: 5 52 3070 4429 6259 5 Size: 47 3018 1359 1830 2081 Fragments arranged by size: 3018 2081 1830 1359 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8335 StyI C'CwwG_G Cuts at: 513 1065 2098 2188 3345 3564 3641 5538 5774 Size: 552 1033 90 1157 219 77 1897 236 Cuts at: 5774 513 Size: 3074 Fragments arranged by size: 3074 1897 1157 1033 552 236 219 90 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3696 4099 4580 4828 6362 6379 6532 Size: 1255 2116 403 481 248 1534 17 153 Cuts at: 6532 6717 7162 325 Size: 185 445 1498 Fragments arranged by size: 2116 1534 1498 1255 481 445 403 248 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 6581 8293 Size: 274 80 33 51 1443 360 4244 1712 Cuts at: 8293 96 Size: 138 Fragments arranged by size: 4244 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3139 3171 4614 4646 8295 8327 3139 Size: 32 1443 32 3649 32 3147 Fragments arranged by size: 3649 3147 1443 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2504 2969 3572 4406 1316 Size: 1188 465 603 834 5245 Fragments arranged by size: 5245 1188 834 603 465 XbaI T'CTAG_A Cuts at: 5671 5671 Size: 8335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2258 2830 3952 4531 2254 Size: 4 572 1122 579 6058 Fragments arranged by size: 6058 1122 579 572 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8335 XmaI C'CCGG_G Cuts at: 1870 2733 5660 1870 Size: 863 2927 4545 Fragments arranged by size: 4545 2927 863 XmnI GAAnn'nnTTC Cuts at: 6464 6464 Size: 8335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6143 276 Size: 53 83 186 936 4609 2468 Fragments arranged by size: 4609 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BsiWI BstZ17I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI