(Circular) (Six-base) MAPSORT of: pFN21AB9957.seq Check: 353 from: 1 to: 6319 pFN21AB9957 6319 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3320 3391 3320 Size: 71 6248 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2959 3169 3662 1937 Size: 1022 210 493 4594 Fragments arranged by size: 4594 1022 493 210 Acc65I G'GTAC_C Cuts at: 3262 3640 3262 Size: 378 5941 AclI AA'CG_TT Cuts at: 1563 3112 4446 4819 1563 Size: 1549 1334 373 3063 Fragments arranged by size: 3063 1549 1334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2199 2373 2429 3047 4382 5766 6034 Size: 516 51 174 56 618 1335 1384 268 Cuts at: 6034 1632 Size: 1917 Fragments arranged by size: 1917 1384 1335 618 516 268 174 56 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 2106 5150 2106 Size: 3044 3275 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3217 1184 Size: 2033 4286 AlwNI CAG_nnn'CTG Cuts at: 2035 3191 4062 5634 2035 Size: 1156 871 1572 2720 Fragments arranged by size: 2720 1572 1156 871 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2896 3048 3598 3628 3854 4006 6115 1493 Size: 1403 152 550 30 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 550 226 152 152 30 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 3644 1870 Size: 73 1701 4545 Fragments arranged by size: 4545 1701 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3033 3262 3640 5095 618 Size: 325 217 627 1246 229 378 1455 1842 Fragments arranged by size: 1842 1455 1246 627 378 325 229 217 BanII G_rGCy'C Cuts at: 1391 3367 3583 3638 1391 Size: 1976 216 55 4072 Fragments arranged by size: 4072 1976 216 55 BbeI G_GCGC'C Cuts at: 3037 3037 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3377 3385 928 Size: 971 1478 8 3862 Fragments arranged by size: 3862 1478 971 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2404 2545 6314 2404 Size: 141 3769 2409 Fragments arranged by size: 3769 2409 141 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 2446 4380 5536 1790 Size: 656 1934 1156 2573 Fragments arranged by size: 2573 1934 1156 656 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 4050 5008 Size: 416 53 83 399 24 882 1751 958 Cuts at: 5008 442 Size: 1753 Fragments arranged by size: 1753 1751 958 882 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2711 3447 3597 4979 1239 Size: 1472 736 150 1382 2579 Fragments arranged by size: 2579 1472 1382 736 150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2541 3017 3410 4982 882 Size: 617 1042 476 393 1572 2219 Fragments arranged by size: 2219 1572 1042 617 476 393 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3034 4126 4508 5143 Size: 53 83 186 936 1501 1092 382 635 Cuts at: 5143 275 Size: 1451 Fragments arranged by size: 1501 1451 1092 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2106 2448 4752 5150 5424 5571 5982 2106 Size: 342 2304 398 274 147 411 2443 Fragments arranged by size: 2443 2304 411 398 342 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6094 Fragments arranged by size: 6094 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6319 BseYI C'CCAG_C Cuts at: 3325 5522 6018 3325 Size: 2197 496 3626 Fragments arranged by size: 3626 2197 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5746 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2312 3583 4380 4465 5536 2312 Size: 1271 797 85 1071 3095 Fragments arranged by size: 3095 1271 1071 797 85 BsmI GAATG_Cn' Cuts at: 2364 3830 3923 2364 Size: 1466 93 4760 Fragments arranged by size: 4760 1466 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2446 3367 3583 3638 4380 4465 Size: 399 522 134 921 216 55 742 85 Cuts at: 4465 5536 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 921 742 522 399 216 134 85 55 BspEI T'CCGG_A Cuts at: 2448 5982 2448 Size: 3534 2785 BspHI T'CATG_A Cuts at: 2632 4208 5938 2632 Size: 1576 1730 3013 Fragments arranged by size: 3013 1730 1576 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3320 3391 3674 844 Size: 2476 71 283 3489 Fragments arranged by size: 3489 2476 283 71 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3204 3525 4814 4988 65 Size: 1183 1956 321 1289 174 1396 Fragments arranged by size: 1956 1396 1289 1183 321 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 4963 5150 1700 Size: 110 296 2857 187 2869 Fragments arranged by size: 2869 2857 296 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 3065 794 Size: 2271 4048 BstBI TT'CG_AA Cuts at: 3595 5123 3595 Size: 1528 4791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 3360 3360 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2404 2545 3321 3649 4016 4401 4418 5870 Size: 432 141 776 328 367 385 17 1452 Cuts at: 5870 5956 5968 6314 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 776 432 385 367 346 328 141 86 17 12 BstZ17I GTA'TAC Cuts at: 2960 3170 2960 Size: 210 6109 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 3835 4629 4649 3835 Size: 794 20 5505 Fragments arranged by size: 5505 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 2341 3623 2341 Size: 1282 5037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284 Size: 54 1425 50 275 18 1936 889 1629 Cuts at: 6284 8 Size: 43 Fragments arranged by size: 1936 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3354 4249 1353 Size: 2001 895 3423 Fragments arranged by size: 3423 2001 895 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3581 3581 Size: 6319 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2199 2373 2429 2711 3047 3447 Size: 393 516 51 174 56 282 336 400 Cuts at: 3447 3597 4382 4979 5766 6034 1239 Size: 150 785 597 787 268 1524 Fragments arranged by size: 1524 787 785 597 516 400 393 336 282 268 174 150 56 51 EcoNI CCTnn'n_nnAGG Cuts at: 2450 2450 Size: 6319 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 3037 5466 1678 Size: 1359 2429 2531 Fragments arranged by size: 2531 2429 1359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3349 3381 3445 3477 4960 Size: 32 135 32 1777 32 64 32 1483 Cuts at: 4960 4992 5034 5066 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1777 1483 135 64 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 2181 748 Size: 1433 4886 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 3033 3033 Size: 6319 KpnI G_GTAC'C Cuts at: 3266 3644 3266 Size: 378 5941 MfeI C'AATT_G Cuts at: 2592 3918 2592 Size: 1326 4993 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 3534 5433 5617 1305 Size: 1032 1197 1899 184 2007 Fragments arranged by size: 2007 1899 1197 1032 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 4277 4636 4795 518 Size: 648 924 2187 359 159 2042 Fragments arranged by size: 2187 2042 924 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3735 4412 5560 5805 1606 Size: 2129 677 1148 245 2120 Fragments arranged by size: 2129 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3034 3034 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2434 3553 3677 5222 1132 Size: 97 1205 1119 124 1545 2229 Fragments arranged by size: 2229 1545 1205 1119 124 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 3332 1108 Size: 2224 4095 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3078 3889 3078 Size: 811 5508 PstI C_TGCA'G Cuts at: 830 3196 3671 830 Size: 2366 475 3478 Fragments arranged by size: 3478 2366 475 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SacI G_AGCT'C Cuts at: 3583 3583 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 2883 4567 6279 2883 Size: 1684 1712 2923 Fragments arranged by size: 2923 1712 1684 SfcI C'TryA_G Cuts at: 826 1045 2457 2697 3192 3279 3667 4802 5483 Size: 219 1412 240 495 87 388 1135 681 Cuts at: 5483 5674 826 Size: 191 1471 Fragments arranged by size: 1471 1412 1135 681 495 388 240 219 191 87 SfoI GGC'GCC Cuts at: 3035 3035 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4423 5324 5586 820 Size: 197 926 2480 901 262 1553 Fragments arranged by size: 2480 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 3758 513 Size: 552 939 1754 3074 Fragments arranged by size: 3074 1754 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4346 4363 4516 4701 5146 325 Size: 1255 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2881 3182 4565 Size: 274 80 33 51 1443 904 301 1383 Cuts at: 4565 6277 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1383 904 301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 6279 6311 2205 Size: 32 4042 32 2213 Fragments arranged by size: 4042 2213 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 2736 4448 2736 Size: 1712 4607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI