(Circular) (Six-base) MAPSORT of: pFN21AE6336.seq Check: 8093 from: 1 to: 5995 pFN21AE6336 5995 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2650 2782 2957 3080 4058 5442 5710 Size: 995 23 132 175 123 978 1384 268 Cuts at: 5710 1632 Size: 1917 Fragments arranged by size: 1917 1384 995 978 268 175 132 123 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 2621 2623 3239 4894 5871 1203 Size: 1418 2 616 1655 977 1327 Fragments arranged by size: 1655 1418 1327 977 616 2 AgeI A'CCGG_T Cuts at: 2220 4826 2220 Size: 2606 3389 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2118 1184 Size: 934 5061 AlwNI CAG_nnn'CTG Cuts at: 2568 3070 3738 5310 2568 Size: 502 668 1572 3253 Fragments arranged by size: 3253 1572 668 502 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 2789 3304 3530 3682 5791 1493 Size: 1296 515 226 152 2109 1697 Fragments arranged by size: 2109 1697 1296 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2130 3320 1870 Size: 73 187 1190 4545 Fragments arranged by size: 4545 1190 187 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2610 2643 2610 Size: 33 5962 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2898 3316 4771 618 Size: 325 217 627 1111 418 1455 1842 Fragments arranged by size: 1842 1455 1111 627 418 325 217 BanII G_rGCy'C Cuts at: 1391 2841 3314 1391 Size: 1450 473 4072 Fragments arranged by size: 4072 1450 473 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BbvCI CC'TCA_GC Cuts at: 2720 2720 Size: 5995 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2255 2289 4186 4220 1903 Size: 34 318 34 1897 34 3678 Fragments arranged by size: 3678 1897 318 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2032 2053 3143 Size: 107 122 71 631 605 360 21 1090 Cuts at: 3143 4606 136 Size: 1463 1525 Fragments arranged by size: 1525 1463 1090 631 605 360 122 107 71 21 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 2015 2877 3412 2015 Size: 862 535 4598 Fragments arranged by size: 4598 862 535 Bme1580I G_kGCm'C Cuts at: 1790 4056 5212 1790 Size: 2266 1156 2573 Fragments arranged by size: 2573 2266 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2588 2588 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 2924 3305 4655 1239 Size: 1394 291 381 1350 2579 Fragments arranged by size: 2579 1394 1350 381 291 Bpu10I CC'TnA_GC Cuts at: 2720 2930 2720 Size: 210 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 4120 4985 5283 2825 Size: 1295 865 298 3537 Fragments arranged by size: 3537 1295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2141 2744 4658 882 Size: 617 642 603 1914 2219 Fragments arranged by size: 2219 1914 642 617 603 BsaAI yAC'GTr Cuts at: 493 1204 2624 3242 3786 493 Size: 711 1420 618 544 2702 Fragments arranged by size: 2702 1420 711 618 544 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3096 3802 4184 4819 Size: 53 83 186 936 1563 706 382 635 Cuts at: 4819 275 Size: 1451 Fragments arranged by size: 1563 1451 936 706 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2220 4428 4826 5100 5247 5658 2220 Size: 2208 398 274 147 411 2557 Fragments arranged by size: 2557 2208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2813 2843 1178 Size: 30 165 30 1410 30 4330 Fragments arranged by size: 4330 1410 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2882 2921 1186 Size: 1696 39 4260 Fragments arranged by size: 4260 1696 39 BseYI C'CCAG_C Cuts at: 2088 5198 5694 2088 Size: 3110 496 2389 Fragments arranged by size: 3110 2389 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2841 4056 4141 5212 2139 Size: 702 1215 85 1071 2922 Fragments arranged by size: 2922 1215 1071 702 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2841 3314 4056 4141 5212 1391 Size: 399 349 702 473 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 473 399 349 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2319 2334 3350 844 Size: 1235 240 15 1016 3489 Fragments arranged by size: 3489 1235 1016 240 15 BsrBI CCG'CTC Cuts at: 1394 1823 2690 3882 1394 Size: 429 867 1192 3507 Fragments arranged by size: 3507 1192 867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3132 4490 4664 65 Size: 1183 1884 1358 174 1396 Fragments arranged by size: 1884 1396 1358 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2220 2370 2973 4639 4826 1700 Size: 110 410 150 603 1666 187 2869 Fragments arranged by size: 2869 1666 603 410 187 150 110 BsrGI T'GTAC_A Cuts at: 96 2829 96 Size: 2733 3262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 2311 794 Size: 1517 4478 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstXI CCAn_nnnn'nTGG Cuts at: 3160 3175 3160 Size: 15 5980 BstYI r'GATC_y Cuts at: 1972 2115 2588 2911 3325 3692 4077 4094 5546 Size: 143 473 323 414 367 385 17 1452 Cuts at: 5546 5632 5644 5990 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 473 414 385 367 346 323 143 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3222 3222 Size: 5995 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3034 487 Size: 683 56 1808 3448 Fragments arranged by size: 3448 1808 683 56 BtsI GCAGTG_nn' Cuts at: 2660 2730 3511 4305 4325 2660 Size: 70 781 794 20 4330 Fragments arranged by size: 4330 794 781 70 20 ClaI AT'CG_AT Cuts at: 1554 2271 1554 Size: 717 5278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DraIII CAC_nnn'GTG Cuts at: 3171 3171 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2340 3291 Size: 54 1425 50 275 18 177 333 951 Cuts at: 3291 3442 4331 5960 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 951 889 333 275 177 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3925 1353 Size: 2572 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2108 4582 4956 5102 387 Size: 1064 138 519 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 519 374 146 138 EcoICRI GAG'CTC Cuts at: 2839 2839 Size: 5995 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2627 2633 2650 2782 2924 2957 3080 Size: 393 995 6 17 132 142 33 123 Cuts at: 3080 3305 4058 4655 5442 5710 1239 Size: 225 753 597 787 268 1524 Fragments arranged by size: 1524 995 787 753 597 393 268 225 142 132 123 33 17 6 EcoNI CCTnn'n_nnAGG Cuts at: 2498 2906 2498 Size: 408 5587 EcoO109I rG'GnC_Cy Cuts at: 2395 2395 Size: 5995 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2470 2878 2470 Size: 408 5587 FspI TGC'GCA Cuts at: 2918 4501 2918 Size: 1583 4412 HaeII r_GCGC'y Cuts at: 1678 2038 5142 1678 Size: 360 3104 2531 Fragments arranged by size: 3104 2531 360 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373 Size: 32 135 32 3064 32 42 32 2626 Fragments arranged by size: 3064 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2218 2614 3339 3585 669 Size: 988 281 280 396 725 246 3079 Fragments arranged by size: 3079 988 725 396 281 280 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2533 2550 5109 5293 1305 Size: 1228 17 2559 184 2007 Fragments arranged by size: 2559 2007 1228 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2342 10 Size: 54 1425 343 177 333 3663 Fragments arranged by size: 3663 1425 343 333 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 3953 4312 4471 518 Size: 648 1218 1569 359 159 2042 Fragments arranged by size: 2042 1569 1218 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2092 3283 3411 4088 5236 5481 1606 Size: 438 48 1191 128 677 1148 245 2120 Fragments arranged by size: 2120 1191 1148 677 438 245 128 48 NaeI GCC'GGC Cuts at: 2372 2372 Size: 5995 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NgoMIV G'CCGG_C Cuts at: 2370 2370 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3353 4898 1132 Size: 97 2124 1545 2229 Fragments arranged by size: 2229 2124 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PmlI CAC'GTG Cuts at: 2624 2624 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2395 Size: 5995 PshAI GACnn'nnGTC Cuts at: 1613 2244 2667 1613 Size: 631 423 4941 Fragments arranged by size: 4941 631 423 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PstI C_TGCA'G Cuts at: 830 2097 2316 2886 3126 3347 830 Size: 1267 219 570 240 221 3478 Fragments arranged by size: 3478 1267 570 240 221 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2044 2092 3283 2044 Size: 48 1191 4756 Fragments arranged by size: 4756 1191 48 SacI G_AGCT'C Cuts at: 2841 2841 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SbfI CC_TGCA'GG Cuts at: 2316 3347 2316 Size: 1031 4964 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2093 2312 2882 3122 3343 4478 5159 Size: 219 1048 219 570 240 221 1135 681 Cuts at: 5159 5350 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1048 681 570 240 221 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 2132 3322 1872 Size: 260 1190 4545 Fragments arranged by size: 4545 1190 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2840 4099 5000 5262 820 Size: 197 926 897 1259 901 262 1553 Fragments arranged by size: 1553 1259 926 901 897 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 2453 2477 1517 Size: 936 24 5035 Fragments arranged by size: 5035 936 24 StyI C'CwwG_G Cuts at: 513 1065 2004 2862 3434 513 Size: 552 939 858 572 3074 Fragments arranged by size: 3074 939 858 572 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2700 4022 4039 4192 4377 4822 325 Size: 1255 1120 1322 17 153 185 445 1498 Fragments arranged by size: 1498 1322 1255 1120 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 4241 5953 Size: 274 80 33 51 1443 852 1412 1712 Cuts at: 5953 96 Size: 138 Fragments arranged by size: 1712 1443 1412 852 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 3155 3187 3247 3279 5955 5987 3086 Size: 32 37 32 60 32 2676 32 3094 Fragments arranged by size: 3094 2676 60 37 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 2130 3320 1870 Size: 260 1190 4545 Fragments arranged by size: 4545 1190 260 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI