Restriction map for
pFN21AE3662
(Linear) (Six-base) MAPSORT of: KIEE3662.seq Check: 982 from: 1 to: 2928
KIEE3662.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1102 2928
Size: 1102 1826
AccI GT'mk_AC
Cuts at: 0 587 1082 2761 2928
Size: 587 495 1679 167
Fragments arranged by size:
1679 587 495 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1765 1774 1915 2928
Size: 1765 9 141 1013
Fragments arranged by size:
1765 1013 141 9
AfeI AGC'GCT
Cuts at: 0 502 2928
Size: 502 2426
AflIII A'CryG_T
Cuts at: 0 194 2396 2928
Size: 194 2202 532
Fragments arranged by size:
2202 532 194
AhdI GACnn_n'nnGTC
Cuts at: 0 960 2928
Size: 960 1968
AleI CACnn'nnGTG
Cuts at: 0 688 1009 1186 2928
Size: 688 321 177 1742
Fragments arranged by size:
1742 688 321 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1576 2588 2928
Size: 1576 1012 340
Fragments arranged by size:
1576 1012 340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2113 2145 2928
Size: 2113 32 783
Fragments arranged by size:
2113 783 32
AlwNI CAG_nnn'CTG
Cuts at: 0 341 621 780 1527 1578 2928
Size: 341 280 159 747 51 1350
Fragments arranged by size:
1350 747 341 280 159 51
ApaI G_GGCC'C
Cuts at: 0 2097 2928
Size: 2097 831
ApaLI G'TGCA_C
Cuts at: 0 1561 2206 2928
Size: 1561 645 722
Fragments arranged by size:
1561 722 645
ApoI r'AATT_y
Cuts at: 0 1567 2928
Size: 1567 1361
AvaI C'yCGr_G
Cuts at: 0 604 1111 1905 2302 2928
Size: 604 507 794 397 626
Fragments arranged by size:
794 626 604 507 397
BamHI G'GATC_C
Cuts at: 0 2918 2928
Size: 2918 10
BanI G'GyrC_C
Cuts at: 0 331 391 556 682 705 762 907 945
Size: 331 60 165 126 23 57 145 38
Cuts at: 945 1021 1920 2709 2928
Size: 76 899 789 219
Fragments arranged by size:
899 789 331 219 165 145 126 76
60 57 38 23
BanII G_rGCy'C
Cuts at: 0 8 122 293 728 865 1211 1316 2097
Size: 8 114 171 435 137 346 105 781
Cuts at: 2097 2928
Size: 831
Fragments arranged by size:
831 781 435 346 171 137 114 105
8
BbeI G_GCGC'C
Cuts at: 0 335 2928
Size: 335 2593
BbsI GAAGACnn'nnnn_
Cuts at: 0 1762 1903 2928
Size: 1762 141 1025
Fragments arranged by size:
1762 1025 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 481 2217 2251 2928
Size: 447 34 1736 34 677
Fragments arranged by size:
1736 677 447 34 34
BclI T'GATC_A
Cuts at: 0 2066 2928
Size: 2066 862
BglI GCCn_nnn'nGGC
Cuts at: 0 67 565 676 771 791 820 2036 2359
Size: 67 498 111 95 20 29 1216 323
Cuts at: 2359 2928
Size: 569
Fragments arranged by size:
1216 569 498 323 111 95 67 29
20
BlpI GC'TnA_GC
Cuts at: 0 272 1354 2928
Size: 272 1082 1574
Fragments arranged by size:
1574 1082 272
Bme1580I G_kGCm'C
Cuts at: 0 604 767 880 948 1565 1701 2097 2210
Size: 604 163 113 68 617 136 396 113
Cuts at: 2210 2577 2631 2714 2928
Size: 367 54 83 214
Fragments arranged by size:
617 604 396 367 214 163 136 113
113 83 68 54
BmgBI CAC'GTC
Cuts at: 0 2397 2928
Size: 2397 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 2463 2928
Size: 261 2202 465
Fragments arranged by size:
2202 465 261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 519 1485 2928
Size: 136 383 966 1443
Fragments arranged by size:
1443 966 383 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1225 1351 1386 2311 2758 2928
Size: 131 1094 126 35 925 447 170
Fragments arranged by size:
1094 925 447 170 131 126 35
Bpu10I CC'TnA_GC
Cuts at: 0 881 2928
Size: 881 2047
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 2626 2878 2928
Size: 277 2349 252 50
Fragments arranged by size:
2349 277 252 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 631 2928
Size: 631 2297
BsaAI yAC'GTr
Cuts at: 0 307 480 1764 2928
Size: 307 173 1284 1164
Fragments arranged by size:
1284 1164 307 173
BsaBI GATnn'nnATC
Cuts at: 0 2068 2338 2923 2928
Size: 2068 270 585 5
Fragments arranged by size:
2068 585 270 5
BsaHI Gr'CG_yC
Cuts at: 0 332 1045 1250 2039 2928
Size: 332 713 205 789 889
Fragments arranged by size:
889 789 713 332 205
BsaWI w'CCGG_w
Cuts at: 0 2372 2928
Size: 2372 556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 2512 2542 2928
Size: 184 30 2298 30 386
Fragments arranged by size:
2298 386 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 278 511 1154 2928
Size: 278 233 643 1774
Fragments arranged by size:
1774 643 278 233
BseYI C'CCAG_C
Cuts at: 0 957 2686 2886 2928
Size: 957 1729 200 42
Fragments arranged by size:
1729 957 200 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1540 2830 2928
Size: 761 779 1290 98
Fragments arranged by size:
1290 779 761 98
BsiEI CG_ry'CG
Cuts at: 0 62 2783 2928
Size: 62 2721 145
Fragments arranged by size:
2721 145 62
BsiHKAI G_wGCw'C
Cuts at: 0 8 122 293 887 1565 2210 2771 2928
Size: 8 114 171 594 678 645 561 157
Fragments arranged by size:
678 645 594 561 171 157 114 8
BsmI GAATG_Cn'
Cuts at: 0 1129 2928
Size: 1129 1799
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1257 1365 2928
Size: 1257 108 1563
Fragments arranged by size:
1563 1257 108
Bsp1286I G_dGCh'C
Cuts at: 0 8 122 293 604 728 767 865 880
Size: 8 114 171 311 124 39 98 15
Cuts at: 880 887 948 1211 1316 1565 1701 2097 2210
Size: 7 61 263 105 249 136 396 113
Cuts at: 2210 2577 2631 2714 2771 2928
Size: 367 54 83 57 157
Fragments arranged by size:
396 367 311 263 249 171 157 136
124 114 113 105 98 83 61 57
54 39 15 8 7
BspHI T'CATG_A
Cuts at: 0 173 2546 2928
Size: 173 2373 382
Fragments arranged by size:
2373 382 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1102 2928
Size: 1102 1826
BsrBI CCG'CTC
Cuts at: 0 516 2445 2696 2928
Size: 516 1929 251 232
Fragments arranged by size:
1929 516 251 232
BsrDI GCAATG_nn'
Cuts at: 0 431 1251 2928
Size: 431 820 1677
Fragments arranged by size:
1677 820 431
BsrFI r'CCGG_y
Cuts at: 0 646 679 1549 1923 1948 1990 2782 2928
Size: 646 33 870 374 25 42 792 146
Fragments arranged by size:
870 792 646 374 146 42 33 25
BsrGI T'GTAC_A
Cuts at: 0 1880 2928
Size: 1880 1048
BssSI C'ACGA_G
Cuts at: 0 2470 2691 2928
Size: 2470 221 237
Fragments arranged by size:
2470 237 221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 891 2928
Size: 891 2037
BstEII G'GTnAC_C
Cuts at: 0 1263 2928
Size: 1263 1665
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1509 2517 2928
Size: 1509 1008 411
Fragments arranged by size:
1509 1008 411
BstYI r'GATC_y
Cuts at: 0 1591 1820 2333 2918 2928
Size: 1591 229 513 585 10
Fragments arranged by size:
1591 585 513 229 10
BstZ17I GTA'TAC
Cuts at: 0 2762 2928
Size: 2762 166
Bsu36I CC'TnA_GG
Cuts at: 0 902 950 1052 2928
Size: 902 48 102 1876
Fragments arranged by size:
1876 902 102 48
BtgI C'CryG_G
Cuts at: 0 56 62 506 701 1010 1232 2516 2928
Size: 56 6 444 195 309 222 1284 412
Fragments arranged by size:
1284 444 412 309 222 195 56 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 627 2151 2856 2928
Size: 627 1524 705 72
Fragments arranged by size:
1524 705 627 72
BtsI GCAGTG_nn'
Cuts at: 0 749 781 2560 2789 2928
Size: 749 32 1779 229 139
Fragments arranged by size:
1779 749 229 139 32
DraIII CAC_nnn'GTG
Cuts at: 0 2475 2928
Size: 2475 453
EaeI y'GGCC_r
Cuts at: 0 53 59 668 677 1928 2189 2780 2856
Size: 53 6 609 9 1251 261 591 76
Cuts at: 2856 2928
Size: 72
Fragments arranged by size:
1251 609 591 261 76 72 53 9
6
EagI C'GGCC_G
Cuts at: 0 59 2780 2928
Size: 59 2721 148
Fragments arranged by size:
2721 148 59
EarI CTCTTCn'nnn_
Cuts at: 0 716 2928
Size: 716 2212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 978 2017 2520 2928
Size: 978 1039 503 408
Fragments arranged by size:
1039 978 503 408
EcoICRI GAG'CTC
Cuts at: 0 6 120 291 2928
Size: 6 114 171 2637
Fragments arranged by size:
2637 171 114 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1225 1351 1386 1765 1774 1915 2311
Size: 131 1094 126 35 379 9 141 396
Cuts at: 2311 2758 2928
Size: 447 170
Fragments arranged by size:
1094 447 396 379 170 141 131 126
35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 1513 1984 2928
Size: 784 729 471 944
Fragments arranged by size:
944 784 729 471
EcoO109I rG'GnC_Cy
Cuts at: 0 156 618 954 1785 2093 2094 2928
Size: 156 462 336 831 308 1 834
Fragments arranged by size:
834 831 462 336 308 156 1
EcoRI G'AATT_C
Cuts at: 0 1567 2928
Size: 1567 1361
EcoRV GAT'ATC
Cuts at: 0 552 2928
Size: 552 2376
HaeII r_GCGC'y
Cuts at: 0 335 411 504 571 2928
Size: 335 76 93 67 2357
Fragments arranged by size:
2357 335 93 76 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 946 978 1035 1067 2512 2544 2928
Size: 946 32 57 32 1445 32 384
Fragments arranged by size:
1445 946 384 57 32 32 32
HincII GTy'rAC
Cuts at: 0 2241 2928
Size: 2241 687
KasI G'GCGC_C
Cuts at: 0 331 2928
Size: 331 2597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1460 2489 2928
Size: 356 1104 1029 439
Fragments arranged by size:
1104 1029 439 356
MscI TGG'CCA
Cuts at: 0 670 1930 2191 2928
Size: 670 1260 261 737
Fragments arranged by size:
1260 737 670 261
MslI CAynn'nnrTG
Cuts at: 0 140 688 1009 1186 1778 2416 2515 2749
Size: 140 548 321 177 592 638 99 234
Cuts at: 2749 2928
Size: 179
Fragments arranged by size:
638 592 548 321 234 179 177 140
99
MspA1I CmG'CkG
Cuts at: 0 58 64 109 1061 2145 2173 2345 2928
Size: 58 6 45 952 1084 28 172 583
Fragments arranged by size:
1084 952 583 172 58 45 28 6
NaeI GCC'GGC
Cuts at: 0 648 681 1551 1950 1992 2784 2928
Size: 648 33 870 399 42 792 144
Fragments arranged by size:
870 792 648 399 144 42 33
NarI GG'CG_CC
Cuts at: 0 332 2928
Size: 332 2596
NcoI C'CATG_G
Cuts at: 0 2516 2928
Size: 2516 412
NdeI CA'TA_TG
Cuts at: 0 1779 2928
Size: 1779 1149
NgoMIV G'CCGG_C
Cuts at: 0 646 679 1549 1948 1990 2782 2928
Size: 646 33 870 399 42 792 146
Fragments arranged by size:
870 792 646 399 146 42 33
NotI GC'GGCC_GC
Cuts at: 0 59 2928
Size: 59 2869
NspI r_CATG'y
Cuts at: 0 186 198 1893 2157 2928
Size: 186 12 1695 264 771
Fragments arranged by size:
1695 771 264 186 12
PciI A'CATG_T
Cuts at: 0 194 2928
Size: 194 2734
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 2345 2928
Size: 702 1643 583
Fragments arranged by size:
1643 702 583
PfoI T'CCnGG_A
Cuts at: 0 545 1363 2609 2928
Size: 545 818 1246 319
Fragments arranged by size:
1246 818 545 319
PmlI CAC'GTG
Cuts at: 0 480 2928
Size: 480 2448
PpuMI rG'GwC_Cy
Cuts at: 0 156 954 1785 2928
Size: 156 798 831 1143
Fragments arranged by size:
1143 831 798 156
PspOMI G'GGCC_C
Cuts at: 0 2093 2928
Size: 2093 835
PstI C_TGCA'G
Cuts at: 0 108 617 2144 2928
Size: 108 509 1527 784
Fragments arranged by size:
1527 784 509 108
PvuII CAG'CTG
Cuts at: 0 109 2145 2173 2928
Size: 109 2036 28 755
Fragments arranged by size:
2036 755 109 28
SacI G_AGCT'C
Cuts at: 0 8 122 293 2928
Size: 8 114 171 2635
Fragments arranged by size:
2635 171 114 8
SacII CC_GC'GG
Cuts at: 0 59 65 2928
Size: 59 6 2863
Fragments arranged by size:
2863 59 6
SapI GCTCTTCn'nnn_
Cuts at: 0 716 2928
Size: 716 2212
SexAI A'CCwGG_T
Cuts at: 0 1508 2249 2900 2928
Size: 1508 741 651 28
Fragments arranged by size:
1508 741 651 28
SfcI C'TryA_G
Cuts at: 0 104 613 1083 2140 2877 2928
Size: 104 509 470 1057 737 51
Fragments arranged by size:
1057 737 509 470 104 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 676 2928
Size: 676 2252
SfoI GGC'GCC
Cuts at: 0 333 2928
Size: 333 2595
SmlI C'TyrA_G
Cuts at: 0 292 2641 2893 2928
Size: 292 2349 252 35
Fragments arranged by size:
2349 292 252 35
SphI G_CATG'C
Cuts at: 0 1893 2157 2928
Size: 1893 264 771
Fragments arranged by size:
1893 771 264
StuI AGG'CCT
Cuts at: 0 823 1259 2928
Size: 823 436 1669
Fragments arranged by size:
1669 823 436
StyI C'CwwG_G
Cuts at: 0 1025 1319 2516 2928
Size: 1025 294 1197 412
Fragments arranged by size:
1197 1025 412 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 243 1009 2529 2928
Size: 243 766 1520 399
Fragments arranged by size:
1520 766 399 243
TatI w'GTAC_w
Cuts at: 0 197 1880 2078 2928
Size: 197 1683 198 850
Fragments arranged by size:
1683 850 198 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 2928
Size: 739 32 2157
Fragments arranged by size:
2157 739 32
Tth111I GACn'n_nGTC
Cuts at: 0 1078 1578 2599 2928
Size: 1078 500 1021 329
Fragments arranged by size:
1078 1021 500 329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1326 2802 2928
Size: 1326 1476 126
Fragments arranged by size:
1476 1326 126
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NotI NspI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII
SapI SexAI SfcI SfiI SfoI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BfrBI BglII BmtI BsiWI
BspEI BssHII BstBI ClaI CspCI DraI DrdI FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NheI NruI NsiI PacI PasI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI