(Circular) (Six-base) MAPSORT of: pFN21AE3662.seq Check: 9582 from: 1 to: 7633 pFN21AE3662 7633 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3107 3107 Size: 7633 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5443 278 Size: 53 83 186 936 3907 2468 Fragments arranged by size: 3907 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 3087 4766 4976 1937 Size: 655 495 1679 210 4594 Fragments arranged by size: 4594 1679 655 495 210 Acc65I G'GTAC_C Cuts at: 4954 4954 Size: 7633 AclI AA'CG_TT Cuts at: 1563 5760 6133 1563 Size: 4197 373 3063 Fragments arranged by size: 4197 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 3779 3920 5696 7080 7348 1632 Size: 2138 9 141 1776 1384 268 1917 Fragments arranged by size: 2138 1917 1776 1384 268 141 9 AfeI AGC'GCT Cuts at: 2507 2507 Size: 7633 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7436 AflIII A'CryG_T Cuts at: 1203 2199 4401 6532 7509 1203 Size: 996 2202 2131 977 1327 Fragments arranged by size: 2202 2131 1327 996 977 AgeI A'CCGG_T Cuts at: 6464 6464 Size: 7633 AhdI GACnn_n'nnGTC Cuts at: 2965 6362 2965 Size: 3397 4236 AleI CACnn'nnGTG Cuts at: 1166 2693 3014 3191 1166 Size: 1527 321 177 5608 Fragments arranged by size: 5608 1527 321 177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3581 4593 1184 Size: 2397 1012 4224 Fragments arranged by size: 4224 2397 1012 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4118 4150 4118 Size: 32 7601 AlwNI CAG_nnn'CTG Cuts at: 2346 2626 2785 3532 3583 5376 6948 2346 Size: 280 159 747 51 1793 1572 3031 Fragments arranged by size: 3031 1793 1572 747 280 159 51 ApaI G_GGCC'C Cuts at: 4102 4102 Size: 7633 ApaLI G'TGCA_C Cuts at: 3566 4211 5690 6846 3566 Size: 645 1479 1156 4353 Fragments arranged by size: 4353 1479 1156 645 ApoI r'AATT_y Cuts at: 1493 3572 4942 5168 5320 7429 1493 Size: 2079 1370 226 152 2109 1697 Fragments arranged by size: 2109 2079 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 6187 160 Size: 872 5155 1606 Fragments arranged by size: 5155 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7633 AvaI C'yCGr_G Cuts at: 1870 1943 2609 3116 3910 4307 4958 1870 Size: 73 666 507 794 397 651 4545 Fragments arranged by size: 4545 794 666 651 507 397 73 BamHI G'GATC_C Cuts at: 4923 4963 5330 4923 Size: 40 367 7226 Fragments arranged by size: 7226 367 40 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2396 2561 2687 2710 Size: 325 217 627 549 60 165 126 23 Cuts at: 2710 2767 2912 2950 3026 3925 4714 4954 6409 Size: 57 145 38 76 899 789 240 1455 Cuts at: 6409 618 Size: 1842 Fragments arranged by size: 1842 1455 899 789 627 549 325 240 217 165 145 126 76 60 57 38 23 BanII G_rGCy'C Cuts at: 1391 2013 2127 2298 2733 2870 3216 3321 4102 Size: 622 114 171 435 137 346 105 781 Cuts at: 4102 4952 1391 Size: 850 4072 Fragments arranged by size: 4072 850 781 622 435 346 171 137 114 105 BbeI G_GCGC'C Cuts at: 2340 2340 Size: 7633 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3767 3908 928 Size: 971 1868 141 4653 Fragments arranged by size: 4653 1868 971 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2452 2486 4222 4256 5824 5858 1903 Size: 34 515 34 1736 34 1568 34 3678 Fragments arranged by size: 3678 1736 1568 515 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5525 6735 5525 Size: 1210 6423 BclI T'GATC_A Cuts at: 1548 4071 1548 Size: 2523 5110 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 2570 2681 Size: 107 122 71 631 605 400 498 111 Cuts at: 2681 2776 2796 2825 4041 4364 6244 136 Size: 95 20 29 1216 323 1880 1525 Fragments arranged by size: 1880 1525 1216 631 605 498 400 323 122 111 107 95 71 29 20 BglII A'GATC_T Cuts at: 7628 7628 Size: 7633 BlpI GC'TnA_GC Cuts at: 2277 3359 5050 2277 Size: 1082 1691 4860 Fragments arranged by size: 4860 1691 1082 Bme1580I G_kGCm'C Cuts at: 1790 2609 2772 2885 2953 3570 3706 4102 4215 Size: 819 163 113 68 617 136 396 113 Cuts at: 4215 4582 4636 4719 5694 6850 1790 Size: 367 54 83 975 1156 2573 Fragments arranged by size: 2573 1156 975 819 617 396 367 163 136 113 113 83 68 54 BmgBI CAC'GTC Cuts at: 1318 4402 1318 Size: 3084 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 4468 5364 Size: 416 53 83 399 24 849 2202 896 Cuts at: 5364 6322 442 Size: 958 1753 Fragments arranged by size: 2202 1753 958 896 849 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7633 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2524 3490 2141 Size: 383 966 6284 Fragments arranged by size: 6284 966 383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1997 2136 3230 3356 3391 4316 4763 6293 Size: 758 139 1094 126 35 925 447 1530 Cuts at: 6293 1239 Size: 2579 Fragments arranged by size: 2579 1530 1094 925 758 447 139 126 35 Bpu10I CC'TnA_GC Cuts at: 2886 2886 Size: 7633 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2282 4631 4883 5758 6623 6921 2282 Size: 2349 252 875 865 298 2994 Fragments arranged by size: 2994 2349 875 865 298 252 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2636 6296 882 Size: 617 1137 3660 2219 Fragments arranged by size: 3660 2219 1137 617 BsaAI yAC'GTr Cuts at: 493 1204 2312 2485 3769 5424 493 Size: 711 1108 173 1284 1655 2702 Fragments arranged by size: 2702 1655 1284 1108 711 173 BsaBI GATnn'nnATC Cuts at: 4073 4343 4928 7627 4073 Size: 270 585 2699 4079 Fragments arranged by size: 4079 2699 585 270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2337 3050 3255 4044 Size: 53 83 186 936 804 713 205 789 Cuts at: 4044 5440 5822 6457 275 Size: 1396 382 635 1451 Fragments arranged by size: 1451 1396 936 804 789 713 635 382 205 186 83 53 BsaWI w'CCGG_w Cuts at: 4377 6066 6464 6738 6885 7296 4377 Size: 1689 398 274 147 411 4714 Fragments arranged by size: 4714 1689 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 4517 4547 1178 Size: 30 165 30 786 30 2298 30 4264 Fragments arranged by size: 4264 2298 786 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2283 2516 3159 1186 Size: 1097 233 643 5660 Fragments arranged by size: 5660 1097 643 233 BseYI C'CCAG_C Cuts at: 2962 4691 4891 6836 7332 2962 Size: 1729 200 1945 496 3263 Fragments arranged by size: 3263 1945 1729 496 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2766 3545 4835 1163 Size: 573 1030 779 1290 3961 Fragments arranged by size: 3961 1290 1030 779 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 4788 5083 5844 6872 1540 Size: 275 186 66 2721 295 761 1028 2301 Fragments arranged by size: 2721 2301 1028 761 295 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2013 2127 2298 2892 3570 4215 4776 5694 5779 Size: 114 171 594 678 645 561 918 85 Cuts at: 5779 6850 2013 Size: 1071 2796 Fragments arranged by size: 2796 1071 918 678 645 594 561 171 114 85 BsmI GAATG_Cn' Cuts at: 3134 5144 5237 3134 Size: 2010 93 5530 Fragments arranged by size: 5530 2010 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3262 3370 3262 Size: 108 7525 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2127 2298 2609 2733 2772 2870 Size: 399 223 114 171 311 124 39 98 Cuts at: 2870 2885 2892 2953 3216 3321 3570 3706 4102 Size: 15 7 61 263 105 249 136 396 Cuts at: 4102 4215 4582 4636 4719 4776 4952 5694 5779 Size: 113 367 54 83 57 176 742 85 Cuts at: 5779 6850 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 367 311 263 249 223 176 171 136 124 114 113 105 98 85 83 61 57 54 39 15 7 BspEI T'CCGG_A Cuts at: 7296 7296 Size: 7633 BspHI T'CATG_A Cuts at: 2178 4551 5522 7252 2178 Size: 2373 971 1730 2559 Fragments arranged by size: 2559 2373 1730 971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 4988 844 Size: 2263 1881 3489 Fragments arranged by size: 3489 2263 1881 BsrBI CCG'CTC Cuts at: 1394 1823 2521 4450 4701 5520 1394 Size: 429 698 1929 251 819 3507 Fragments arranged by size: 3507 1929 819 698 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 2436 3256 6128 6302 65 Size: 1183 1188 820 2872 174 1396 Fragments arranged by size: 2872 1396 1188 1183 820 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 2684 3554 3928 3953 3995 4787 Size: 110 841 33 870 374 25 42 792 Cuts at: 4787 6277 6464 1700 Size: 1490 187 2869 Fragments arranged by size: 2869 1490 870 841 792 374 187 110 42 33 25 BsrGI T'GTAC_A Cuts at: 96 3885 96 Size: 3789 3844 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7633 BssSI C'ACGA_G Cuts at: 4475 4696 5693 6705 4475 Size: 221 997 1012 5403 Fragments arranged by size: 5403 1012 997 221 BstAPI GCAn_nnn'nTGC Cuts at: 794 2896 794 Size: 2102 5531 BstBI TT'CG_AA Cuts at: 6437 6437 Size: 7633 BstEII G'GTnAC_C Cuts at: 1184 3268 1184 Size: 2084 5549 BstXI CCAn_nnnn'nTGG Cuts at: 3514 4522 3514 Size: 1008 6625 BstYI r'GATC_y Cuts at: 1972 3596 3825 4338 4923 4963 5330 5715 5732 Size: 1624 229 513 585 40 367 385 17 Cuts at: 5732 7184 7270 7282 7628 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1624 1452 585 513 385 367 346 229 86 40 17 12 BstZ17I GTA'TAC Cuts at: 4767 4767 Size: 7633 Bsu36I CC'TnA_GG Cuts at: 2907 2955 3057 2907 Size: 48 102 7483 Fragments arranged by size: 7483 102 48 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2067 2511 2706 3015 3237 Size: 552 939 57 6 444 195 309 222 Cuts at: 3237 4521 513 Size: 1284 3625 Fragments arranged by size: 3625 1284 939 552 444 309 222 195 57 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2632 4156 4861 487 Size: 683 56 1406 1524 705 3259 Fragments arranged by size: 3259 1524 1406 705 683 56 BtsI GCAGTG_nn' Cuts at: 2754 2786 4565 4794 5149 5943 5963 2754 Size: 32 1779 229 355 794 20 4424 Fragments arranged by size: 4424 1779 794 355 229 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7633 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7598 DraI TTT'AAA Cuts at: 4937 4937 Size: 7633 DraIII CAC_nnn'GTG Cuts at: 4480 4480 Size: 7633 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6640 809 Size: 507 5324 1802 Fragments arranged by size: 5324 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2064 2673 Size: 54 1425 50 275 18 228 6 609 Cuts at: 2673 2682 3933 4194 4785 4861 5080 5969 7598 Size: 9 1251 261 591 76 219 889 1629 Cuts at: 7598 8 Size: 43 Fragments arranged by size: 1629 1425 1251 889 609 591 275 261 228 219 76 54 50 43 18 9 6 EagI C'GGCC_G Cuts at: 1537 1812 2064 4785 5080 1537 Size: 275 252 2721 295 4090 Fragments arranged by size: 4090 2721 295 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2721 5563 1353 Size: 1368 2842 3423 Fragments arranged by size: 3423 2842 1368 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2983 4022 4525 6220 6594 6740 Size: 1064 138 1394 1039 503 1695 374 146 Cuts at: 6740 387 Size: 1280 Fragments arranged by size: 1695 1394 1280 1064 1039 503 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2125 2296 2011 Size: 114 171 7348 Fragments arranged by size: 7348 171 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1997 2136 3230 3356 3391 3770 3779 Size: 393 365 139 1094 126 35 379 9 Cuts at: 3779 3920 4316 4763 5696 6293 7080 7348 1239 Size: 141 396 447 933 597 787 268 1524 Fragments arranged by size: 1524 1094 933 787 597 447 396 393 379 365 268 141 139 126 35 9 EcoNI CCTnn'n_nnAGG Cuts at: 2789 3518 3989 2789 Size: 729 471 6433 Fragments arranged by size: 6433 729 471 EcoO109I rG'GnC_Cy Cuts at: 2161 2623 2959 3790 4098 4099 2161 Size: 462 336 831 308 1 5695 Fragments arranged by size: 5695 831 462 336 308 1 EcoRI G'AATT_C Cuts at: 3572 4942 5320 3572 Size: 1370 378 5885 Fragments arranged by size: 5885 1370 378 EcoRV GAT'ATC Cuts at: 2557 2557 Size: 7633 FspI TGC'GCA Cuts at: 6139 6139 Size: 7633 HaeII r_GCGC'y Cuts at: 1678 2340 2416 2509 2576 6780 1678 Size: 662 76 93 67 4204 2531 Fragments arranged by size: 4204 2531 662 93 76 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2951 2983 3040 3072 4517 Size: 32 135 32 1379 32 57 32 1445 Cuts at: 4517 4549 6274 6306 6348 6380 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 1445 1379 135 57 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4246 4977 5223 669 Size: 988 281 2308 731 246 3079 Fragments arranged by size: 3079 2308 988 731 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7633 HpaI GTT'AAC Cuts at: 5223 5223 Size: 7633 KasI G'GCGC_C Cuts at: 2336 2336 Size: 7633 KpnI G_GTAC'C Cuts at: 4958 4958 Size: 7633 MfeI C'AATT_G Cuts at: 5232 5232 Size: 7633 MluI A'CGCG_T Cuts at: 7509 7509 Size: 7633 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2361 3465 4494 6747 6931 1305 Size: 1056 1104 1029 2253 184 2007 Fragments arranged by size: 2253 2007 1104 1056 1029 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 3935 4196 10 Size: 54 1425 343 843 1260 261 3447 Fragments arranged by size: 3447 1425 1260 843 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2693 3014 3191 3783 4421 4520 Size: 648 979 548 321 177 592 638 99 Cuts at: 4520 4754 5591 5950 6109 518 Size: 234 837 359 159 2042 Fragments arranged by size: 2042 979 837 648 638 592 548 359 321 234 177 159 99 MspA1I CmG'CkG Cuts at: 1606 2063 2069 2114 3066 4150 4178 4350 5049 Size: 457 6 45 952 1084 28 172 699 Cuts at: 5049 5726 6874 7119 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1084 952 699 677 457 245 172 45 28 6 NaeI GCC'GGC Cuts at: 2653 2686 3556 3955 3997 4789 2653 Size: 33 870 399 42 792 5497 Fragments arranged by size: 5497 870 792 399 42 33 NarI GG'CG_CC Cuts at: 2337 2337 Size: 7633 NcoI C'CATG_G Cuts at: 513 1065 2004 4521 513 Size: 552 939 2517 3625 Fragments arranged by size: 3625 2517 939 552 NdeI CA'TA_TG Cuts at: 387 3784 387 Size: 3397 4236 NgoMIV G'CCGG_C Cuts at: 2651 2684 3554 3953 3995 4787 2651 Size: 33 870 399 42 792 5497 Fragments arranged by size: 5497 870 792 399 42 33 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7633 NotI GC'GGCC_GC Cuts at: 2064 5080 2064 Size: 3016 4617 NspI r_CATG'y Cuts at: 1132 1229 2191 2203 3898 4162 4991 6536 1132 Size: 97 962 12 1695 264 829 1545 2229 Fragments arranged by size: 2229 1695 1545 962 829 264 97 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7633 PciI A'CATG_T Cuts at: 2199 6532 2199 Size: 4333 3300 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 4350 1108 Size: 1599 1643 4391 Fragments arranged by size: 4391 1643 1599 PfoI T'CCnGG_A Cuts at: 2550 3368 4614 2550 Size: 818 1246 5569 Fragments arranged by size: 5569 1246 818 PmeI GTTT'AAAC Cuts at: 4937 4937 Size: 7633 PmlI CAC'GTG Cuts at: 2485 2485 Size: 7633 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5702 5734 7215 7247 5702 Size: 32 1481 32 6088 Fragments arranged by size: 6088 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2959 3790 2161 Size: 798 831 6004 Fragments arranged by size: 6004 831 798 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7633 PsiI TTA'TAA Cuts at: 5203 5203 Size: 7633 PspOMI G'GGCC_C Cuts at: 4098 4098 Size: 7633 PstI C_TGCA'G Cuts at: 830 2113 2622 4149 4985 830 Size: 1283 509 1527 836 3478 Fragments arranged by size: 3478 1527 1283 836 509 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7633 PvuII CAG'CTG Cuts at: 2114 4150 4178 2114 Size: 2036 28 5569 Fragments arranged by size: 5569 2036 28 SacI G_AGCT'C Cuts at: 2013 2127 2298 2013 Size: 114 171 7348 Fragments arranged by size: 7348 171 114 SacII CC_GC'GG Cuts at: 2064 2070 2064 Size: 6 7627 SalI G'TCGA_C Cuts at: 1936 4975 1936 Size: 3039 4594 SapI GCTCTTCn'nnn_ Cuts at: 2721 2721 Size: 7633 SbfI CC_TGCA'GG Cuts at: 4985 4985 Size: 7633 ScaI AGT'ACT Cuts at: 5881 7593 5881 Size: 1712 5921 SexAI A'CCwGG_T Cuts at: 3513 4254 4905 3513 Size: 741 651 6241 Fragments arranged by size: 6241 741 651 SfcI C'TryA_G Cuts at: 826 1045 2109 2618 3088 4145 4882 4981 6116 Size: 219 1064 509 470 1057 737 99 1135 Cuts at: 6116 6797 6988 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1064 1057 737 681 509 470 219 191 99 SfiI GGCCn_nnn'nGGCC Cuts at: 2681 2681 Size: 7633 SfoI GGC'GCC Cuts at: 2338 2338 Size: 7633 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7633 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7633 SmaI CCC'GGG Cuts at: 1872 4960 1872 Size: 3088 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 4646 4898 5737 6638 6900 Size: 197 926 354 2349 252 839 901 262 Cuts at: 6900 820 Size: 1553 Fragments arranged by size: 2349 1553 926 901 839 354 262 252 197 SnaBI TAC'GTA Cuts at: 493 5424 493 Size: 4931 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7633 SphI G_CATG'C Cuts at: 1132 3898 4162 4991 1132 Size: 2766 264 829 3774 Fragments arranged by size: 3774 2766 829 264 SspI AAT'ATT Cuts at: 5 52 5557 5 Size: 47 5505 2081 Fragments arranged by size: 5505 2081 47 StuI AGG'CCT Cuts at: 1517 2828 3264 1517 Size: 1311 436 5886 Fragments arranged by size: 5886 1311 436 StyI C'CwwG_G Cuts at: 513 1065 2004 3030 3324 4521 5072 513 Size: 552 939 1026 294 1197 551 3074 Fragments arranged by size: 3074 1197 1026 939 552 551 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2248 3014 4534 5660 5677 5830 6015 Size: 1255 668 766 1520 1126 17 153 185 Cuts at: 6015 6460 325 Size: 445 1498 Fragments arranged by size: 1520 1498 1255 1126 766 668 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 3885 4083 Size: 274 80 33 51 1443 225 1683 198 Cuts at: 4083 5879 7591 96 Size: 1796 1712 138 Fragments arranged by size: 1796 1712 1683 1443 274 225 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 7593 7625 2744 Size: 32 4817 32 2752 Fragments arranged by size: 4817 2752 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3083 3583 4604 1316 Size: 1767 500 1021 4345 Fragments arranged by size: 4345 1767 1021 500 XbaI T'CTAG_A Cuts at: 4969 4969 Size: 7633 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3331 4807 3331 Size: 1476 6157 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7633 XmaI C'CCGG_G Cuts at: 1870 4958 1870 Size: 3088 4545 XmnI GAAnn'nnTTC Cuts at: 5762 5762 Size: 7633 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5441 276 Size: 53 83 186 936 3907 2468 Fragments arranged by size: 3907 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SrfI SwaI