(Linear) (Six-base) MAPSORT of: KIEE6336.seq Check: 965 from: 1 to: 1290 KIEE6336.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 645 777 952 1075 1290 Size: 622 23 132 175 123 215 Fragments arranged by size: 622 215 175 132 123 23 AflIII A'CryG_T Cuts at: 0 616 618 1234 1290 Size: 616 2 616 56 Fragments arranged by size: 616 616 56 2 AgeI A'CCGG_T Cuts at: 0 215 1290 Size: 215 1075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 113 1290 Size: 113 1177 AlwNI CAG_nnn'CTG Cuts at: 0 563 1065 1290 Size: 563 502 225 Fragments arranged by size: 563 502 225 ApoI r'AATT_y Cuts at: 0 784 1290 Size: 784 506 AvaI C'yCGr_G Cuts at: 0 125 1290 Size: 125 1165 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 605 638 1290 Size: 605 33 652 Fragments arranged by size: 652 605 33 BanI G'GyrC_C Cuts at: 0 893 1290 Size: 893 397 BanII G_rGCy'C Cuts at: 0 836 1290 Size: 836 454 BbvCI CC'TCA_GC Cuts at: 0 715 1290 Size: 715 575 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 250 284 1290 Size: 250 34 1006 Fragments arranged by size: 1006 250 34 BglI GCCn_nnn'nGGC Cuts at: 0 27 48 1138 1290 Size: 27 21 1090 152 Fragments arranged by size: 1090 152 27 21 BlpI GC'TnA_GC Cuts at: 0 10 872 1290 Size: 10 862 418 Fragments arranged by size: 862 418 10 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 583 1290 Size: 583 707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 919 1290 Size: 628 291 371 Fragments arranged by size: 628 371 291 Bpu10I CC'TnA_GC Cuts at: 0 715 925 1290 Size: 715 210 365 Fragments arranged by size: 715 365 210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 1290 Size: 820 470 BsaI GGTCTCn'nnnn_ Cuts at: 0 136 739 1290 Size: 136 603 551 Fragments arranged by size: 603 551 136 BsaAI yAC'GTr Cuts at: 0 619 1237 1290 Size: 619 618 53 Fragments arranged by size: 619 618 53 BsaHI Gr'CG_yC Cuts at: 0 1091 1290 Size: 1091 199 BsaWI w'CCGG_w Cuts at: 0 215 1290 Size: 215 1075 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 808 838 1290 Size: 808 30 452 Fragments arranged by size: 808 452 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 877 916 1290 Size: 877 39 374 Fragments arranged by size: 877 374 39 BseYI C'CCAG_C Cuts at: 0 83 1290 Size: 83 1207 BsiHKAI G_wGCw'C Cuts at: 0 134 836 1290 Size: 134 702 454 Fragments arranged by size: 702 454 134 Bsp1286I G_dGCh'C Cuts at: 0 134 836 1290 Size: 134 702 454 Fragments arranged by size: 702 454 134 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 74 314 329 1290 Size: 74 240 15 961 Fragments arranged by size: 961 240 74 15 BsrBI CCG'CTC Cuts at: 0 685 1290 Size: 685 605 BsrDI GCAATG_nn' Cuts at: 0 1127 1290 Size: 1127 163 BsrFI r'CCGG_y Cuts at: 0 215 365 968 1290 Size: 215 150 603 322 Fragments arranged by size: 603 322 215 150 BsrGI T'GTAC_A Cuts at: 0 824 1290 Size: 824 466 BstAPI GCAn_nnn'nTGC Cuts at: 0 306 1290 Size: 306 984 BstXI CCAn_nnnn'nTGG Cuts at: 0 1155 1170 1290 Size: 1155 15 120 Fragments arranged by size: 1155 120 15 BstYI r'GATC_y Cuts at: 0 110 583 906 1290 Size: 110 473 323 384 Fragments arranged by size: 473 384 323 110 Bsu36I CC'TnA_GG Cuts at: 0 1217 1290 Size: 1217 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1029 1290 Size: 1029 261 BtsI GCAGTG_nn' Cuts at: 0 655 725 1290 Size: 655 70 565 Fragments arranged by size: 655 565 70 ClaI AT'CG_AT Cuts at: 0 266 1290 Size: 266 1024 DraIII CAC_nnn'GTG Cuts at: 0 1166 1290 Size: 1166 124 EaeI y'GGCC_r Cuts at: 0 2 335 1290 Size: 2 333 955 Fragments arranged by size: 955 333 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 103 1290 Size: 103 1187 EcoICRI GAG'CTC Cuts at: 0 834 1290 Size: 834 456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 628 645 777 919 952 1075 1290 Size: 622 6 17 132 142 33 123 215 Fragments arranged by size: 622 215 142 132 123 33 17 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 493 901 1290 Size: 493 408 389 Fragments arranged by size: 493 408 389 EcoO109I rG'GnC_Cy Cuts at: 0 390 1290 Size: 390 900 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 465 873 1290 Size: 465 408 417 Fragments arranged by size: 465 417 408 FspI TGC'GCA Cuts at: 0 913 1290 Size: 913 377 HaeII r_GCGC'y Cuts at: 0 33 1290 Size: 33 1257 HincII GTy'rAC Cuts at: 0 213 609 1290 Size: 213 396 681 Fragments arranged by size: 681 396 213 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 528 545 1290 Size: 528 17 745 Fragments arranged by size: 745 528 17 MscI TGG'CCA Cuts at: 0 4 337 1290 Size: 4 333 953 Fragments arranged by size: 953 333 4 MslI CAynn'nnrTG Cuts at: 0 379 1290 Size: 379 911 MspA1I CmG'CkG Cuts at: 0 39 87 1278 1290 Size: 39 48 1191 12 Fragments arranged by size: 1191 48 39 12 NaeI GCC'GGC Cuts at: 0 367 1290 Size: 367 923 NgoMIV G'CCGG_C Cuts at: 0 365 1290 Size: 365 925 PmlI CAC'GTG Cuts at: 0 619 1290 Size: 619 671 PpuMI rG'GwC_Cy Cuts at: 0 390 1290 Size: 390 900 PshAI GACnn'nnGTC Cuts at: 0 239 662 1290 Size: 239 423 628 Fragments arranged by size: 628 423 239 PstI C_TGCA'G Cuts at: 0 92 311 881 1121 1290 Size: 92 219 570 240 169 Fragments arranged by size: 570 240 219 169 92 PvuII CAG'CTG Cuts at: 0 39 87 1278 1290 Size: 39 48 1191 12 Fragments arranged by size: 1191 48 39 12 SacI G_AGCT'C Cuts at: 0 836 1290 Size: 836 454 SbfI CC_TGCA'GG Cuts at: 0 311 1290 Size: 311 979 SfcI C'TryA_G Cuts at: 0 88 307 877 1117 1290 Size: 88 219 570 240 173 Fragments arranged by size: 570 240 219 173 88 SmaI CCC'GGG Cuts at: 0 127 1290 Size: 127 1163 SmlI C'TyrA_G Cuts at: 0 835 1290 Size: 835 455 StuI AGG'CCT Cuts at: 0 448 472 1290 Size: 448 24 818 Fragments arranged by size: 818 448 24 StyI C'CwwG_G Cuts at: 0 857 1290 Size: 857 433 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 695 1290 Size: 695 595 TatI w'GTAC_w Cuts at: 0 824 1290 Size: 824 466 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1081 1113 1150 1182 1242 1274 1290 Size: 1081 32 37 32 60 32 16 Fragments arranged by size: 1081 60 37 32 32 32 16 XmaI C'CCGG_G Cuts at: 0 125 1290 Size: 125 1165 Enzymes that do cut: AcuI AflIII AgeI AlfI AlwNI ApoI AvaI BaeI BanI BanII BbvCI BcgI BglI BlpI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgZI BtsI ClaI DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII HincII MmeI MscI MslI MspA1I NaeI NgoMIV PmlI PpuMI PshAI PstI PvuII SacI SbfI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BbsI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I BtgI CspCI DraI DrdI EagI EarI EcoRI EcoRV FseI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI