(Circular) (Six-base) MAPSORT of: pFN21AE5618.seq Check: 1672 from: 1 to: 6304 pFN21AE5618 6304 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3059 3059 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2015 4114 278 Size: 53 83 186 936 479 2099 2468 Fragments arranged by size: 2468 2099 936 479 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3004 3625 3004 Size: 621 5683 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2295 3531 4367 5751 6019 1632 Size: 510 153 1236 836 1384 268 1917 Fragments arranged by size: 1917 1384 1236 836 510 268 153 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 3593 5203 6180 1203 Size: 2390 1610 977 1327 Fragments arranged by size: 2390 1610 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6304 AlwNI CAG_nnn'CTG Cuts at: 2255 4047 5619 2255 Size: 1792 1572 2940 Fragments arranged by size: 2940 1792 1572 ApaLI G'TGCA_C Cuts at: 3072 4361 5517 3072 Size: 1289 1156 3859 Fragments arranged by size: 3859 1289 1156 ApoI r'AATT_y Cuts at: 1493 2213 2649 2848 3036 3613 3839 3991 6100 Size: 720 436 199 188 577 226 152 2109 Cuts at: 6100 1493 Size: 1697 Fragments arranged by size: 2109 1697 720 577 436 226 199 188 152 AseI AT'TA_AT Cuts at: 160 1032 3080 4858 160 Size: 872 2048 1778 1606 Fragments arranged by size: 2048 1778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 3393 3629 1870 Size: 73 1450 236 4545 Fragments arranged by size: 4545 1450 236 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3327 3360 3327 Size: 33 6271 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 3625 5080 618 Size: 325 217 627 1217 621 1455 1842 Fragments arranged by size: 1842 1455 1217 627 621 325 217 BanII G_rGCy'C Cuts at: 1391 3623 1391 Size: 2232 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1999 2033 4495 4529 1903 Size: 34 62 34 2462 34 3678 Fragments arranged by size: 3678 2462 62 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2287 4196 5406 2287 Size: 1909 1210 3185 Fragments arranged by size: 3185 1909 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2470 6299 2470 Size: 3829 2475 BlpI GC'TnA_GC Cuts at: 3721 3721 Size: 6304 Bme1580I G_kGCm'C Cuts at: 1790 2922 3076 4365 5521 1790 Size: 1132 154 1289 1156 2573 Fragments arranged by size: 2573 1289 1156 1132 154 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4035 4993 442 Size: 416 53 83 399 24 2618 958 1753 Fragments arranged by size: 2618 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2854 4964 1239 Size: 954 661 2110 2579 Fragments arranged by size: 2579 2110 954 661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2176 4429 5294 5592 2176 Size: 2253 865 298 2888 Fragments arranged by size: 2888 2253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2400 2943 4967 882 Size: 617 901 543 2024 2219 Fragments arranged by size: 2219 2024 901 617 543 BsaAI yAC'GTr Cuts at: 493 1204 3526 4095 493 Size: 711 2322 569 2702 Fragments arranged by size: 2702 2322 711 569 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 4111 4493 5128 Size: 53 83 186 936 479 2099 382 635 Cuts at: 5128 275 Size: 1451 Fragments arranged by size: 2099 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4737 5135 5409 5556 5967 4737 Size: 398 274 147 411 5074 Fragments arranged by size: 5074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2959 2989 1178 Size: 30 165 30 1556 30 4493 Fragments arranged by size: 4493 1556 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3378 3381 1186 Size: 2192 3 4109 Fragments arranged by size: 4109 2192 3 BseYI C'CCAG_C Cuts at: 2370 3211 5507 6003 2370 Size: 841 2296 496 2671 Fragments arranged by size: 2671 2296 841 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3426 1163 Size: 573 1690 4041 Fragments arranged by size: 4041 1690 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3076 3310 4365 4450 5521 3076 Size: 234 1055 85 1071 3859 Fragments arranged by size: 3859 1071 1055 234 85 BsmI GAATG_Cn' Cuts at: 3815 3908 3815 Size: 93 6211 BsmBI CGTCTCn'nnnn_ Cuts at: 2019 2019 Size: 6304 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2922 3076 3310 3623 4365 4450 5521 Size: 399 1132 154 234 313 742 85 1071 Cuts at: 5521 1391 Size: 2174 Fragments arranged by size: 2174 1132 1071 742 399 313 234 154 85 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 2992 3059 3659 844 Size: 1315 833 67 600 3489 Fragments arranged by size: 3489 1315 833 600 67 BsrBI CCG'CTC Cuts at: 1394 1823 4191 1394 Size: 429 2368 3507 Fragments arranged by size: 3507 2368 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3003 4799 4973 65 Size: 1183 1755 1796 174 1396 Fragments arranged by size: 1796 1755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4948 5135 1700 Size: 110 3138 187 2869 Fragments arranged by size: 3138 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2096 96 Size: 2000 4304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6304 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstYI r'GATC_y Cuts at: 1972 2044 2470 3634 4001 4386 4403 5855 5941 Size: 72 426 1164 367 385 17 1452 86 Cuts at: 5941 5953 6299 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1164 426 385 367 346 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2289 2613 3331 513 Size: 552 939 57 228 324 718 3486 Fragments arranged by size: 3486 939 718 552 324 228 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 2845 2949 3447 3820 4614 4634 2845 Size: 104 498 373 794 20 4515 Fragments arranged by size: 4515 794 498 373 104 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DraIII CAC_nnn'GTG Cuts at: 3529 3529 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2353 3379 3751 Size: 54 1425 50 275 18 523 1026 372 Cuts at: 3751 4640 6269 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1026 889 523 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 4234 1353 Size: 918 1963 3423 Fragments arranged by size: 3423 1963 918 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4891 5265 5411 387 Size: 1064 138 3302 374 146 1280 Fragments arranged by size: 3302 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2193 2295 2854 3531 4367 4964 Size: 393 510 51 102 559 677 836 597 Cuts at: 4964 5751 6019 1239 Size: 787 268 1524 Fragments arranged by size: 1524 836 787 677 597 559 510 393 268 102 51 EcoNI CCTnn'n_nnAGG Cuts at: 2155 2403 2155 Size: 248 6056 EcoO109I rG'GnC_Cy Cuts at: 2027 2027 Size: 6304 EcoRI G'AATT_C Cuts at: 2213 2649 3613 3991 2213 Size: 436 964 378 4526 Fragments arranged by size: 4526 964 436 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2147 2147 Size: 6304 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 2915 2947 3162 Size: 32 135 32 1190 32 121 32 215 Cuts at: 3162 3194 3376 3408 3416 3448 4945 4977 5019 Size: 32 182 32 8 32 1497 32 42 Cuts at: 5019 5051 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 1190 215 182 135 121 42 32 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 2271 3236 3648 3894 669 Size: 988 281 333 965 412 246 3079 Fragments arranged by size: 3079 988 965 412 333 281 246 HindIII A'AGCT_T Cuts at: 748 2567 748 Size: 1819 4485 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3008 3629 3008 Size: 621 5683 MfeI C'AATT_G Cuts at: 2109 3903 2109 Size: 1794 4510 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2333 3122 3265 5418 5602 1305 Size: 1028 789 143 2153 184 2007 Fragments arranged by size: 2153 2007 1028 789 184 143 MscI TGG'CCA Cuts at: 10 64 1489 1832 3381 10 Size: 54 1425 343 1549 2933 Fragments arranged by size: 2933 1549 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4262 4621 4780 518 Size: 648 3096 359 159 2042 Fragments arranged by size: 3096 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 3720 4397 5545 5790 1606 Size: 435 1679 677 1148 245 2120 Fragments arranged by size: 2120 1679 1148 677 435 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2289 2613 3331 513 Size: 552 939 285 324 718 3486 Fragments arranged by size: 3486 939 718 552 324 285 NdeI CA'TA_TG Cuts at: 387 2136 387 Size: 1749 4555 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 3597 3662 5207 1132 Size: 97 2368 65 1545 2229 Fragments arranged by size: 2368 2229 1545 97 65 PasI CC'CwG_GG Cuts at: 1348 2915 1348 Size: 1567 4737 PciI A'CATG_T Cuts at: 3593 5203 3593 Size: 1610 4694 PflMI CCAn_nnn'nTGG Cuts at: 1108 3503 3529 1108 Size: 2395 26 3883 Fragments arranged by size: 3883 2395 26 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 2027 Size: 6304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspXI vC'TCGA_Gb Cuts at: 3393 3393 Size: 6304 PstI C_TGCA'G Cuts at: 830 2806 3243 3656 830 Size: 1976 437 413 3478 Fragments arranged by size: 3478 1976 437 413 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2041 2041 Size: 6304 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SanDI GG'GwC_CC Cuts at: 2027 2027 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2256 2802 3239 3652 4787 5468 5659 Size: 219 1211 546 437 413 1135 681 191 Cuts at: 5659 826 Size: 1471 Fragments arranged by size: 1471 1211 1135 681 546 437 413 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 3393 4408 5309 5571 820 Size: 197 926 212 1238 1015 901 262 1553 Fragments arranged by size: 1553 1238 1015 926 901 262 212 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 3092 3586 152 Size: 2940 494 2870 Fragments arranged by size: 2940 2870 494 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6304 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 2613 2978 3331 3743 513 Size: 552 939 285 324 365 353 412 3074 Fragments arranged by size: 3074 939 552 412 365 353 324 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4331 4348 4501 4686 5131 325 Size: 1255 2751 17 153 185 445 1498 Fragments arranged by size: 2751 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2096 4550 6262 Size: 274 80 33 51 1443 119 2454 1712 Cuts at: 6262 96 Size: 138 Fragments arranged by size: 2454 1712 1443 274 138 119 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2974 3006 6264 6296 2974 Size: 32 3258 32 2982 Fragments arranged by size: 3258 2982 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3247 1316 Size: 1931 4373 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 3393 1943 Size: 1450 4854 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 2848 4433 2848 Size: 1585 4719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2013 4112 276 Size: 53 83 186 936 479 2099 2468 Fragments arranged by size: 2468 2099 936 479 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI