Restriction map for pFN21AE0921
 (Linear) (Six-base) MAPSORT of: KIEE0921.seq  Check: 9444  from: 1  to: 846



KIEE0921.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

Acc65I G'GTAC_C

Cuts at:      0      34     846 
   Size:         34     812
 
AflIII A'CryG_T

Cuts at:      0     208     846 
   Size:        208     638
 
AgeI A'CCGG_T

Cuts at:      0      31     846 
   Size:         31     815
 
ApaI G_GGCC'C

Cuts at:      0     232     846 
   Size:        232     614
 
ApoI r'AATT_y

Cuts at:      0     657     846 
   Size:        657     189
 
AseI AT'TA_AT

Cuts at:      0     509     846 
   Size:        509     337
 
BamHI G'GATC_C

Cuts at:      0     238     846 
   Size:        238     608
 
BanI G'GyrC_C

Cuts at:      0      34      63     846 
   Size:         34      29     783

  Fragments arranged by size: 

                 783      34      29
 
BanII G_rGCy'C

Cuts at:      0     232     359     846 
   Size:        232     127     487

  Fragments arranged by size: 

                 487     232     127
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     296     846 
   Size:        296     550
 
BfrBI ATG'CAT

Cuts at:      0     447     846 
   Size:        447     399
 
Bme1580I G_kGCm'C

Cuts at:      0     232     391     846 
   Size:        232     159     455

  Fragments arranged by size: 

                 455     232     159
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     540     846 
   Size:        540     306
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     694     846 
   Size:        694     152
 
BsaWI w'CCGG_w

Cuts at:      0      31     846 
   Size:         31     815
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     707     846 
   Size:        707     139
 
BsiHKAI G_wGCw'C

Cuts at:      0     359     846 
   Size:        359     487
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     359     391     846 
   Size:        232     127      32     455

  Fragments arranged by size: 

                 455     232     127      32
 
BspHI T'CATG_A

Cuts at:      0     729     846 
   Size:        729     117
 
BsrDI GCAATG_nn'

Cuts at:      0     785     846 
   Size:        785      61
 
BsrFI r'CCGG_y

Cuts at:      0      31     846 
   Size:         31     815
 
BstEII G'GTnAC_C

Cuts at:      0     601     846 
   Size:        601     245
 
BstYI r'GATC_y

Cuts at:      0     238     846 
   Size:        238     608
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     769     846 
   Size:        769      77
 
EaeI y'GGCC_r

Cuts at:      0     171     846 
   Size:        171     675
 
EarI CTCTTCn'nnn_

Cuts at:      0     347     846 
   Size:        347     499
 
EcoICRI GAG'CTC

Cuts at:      0     357     846 
   Size:        357     489
 
EcoO109I rG'GnC_Cy

Cuts at:      0     228     229     846 
   Size:        228       1     617

  Fragments arranged by size: 

                 617     228       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     846 
   Size:        159      32     655

  Fragments arranged by size: 

                 655     159      32
 
HincII GTy'rAC

Cuts at:      0     528     846 
   Size:        528     318
 
KpnI G_GTAC'C

Cuts at:      0      38     846 
   Size:         38     808
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     241     846 
   Size:        116     125     605

  Fragments arranged by size: 

                 605     125     116
 
MscI TGG'CCA

Cuts at:      0     173     846 
   Size:        173     673
 
MspA1I CmG'CkG

Cuts at:      0     630     846 
   Size:        630     216
 
NsiI A_TGCA'T

Cuts at:      0     449     846 
   Size:        449     397
 
NspI r_CATG'y

Cuts at:      0     212     846 
   Size:        212     634
 
PciI A'CATG_T

Cuts at:      0     208     846 
   Size:        208     638
 
PspOMI G'GGCC_C

Cuts at:      0     228     846 
   Size:        228     618
 
PvuII CAG'CTG

Cuts at:      0     630     846 
   Size:        630     216
 
SacI G_AGCT'C

Cuts at:      0     359     846 
   Size:        359     487
 
SapI GCTCTTCn'nnn_

Cuts at:      0     347     846 
   Size:        347     499
 
ScaI AGT'ACT

Cuts at:      0     281     846 
   Size:        281     565
 
SfcI C'TryA_G

Cuts at:      0      17     472     500     846 
   Size:         17     455      28     346

  Fragments arranged by size: 

                 455     346      28      17
 
SmlI C'TyrA_G

Cuts at:      0     673     846 
   Size:        673     173
 
StuI AGG'CCT

Cuts at:      0     372     846 
   Size:        372     474
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     522     846 
   Size:        522     324
 
TatI w'GTAC_w

Cuts at:      0     279     846 
   Size:        279     567
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     198     230     846 
   Size:        198      32     616

  Fragments arranged by size: 

                 616     198      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      99     249     846 
   Size:         99     150     597

  Fragments arranged by size: 

                 597     150      99
 

 Enzymes that do cut:

   Acc65I   AflIII     AgeI     ApaI     ApoI     AseI    BamHI     BanI
    BanII     BbsI    BfrBI Bme1580I     BmrI    BpuEI    BsaWI    BseRI
  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrFI   BstEII    BstYI     DrdI
     EaeI     EarI  EcoICRI EcoO109I    Hin4I   HincII     KpnI     MmeI
     MscI   MspA1I     NsiI     NspI     PciI   PspOMI    PvuII     SacI
     SapI     ScaI     SfcI     SmlI     StuI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EciI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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