Restriction map for
pFN21AB6581
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaI BsgI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI DdeI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MluNI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI PstI
PvuI PvuII RcaI RsaI SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 961 GACGTC GACGT^C
AccIII 989 TCCGGA T^CCGGA
AclI 692 AACGTT AA^CGTT
AflII 954 CTTAAG C^TTAAG
ApaI 383 GGGCCC GGGCC^C
BamHI 942 GGATCC G^GATCC
BanII 383 GGGCCC G[GA]GC[CT]^C
BbsI 599 GAAGAC GAAGAC(2/6)
BciVI 1089 GGATAC GTATCC(6/5)
BclI 275 TGATCA T^GATCA
BfaI 928 CTAG C^TAG
BfrI 954 CTTAAG C^TTAAG
BglI 385 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 383 GGGCCC G[AGT]GC[ACT]^C
BpuAI 599 GAAGAC GAAGAC(2/6)
BsaBI 201 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 989 TCCGGA [AT]^CCGG[AT]
BseAI 989 TCCGGA T^CCGGA
BsmBI 959 GAGACG CGTCTC(1/5)
BsmFI 1004 GGGAC GGGAC(10/14)
BsmI 477 GAATGC GAATGC(1/-1)
Bsp1286I 383 GGGCCC G[AGT]GC[ACT]^C
BspEI 989 TCCGGA T^CCGGA
BsrBI 174 GAGCGG CCGCTC(-3/-3)
BsrDI 845 GCAATG GCAATG(2/0)
BsrFI 488 ACCGGC [GA]^CCGG[CT]
BsrI 507 ACTGG ACTGG(1/-1)
BstAPI 1116 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 345 CCAACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 488 ACCGGC [GA]^CCGG[CT]
CpoI 22 CGGTCCG CG^G[AT]CCG
DraII 383 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 383 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 166 GCGGG CCCGC(4/6)
FbaI 275 TGATCA T^GATCA
HgaI 492 GCGTC GACGC(5/10)
HindIII 313 AAGCTT A^AGCTT
MaeI 928 CTAG C^TAG
MamI 201 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 989 TCCGGA T^CCGGA
MspA1I 465 CAGCGG C[AC]G^C[GT]G
NcoI 389 CCATGG C^CATGG
PaeR7I 305 CTCGAG C^TCGAG
PflMI 399 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 799 GAGTC GAGTC(4/5)
PshAI 487 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 692 AACGTT AA^CGTT
RsrII 22 CGGTCCG CG^G[AT]CCG
SexAI 758 ACCAGGT A^CC[AT]GGT
SmaI 380 CCCGGG CCC^GGG
SspI 524 AATATT AAT^ATT
TfiI 514 GAATC G^A[AT]TC
Van91I 399 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 305 CTCGAG C^TCGAG
XmaCI 380 CCCGGG C^CCGGG
XmaI 380 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 491 GGCGTC G[GA]^CG[CT]C
961 GACGTC
AluI 314 AGCT AG^CT
1118 AGCT
AvaII 23 GGTCC G^G[AT]CC
1005 GGACC
BsaHI 491 GGCGTC G[GA]^CG[CT]C
961 GACGTC
BseRI 244 GAGGAG GAGGAG(10/8)
889 GAGGAG
BstYI 149 AGATCC [GA]^GATC[CT]
942 GGATCC
DsaI 213 CCACGG C^C[GA][CT]GG
389 CCATGG
HaeIII 72 GGCC GG^CC
384 GGCC
Hin1I 491 GGCGTC G[GA]^CG[CT]C
961 GACGTC
HinfI 514 GAATC G^A[ACGT]TC
799 GAGTC
MaeIII 718 GTGAC ^GT[ACGT]AC
1079 GTGAC
MflI 149 AGATCC [GA]^GATC[CT]
942 GGATCC
MseI 868 TTAA T^TAA
955 TTAA
SfcI 264 CTACAG C^T[GA][CT]AG
1126 CTACAG
Tru9I 868 TTAA T^TAA
955 TTAA
Tsp45I 718 GTGAC ^GT[GC]AC
1079 GTGAC
XhoII 149 AGATCC [GA]^GATC[CT]
942 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcsI AlwI ApoI
AvaI BbvI BcnI BsaJI BsiYI
BslI BsmAI BsoBI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII EcoT14I Fnu4HI
FokI HapII HhaI HinP1I HpaII
HphI ItaI MaeII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TseI Tsp509I TspRI TthHB8I