Restriction map for
pFN21AB9957
(Linear) (Six-base) MAPSORT of: KIBB9957.seq Check: 7721 from: 1 to: 1614
KIBB9957.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1315 1386 1614
Size: 1315 71 228
Fragments arranged by size:
1315 228 71
AccI GT'mk_AC
Cuts at: 0 954 1164 1614
Size: 954 210 450
Fragments arranged by size:
954 450 210
Acc65I G'GTAC_C
Cuts at: 0 1257 1614
Size: 1257 357
AclI AA'CG_TT
Cuts at: 0 1107 1614
Size: 1107 507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 194 368 424 1042 1614
Size: 143 51 174 56 618 572
Fragments arranged by size:
618 572 174 143 56 51
AgeI A'CCGG_T
Cuts at: 0 101 1614
Size: 101 1513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1212 1614
Size: 1212 402
AlwNI CAG_nnn'CTG
Cuts at: 0 30 1186 1614
Size: 30 1156 428
Fragments arranged by size:
1156 428 30
ApoI r'AATT_y
Cuts at: 0 891 1043 1593 1614
Size: 891 152 550 21
Fragments arranged by size:
891 550 152 21
BanI G'GyrC_C
Cuts at: 0 1028 1257 1614
Size: 1028 229 357
Fragments arranged by size:
1028 357 229
BanII G_rGCy'C
Cuts at: 0 1362 1578 1614
Size: 1362 216 36
Fragments arranged by size:
1362 216 36
BbeI G_GCGC'C
Cuts at: 0 1032 1614
Size: 1032 582
BbsI GAAGACnn'nnnn_
Cuts at: 0 1372 1380 1614
Size: 1372 8 234
Fragments arranged by size:
1372 234 8
BglII A'GATC_T
Cuts at: 0 399 540 1614
Size: 399 141 1074
Fragments arranged by size:
1074 399 141
Bme1580I G_kGCm'C
Cuts at: 0 441 1614
Size: 441 1173
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 1614
Size: 294 1320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1442 1592 1614
Size: 706 736 150 22
Fragments arranged by size:
736 706 150 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 536 1012 1405 1614
Size: 536 476 393 209
Fragments arranged by size:
536 476 393 209
BsaHI Gr'CG_yC
Cuts at: 0 1029 1614
Size: 1029 585
BsaWI w'CCGG_w
Cuts at: 0 101 443 1614
Size: 101 342 1171
Fragments arranged by size:
1171 342 101
BseYI C'CCAG_C
Cuts at: 0 1320 1614
Size: 1320 294
BsiHKAI G_wGCw'C
Cuts at: 0 307 1578 1614
Size: 307 1271 36
Fragments arranged by size:
1271 307 36
BsmI GAATG_Cn'
Cuts at: 0 359 1614
Size: 359 1255
Bsp1286I G_dGCh'C
Cuts at: 0 307 441 1362 1578 1614
Size: 307 134 921 216 36
Fragments arranged by size:
921 307 216 134 36
BspEI T'CCGG_A
Cuts at: 0 443 1614
Size: 443 1171
BspHI T'CATG_A
Cuts at: 0 627 1614
Size: 627 987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1315 1386 1614
Size: 1315 71 228
Fragments arranged by size:
1315 228 71
BsrDI GCAATG_nn'
Cuts at: 0 1199 1520 1614
Size: 1199 321 94
Fragments arranged by size:
1199 321 94
BsrFI r'CCGG_y
Cuts at: 0 101 1614
Size: 101 1513
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1060 1614
Size: 1060 554
BstBI TT'CG_AA
Cuts at: 0 1590 1614
Size: 1590 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1355 1614
Size: 1355 259
BstYI r'GATC_y
Cuts at: 0 399 540 1316 1614
Size: 399 141 776 298
Fragments arranged by size:
776 399 298 141
BstZ17I GTA'TAC
Cuts at: 0 955 1165 1614
Size: 955 210 449
Fragments arranged by size:
955 449 210
DraI TTT'AAA
Cuts at: 0 336 1614
Size: 336 1278
EarI CTCTTCn'nnn_
Cuts at: 0 1349 1614
Size: 1349 265
EcoICRI GAG'CTC
Cuts at: 0 1576 1614
Size: 1576 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 194 368 424 706 1042 1442 1592
Size: 143 51 174 56 282 336 400 150
Cuts at: 1592 1614
Size: 22
Fragments arranged by size:
400 336 282 174 150 143 56 51
22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 445 1614
Size: 445 1169
HaeII r_GCGC'y
Cuts at: 0 1032 1614
Size: 1032 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1344 1376 1440 1472 1614
Size: 1344 32 64 32 142
Fragments arranged by size:
1344 142 64 32 32
HindIII A'AGCT_T
Cuts at: 0 176 1614
Size: 176 1438
KasI G'GCGC_C
Cuts at: 0 1028 1614
Size: 1028 586
KpnI G_GTAC'C
Cuts at: 0 1261 1614
Size: 1261 353
MfeI C'AATT_G
Cuts at: 0 587 1614
Size: 587 1027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 1529 1614
Size: 332 1197 85
Fragments arranged by size:
1197 332 85
MslI CAynn'nnrTG
Cuts at: 0 85 1614
Size: 85 1529
NarI GG'CG_CC
Cuts at: 0 1029 1614
Size: 1029 585
NspI r_CATG'y
Cuts at: 0 429 1548 1614
Size: 429 1119 66
Fragments arranged by size:
1119 429 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 1327 1614
Size: 1327 287
PsiI TTA'TAA
Cuts at: 0 1073 1614
Size: 1073 541
PstI C_TGCA'G
Cuts at: 0 1191 1614
Size: 1191 423
SacI G_AGCT'C
Cuts at: 0 1578 1614
Size: 1578 36
ScaI AGT'ACT
Cuts at: 0 878 1614
Size: 878 736
SfcI C'TryA_G
Cuts at: 0 452 692 1187 1274 1614
Size: 452 240 495 87 340
Fragments arranged by size:
495 452 340 240 87
SfoI GGC'GCC
Cuts at: 0 1030 1614
Size: 1030 584
TatI w'GTAC_w
Cuts at: 0 876 1177 1614
Size: 876 301 437
Fragments arranged by size:
876 437 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 1614
Size: 200 32 1382
Fragments arranged by size:
1382 200 32
XmnI GAAnn'nnTTC
Cuts at: 0 731 1614
Size: 731 883
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AgeI AlfI AlwNI
ApoI BanI BanII BbeI BbsI BglII Bme1580I BmrI
BpmI BsaI BsaHI BsaWI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BstAPI BstBI BstXI
BstYI BstZ17I DraI EarI EcoICRI Eco57MI EcoNI HaeII
Hin4I HindIII KasI KpnI MfeI MmeI MslI NarI
NspI PflMI PsiI PstI SacI ScaI SfcI SfoI
TatI TstI XmnI
Enzymes that do not cut:
AatII AfeI AflII AflIII AhdI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI
BsgI BsiEI BsiWI BsmBI BsrBI BsrGI BssHII BssSI
BstEII Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI MluI MscI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI