Restriction map for
pFN21AE0921
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI Asp700I
AspEI AspI AvaI AvaII AviII
AvrII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
FauI FbaI Fnu4HI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI ItaI KasI KspI MaeII
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI TseI TspRI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 34 GGTACC G^GTACC
AciI 148 CCGC CCGC(-3/-1)
AcsI 657 AAATTT [GA]^AATT[CT]
AflIII 208 ACATGT A^C[GA][CT]GT
AgeI 31 ACCGGT A^CCGGT
ApaI 228 GGGCCC GGGCC^C
ApoI 657 AAATTT [GA]^AATT[CT]
AseI 508 ATTAAT AT^TAAT
AsnI 508 ATTAAT AT^TAAT
Asp718I 34 GGTACC G^GTACC
AspHI 355 GAGCTC G[AT]GC[AT]^C
BalI 171 TGGCCA TGG^CCA
BamHI 238 GGATCC G^GATCC
BbsI 303 GTCTTC GAAGAC(2/6)
BcnI 225 CCGGG CC^[GC]GG
BfaI 769 CTAG C^TAG
BpuAI 303 GTCTTC GAAGAC(2/6)
BsaWI 31 ACCGGT [AT]^CCGG[AT]
BseRI 716 CTCCTC GAGGAG(10/8)
BsiHKAI 355 GAGCTC G[AT]GC[AT]^C
BspHI 729 TCATGA T^CATGA
BspLU11I 208 ACATGT A^CATGT
BsrDI 786 CATTGC GCAATG(2/0)
BsrFI 31 ACCGGT [GA]^CCGG[CT]
BstEII 601 GGTCACC G^GT[ACGT]ACC
BstPI 601 GGTCACC G^GT[ACGT]ACC
BstYI 238 GGATCC [GA]^GATC[CT]
Cfr10I 31 ACCGGT [GA]^CCGG[CT]
DdeI 576 CTTAG C^T[ACGT]AG
DrdI 763 GACTGTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 171 TGGCCA [CT]^GGCC[GA]
EarI 352 GAAGAG CTCTTC(1/4)
EcoO65I 601 GGTCACC G^GT[ACGT]ACC
EcoT22I 445 ATGCAT ATGCA^T
HgaI 301 GCGTC GACGC(5/10)
HincII 526 GTTGAC GT[CT]^[GA]AC
HindII 526 GTTGAC GT[CT]^[GA]AC
KpnI 34 GGTACC GGTAC^C
Ksp632I 352 GAAGAG CTCTTC(1/4)
MaeI 769 CTAG C^TAG
MflI 238 GGATCC [GA]^GATC[CT]
MluNI 171 TGGCCA TGG^CCA
MscI 171 TGGCCA TGG^CCA
MseI 509 TTAA T^TAA
MspA1I 628 CAGCTG C[AC]G^C[GT]G
NciI 225 CCGGG CC^[GC]GG
NsiI 445 ATGCAT ATGCA^T
NspI 208 ACATGT [GA]CATG^[CT]
PinAI 31 ACCGGT A^CCGGT
PshBI 508 ATTAAT AT^TAAT
PvuII 628 CAGCTG CAG^CTG
RcaI 729 TCATGA T^CATGA
SacI 355 GAGCTC GAGCT^C
SapI 352 GAAGAGC GCTCTTC(1/4)
ScaI 279 AGTACT AGT^ACT
SmlI 673 CTTGAG C^T[CT][GA]AG
StuI 370 AGGCCT AGG^CCT
Tru9I 509 TTAA T^TAA
Tsp45I 602 GTCAC ^GT[GC]AC
XhoII 238 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 239 GATCC GGATC(4/5)
238 GGATC
BanI 34 GGTACC G^G[CT][GA]CC
63 GGTGCC
BanII 228 GGGCCC G[GA]GC[CT]^C
355 GAGCTC
BsaJI 225 CCGGGG C^C[ACGT][ACGT]GG
390 CCCTGG
BsiYI 385 CCGTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
401 CCTGTAAATGG
BslI 385 CCGTGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
401 CCTGTAAATGG
BstNI 391 CCTGG CC^[AT]GG
517 CCTGG
Cfr13I 228 GGGCC G^G[ACGT]CC
229 GGCCC
DpnII 239 GATC ^GATC
722 GATC
DraII 227 GGGGCCC [GA]G^G[ACGT]CC[CT]
228 GGGCCCT
EcoO109I 227 GGGGCCC [GA]G^G[ACGT]CC[CT]
228 GGGCCCT
EcoRII 391 CCTGG ^CC[AT]GG
517 CCTGG
HapII 32 CCGG C^CGG
225 CCGG
HinfI 108 GAGTC G^A[ACGT]TC
714 GACTC
HpaII 32 CCGG C^CGG
225 CCGG
HphI 534 GGTGA GGTGA(8/7)
603 TCACC
MboI 239 GATC ^GATC
722 GATC
MspI 32 CCGG C^CGG
225 CCGG
MvaI 391 CCTGG CC^[AT]GG
517 CCTGG
MwoI 188 GCAACTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
812 GCAATTTAGGC
NdeII 239 GATC ^GATC
722 GATC
PleI 714 GACTC GAGTC(4/5)
108 GAGTC
Sau3AI 239 GATC ^GATC
722 GATC
Sau96I 228 GGGCC G^G[ACGT]CC
229 GGCCC
TaqI 40 TCGA T^CGA
720 TCGA
TthHB8I 40 TCGA T^CGA
720 TCGA
XcmI 92 CCAACAATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
242 CCATTCGCAACTTGG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI AluI BmyI Bsp1286I BsrI
BssKI BstF5I Cac8I FokI HaeIII
MaeIII MboII MnlI NlaIII NlaIV
RsaI ScrFI SfaNI SfcI Tsp509I