Restriction map for
pFN21AE3662
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbvCI BciVI
BfrI BglII BlnI BseAI BsiWI
BspDI BspEI BssHII BstBI ClaI
CpoI DraI DrdI EcoT22I FseI
FspI HindIII HpaI KpnI MfeI
MluI MroI MunI NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TfiI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 1567 GAATTC [GA]^AATT[CT]
AhdI 955 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 500 AGCGCT AGC^GCT
ApaI 2093 GGGCCC GGGCC^C
ApoI 1567 GAATTC [GA]^AATT[CT]
AspEI 955 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2918 GGATCC G^GATCC
BbeI 331 GGCGCC GGCGC^C
BbrPI 478 CACGTG CAC^GTG
BclI 2066 TGATCA T^GATCA
BfaI 2032 CTAG C^TAG
BsaI 637 GAGACC GGTCTC(1/5)
BsaWI 2372 ACCGGA [AT]^CCGG[AT]
BsmI 1123 GAATGC GAATGC(1/-1)
Bsp1407I 1880 TGTACA T^GTACA
BspLU11I 194 ACATGT A^CATGT
BspMI 1093 ACCTGC ACCTGC(4/8)
BsrGI 1880 TGTACA T^GTACA
Bst1107I 2760 GTATAC GTA^TAC
BstAPI 885 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1263 GGTAACC G^GT[ACGT]ACC
BstPI 1263 GGTAACC G^GT[ACGT]ACC
BstZ17I 2760 GTATAC GTA^TAC
DraIII 2470 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 955 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 721 GAAGAG CTCTTC(1/4)
Eco47III 500 AGCGCT AGC^GCT
EcoO65I 1263 GGTAACC G^GT[ACGT]ACC
EcoRI 1567 GAATTC G^AATTC
EcoRV 550 GATATC GAT^ATC
FbaI 2066 TGATCA T^GATCA
HincII 2239 GTCAAC GT[CT]^[GA]AC
HindII 2239 GTCAAC GT[CT]^[GA]AC
KasI 331 GGCGCC G^GCGCC
Ksp632I 721 GAAGAG CTCTTC(1/4)
MaeI 2032 CTAG C^TAG
MseI 842 TTAA T^TAA
NarI 331 GGCGCC GG^CGCC
NcoI 2516 CCATGG C^CATGG
NdeI 1778 CATATG CA^TATG
NotI 58 GCGGCCGC GC^GGCCGC
PmaCI 478 CACGTG CAC^GTG
PmlI 478 CACGTG CAC^GTG
SapI 721 GAAGAGC GCTCTTC(1/4)
SfiI 669 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 331 GGCGCC GGC^GCC
SspBI 1880 TGTACA T^GTACA
Tru9I 842 TTAA T^TAA
Tsp509I 1568 AATT ^AATT
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 194 ACATGT A^C[GA][CT]GT
2396 ACGTGT
Alw44I 1561 GTGCAC G^TGCAC
2206 GTGCAC
ApaLI 1561 GTGCAC G^TGCAC
2206 GTGCAC
BbsI 1755 GAAGAC GAAGAC(2/6)
1896 GAAGAC
BcgI 458 GCAGCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2228 GCAACATCCTCG
BlpI 271 GCTGAGC GC^T[ACGT]AGC
1353 GCTTAGC
Bpu1102I 271 GCTGAGC GC^T[ACGT]AGC
1353 GCTTAGC
BpuAI 1755 GAAGAC GAAGAC(2/6)
1896 GAAGAC
BsiEI 59 CGGCCG CG[GA][CT]^CG
2780 CGGCCG
BsmBI 1251 CGTCTC CGTCTC(1/5)
1359 CGTCTC
BspHI 173 TCATGA T^CATGA
2546 TCATGA
BsrDI 432 CATTGC GCAATG(2/0)
1244 GCAATG
BssSI 2470 CACGAG CACGAG(-5/-1)
2691 CACGAG
BstXI 1502 CCACCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2510 CCACACCCATGG
CelII 271 GCTGAGC GC^T[ACGT]AGC
1353 GCTTAGC
EagI 59 CGGCCG C^GGCCG
2780 CGGCCG
EclXI 59 CGGCCG C^GGCCG
2780 CGGCCG
Eco52I 59 CGGCCG C^GGCCG
2780 CGGCCG
KspI 56 CCGCGG CCGC^GG
62 CCGCGG
PflMI 696 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2339 CCATCCGCTGG
RcaI 173 TCATGA T^CATGA
2546 TCATGA
SacII 56 CCGCGG CCGC^GG
62 CCGCGG
SphI 1889 GCATGC GCATG^C
2153 GCATGC
StuI 821 AGGCCT AGG^CCT
1257 AGGCCT
Van91I 696 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2339 CCATCCGCTGG
XcmI 1319 CCAAGGTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2795 CCATCGAGAAGGTGG
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AspI AvaI AvaII BalI BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaAI BsaBI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI Tth111I TthHB8I XhoII