Restriction map for pFN21AE6336
 (Linear) (Six-base) MAPSORT of: KIEE6336.seq  Check: 965  from: 1  to: 1290



KIEE6336.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622     645     777     952    1075    1290 
   Size:        622      23     132     175     123     215

  Fragments arranged by size: 

                 622     215     175     132     123      23
 
AflIII A'CryG_T

Cuts at:      0     616     618    1234    1290 
   Size:        616       2     616      56

  Fragments arranged by size: 

                 616     616      56       2
 
AgeI A'CCGG_T

Cuts at:      0     215    1290 
   Size:        215    1075
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     113    1290 
   Size:        113    1177
 
AlwNI CAG_nnn'CTG

Cuts at:      0     563    1065    1290 
   Size:        563     502     225

  Fragments arranged by size: 

                 563     502     225
 
ApoI r'AATT_y

Cuts at:      0     784    1290 
   Size:        784     506
 
AvaI C'yCGr_G

Cuts at:      0     125    1290 
   Size:        125    1165
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     605     638    1290 
   Size:        605      33     652

  Fragments arranged by size: 

                 652     605      33
 
BanI G'GyrC_C

Cuts at:      0     893    1290 
   Size:        893     397
 
BanII G_rGCy'C

Cuts at:      0     836    1290 
   Size:        836     454
 
BbvCI CC'TCA_GC

Cuts at:      0     715    1290 
   Size:        715     575
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     250     284    1290 
   Size:        250      34    1006

  Fragments arranged by size: 

                1006     250      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      27      48    1138    1290 
   Size:         27      21    1090     152

  Fragments arranged by size: 

                1090     152      27      21
 
BlpI GC'TnA_GC

Cuts at:      0      10     872    1290 
   Size:         10     862     418

  Fragments arranged by size: 

                 862     418      10
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     583    1290 
   Size:        583     707
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     919    1290 
   Size:        628     291     371

  Fragments arranged by size: 

                 628     371     291
 
Bpu10I CC'TnA_GC

Cuts at:      0     715     925    1290 
   Size:        715     210     365

  Fragments arranged by size: 

                 715     365     210
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1290 
   Size:        820     470
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     136     739    1290 
   Size:        136     603     551

  Fragments arranged by size: 

                 603     551     136
 
BsaAI yAC'GTr

Cuts at:      0     619    1237    1290 
   Size:        619     618      53

  Fragments arranged by size: 

                 619     618      53
 
BsaHI Gr'CG_yC

Cuts at:      0    1091    1290 
   Size:       1091     199
 
BsaWI w'CCGG_w

Cuts at:      0     215    1290 
   Size:        215    1075
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     808     838    1290 
   Size:        808      30     452

  Fragments arranged by size: 

                 808     452      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     877     916    1290 
   Size:        877      39     374

  Fragments arranged by size: 

                 877     374      39
 
BseYI C'CCAG_C

Cuts at:      0      83    1290 
   Size:         83    1207
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     836    1290 
   Size:        134     702     454

  Fragments arranged by size: 

                 702     454     134
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     836    1290 
   Size:        134     702     454

  Fragments arranged by size: 

                 702     454     134
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     314     329    1290 
   Size:         74     240      15     961

  Fragments arranged by size: 

                 961     240      74      15
 
BsrBI CCG'CTC

Cuts at:      0     685    1290 
   Size:        685     605
 
BsrDI GCAATG_nn'

Cuts at:      0    1127    1290 
   Size:       1127     163
 
BsrFI r'CCGG_y

Cuts at:      0     215     365     968    1290 
   Size:        215     150     603     322

  Fragments arranged by size: 

                 603     322     215     150
 
BsrGI T'GTAC_A

Cuts at:      0     824    1290 
   Size:        824     466
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     306    1290 
   Size:        306     984
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1155    1170    1290 
   Size:       1155      15     120

  Fragments arranged by size: 

                1155     120      15
 
BstYI r'GATC_y

Cuts at:      0     110     583     906    1290 
   Size:        110     473     323     384

  Fragments arranged by size: 

                 473     384     323     110
 
Bsu36I CC'TnA_GG

Cuts at:      0    1217    1290 
   Size:       1217      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1029    1290 
   Size:       1029     261
 
BtsI GCAGTG_nn'

Cuts at:      0     655     725    1290 
   Size:        655      70     565

  Fragments arranged by size: 

                 655     565      70
 
ClaI AT'CG_AT

Cuts at:      0     266    1290 
   Size:        266    1024
 
DraIII CAC_nnn'GTG

Cuts at:      0    1166    1290 
   Size:       1166     124
 
EaeI y'GGCC_r

Cuts at:      0       2     335    1290 
   Size:          2     333     955

  Fragments arranged by size: 

                 955     333       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     103    1290 
   Size:        103    1187
 
EcoICRI GAG'CTC

Cuts at:      0     834    1290 
   Size:        834     456
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622     628     645     777     919     952    1075    1290 
   Size:        622       6      17     132     142      33     123     215

  Fragments arranged by size: 

                 622     215     142     132     123      33      17       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     493     901    1290 
   Size:        493     408     389

  Fragments arranged by size: 

                 493     408     389
 
EcoO109I rG'GnC_Cy

Cuts at:      0     390    1290 
   Size:        390     900
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     465     873    1290 
   Size:        465     408     417

  Fragments arranged by size: 

                 465     417     408
 
FspI TGC'GCA

Cuts at:      0     913    1290 
   Size:        913     377
 
HaeII r_GCGC'y

Cuts at:      0      33    1290 
   Size:         33    1257
 
HincII GTy'rAC

Cuts at:      0     213     609    1290 
   Size:        213     396     681

  Fragments arranged by size: 

                 681     396     213
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     528     545    1290 
   Size:        528      17     745

  Fragments arranged by size: 

                 745     528      17
 
MscI TGG'CCA

Cuts at:      0       4     337    1290 
   Size:          4     333     953

  Fragments arranged by size: 

                 953     333       4
 
MslI CAynn'nnrTG

Cuts at:      0     379    1290 
   Size:        379     911
 
MspA1I CmG'CkG

Cuts at:      0      39      87    1278    1290 
   Size:         39      48    1191      12

  Fragments arranged by size: 

                1191      48      39      12
 
NaeI GCC'GGC

Cuts at:      0     367    1290 
   Size:        367     923
 
NgoMIV G'CCGG_C

Cuts at:      0     365    1290 
   Size:        365     925
 
PmlI CAC'GTG

Cuts at:      0     619    1290 
   Size:        619     671
 
PpuMI rG'GwC_Cy

Cuts at:      0     390    1290 
   Size:        390     900
 
PshAI GACnn'nnGTC

Cuts at:      0     239     662    1290 
   Size:        239     423     628

  Fragments arranged by size: 

                 628     423     239
 
PstI C_TGCA'G

Cuts at:      0      92     311     881    1121    1290 
   Size:         92     219     570     240     169

  Fragments arranged by size: 

                 570     240     219     169      92
 
PvuII CAG'CTG

Cuts at:      0      39      87    1278    1290 
   Size:         39      48    1191      12

  Fragments arranged by size: 

                1191      48      39      12
 
SacI G_AGCT'C

Cuts at:      0     836    1290 
   Size:        836     454
 
SbfI CC_TGCA'GG

Cuts at:      0     311    1290 
   Size:        311     979
 
SfcI C'TryA_G

Cuts at:      0      88     307     877    1117    1290 
   Size:         88     219     570     240     173

  Fragments arranged by size: 

                 570     240     219     173      88
 
SmaI CCC'GGG

Cuts at:      0     127    1290 
   Size:        127    1163
 
SmlI C'TyrA_G

Cuts at:      0     835    1290 
   Size:        835     455
 
StuI AGG'CCT

Cuts at:      0     448     472    1290 
   Size:        448      24     818

  Fragments arranged by size: 

                 818     448      24
 
StyI C'CwwG_G

Cuts at:      0     857    1290 
   Size:        857     433
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     695    1290 
   Size:        695     595
 
TatI w'GTAC_w

Cuts at:      0     824    1290 
   Size:        824     466
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1081    1113    1150    1182    1242    1274    1290 
   Size:       1081      32      37      32      60      32      16

  Fragments arranged by size: 

                1081      60      37      32      32      32      16
 
XmaI C'CCGG_G

Cuts at:      0     125    1290 
   Size:        125    1165
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AlfI    AlwNI     ApoI     AvaI     BaeI
     BanI    BanII    BbvCI     BcgI     BglI     BlpI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I    BtgZI     BtsI     ClaI   DraIII
     EaeI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII   HincII     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV
     PmlI    PpuMI    PshAI     PstI    PvuII     SacI     SbfI     SfcI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BbeI     BbsI    BciVI     BclI    BfrBI    BglII Bme1580I
    BmgBI     BmrI     BmtI    BsaBI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI   BssHII    BssSI    BstBI   BstEII  BstZ17I
     BtgI    CspCI     DraI     DrdI     EagI     EarI    EcoRI    EcoRV
     FseI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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