################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AccI AccII AccIII AciI AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AspEI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HpaI KasI KspI MamI MluI MroI MslI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AatII 6 GACGTC GACGT^C Acc65I 999 GGTACC G^GTACC AcyI 6 GACGTC G[GA]^CG[CT]C AflIII 1588 ACATGT A^C[GA][CT]GT Alw44I 1067 GTGCAC G^TGCAC AlwNI 245 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1067 GTGCAC G^TGCAC AseI 1074 ATTAAT AT^TAAT AsnI 1074 ATTAAT AT^TAAT Asp700I 839 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 999 GGTACC G^GTACC AspI 1239 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1388 CTCGAG C^[CT]CG[GA]G AvaII 22 GGACC G^G[AT]CC BalI 1374 TGGCCA TGG^CCA BanI 999 GGTACC G^G[CT][GA]CC BcgI 5 CGACGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 288 GGATAC GTATCC(6/5) BcnI 51 CCGGG CC^[GC]GG BglII 465 AGATCT A^GATCT BsaAI 1519 CACGTA [CT]AC^GT[GA] BsaHI 6 GACGTC G[GA]^CG[CT]C BsgI 1400 GTGCAG GTGCAG(16/14) BsmBI 8 CGTCTC CGTCTC(1/5) BsoBI 1388 CTCGAG C^[CT]CG[GA]G Bsp1407I 91 TGTACA T^GTACA BspLU11I 1588 ACATGT A^CATGT BsrDI 991 GCAATG GCAATG(2/0) BsrGI 91 TGTACA T^GTACA BsrI 1255 ACTGG ACTGG(1/-1) CfoI 1537 GCGC GCG^C DraII 21 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1519 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 271 GAAGAG CTCTTC(1/4) EcoO109I 21 GGGACCC [GA]G^G[ACGT]CC[CT] HapII 51 CCGG C^CGG HhaI 1537 GCGC GCG^C Hin1I 6 GACGTC G[GA]^CG[CT]C HinP1I 1537 GCGC G^CGC HindIII 562 AAGCTT A^AGCTT HpaII 51 CCGG C^CGG KpnI 999 GGTACC GGTAC^C Ksp632I 271 GAAGAG CTCTTC(1/4) MfeI 104 CAATTG C^AATTG MluNI 1374 TGGCCA TGG^CCA MscI 1374 TGGCCA TGG^CCA MspA1I 34 CAGCTG C[AC]G^C[GT]G MspI 51 CCGG C^CGG MunI 104 CAATTG C^AATTG NciI 51 CCGGG CC^[GC]GG NdeI 130 CATATG CA^TATG NspI 1588 ACATGT [GA]CATG^[CT] PaeR7I 1388 CTCGAG C^TCGAG PflFI 1239 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 21 GGGACCC [GA]G^G[AT]CC[CT] PshBI 1074 ATTAAT AT^TAAT PvuII 34 CAGCTG CAG^CTG SspBI 91 TGTACA T^GTACA TaqI 1389 TCGA T^CGA Tth111I 1239 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1389 TCGA T^CGA XcmI 26 CCACGAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1388 CTCGAG C^TCGAG XmnI 839 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 39 GGATC GGATC(4/5) 1258 GGATC AspHI 1067 GTGCAC G[AT]GC[AT]^C 1301 GAGCAC BpmI 203 CTCCAG CTGGAG(16/14) 864 CTCCAG BsaI 389 GGTCTC GGTCTC(1/5) 932 GGTCTC BseRI 1382 CTCCTC GAGGAG(10/8) 1385 CTCCTC BsiHKAI 1067 GTGCAC G[AT]GC[AT]^C 1301 GAGCAC BstYI 39 GGATCT [GA]^GATC[CT] 465 AGATCT EaeI 348 TGGCCG [CT]^GGCC[GA] 1374 TGGCCA EcoNI 146 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 394 CCTGAGTTAGG EcoRI 208 GAATTC G^AATTC 644 GAATTC HincII 264 GTCAAC GT[CT]^[GA]AC 1229 GTCAAC HindII 264 GTCAAC GT[CT]^[GA]AC 1229 GTCAAC MaeII 7 ACGT A^CGT 1520 ACGT MflI 39 GGATCT [GA]^GATC[CT] 465 AGATCT PflMI 1492 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1518 CCACGTAGTGG PstI 797 CTGCAG CTGCA^G 1234 CTGCAG SmlI 150 CTTGAG C^T[CT][GA]AG 1388 CTCGAG SpeI 1087 ACTAGT A^CTAGT 1581 ACTAGT Van91I 1492 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1518 CCACGTAGTGG XhoII 39 GGATCT [GA]^GATC[CT] 465 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI