Restriction map for
pFN21AE6336
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
Ksp632I KspI MaeIII MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI TfiI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1178 CGCG CG^CG
AcsI 784 GAATTT [GA]^AATT[CT]
AcyI 1090 GACGCC G[GA]^CG[CT]C
AgeI 215 ACCGGT A^CCGGT
AocI 1216 CCTGAGG CC^T[ACGT]AGG
ApoI 784 GAATTT [GA]^AATT[CT]
AvaI 125 CCCGGG C^[CT]CG[GA]G
AviII 911 TGCGCA TGC^GCA
BanI 893 GGCACC G^G[CT][GA]CC
BanII 832 GAGCTC G[GA]GC[CT]^C
BbrPI 617 CACGTG CAC^GTG
BbvCI 714 GCTGAGG CCTCAGC(-5/-2)
BcgI 261 CGAGATCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 1090 GACGCC G[GA]^CG[CT]C
BsaWI 215 ACCGGT [AT]^CCGG[AT]
BsoBI 125 CCCGGG C^[CT]CG[GA]G
Bsp1407I 824 TGTACA T^GTACA
BspDI 265 ATCGAT AT^CGAT
BsrBI 683 GAGCGG CCGCTC(-3/-3)
BsrDI 1128 CATTGC GCAATG(2/0)
BsrGI 824 TGTACA T^GTACA
BstAPI 300 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1178 CGCG CG^CG
Bsu36I 1216 CCTGAGG CC^T[ACGT]AGG
ClaI 265 ATCGAT AT^CGAT
DraII 389 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1161 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1216 CCTGAGG CC^T[ACGT]AGG
EcoO109I 389 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 857 CCTTGG C^C[AT][AT]GG
FspI 911 TGCGCA TGC^GCA
HaeII 29 GGCGCT [GA]GCGC^[CT]
Hin1I 1090 GACGCC G[GA]^CG[CT]C
MboII 849 GAAGA GAAGA(8/7)
MslI 375 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1178 CGCG CG^CG
NaeI 365 GCCGGC GCC^GGC
NgoMIV 365 GCCGGC G^CCGGC
PinAI 215 ACCGGT A^CCGGT
PmaCI 617 CACGTG CAC^GTG
PmlI 617 CACGTG CAC^GTG
PpuMI 389 AGGACCC [GA]G^G[AT]CC[CT]
SacI 832 GAGCTC GAGCT^C
SbfI 306 CCTGCAGG CCTGCA^GG
SmaI 125 CCCGGG CCC^GGG
SmlI 835 CTCAAG C^T[CT][GA]AG
Sse8387I 306 CCTGCAGG CCTGCA^GG
SspBI 824 TGTACA T^GTACA
StyI 857 CCTTGG C^C[AT][AT]GG
Tsp509I 785 AATT ^AATT
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 558 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1060 CAGAAGCTG
AspHI 130 GTGCTC G[AT]GC[AT]^C
832 GAGCTC
BalI 2 TGGCCA TGG^CCA
335 TGGCCA
BlpI 9 GCTGAGC GC^T[ACGT]AGC
871 GCTGAGC
BmyI 130 GTGCTC G[AGT]GC[ACT]^C
832 GAGCTC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
871 GCTGAGC
BsaAI 617 CACGTG [CT]AC^GT[GA]
1235 CACGTA
BsaI 142 GAGACC GGTCTC(1/5)
745 GAGACC
BsaJI 125 CCCGGG C^C[ACGT][ACGT]GG
857 CCTTGG
BseRI 862 GAGGAG GAGGAG(10/8)
901 GAGGAG
BsiHKAI 130 GTGCTC G[AT]GC[AT]^C
832 GAGCTC
BsmFI 992 GGGAC GGGAC(10/14)
939 GTCCC
Bsp1286I 130 GTGCTC G[AGT]GC[ACT]^C
832 GAGCTC
BstXI 1148 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1163 CCAAGTGCATGG
CelII 9 GCTGAGC GC^T[ACGT]AGC
871 GCTGAGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
335 TGGCCA
EcoNI 489 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
897 CCTGGAGGAGG
FauI 357 GCGGG CCCGC(4/6)
807 GCGGG
HincII 211 GTCAAC GT[CT]^[GA]AC
607 GTTGAC
HindII 211 GTCAAC GT[CT]^[GA]AC
607 GTTGAC
MluNI 2 TGGCCA TGG^CCA
335 TGGCCA
MscI 2 TGGCCA TGG^CCA
335 TGGCCA
PshAI 235 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
658 GACTTCAGTC
StuI 446 AGGCCT AGG^CCT
470 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BbvI BcnI BfaI BglI
BpmI BsiYI BslI BsmAI BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MboI MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I
XhoII