(Circular) (Six-base) MAPSORT of: pFN21AE4588.seq Check: 1498 from: 1 to: 7444 pFN21AE4588 7444 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5254 278 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4787 1937 Size: 2850 4594 Acc65I G'GTAC_C Cuts at: 4765 4765 Size: 7444 AclI AA'CG_TT Cuts at: 1563 3653 5571 5944 1563 Size: 2090 1918 373 3063 Fragments arranged by size: 3063 2090 1918 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2936 3222 3359 3427 3629 4100 4151 5507 Size: 1304 286 137 68 202 471 51 1356 Cuts at: 5507 6891 7159 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1356 1304 471 286 268 202 137 68 51 AflII C'TTAA_G Cuts at: 820 1017 4661 820 Size: 197 3644 3603 Fragments arranged by size: 3644 3603 197 AflIII A'CryG_T Cuts at: 1203 6343 7320 1203 Size: 5140 977 1327 Fragments arranged by size: 5140 1327 977 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7444 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3453 3485 3453 Size: 32 7412 AlwNI CAG_nnn'CTG Cuts at: 2099 2327 5187 6759 2099 Size: 228 2860 1572 2784 Fragments arranged by size: 2860 2784 1572 228 ApaLI G'TGCA_C Cuts at: 5501 6657 5501 Size: 1156 6288 ApoI r'AATT_y Cuts at: 1493 2956 3141 3411 3620 4208 4637 4753 4979 Size: 1463 185 270 209 588 429 116 226 Cuts at: 4979 5131 7240 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1463 588 429 270 226 209 185 152 116 AseI AT'TA_AT Cuts at: 160 1032 2492 3215 3323 3525 5998 160 Size: 872 1460 723 108 202 2473 1606 Fragments arranged by size: 2473 1606 1460 872 723 202 108 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4769 1870 Size: 73 2826 4545 Fragments arranged by size: 4545 2826 73 AvrII C'CTAG_G Cuts at: 4141 4141 Size: 7444 BamHI G'GATC_C Cuts at: 4774 5141 4774 Size: 367 7077 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4765 6220 618 Size: 325 217 627 2978 1455 1842 Fragments arranged by size: 2978 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3007 4763 1391 Size: 1616 1756 4072 Fragments arranged by size: 4072 1756 1616 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 2376 928 Size: 971 333 144 5996 Fragments arranged by size: 5996 971 333 144 BbvCI CC'TCA_GC Cuts at: 2092 2409 2092 Size: 317 7127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 5635 5669 1903 Size: 34 2120 34 1544 34 3678 Fragments arranged by size: 3678 2120 1544 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5336 6546 5336 Size: 1210 6234 BclI T'GATC_A Cuts at: 1548 2598 2904 2910 3019 3777 1548 Size: 1050 306 6 109 758 5215 Fragments arranged by size: 5215 1050 758 306 109 6 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6055 136 Size: 107 122 71 631 605 4383 1525 Fragments arranged by size: 4383 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2120 2790 7439 2120 Size: 670 4649 2125 Fragments arranged by size: 4649 2125 670 BlpI GC'TnA_GC Cuts at: 4861 4861 Size: 7444 Bme1580I G_kGCm'C Cuts at: 1790 5505 6661 1790 Size: 3715 1156 2573 Fragments arranged by size: 3715 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2079 5175 6133 Size: 416 53 83 399 24 662 3096 958 Cuts at: 6133 442 Size: 1753 Fragments arranged by size: 3096 1753 958 662 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2434 2434 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 3011 4217 6104 1239 Size: 1160 612 1206 1887 2579 Fragments arranged by size: 2579 1887 1206 1160 612 Bpu10I CC'TnA_GC Cuts at: 2092 2109 2409 2092 Size: 17 300 7127 Fragments arranged by size: 7127 300 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3773 5569 6434 6732 3773 Size: 1796 865 298 4485 Fragments arranged by size: 4485 1796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4243 6107 882 Size: 617 2744 1864 2219 Fragments arranged by size: 2744 2219 1864 617 BsaAI yAC'GTr Cuts at: 493 1204 2341 5235 493 Size: 711 1137 2894 2702 Fragments arranged by size: 2894 2702 1137 711 BsaBI GATnn'nnATC Cuts at: 2909 4469 7438 2909 Size: 1560 2969 2915 Fragments arranged by size: 2969 2915 1560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5251 5633 6268 275 Size: 53 83 186 936 3718 382 635 1451 Fragments arranged by size: 3718 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5877 6275 6549 6696 7107 5877 Size: 398 274 147 411 6214 Fragments arranged by size: 6214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3453 3483 1178 Size: 30 165 30 2050 30 5139 Fragments arranged by size: 5139 2050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2239 2250 2410 2413 2416 2419 2422 4298 Size: 1053 11 160 3 3 3 3 1876 Cuts at: 4298 1186 Size: 4332 Fragments arranged by size: 4332 1876 1053 160 11 3 3 3 3 BseYI C'CCAG_C Cuts at: 6647 7143 6647 Size: 496 6948 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3050 3741 1163 Size: 573 1314 691 4866 Fragments arranged by size: 4866 1314 691 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 5655 6683 1540 Size: 275 186 2893 761 1028 2301 Fragments arranged by size: 2893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5505 5590 6661 5505 Size: 85 1071 6288 Fragments arranged by size: 6288 1071 85 BsmI GAATG_Cn' Cuts at: 3051 3917 4955 5048 3051 Size: 866 1038 93 5447 Fragments arranged by size: 5447 1038 866 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2557 2557 Size: 7444 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3007 4763 5505 5590 6661 1391 Size: 399 1217 1756 742 85 1071 2174 Fragments arranged by size: 2174 1756 1217 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7107 7107 Size: 7444 BspHI T'CATG_A Cuts at: 2907 3978 5333 7063 2907 Size: 1071 1355 1730 3288 Fragments arranged by size: 3288 1730 1355 1071 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4799 844 Size: 3955 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5331 1394 Size: 429 3508 3507 Fragments arranged by size: 3508 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2890 5939 6113 65 Size: 1183 1642 3049 174 1396 Fragments arranged by size: 3049 1642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3186 6088 6275 1700 Size: 110 1376 2902 187 2869 Fragments arranged by size: 2902 2869 1376 187 110 BsrGI T'GTAC_A Cuts at: 96 2772 4623 96 Size: 2676 1851 2917 Fragments arranged by size: 2917 2676 1851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7444 BstBI TT'CG_AA Cuts at: 3109 4570 6248 3109 Size: 1461 1678 4305 Fragments arranged by size: 4305 1678 1461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7444 BstYI r'GATC_y Cuts at: 1972 2120 2790 4774 5141 5526 5543 6995 7081 Size: 148 670 1984 367 385 17 1452 86 Cuts at: 7081 7093 7439 1972 Size: 12 346 1977 Fragments arranged by size: 1984 1977 1452 670 385 367 346 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3546 513 Size: 552 2481 4411 Fragments arranged by size: 4411 2481 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 2679 3488 4346 4960 5754 5774 2679 Size: 809 858 614 794 20 4349 Fragments arranged by size: 4349 858 809 794 614 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4135 4170 529 Size: 35 3571 35 3803 Fragments arranged by size: 3803 3571 35 35 DraI TTT'AAA Cuts at: 2611 2862 3387 3571 3952 4213 4748 2611 Size: 251 525 184 381 261 535 5307 Fragments arranged by size: 5307 535 525 381 261 251 184 DraIII CAC_nnn'GTG Cuts at: 4195 4195 Size: 7444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6451 809 Size: 507 5135 1802 Fragments arranged by size: 5135 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4064 4891 5780 Size: 54 1425 50 275 18 2234 827 889 Cuts at: 5780 7409 8 Size: 1629 43 Fragments arranged by size: 2234 1629 1425 889 827 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4891 1537 Size: 275 3079 4090 Fragments arranged by size: 4090 3079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2248 2257 2503 3432 3446 4293 4661 Size: 812 83 9 246 929 14 847 368 Cuts at: 4661 5374 1353 Size: 713 3423 Fragments arranged by size: 3423 929 847 812 713 368 246 83 14 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2142 6031 6405 6551 387 Size: 1064 138 553 3889 374 146 1280 Fragments arranged by size: 3889 1280 1064 553 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2936 3011 3222 3359 3427 3629 Size: 393 767 537 75 211 137 68 202 Cuts at: 3629 4100 4151 4217 5507 6104 6891 7159 1239 Size: 471 51 66 1290 597 787 268 1524 Fragments arranged by size: 1524 1290 787 767 597 537 471 393 268 211 202 137 75 68 66 51 EcoRI G'AATT_C Cuts at: 2956 3411 4753 5131 2956 Size: 455 1342 378 5269 Fragments arranged by size: 5269 1342 455 378 EcoRV GAT'ATC Cuts at: 3598 3598 Size: 7444 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 3847 6591 1678 Size: 2169 2744 2531 Fragments arranged by size: 2744 2531 2169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 2225 2257 2270 Size: 32 135 32 533 32 88 32 13 Cuts at: 2270 2302 2889 2921 6085 6117 6159 6191 1373 Size: 32 587 32 3164 32 42 32 2626 Fragments arranged by size: 3164 2626 587 533 135 88 42 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3256 3583 4035 4179 4788 5034 Size: 988 281 1318 327 452 144 609 246 Cuts at: 5034 669 Size: 3079 Fragments arranged by size: 3079 1318 988 609 452 327 281 246 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 7444 HpaI GTT'AAC Cuts at: 3256 4035 5034 3256 Size: 779 999 5666 Fragments arranged by size: 5666 999 779 KpnI G_GTAC'C Cuts at: 4769 4769 Size: 7444 MfeI C'AATT_G Cuts at: 3042 3090 4563 5043 3042 Size: 48 1473 480 5443 Fragments arranged by size: 5443 1473 480 48 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3744 3835 6558 6742 1305 Size: 2439 91 2723 184 2007 Fragments arranged by size: 2723 2439 2007 184 91 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5622 Fragments arranged by size: 5622 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5402 5761 5920 518 Size: 648 4236 359 159 2042 Fragments arranged by size: 4236 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2782 3200 3683 4860 5537 6685 6930 Size: 490 686 418 483 1177 677 1148 245 Cuts at: 6930 1606 Size: 2120 Fragments arranged by size: 2120 1177 1148 686 677 490 483 418 245 NcoI C'CATG_G Cuts at: 513 1065 3546 513 Size: 552 2481 4411 Fragments arranged by size: 4411 2481 552 NdeI CA'TA_TG Cuts at: 387 4072 387 Size: 3685 3759 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NspI r_CATG'y Cuts at: 1132 1229 3127 4802 6347 1132 Size: 97 1898 1675 1545 2229 Fragments arranged by size: 2229 1898 1675 1545 97 PasI CC'CwG_GG Cuts at: 1348 2274 1348 Size: 926 6518 PciI A'CATG_T Cuts at: 6343 6343 Size: 7444 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7444 PfoI T'CCnGG_A Cuts at: 4018 4018 Size: 7444 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3447 3453 3479 3485 5513 5545 7026 7058 3447 Size: 6 26 6 2028 32 1481 32 3833 Fragments arranged by size: 3833 2028 1481 32 32 26 6 6 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7444 PsiI TTA'TAA Cuts at: 3014 5014 3014 Size: 2000 5444 PstI C_TGCA'G Cuts at: 830 4353 4796 830 Size: 3523 443 3478 Fragments arranged by size: 3523 3478 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 2096 2782 3200 3683 2096 Size: 686 418 483 5857 Fragments arranged by size: 5857 686 483 418 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SapI GCTCTTCn'nnn_ Cuts at: 3446 3446 Size: 7444 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 2695 5692 7404 2695 Size: 2997 1712 2735 Fragments arranged by size: 2997 2735 1712 SfcI C'TryA_G Cuts at: 826 1045 2733 3333 3738 3870 4349 4518 4792 Size: 219 1688 600 405 132 479 169 274 Cuts at: 4792 5927 6608 6799 826 Size: 1135 681 191 1471 Fragments arranged by size: 1688 1471 1135 681 600 479 405 274 219 191 169 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4771 1872 Size: 2899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3752 4661 5548 6449 6711 820 Size: 197 926 1809 909 887 901 262 1553 Fragments arranged by size: 1809 1553 926 909 901 887 262 197 SnaBI TAC'GTA Cuts at: 493 5235 493 Size: 4742 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 3127 4802 1132 Size: 1995 1675 3774 Fragments arranged by size: 3774 1995 1675 SspI AAT'ATT Cuts at: 5 52 4027 5368 5 Size: 47 3975 1341 2081 Fragments arranged by size: 3975 2081 1341 47 StuI AGG'CCT Cuts at: 1517 3818 1517 Size: 2301 5143 StyI C'CwwG_G Cuts at: 513 1065 3546 4141 4883 513 Size: 552 2481 595 742 3074 Fragments arranged by size: 3074 2481 742 595 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3326 5471 5488 5641 5826 6271 325 Size: 1255 1746 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1746 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2693 2706 2772 Size: 274 80 33 51 1443 716 13 66 Cuts at: 2772 4623 4728 5690 7402 96 Size: 1851 105 962 1712 138 Fragments arranged by size: 1851 1712 1443 962 716 274 138 105 80 66 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4179 4211 7404 7436 4179 Size: 32 3193 32 4187 Fragments arranged by size: 4187 3193 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7444 XbaI T'CTAG_A Cuts at: 3265 4780 3265 Size: 1515 5929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 2980 Size: 7444 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4769 1870 Size: 2899 4545 XmnI GAAnn'nnTTC Cuts at: 2682 5573 2682 Size: 2891 4553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5252 276 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI