Restriction map for pFN21AB9957
 (Circular) (Six-base) MAPSORT of: pFN21AB9957.seq  Check: 353  from: 1  to: 6319



pFN21AB9957  6319 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3320    3391    3320 
   Size:         71    6248
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4129     278 
   Size:         53      83     186     936    2593    2468

  Fragments arranged by size: 

                2593    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2959    3169    3662    1937 
   Size:       1022     210     493    4594

  Fragments arranged by size: 

                4594    1022     493     210
 
Acc65I G'GTAC_C

Cuts at:   3262    3640    3262 
   Size:        378    5941
 
AclI AA'CG_TT

Cuts at:   1563    3112    4446    4819    1563 
   Size:       1549    1334     373    3063

  Fragments arranged by size: 

                3063    1549    1334     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2148    2199    2373    2429    3047    4382    5766    6034 
   Size:        516      51     174      56     618    1335    1384     268

Cuts at:   6034    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384    1335     618     516     268     174      56
                  51
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6122
 
AflIII A'CryG_T

Cuts at:   1203    5218    6195    1203 
   Size:       4015     977    1327

  Fragments arranged by size: 

                4015    1327     977
 
AgeI A'CCGG_T

Cuts at:   2106    5150    2106 
   Size:       3044    3275
 
AhdI GACnn_n'nnGTC

Cuts at:   5048    5048 
   Size:       6319
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6319
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3217    1184 
   Size:       2033    4286
 
AlwNI CAG_nnn'CTG

Cuts at:   2035    3191    4062    5634    2035 
   Size:       1156     871    1572    2720

  Fragments arranged by size: 

                2720    1572    1156     871
 
ApaLI G'TGCA_C

Cuts at:   4376    5532    4376 
   Size:       1156    5163
 
ApoI r'AATT_y

Cuts at:   1493    2896    3048    3598    3628    3854    4006    6115    1493 
   Size:       1403     152     550      30     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1403     550     226     152     152      30
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4873     160 
   Size:        872    3841    1606

  Fragments arranged by size: 

                3841    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6319
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3644    1870 
   Size:         73    1701    4545

  Fragments arranged by size: 

                4545    1701      73
 
BamHI G'GATC_C

Cuts at:   3649    4016    3649 
   Size:        367    5952
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3033    3262    3640    5095     618 
   Size:        325     217     627    1246     229     378    1455    1842

  Fragments arranged by size: 

                1842    1455    1246     627     378     325     229     217
            
 
BanII G_rGCy'C

Cuts at:   1391    3367    3583    3638    1391 
   Size:       1976     216      55    4072

  Fragments arranged by size: 

                4072    1976     216      55
 
BbeI G_GCGC'C

Cuts at:   3037    3037 
   Size:       6319
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3377    3385     928 
   Size:        971    1478       8    3862

  Fragments arranged by size: 

                3862    1478     971       8
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4510    4544    1903 
   Size:         34    2573      34    3678

  Fragments arranged by size: 

                3678    2573      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4211    5421    4211 
   Size:       1210    5109
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6319
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4930     136 
   Size:        107     122      71     631     605    3258    1525

  Fragments arranged by size: 

                3258    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2404    2545    6314    2404 
   Size:        141    3769    2409

  Fragments arranged by size: 

                3769    2409     141
 
BlpI GC'TnA_GC

Cuts at:   3736    3736 
   Size:       6319
 
Bme1580I G_kGCm'C

Cuts at:   1790    2446    4380    5536    1790 
   Size:        656    1934    1156    2573

  Fragments arranged by size: 

                2573    1934    1156     656
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6319
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2299    4050    5008 
   Size:        416      53      83     399      24     882    1751     958

Cuts at:   5008     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1751     958     882     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6319
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2711    3447    3597    4979    1239 
   Size:       1472     736     150    1382    2579

  Fragments arranged by size: 

                2579    1472    1382     736     150
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4444    5309    5607    4444 
   Size:        865     298    5156

  Fragments arranged by size: 

                5156     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2541    3017    3410    4982     882 
   Size:        617    1042     476     393    1572    2219

  Fragments arranged by size: 

                2219    1572    1042     617     476     393
 
BsaAI yAC'GTr

Cuts at:    493    1204    4110     493 
   Size:        711    2906    2702

  Fragments arranged by size: 

                2906    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6313    6313 
   Size:       6319
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3034    4126    4508    5143 
   Size:         53      83     186     936    1501    1092     382     635

Cuts at:   5143     275 
   Size:       1451

  Fragments arranged by size: 

                1501    1451    1092     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2106    2448    4752    5150    5424    5571    5982    2106 
   Size:        342    2304     398     274     147     411    2443

  Fragments arranged by size: 

                2443    2304     411     398     342     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6094

  Fragments arranged by size: 

                6094     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6319
 
BseYI C'CCAG_C

Cuts at:   3325    5522    6018    3325 
   Size:       2197     496    3626

  Fragments arranged by size: 

                3626    2197     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5746
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3769    4530    5558    1540 
   Size:        275     186    1768     761    1028    2301

  Fragments arranged by size: 

                2301    1768    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2312    3583    4380    4465    5536    2312 
   Size:       1271     797      85    1071    3095

  Fragments arranged by size: 

                3095    1271    1071     797      85
 
BsmI GAATG_Cn'

Cuts at:   2364    3830    3923    2364 
   Size:       1466      93    4760

  Fragments arranged by size: 

                4760    1466      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2312    2446    3367    3583    3638    4380    4465 
   Size:        399     522     134     921     216      55     742      85

Cuts at:   4465    5536    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     921     742     522     399     216     134
                  85      55
 
BspEI T'CCGG_A

Cuts at:   2448    5982    2448 
   Size:       3534    2785
 
BspHI T'CATG_A

Cuts at:   2632    4208    5938    2632 
   Size:       1576    1730    3013

  Fragments arranged by size: 

                3013    1730    1576
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3320    3391    3674     844 
   Size:       2476      71     283    3489

  Fragments arranged by size: 

                3489    2476     283      71
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4206    1394 
   Size:        429    2383    3507

  Fragments arranged by size: 

                3507    2383     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3204    3525    4814    4988      65 
   Size:       1183    1956     321    1289     174    1396

  Fragments arranged by size: 

                1956    1396    1289    1183     321     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2106    4963    5150    1700 
   Size:        110     296    2857     187    2869

  Fragments arranged by size: 

                2869    2857     296     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6319
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6319
 
BssSI C'ACGA_G

Cuts at:   4379    5391    4379 
   Size:       1012    5307
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3065     794 
   Size:       2271    4048
 
BstBI TT'CG_AA

Cuts at:   3595    5123    3595 
   Size:       1528    4791
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6319
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3360    3360 
   Size:       6319
 
BstYI r'GATC_y

Cuts at:   1972    2404    2545    3321    3649    4016    4401    4418    5870 
   Size:        432     141     776     328     367     385      17    1452

Cuts at:   5870    5956    5968    6314    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     776     432     385     367     346     328
                 141      86      17      12
 
BstZ17I GTA'TAC

Cuts at:   2960    3170    2960 
   Size:        210    6109
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4828

  Fragments arranged by size: 

                4828     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5580

  Fragments arranged by size: 

                5580     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3835    4629    4649    3835 
   Size:        794      20    5505

  Fragments arranged by size: 

                5505     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6319
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6284
 
DraI TTT'AAA

Cuts at:   2341    3623    2341 
   Size:       1282    5037
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5326     809 
   Size:        507    4010    1802

  Fragments arranged by size: 

                4010    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3766    4655    6284 
   Size:         54    1425      50     275      18    1936     889    1629

Cuts at:   6284       8 
   Size:         43

  Fragments arranged by size: 

                1936    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3766    1537 
   Size:        275    1954    4090

  Fragments arranged by size: 

                4090    1954     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3354    4249    1353 
   Size:       2001     895    3423

  Fragments arranged by size: 

                3423    2001     895
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4906    5280    5426     387 
   Size:       1064     138    3317     374     146    1280

  Fragments arranged by size: 

                3317    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3581    3581 
   Size:       6319
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2148    2199    2373    2429    2711    3047    3447 
   Size:        393     516      51     174      56     282     336     400

Cuts at:   3447    3597    4382    4979    5766    6034    1239 
   Size:        150     785     597     787     268    1524

  Fragments arranged by size: 

                1524     787     785     597     516     400     393     336
                 282     268     174     150      56      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2450    2450 
   Size:       6319
 
EcoRI G'AATT_C

Cuts at:   3628    4006    3628 
   Size:        378    5941
 
FspI TGC'GCA

Cuts at:   4825    4825 
   Size:       6319
 
HaeII r_GCGC'y

Cuts at:   1678    3037    5466    1678 
   Size:       1359    2429    2531

  Fragments arranged by size: 

                2531    2429    1359
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3349    3381    3445    3477    4960 
   Size:         32     135      32    1777      32      64      32    1483

Cuts at:   4960    4992    5034    5066    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1777    1483     135      64      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3663    3909     669 
   Size:        988     281    1725     246    3079

  Fragments arranged by size: 

                3079    1725     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2181     748 
   Size:       1433    4886
 
HpaI GTT'AAC

Cuts at:   3909    3909 
   Size:       6319
 
KasI G'GCGC_C

Cuts at:   3033    3033 
   Size:       6319
 
KpnI G_GTAC'C

Cuts at:   3266    3644    3266 
   Size:        378    5941
 
MfeI C'AATT_G

Cuts at:   2592    3918    2592 
   Size:       1326    4993
 
MluI A'CGCG_T

Cuts at:   6195    6195 
   Size:       6319
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2337    3534    5433    5617    1305 
   Size:       1032    1197    1899     184    2007

  Fragments arranged by size: 

                2007    1899    1197    1032     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4497

  Fragments arranged by size: 

                4497    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2090    4277    4636    4795     518 
   Size:        648     924    2187     359     159    2042

  Fragments arranged by size: 

                2187    2042     924     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3735    4412    5560    5805    1606 
   Size:       2129     677    1148     245    2120

  Fragments arranged by size: 

                2129    2120    1148     677     245
 
NarI GG'CG_CC

Cuts at:   3034    3034 
   Size:       6319
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4828

  Fragments arranged by size: 

                4828     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6319
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6319
 
NotI GC'GGCC_GC

Cuts at:   3766    3766 
   Size:       6319
 
NspI r_CATG'y

Cuts at:   1132    1229    2434    3553    3677    5222    1132 
   Size:         97    1205    1119     124    1545    2229

  Fragments arranged by size: 

                2229    1545    1205    1119     124      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6319
 
PciI A'CATG_T

Cuts at:   5218    5218 
   Size:       6319
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3332    1108 
   Size:       2224    4095
 
PmeI GTTT'AAAC

Cuts at:   3623    3623 
   Size:       6319
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4388    4420    5901    5933    4388 
   Size:         32    1481      32    4774

  Fragments arranged by size: 

                4774    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6319
 
PsiI TTA'TAA

Cuts at:   3078    3889    3078 
   Size:        811    5508
 
PstI C_TGCA'G

Cuts at:    830    3196    3671     830 
   Size:       2366     475    3478

  Fragments arranged by size: 

                3478    2366     475
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6319
 
SacI G_AGCT'C

Cuts at:   3583    3583 
   Size:       6319
 
SalI G'TCGA_C

Cuts at:   1936    3661    1936 
   Size:       1725    4594
 
SbfI CC_TGCA'GG

Cuts at:   3671    3671 
   Size:       6319
 
ScaI AGT'ACT

Cuts at:   2883    4567    6279    2883 
   Size:       1684    1712    2923

  Fragments arranged by size: 

                2923    1712    1684
 
SfcI C'TryA_G

Cuts at:    826    1045    2457    2697    3192    3279    3667    4802    5483 
   Size:        219    1412     240     495      87     388    1135     681

Cuts at:   5483    5674     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1412    1135     681     495     388     240     219
                 191      87
 
SfoI GGC'GCC

Cuts at:   3035    3035 
   Size:       6319
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6319
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6319
 
SmaI CCC'GGG

Cuts at:   1872    3646    1872 
   Size:       1774    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4423    5324    5586     820 
   Size:        197     926    2480     901     262    1553

  Fragments arranged by size: 

                2480    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4110     493 
   Size:       3617    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6319
 
SphI G_CATG'C

Cuts at:   1132    3677    1132 
   Size:       2545    3774
 
SspI AAT'ATT

Cuts at:      5      52    4243       5 
   Size:         47    4191    2081

  Fragments arranged by size: 

                4191    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6319
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3758     513 
   Size:        552     939    1754    3074

  Fragments arranged by size: 

                3074    1754     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4346    4363    4516    4701    5146     325 
   Size:       1255    2766      17     153     185     445    1498

  Fragments arranged by size: 

                2766    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2881    3182    4565 
   Size:        274      80      33      51    1443     904     301    1383

Cuts at:   4565    6277      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1383     904     301     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2205    2237    6279    6311    2205 
   Size:         32    4042      32    2213

  Fragments arranged by size: 

                4042    2213      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6319
 
XbaI T'CTAG_A

Cuts at:   3655    3655 
   Size:       6319
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6319
 
XmaI C'CCGG_G

Cuts at:   1870    3644    1870 
   Size:       1774    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2736    4448    2736 
   Size:       1712    4607
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4127     276 
   Size:         53      83     186     936    2593    2468

  Fragments arranged by size: 

                2593    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SacI     SalI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI   Bsu36I   DraIII EcoO109I    EcoRV
     FalI     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
    SacII    SanDI     SapI    SexAI     SfiI     SrfI     SwaI     XcmI
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