Restriction map for pFN21AE3662
 (Circular) (Six-base) MAPSORT of: pFN21AE3662.seq  Check: 9582  from: 1  to: 7633



pFN21AE3662  7633 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3107    3107 
   Size:       7633
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    5443     278 
   Size:         53      83     186     936    3907    2468

  Fragments arranged by size: 

                3907    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2592    3087    4766    4976    1937 
   Size:        655     495    1679     210    4594

  Fragments arranged by size: 

                4594    1679     655     495     210
 
Acc65I G'GTAC_C

Cuts at:   4954    4954 
   Size:       7633
 
AclI AA'CG_TT

Cuts at:   1563    5760    6133    1563 
   Size:       4197     373    3063

  Fragments arranged by size: 

                4197    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3770    3779    3920    5696    7080    7348    1632 
   Size:       2138       9     141    1776    1384     268    1917

  Fragments arranged by size: 

                2138    1917    1776    1384     268     141       9
 
AfeI AGC'GCT

Cuts at:   2507    2507 
   Size:       7633
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    7436
 
AflIII A'CryG_T

Cuts at:   1203    2199    4401    6532    7509    1203 
   Size:        996    2202    2131     977    1327

  Fragments arranged by size: 

                2202    2131    1327     996     977
 
AgeI A'CCGG_T

Cuts at:   6464    6464 
   Size:       7633
 
AhdI GACnn_n'nnGTC

Cuts at:   2965    6362    2965 
   Size:       3397    4236
 
AleI CACnn'nnGTG

Cuts at:   1166    2693    3014    3191    1166 
   Size:       1527     321     177    5608

  Fragments arranged by size: 

                5608    1527     321     177
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3581    4593    1184 
   Size:       2397    1012    4224

  Fragments arranged by size: 

                4224    2397    1012
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4118    4150    4118 
   Size:         32    7601
 
AlwNI CAG_nnn'CTG

Cuts at:   2346    2626    2785    3532    3583    5376    6948    2346 
   Size:        280     159     747      51    1793    1572    3031

  Fragments arranged by size: 

                3031    1793    1572     747     280     159      51
 
ApaI G_GGCC'C

Cuts at:   4102    4102 
   Size:       7633
 
ApaLI G'TGCA_C

Cuts at:   3566    4211    5690    6846    3566 
   Size:        645    1479    1156    4353

  Fragments arranged by size: 

                4353    1479    1156     645
 
ApoI r'AATT_y

Cuts at:   1493    3572    4942    5168    5320    7429    1493 
   Size:       2079    1370     226     152    2109    1697

  Fragments arranged by size: 

                2109    2079    1697    1370     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    6187     160 
   Size:        872    5155    1606

  Fragments arranged by size: 

                5155    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7633
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2609    3116    3910    4307    4958    1870 
   Size:         73     666     507     794     397     651    4545

  Fragments arranged by size: 

                4545     794     666     651     507     397      73
 
BamHI G'GATC_C

Cuts at:   4923    4963    5330    4923 
   Size:         40     367    7226

  Fragments arranged by size: 

                7226     367      40
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2336    2396    2561    2687    2710 
   Size:        325     217     627     549      60     165     126      23

Cuts at:   2710    2767    2912    2950    3026    3925    4714    4954    6409 
   Size:         57     145      38      76     899     789     240    1455

Cuts at:   6409     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     899     789     627     549     325     240
                 217     165     145     126      76      60      57      38
                  23
 
BanII G_rGCy'C

Cuts at:   1391    2013    2127    2298    2733    2870    3216    3321    4102 
   Size:        622     114     171     435     137     346     105     781

Cuts at:   4102    4952    1391 
   Size:        850    4072

  Fragments arranged by size: 

                4072     850     781     622     435     346     171     137
                 114     105
 
BbeI G_GCGC'C

Cuts at:   2340    2340 
   Size:       7633
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3767    3908     928 
   Size:        971    1868     141    4653

  Fragments arranged by size: 

                4653    1868     971     141
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2452    2486    4222    4256    5824    5858    1903 
   Size:         34     515      34    1736      34    1568      34    3678

  Fragments arranged by size: 

                3678    1736    1568     515      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   5525    6735    5525 
   Size:       1210    6423
 
BclI T'GATC_A

Cuts at:   1548    4071    1548 
   Size:       2523    5110
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2072    2570    2681 
   Size:        107     122      71     631     605     400     498     111

Cuts at:   2681    2776    2796    2825    4041    4364    6244     136 
   Size:         95      20      29    1216     323    1880    1525

  Fragments arranged by size: 

                1880    1525    1216     631     605     498     400     323
                 122     111     107      95      71      29      20
 
BglII A'GATC_T

Cuts at:   7628    7628 
   Size:       7633
 
BlpI GC'TnA_GC

Cuts at:   2277    3359    5050    2277 
   Size:       1082    1691    4860

  Fragments arranged by size: 

                4860    1691    1082
 
Bme1580I G_kGCm'C

Cuts at:   1790    2609    2772    2885    2953    3570    3706    4102    4215 
   Size:        819     163     113      68     617     136     396     113

Cuts at:   4215    4582    4636    4719    5694    6850    1790 
   Size:        367      54      83     975    1156    2573

  Fragments arranged by size: 

                2573    1156     975     819     617     396     367     163
                 136     113     113      83      68      54
 
BmgBI CAC'GTC

Cuts at:   1318    4402    1318 
   Size:       3084    4549
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2266    4468    5364 
   Size:        416      53      83     399      24     849    2202     896

Cuts at:   5364    6322     442 
   Size:        958    1753

  Fragments arranged by size: 

                2202    1753     958     896     849     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7633
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2141    2524    3490    2141 
   Size:        383     966    6284

  Fragments arranged by size: 

                6284     966     383
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1997    2136    3230    3356    3391    4316    4763    6293 
   Size:        758     139    1094     126      35     925     447    1530

Cuts at:   6293    1239 
   Size:       2579

  Fragments arranged by size: 

                2579    1530    1094     925     758     447     139     126
                  35
 
Bpu10I CC'TnA_GC

Cuts at:   2886    2886 
   Size:       7633
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2282    4631    4883    5758    6623    6921    2282 
   Size:       2349     252     875     865     298    2994

  Fragments arranged by size: 

                2994    2349     875     865     298     252
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2636    6296     882 
   Size:        617    1137    3660    2219

  Fragments arranged by size: 

                3660    2219    1137     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2312    2485    3769    5424     493 
   Size:        711    1108     173    1284    1655    2702

  Fragments arranged by size: 

                2702    1655    1284    1108     711     173
 
BsaBI GATnn'nnATC

Cuts at:   4073    4343    4928    7627    4073 
   Size:        270     585    2699    4079

  Fragments arranged by size: 

                4079    2699     585     270
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2337    3050    3255    4044 
   Size:         53      83     186     936     804     713     205     789

Cuts at:   4044    5440    5822    6457     275 
   Size:       1396     382     635    1451

  Fragments arranged by size: 

                1451    1396     936     804     789     713     635     382
                 205     186      83      53
 
BsaWI w'CCGG_w

Cuts at:   4377    6066    6464    6738    6885    7296    4377 
   Size:       1689     398     274     147     411    4714

  Fragments arranged by size: 

                4714    1689     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2189    2219    4517    4547    1178 
   Size:         30     165      30     786      30    2298      30    4264

  Fragments arranged by size: 

                4264    2298     786     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2283    2516    3159    1186 
   Size:       1097     233     643    5660

  Fragments arranged by size: 

                5660    1097     643     233
 
BseYI C'CCAG_C

Cuts at:   2962    4691    4891    6836    7332    2962 
   Size:       1729     200    1945     496    3263

  Fragments arranged by size: 

                3263    1945    1729     496     200
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2766    3545    4835    1163 
   Size:        573    1030     779    1290    3961

  Fragments arranged by size: 

                3961    1290    1030     779     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2067    4788    5083    5844    6872    1540 
   Size:        275     186      66    2721     295     761    1028    2301

  Fragments arranged by size: 

                2721    2301    1028     761     295     275     186      66
            
 
BsiHKAI G_wGCw'C

Cuts at:   2013    2127    2298    2892    3570    4215    4776    5694    5779 
   Size:        114     171     594     678     645     561     918      85

Cuts at:   5779    6850    2013 
   Size:       1071    2796

  Fragments arranged by size: 

                2796    1071     918     678     645     594     561     171
                 114      85
 
BsmI GAATG_Cn'

Cuts at:   3134    5144    5237    3134 
   Size:       2010      93    5530

  Fragments arranged by size: 

                5530    2010      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3262    3370    3262 
   Size:        108    7525
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2013    2127    2298    2609    2733    2772    2870 
   Size:        399     223     114     171     311     124      39      98

Cuts at:   2870    2885    2892    2953    3216    3321    3570    3706    4102 
   Size:         15       7      61     263     105     249     136     396

Cuts at:   4102    4215    4582    4636    4719    4776    4952    5694    5779 
   Size:        113     367      54      83      57     176     742      85

Cuts at:   5779    6850    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     396     367     311     263
                 249     223     176     171     136     124     114     113
                 105      98      85      83      61      57      54      39
                  15       7
 
BspEI T'CCGG_A

Cuts at:   7296    7296 
   Size:       7633
 
BspHI T'CATG_A

Cuts at:   2178    4551    5522    7252    2178 
   Size:       2373     971    1730    2559

  Fragments arranged by size: 

                2559    2373    1730     971
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3107    4988     844 
   Size:       2263    1881    3489

  Fragments arranged by size: 

                3489    2263    1881
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2521    4450    4701    5520    1394 
   Size:        429     698    1929     251     819    3507

  Fragments arranged by size: 

                3507    1929     819     698     429     251
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2436    3256    6128    6302      65 
   Size:       1183    1188     820    2872     174    1396

  Fragments arranged by size: 

                2872    1396    1188    1183     820     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2651    2684    3554    3928    3953    3995    4787 
   Size:        110     841      33     870     374      25      42     792

Cuts at:   4787    6277    6464    1700 
   Size:       1490     187    2869

  Fragments arranged by size: 

                2869    1490     870     841     792     374     187     110
                  42      33      25
 
BsrGI T'GTAC_A

Cuts at:     96    3885      96 
   Size:       3789    3844
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7633
 
BssSI C'ACGA_G

Cuts at:   4475    4696    5693    6705    4475 
   Size:        221     997    1012    5403

  Fragments arranged by size: 

                5403    1012     997     221
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2896     794 
   Size:       2102    5531
 
BstBI TT'CG_AA

Cuts at:   6437    6437 
   Size:       7633
 
BstEII G'GTnAC_C

Cuts at:   1184    3268    1184 
   Size:       2084    5549
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3514    4522    3514 
   Size:       1008    6625
 
BstYI r'GATC_y

Cuts at:   1972    3596    3825    4338    4923    4963    5330    5715    5732 
   Size:       1624     229     513     585      40     367     385      17

Cuts at:   5732    7184    7270    7282    7628    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1624    1452     585     513     385     367     346
                 229      86      40      17      12
 
BstZ17I GTA'TAC

Cuts at:   4767    4767 
   Size:       7633
 
Bsu36I CC'TnA_GG

Cuts at:   2907    2955    3057    2907 
   Size:         48     102    7483

  Fragments arranged by size: 

                7483     102      48
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2061    2067    2511    2706    3015    3237 
   Size:        552     939      57       6     444     195     309     222

Cuts at:   3237    4521     513 
   Size:       1284    3625

  Fragments arranged by size: 

                3625    1284     939     552     444     309     222     195
                  57       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2632    4156    4861     487 
   Size:        683      56    1406    1524     705    3259

  Fragments arranged by size: 

                3259    1524    1406     705     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2754    2786    4565    4794    5149    5943    5963    2754 
   Size:         32    1779     229     355     794      20    4424

  Fragments arranged by size: 

                4424    1779     794     355     229      32      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7633
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7598
 
DraI TTT'AAA

Cuts at:   4937    4937 
   Size:       7633
 
DraIII CAC_nnn'GTG

Cuts at:   4480    4480 
   Size:       7633
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6640     809 
   Size:        507    5324    1802

  Fragments arranged by size: 

                5324    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2058    2064    2673 
   Size:         54    1425      50     275      18     228       6     609

Cuts at:   2673    2682    3933    4194    4785    4861    5080    5969    7598 
   Size:          9    1251     261     591      76     219     889    1629

Cuts at:   7598       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1251     889     609     591     275     261
                 228     219      76      54      50      43      18       9
                   6
 
EagI C'GGCC_G

Cuts at:   1537    1812    2064    4785    5080    1537 
   Size:        275     252    2721     295    4090

  Fragments arranged by size: 

                4090    2721     295     275     252
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2721    5563    1353 
   Size:       1368    2842    3423

  Fragments arranged by size: 

                3423    2842    1368
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2983    4022    4525    6220    6594    6740 
   Size:       1064     138    1394    1039     503    1695     374     146

Cuts at:   6740     387 
   Size:       1280

  Fragments arranged by size: 

                1695    1394    1280    1064    1039     503     374     146
                 138
 
EcoICRI GAG'CTC

Cuts at:   2011    2125    2296    2011 
   Size:        114     171    7348

  Fragments arranged by size: 

                7348     171     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    1997    2136    3230    3356    3391    3770    3779 
   Size:        393     365     139    1094     126      35     379       9

Cuts at:   3779    3920    4316    4763    5696    6293    7080    7348    1239 
   Size:        141     396     447     933     597     787     268    1524

  Fragments arranged by size: 

                1524    1094     933     787     597     447     396     393
                 379     365     268     141     139     126      35       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2789    3518    3989    2789 
   Size:        729     471    6433

  Fragments arranged by size: 

                6433     729     471
 
EcoO109I rG'GnC_Cy

Cuts at:   2161    2623    2959    3790    4098    4099    2161 
   Size:        462     336     831     308       1    5695

  Fragments arranged by size: 

                5695     831     462     336     308       1
 
EcoRI G'AATT_C

Cuts at:   3572    4942    5320    3572 
   Size:       1370     378    5885

  Fragments arranged by size: 

                5885    1370     378
 
EcoRV GAT'ATC

Cuts at:   2557    2557 
   Size:       7633
 
FspI TGC'GCA

Cuts at:   6139    6139 
   Size:       7633
 
HaeII r_GCGC'y

Cuts at:   1678    2340    2416    2509    2576    6780    1678 
   Size:        662      76      93      67    4204    2531

  Fragments arranged by size: 

                4204    2531     662      93      76      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2951    2983    3040    3072    4517 
   Size:         32     135      32    1379      32      57      32    1445

Cuts at:   4517    4549    6274    6306    6348    6380    1373 
   Size:         32    1725      32      42      32    2626

  Fragments arranged by size: 

                2626    1725    1445    1379     135      57      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4246    4977    5223     669 
   Size:        988     281    2308     731     246    3079

  Fragments arranged by size: 

                3079    2308     988     731     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       7633
 
HpaI GTT'AAC

Cuts at:   5223    5223 
   Size:       7633
 
KasI G'GCGC_C

Cuts at:   2336    2336 
   Size:       7633
 
KpnI G_GTAC'C

Cuts at:   4958    4958 
   Size:       7633
 
MfeI C'AATT_G

Cuts at:   5232    5232 
   Size:       7633
 
MluI A'CGCG_T

Cuts at:   7509    7509 
   Size:       7633
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2361    3465    4494    6747    6931    1305 
   Size:       1056    1104    1029    2253     184    2007

  Fragments arranged by size: 

                2253    2007    1104    1056    1029     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2675    3935    4196      10 
   Size:         54    1425     343     843    1260     261    3447

  Fragments arranged by size: 

                3447    1425    1260     843     343     261      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2145    2693    3014    3191    3783    4421    4520 
   Size:        648     979     548     321     177     592     638      99

Cuts at:   4520    4754    5591    5950    6109     518 
   Size:        234     837     359     159    2042

  Fragments arranged by size: 

                2042     979     837     648     638     592     548     359
                 321     234     177     159      99
 
MspA1I CmG'CkG

Cuts at:   1606    2063    2069    2114    3066    4150    4178    4350    5049 
   Size:        457       6      45     952    1084      28     172     699

Cuts at:   5049    5726    6874    7119    1606 
   Size:        677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1084     952     699     677     457     245
                 172      45      28       6
 
NaeI GCC'GGC

Cuts at:   2653    2686    3556    3955    3997    4789    2653 
   Size:         33     870     399      42     792    5497

  Fragments arranged by size: 

                5497     870     792     399      42      33
 
NarI GG'CG_CC

Cuts at:   2337    2337 
   Size:       7633
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    4521     513 
   Size:        552     939    2517    3625

  Fragments arranged by size: 

                3625    2517     939     552
 
NdeI CA'TA_TG

Cuts at:    387    3784     387 
   Size:       3397    4236
 
NgoMIV G'CCGG_C

Cuts at:   2651    2684    3554    3953    3995    4787    2651 
   Size:         33     870     399      42     792    5497

  Fragments arranged by size: 

                5497     870     792     399      42      33
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7633
 
NotI GC'GGCC_GC

Cuts at:   2064    5080    2064 
   Size:       3016    4617
 
NspI r_CATG'y

Cuts at:   1132    1229    2191    2203    3898    4162    4991    6536    1132 
   Size:         97     962      12    1695     264     829    1545    2229

  Fragments arranged by size: 

                2229    1695    1545     962     829     264      97      12
            
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7633
 
PciI A'CATG_T

Cuts at:   2199    6532    2199 
   Size:       4333    3300
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2707    4350    1108 
   Size:       1599    1643    4391

  Fragments arranged by size: 

                4391    1643    1599
 
PfoI T'CCnGG_A

Cuts at:   2550    3368    4614    2550 
   Size:        818    1246    5569

  Fragments arranged by size: 

                5569    1246     818
 
PmeI GTTT'AAAC

Cuts at:   4937    4937 
   Size:       7633
 
PmlI CAC'GTG

Cuts at:   2485    2485 
   Size:       7633
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5702    5734    7215    7247    5702 
   Size:         32    1481      32    6088

  Fragments arranged by size: 

                6088    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2161    2959    3790    2161 
   Size:        798     831    6004

  Fragments arranged by size: 

                6004     831     798
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7633
 
PsiI TTA'TAA

Cuts at:   5203    5203 
   Size:       7633
 
PspOMI G'GGCC_C

Cuts at:   4098    4098 
   Size:       7633
 
PstI C_TGCA'G

Cuts at:    830    2113    2622    4149    4985     830 
   Size:       1283     509    1527     836    3478

  Fragments arranged by size: 

                3478    1527    1283     836     509
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7633
 
PvuII CAG'CTG

Cuts at:   2114    4150    4178    2114 
   Size:       2036      28    5569

  Fragments arranged by size: 

                5569    2036      28
 
SacI G_AGCT'C

Cuts at:   2013    2127    2298    2013 
   Size:        114     171    7348

  Fragments arranged by size: 

                7348     171     114
 
SacII CC_GC'GG

Cuts at:   2064    2070    2064 
   Size:          6    7627
 
SalI G'TCGA_C

Cuts at:   1936    4975    1936 
   Size:       3039    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2721    2721 
   Size:       7633
 
SbfI CC_TGCA'GG

Cuts at:   4985    4985 
   Size:       7633
 
ScaI AGT'ACT

Cuts at:   5881    7593    5881 
   Size:       1712    5921
 
SexAI A'CCwGG_T

Cuts at:   3513    4254    4905    3513 
   Size:        741     651    6241

  Fragments arranged by size: 

                6241     741     651
 
SfcI C'TryA_G

Cuts at:    826    1045    2109    2618    3088    4145    4882    4981    6116 
   Size:        219    1064     509     470    1057     737      99    1135

Cuts at:   6116    6797    6988     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1135    1064    1057     737     681     509     470
                 219     191      99
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2681    2681 
   Size:       7633
 
SfoI GGC'GCC

Cuts at:   2338    2338 
   Size:       7633
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7633
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7633
 
SmaI CCC'GGG

Cuts at:   1872    4960    1872 
   Size:       3088    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2297    4646    4898    5737    6638    6900 
   Size:        197     926     354    2349     252     839     901     262

Cuts at:   6900     820 
   Size:       1553

  Fragments arranged by size: 

                2349    1553     926     901     839     354     262     252
                 197
 
SnaBI TAC'GTA

Cuts at:    493    5424     493 
   Size:       4931    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7633
 
SphI G_CATG'C

Cuts at:   1132    3898    4162    4991    1132 
   Size:       2766     264     829    3774

  Fragments arranged by size: 

                3774    2766     829     264
 
SspI AAT'ATT

Cuts at:      5      52    5557       5 
   Size:         47    5505    2081

  Fragments arranged by size: 

                5505    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2828    3264    1517 
   Size:       1311     436    5886

  Fragments arranged by size: 

                5886    1311     436
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3030    3324    4521    5072     513 
   Size:        552     939    1026     294    1197     551    3074

  Fragments arranged by size: 

                3074    1197    1026     939     552     551     294
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2248    3014    4534    5660    5677    5830    6015 
   Size:       1255     668     766    1520    1126      17     153     185

Cuts at:   6015    6460     325 
   Size:        445    1498

  Fragments arranged by size: 

                1520    1498    1255    1126     766     668     445     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2202    3885    4083 
   Size:        274      80      33      51    1443     225    1683     198

Cuts at:   4083    5879    7591      96 
   Size:       1796    1712     138

  Fragments arranged by size: 

                1796    1712    1683    1443     274     225     198     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2744    2776    7593    7625    2744 
   Size:         32    4817      32    2752

  Fragments arranged by size: 

                4817    2752      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    3083    3583    4604    1316 
   Size:       1767     500    1021    4345

  Fragments arranged by size: 

                4345    1767    1021     500
 
XbaI T'CTAG_A

Cuts at:   4969    4969 
   Size:       7633
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3331    4807    3331 
   Size:       1476    6157
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7633
 
XmaI C'CCGG_G

Cuts at:   1870    4958    1870 
   Size:       3088    4545
 
XmnI GAAnn'nnTTC

Cuts at:   5762    5762 
   Size:       7633
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    5441     276 
   Size:         53      83     186     936    3907    2468

  Fragments arranged by size: 

                3907    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII     BaeI    BbvCI    BfrBI    BsiWI     FalI     FseI
    FspAI     NruI     NsiI     PacI    PspXI     PsrI    RsrII    SanDI
     SrfI     SwaI
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